##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-133_GGACTCCT-GTAAGGAG_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1307432	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.436227658493905	34.0	31.0	34.0	31.0	34.0
2	32.62162621077043	34.0	31.0	34.0	31.0	34.0
3	32.73174972006192	34.0	31.0	34.0	31.0	34.0
4	36.24661779733095	37.0	37.0	37.0	35.0	37.0
5	35.96850161232095	37.0	35.0	37.0	35.0	37.0
6	36.06365837764412	37.0	36.0	37.0	35.0	37.0
7	36.06172481628108	37.0	36.0	37.0	35.0	37.0
8	36.086656132020636	37.0	36.0	37.0	35.0	37.0
9	37.94414164560757	39.0	38.0	39.0	35.0	39.0
10-14	38.1236529318542	39.2	38.2	39.4	35.2	39.4
15-19	39.25145583097247	40.0	39.0	41.0	36.0	41.0
20-24	39.18009823837875	40.0	39.0	41.0	36.0	41.0
25-29	38.98918658867153	40.0	38.8	41.0	35.8	41.0
30-34	38.78695901584174	40.0	38.0	41.0	35.0	41.0
35-39	38.55712159408673	40.0	38.0	41.0	34.4	41.0
40-44	38.31476650410882	40.0	38.0	41.0	33.8	41.0
45-49	38.1317119360701	40.0	38.0	41.0	33.4	41.0
50-54	38.077089592422396	40.0	37.4	41.0	33.2	41.0
55-59	37.68786659650368	39.6	36.4	41.0	33.0	41.0
60-64	37.13370653311224	38.8	35.4	40.6	31.8	41.0
65-69	36.4012404469219	37.6	35.0	40.0	31.0	41.0
70-74	35.816919579756345	36.6	35.0	39.2	31.0	40.8
75-79	34.63870350427403	35.2	33.6	37.4	30.2	39.2
80-84	34.451292457275024	35.0	34.0	36.4	31.0	38.2
85-89	33.839046466661365	35.0	34.0	35.6	30.0	36.8
90-94	33.43145264916264	35.0	34.0	35.0	29.8	36.0
95-99	33.16708234156729	35.0	34.0	35.0	29.0	35.6
100-101	32.81433374737654	35.0	33.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	4.0
14	21.0
15	41.0
16	109.0
17	225.0
18	405.0
19	627.0
20	959.0
21	1415.0
22	1970.0
23	2894.0
24	3960.0
25	5072.0
26	6545.0
27	8323.0
28	11007.0
29	14070.0
30	17511.0
31	22348.0
32	28973.0
33	39516.0
34	57050.0
35	90747.0
36	167441.0
37	322336.0
38	417332.0
39	86447.0
40	82.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.06722185169095	23.583329763995376	10.988181412111683	24.361266972201996
2	15.439269122762193	23.400020348043338	39.87160843632937	21.289102092865104
3	15.951881245066666	27.468579627850627	32.85103928923263	23.728499837850077
4	11.44243065796156	18.275367284876	43.9120351957119	26.37016686145054
5	10.71000250873468	39.198673430052196	38.95414828457618	11.13717577663695
6	25.622059120474333	43.79638864583397	19.302877702243787	11.278674531447908
7	23.889655446707746	33.73590366458829	26.751678098746247	15.62276278995772
8	21.145115004069044	38.48964993972918	24.19261575363002	16.172619302571757
9	22.741985816470763	14.934161011815528	25.335313805995263	36.988539365718445
10-14	20.470280672340895	25.90080401887058	32.770775076638785	20.85814023214974
15-19	19.718290252701394	31.73314458451881	28.65713958351148	19.891425579268308
20-24	19.67444981106353	30.0518821902863	30.31878879959028	19.954879199059892
25-29	19.709937648819018	30.596249137283827	29.752573537230404	19.941239676666754
30-34	19.54394085329959	30.325565086704355	29.885205002649656	20.2452890573464
35-39	19.734767056903557	30.558243464173568	29.726293449428542	19.980696029494336
40-44	19.446409836705364	30.294854753558177	30.173717208495088	20.085018201241372
45-49	19.641005799629195	30.0959340151757	30.138420347157414	20.124639838037695
50-54	19.370907373977538	29.73967129665412	30.57949709627777	20.309924233090573
55-59	19.54091449511812	29.7282763144182	30.55255140088659	20.178257789577085
60-64	19.152695034646893	29.35818879591048	30.80764183033545	20.68147433910718
65-69	19.526335362093057	29.53519620101096	30.16029089261857	20.778177544277412
70-74	19.410747938269473	29.29409718392446	30.102451282824806	21.192703594981264
75-79	19.62438443238057	29.60959795969317	29.622493672992857	21.143523934933402
80-84	19.461309180077848	29.44828361616577	29.452643317533877	21.6377638862225
85-89	19.48893621669185	29.29685169485173	29.40473134570839	21.80948074274803
90-94	19.36254424247746	28.796752999508797	29.833346769696806	22.00735598831694
95-99	19.426978473448894	28.687285614480167	29.498468486370726	22.387267425700212
100-101	19.33045823050768	27.90096460120473	30.392184982540194	22.3763921857474
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.42848882389295
2	36.7283712156273
3	39.68038108291674
4	37.8125975194121
5	21.84717828537163
6	36.90073365192224
7	39.51241823666546
8	37.3177343066408
9	59.73052518218921
10-14	41.328420904490635
15-19	39.60971583196971
20-24	39.62932901012342
25-29	39.65117732548577
30-34	39.78922991064599
35-39	39.7154630863979
40-44	39.53142803794674
45-49	39.765645637666886
50-54	39.68083160706811
55-59	39.71917228469521
60-64	39.83416937375407
65-69	40.30451290637047
70-74	40.603451533250734
75-79	40.76790836731397
80-84	41.09907306630035
85-89	41.298416959439884
90-94	41.3699002307944
95-99	41.81424589914911
100-101	41.70685041625507
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	1.0
5	1.5
6	3.0
7	7.5
8	9.0
9	10.0
10	16.5
11	32.0
12	53.5
13	88.0
14	136.0
15	196.5
16	300.0
17	473.0
18	757.5
19	1112.0
20	1632.0
21	2475.5
22	3589.0
23	5064.5
24	6838.0
25	8935.0
26	11648.5
27	14644.5
28	18014.0
29	21881.0
30	25855.0
31	30121.5
32	34687.0
33	39724.0
34	45513.5
35	52582.5
36	61321.0
37	69011.0
38	73871.0
39	75257.0
40	72578.0
41	67693.0
42	61559.5
43	55711.5
44	51547.5
45	47761.5
46	44007.0
47	39934.5
48	35441.5
49	30798.5
50	26644.5
51	23549.0
52	20931.0
53	18847.0
54	16532.5
55	14375.5
56	12996.0
57	11068.5
58	9010.0
59	7712.5
60	6434.5
61	5305.5
62	4290.0
63	3108.0
64	2297.5
65	1926.5
66	1585.5
67	1260.0
68	1075.5
69	1035.5
70	977.0
71	792.5
72	675.0
73	579.5
74	449.5
75	353.5
76	255.5
77	172.5
78	104.5
79	63.5
80	42.0
81	28.0
82	21.5
83	11.0
84	9.0
85	8.0
86	3.5
87	2.0
88	1.5
89	1.0
90	0.5
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.013843932227450452
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0011778815265344584
20-24	6.424808326551591E-4
25-29	0.01708693071609078
30-34	0.007097883484571282
35-39	0.042113088864277455
40-44	0.04994523615759748
45-49	0.0503888538753832
50-54	0.05055712266488812
55-59	0.04116466477797698
60-64	0.05178089567946938
65-69	0.042679083883521284
70-74	0.004543257316632911
75-79	0.001590904918955632
80-84	2.2945744023398538E-4
85-89	0.0037631020198373604
90-94	0.002646409144031965
95-99	0.002493437517209308
100-101	3.8242906705664235E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1307432.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	30.633522167276592
#Duplication Level	Relative count
1	100.0
2	7.589189189189189
3	3.3241698841698843
4	2.291274131274131
5	1.7303474903474902
6	1.3306563706563705
7	1.092818532818533
8	0.8605405405405405
9	0.6826254826254826
10++	4.650501930501931
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	179405	2.8656812	6.5153575	85-89
GAGCC	175280	2.799791	6.3818736	95-97
CTCCG	184900	2.7581954	6.2661514	95-97
AGAAG	346105	2.7307184	5.068577	5
CGAGC	167185	2.6704879	6.2690563	85-89
GCCCA	164675	2.4952838	6.0850973	90-94
AGCCC	164100	2.486571	5.988112	90-94
ACACA	332580	2.3613648	6.898677	6
ATCTC	329745	2.2690125	5.1303687	95-97
CCCAC	155115	2.2296932	5.633135	90-94
CTTTG	297350	2.1233592	8.617505	9
CCACG	139285	2.1105547	5.716259	90-94
TACAC	301805	2.1095471	9.058638	5
GACTC	200585	2.1029282	5.4083996	7
GACGG	121300	2.0424676	5.9490957	95-97
CGGAC	114290	1.8255829	5.509944	90-94
GGACT	158870	1.7557752	7.260515	6
GAGAG	143810	1.7018498	5.531085	7
TGGCT	147455	1.6042882	5.1695185	6
TTGAG	202935	1.5517354	5.460905	9
TATAC	305670	1.4782549	5.826719	5
GAGTC	132790	1.4675481	7.399831	9
AAGAC	185955	1.3917968	5.733585	5
CCATG	131820	1.3819977	5.865877	9
GTCTT	192120	1.3719178	6.2395697	1
TATGA	261940	1.3353629	6.3251224	4
TGAGT	171530	1.3115982	5.3533926	8
GACTT	178780	1.2968168	6.7137747	7
TCATA	265350	1.283263	6.216567	2
GATTG	166185	1.2707278	6.1690063	7
GTGTT	168590	1.2690783	5.975161	1
GTATG	158310	1.2105119	5.735248	3
GTGTA	156250	1.1947602	10.896941	1
GTTCT	164985	1.1781484	5.270047	1
TGGAC	101565	1.1224606	7.3462486	5
ACATG	152035	1.1202306	5.315791	8
ACACC	109905	1.1103159	5.231202	6
GTCCA	104960	1.100398	7.803307	1
ACACT	147135	1.0284396	5.0529103	6
TGTAT	203695	1.0222893	5.16883	2
GAGTA	129690	1.007329	5.408335	1
AGACT	135700	0.99987036	5.5051303	6
GTATA	155225	0.7913329	7.2008266	1
>>END_MODULE