##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-131_GGACTCCT-TATCCTCT_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1727814	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.620942416255453	33.0	31.0	34.0	30.0	34.0
2	31.820776426166244	33.0	31.0	34.0	30.0	34.0
3	31.961979703833862	34.0	31.0	34.0	30.0	34.0
4	35.48903817193286	37.0	35.0	37.0	33.0	37.0
5	35.357634560201504	37.0	35.0	37.0	33.0	37.0
6	35.49645621577323	37.0	35.0	37.0	33.0	37.0
7	35.508047741249925	37.0	35.0	37.0	33.0	37.0
8	35.558264952130266	37.0	35.0	37.0	33.0	37.0
9	37.3474963161544	39.0	37.0	39.0	34.0	39.0
10-14	37.46838074005651	39.2	37.2	39.4	34.0	39.4
15-19	38.506735447218276	40.0	38.0	41.0	34.0	41.0
20-24	38.39527807970071	40.0	38.0	41.0	34.0	41.0
25-29	38.19985148864403	40.0	38.0	41.0	33.2	41.0
30-34	37.92338318823669	40.0	38.0	41.0	32.8	41.0
35-39	37.592804086550984	40.0	37.4	41.0	31.6	41.0
40-44	37.52881606469215	40.0	37.0	41.0	31.8	41.0
45-49	37.34443325496842	40.0	36.8	41.0	31.2	41.0
50-54	36.644123499404444	38.8	35.4	40.2	30.6	40.6
55-59	37.26079971571014	39.8	36.0	41.0	31.0	41.0
60-64	36.80212881710648	39.0	35.0	41.0	31.0	41.0
65-69	36.1338842028135	37.8	35.0	40.2	30.0	41.0
70-74	35.31606446064218	36.4	34.4	39.2	29.0	41.0
75-79	34.382872924979196	35.4	34.0	37.6	28.6	39.4
80-84	33.48685610835426	35.0	33.4	36.4	27.4	38.2
85-89	32.73045848685101	35.0	33.0	35.4	26.0	36.6
90-94	32.16556041333153	35.0	32.4	35.0	25.2	36.0
95-99	31.693403109362464	35.0	32.0	35.0	24.0	35.2
100-101	30.849179367686567	34.0	30.0	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	2.0
6	47.0
7	172.0
8	408.0
9	513.0
10	635.0
11	959.0
12	1473.0
13	1988.0
14	2393.0
15	2997.0
16	3471.0
17	3986.0
18	4356.0
19	4982.0
20	5626.0
21	6310.0
22	7182.0
23	8316.0
24	9564.0
25	11071.0
26	13088.0
27	15390.0
28	18175.0
29	22012.0
30	27573.0
31	34689.0
32	45108.0
33	60824.0
34	86152.0
35	132242.0
36	223239.0
37	395635.0
38	478712.0
39	98429.0
40	95.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.83738750083226	23.3208567450289	10.434248958595676	24.407506795543167
2	15.281897134163039	23.597056410689877	39.76086086845264	21.36018558669445
3	15.770844417376384	27.834876077745285	32.70556401110846	23.688715493769873
4	11.220976714216988	18.410374657787624	43.99584800646998	26.37280062152541
5	10.909603545040753	38.83936273561911	38.34123217261116	11.909801546728978
6	25.627943803095533	43.45762370484239	19.482434705304257	11.43199778675782
7	23.887806588949427	33.77881261857551	26.74154793199684	15.591832860478227
8	21.170024291582333	38.30540023232407	24.35530649818638	16.16926897790722
9	22.941566968995957	15.121364828363355	25.21485327933039	36.7222149233103
10-14	20.606496991361887	25.7982094030649	32.68361490358235	20.911678701990862
15-19	19.96955005282429	31.510195811685023	28.528439729655414	19.991814405835274
20-24	19.835580113362845	29.908474751308205	30.192518626558602	20.06342650877035
25-29	19.85202960102513	30.461517625528277	29.64155117150467	20.044901601941916
30-34	19.71677585131965	30.144294134356738	29.758318263867938	20.38061175045567
35-39	19.910457308804094	30.41489201731631	29.608808689317584	20.065841984562013
40-44	19.65422618931162	30.234862193719604	30.023064476226384	20.08784714074239
45-49	19.839529725549756	30.00794132219542	30.014169356220393	20.138359596034434
50-54	19.58855852172411	29.7533143086545	30.30405144164497	20.35407572797642
55-59	19.804058344930382	29.76430233551507	30.350096901221413	20.081542418333143
60-64	19.50762353257692	29.356148460319275	30.640515191440056	20.49571281566375
65-69	19.85738875058348	29.536050942082138	30.16368121466729	20.442879092667095
70-74	19.85385706236163	29.330167011072906	30.168249847092426	20.647726079473035
75-79	20.049319443997472	29.7875596552964	29.70216167896334	20.460959221742787
80-84	20.18281978818549	29.72329408561396	29.499460762545198	20.59442536365535
85-89	20.30471849217922	29.67286960765746	29.439281115149473	20.583130785013847
90-94	20.421672597127333	29.354281131966474	29.76995940460081	20.454086866305378
95-99	20.733520300561434	29.334572321148052	29.269315927306437	20.662591450984078
100-101	20.92497218855523	28.779086256573745	29.840551575325897	20.45538997954513
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	66.24489429637542
2	36.64208272085749
3	39.45955991114626
4	37.5937773357424
5	22.819405091769728
6	37.05994158985335
7	39.47963944942766
8	37.33929326948955
9	59.663781892306254
10-14	41.51817569335275
15-19	39.96136445865957
20-24	39.89900662213319
25-29	39.896931202967046
30-34	40.097387601775324
35-39	39.97629929336611
40-44	39.74207333005401
45-49	39.977889321584186
50-54	39.94263424970053
55-59	39.885600763263525
60-64	40.003336348240666
65-69	40.30026784325057
70-74	40.501583141834665
75-79	40.51027866574026
80-84	40.77724515184084
85-89	40.88784927719307
90-94	40.87575946343271
95-99	41.39611175154551
100-101	41.38036216810036
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	3.5
2	4.5
3	7.0
4	12.5
5	16.0
6	20.5
7	30.0
8	37.0
9	44.0
10	62.5
11	76.0
12	112.5
13	169.0
14	250.5
15	368.0
16	535.5
17	769.0
18	1134.0
19	1648.5
20	2392.5
21	3526.5
22	4968.0
23	6944.0
24	9392.0
25	12291.0
26	15827.5
27	19829.5
28	24332.0
29	29360.0
30	35052.0
31	41035.5
32	47020.5
33	53746.0
34	61826.5
35	71508.0
36	82602.5
37	92027.5
38	97563.0
39	98629.0
40	95205.5
41	88901.5
42	80610.5
43	72720.0
44	66663.0
45	60562.5
46	55065.5
47	49846.5
48	43524.0
49	37111.5
50	31990.0
51	28906.0
52	25963.0
53	22927.5
54	20317.0
55	18193.5
56	16501.0
57	14301.5
58	12041.5
59	10475.5
60	9063.0
61	7720.0
62	6638.0
63	5363.0
64	4405.5
65	3940.0
66	3422.5
67	2970.5
68	2647.0
69	2505.0
70	2362.0
71	2014.0
72	1724.0
73	1416.5
74	1112.5
75	871.5
76	670.5
77	499.5
78	353.5
79	277.5
80	223.0
81	157.5
82	107.0
83	80.0
84	57.0
85	43.0
86	31.5
87	22.0
88	20.0
89	18.0
90	12.5
91	12.0
92	12.0
93	10.0
94	6.5
95	5.0
96	6.5
97	6.0
98	2.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03351055148297213
2	0.030963981076666817
3	0.027838644668928485
4	0.026102346664629412
5	0.03217938967967617
6	0.0018520512045856789
7	6.945192017196296E-4
8	0.0031832130078816353
9	4.0513620100311726E-4
10-14	0.0070609452174829005
15-19	0.022513997455744658
20-24	0.019353935087920344
25-29	0.0020835576051588885
30-34	0.0021761601653881727
35-39	0.0058918378945881904
40-44	0.012860180551841807
45-49	0.00821847722034895
50-54	0.005394099133355789
55-59	0.004352320330776345
60-64	0.010221007585307215
65-69	0.013682028273876703
70-74	0.01735140472296208
75-79	0.00801012145983306
80-84	0.010950252747112825
85-89	0.009283406662985714
90-94	0.010406212705765784
95-99	0.009676967543960172
100-101	0.006250672815476665
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1727814.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	29.77555522614512
#Duplication Level	Relative count
1	100.0
2	7.650042527829342
3	3.076283129799902
4	2.0860365925585613
5	1.6458580377597456
6	1.3230190412923493
7	1.0548143365348202
8	0.9368539339794252
9	0.7443922245469389
10++	5.651544970851364
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GCTGC	208675	2.4857242	5.3346105	90-94
ACACA	406875	2.2430923	6.7318196	6
CTTTG	397085	2.1647546	8.856801	9
GCCGA	177390	2.1326017	5.280163	90-94
CGACG	172370	2.0722508	5.1779003	95-97
TACAC	363205	1.9839917	8.569256	5
GCTTT	339945	1.853249	5.484729	1
ATACA	435230	1.6366012	5.3484344	6
GAGAG	193110	1.6246302	5.0754495	7
GACTC	183050	1.4765735	5.1715555	7
GAGTC	174095	1.4275943	7.8381224	9
CCATG	172815	1.3940129	5.433686	9
AAGAC	248240	1.391205	5.4044404	5
TATAC	365750	1.3627313	5.92809	5
GTGTA	240670	1.3461032	9.756575	1
GTCTT	244805	1.3345826	6.2860184	1
TCATA	351745	1.310551	5.6533422	2
GACTT	235460	1.2955089	6.473191	7
TATGA	341590	1.2937914	5.6716676	4
TGAGT	227055	1.2699524	6.02719	8
AACAC	225530	1.2433416	5.016884	5
GTGTT	223005	1.2358704	5.9572988	1
ATGAG	216545	1.2223698	5.048005	7
GATTG	217455	1.2162583	5.4764247	7
GTTCT	219585	1.1970927	5.289035	1
TGGAC	138025	1.1318172	7.120108	5
GTCCA	138890	1.1203567	8.201588	1
ACACT	200535	1.095414	5.5072293	6
GGACT	127600	1.0463312	7.14988	6
AGACT	179575	0.9971653	5.2968326	6
GTATA	203140	0.7694043	7.240806	1
>>END_MODULE