##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-131_GGACTCCT-TATCCTCT_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1727814	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.43296269158601	34.0	31.0	34.0	31.0	34.0
2	32.62299587802854	34.0	31.0	34.0	31.0	34.0
3	32.72589700048732	34.0	31.0	34.0	31.0	34.0
4	36.24619606045558	37.0	37.0	37.0	35.0	37.0
5	35.96864477310636	37.0	35.0	37.0	35.0	37.0
6	36.06092206684284	37.0	36.0	37.0	35.0	37.0
7	36.06249804666474	37.0	36.0	37.0	35.0	37.0
8	36.08703135869949	37.0	36.0	37.0	35.0	37.0
9	37.93892398140078	39.0	38.0	39.0	35.0	39.0
10-14	38.11874368421601	39.2	38.2	39.4	35.2	39.4
15-19	39.24274811987864	40.0	39.0	41.0	36.0	41.0
20-24	39.16687652721878	40.0	39.0	41.0	36.0	41.0
25-29	38.96523514683872	40.0	38.6	41.0	35.6	41.0
30-34	38.75670691405441	40.0	38.0	41.0	35.0	41.0
35-39	38.51014750430313	40.0	38.0	41.0	34.0	41.0
40-44	38.24751541543245	40.0	38.0	41.0	33.6	41.0
45-49	38.05006464816236	40.0	37.8	41.0	33.0	41.0
50-54	37.980462364583225	40.0	37.0	41.0	33.0	41.0
55-59	37.58700265190582	39.6	36.2	41.0	32.8	41.0
60-64	37.03194047507428	38.8	35.2	40.4	31.8	41.0
65-69	36.2990709648145	37.6	35.0	40.0	31.0	41.0
70-74	35.74107143477249	36.6	34.8	39.0	31.0	40.8
75-79	34.58996234548395	35.2	33.6	37.4	30.0	39.2
80-84	34.418657448081795	35.0	34.0	36.4	31.0	38.2
85-89	33.80342733650728	35.0	34.0	35.6	30.0	36.8
90-94	33.38962480915191	35.0	34.0	35.0	29.4	36.0
95-99	33.1118732687662	35.0	33.8	35.0	29.0	35.6
100-101	32.75901514862132	35.0	33.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	3.0
13	9.0
14	16.0
15	48.0
16	168.0
17	309.0
18	525.0
19	932.0
20	1331.0
21	1986.0
22	2794.0
23	3819.0
24	5207.0
25	6993.0
26	8858.0
27	11704.0
28	14831.0
29	18942.0
30	23745.0
31	30290.0
32	39492.0
33	53761.0
34	77784.0
35	124823.0
36	225820.0
37	421483.0
38	541327.0
39	110708.0
40	105.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.1848150321736	23.450325092863007	10.850126228864912	24.51473364609848
2	15.512586752797217	23.542524065037824	39.48281151417276	21.462077667992197
3	15.889615433142687	27.552560634420143	32.98624736227394	23.571576570163224
4	11.451290474553396	18.453317313090416	43.62506612401567	26.470326088340528
5	10.664805355206058	38.99679016375605	38.4904277891023	11.847976691935589
6	25.579257952534242	43.70227350860683	19.336224848276494	11.382243690582436
7	23.83995036502772	33.8351813331759	26.744892679420357	15.579975622376018
8	21.122586111699523	38.384166351239195	24.24936943444144	16.243878102619842
9	22.80691092906991	15.053761573873114	25.23512368808217	36.904203808974806
10-14	20.53897005117449	25.783527625080016	32.718903770892005	20.95859855285349
15-19	19.871783518962822	31.53922168847884	28.54032702691972	20.048667765638616
20-24	19.750698026311145	29.93486168134487	30.1909908333151	20.123449459028887
25-29	19.83906967816135	30.47446335044741	29.635820530575472	20.050646440815754
30-34	19.700762967842923	30.14469057891165	29.761800163755225	20.392746289490205
35-39	19.84891881134735	30.429095172324665	29.619701883778514	20.10228413254947
40-44	19.65019272627125	30.2066841885704	30.021126811130106	20.121996274028245
45-49	19.79512227436952	30.01058773879161	30.029500205917735	20.16478978092113
50-54	19.570755048579795	29.71436751184803	30.332094628595307	20.382782810976867
55-59	19.72584090833293	29.72992408942514	30.34277181988979	20.201463182352132
60-64	19.38037276909853	29.338721322358154	30.5871644889336	20.693741419609722
65-69	19.722644735478394	29.527525636920636	30.07247413147712	20.677355496123848
70-74	19.64892244427935	29.326307445155635	30.03519735219971	20.989572758365306
75-79	19.761461227089324	29.673370466785766	29.562060398445134	21.00310790767978
80-84	19.668882458910108	29.504701155115836	29.4241251378769	21.402291248097157
85-89	19.6602876795103	29.347766698538454	29.42496509923855	21.566980522712697
90-94	19.53888005989695	28.949973242926102	29.79924265268577	21.711904044491174
95-99	19.64875718077382	28.91425461232358	29.434345962265652	22.00264224463694
100-101	19.561028884491552	28.244347044500767	30.173288428782513	22.02133564222517
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.69954867827208
2	36.974664420789416
3	39.46119200330591
4	37.921616562893924
5	22.51278204714165
6	36.96150164311668
7	39.41992598740374
8	37.366464214319365
9	59.71111473804472
10-14	41.497568604027975
15-19	39.92045128460144
20-24	39.874147485340025
25-29	39.889716118977105
30-34	40.093509257333125
35-39	39.95120294389682
40-44	39.7721890002995
45-49	39.959912055290644
50-54	39.95353785955666
55-59	39.92730409068507
60-64	40.07411418870825
65-69	40.40000023160224
70-74	40.638495202644656
75-79	40.764569134769104
80-84	41.07117370700726
85-89	41.227268202223
90-94	41.250784104388124
95-99	41.65139942541076
100-101	41.58236452671672
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	1.0
5	0.5
6	5.0
7	10.0
8	14.0
9	17.0
10	24.0
11	43.5
12	82.0
13	140.0
14	217.5
15	318.5
16	475.5
17	721.5
18	1114.0
19	1668.0
20	2428.5
21	3537.0
22	5019.0
23	7040.0
24	9475.0
25	12407.0
26	16002.5
27	19813.5
28	24267.0
29	29277.5
30	34612.5
31	40531.5
32	46306.0
33	53116.0
34	61454.5
35	70604.0
36	81525.5
37	91407.0
38	97105.0
39	97869.0
40	94101.5
41	87785.0
42	79469.5
43	71529.5
44	66001.0
45	60888.5
46	55790.0
47	50631.5
48	44973.5
49	38612.5
50	33178.0
51	29832.5
52	26666.0
53	23829.0
54	21191.0
55	18781.5
56	17089.5
57	14855.0
58	12401.5
59	10823.5
60	9314.0
61	8007.5
62	6871.0
63	5377.5
64	4325.0
65	3833.0
66	3417.0
67	2972.5
68	2592.5
69	2482.0
70	2352.5
71	1971.0
72	1650.5
73	1426.5
74	1148.0
75	879.5
76	647.0
77	450.5
78	340.0
79	239.0
80	149.0
81	100.5
82	58.0
83	43.0
84	29.0
85	18.5
86	15.5
87	9.0
88	4.0
89	2.5
90	2.0
91	2.5
92	1.0
93	0.0
94	0.5
95	1.0
96	0.5
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.011806826429233702
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0010070528424934628
20-24	7.986970819775739E-4
25-29	0.01786071880422314
30-34	0.0065632064562504994
35-39	0.042979163266416404
40-44	0.05259825421023328
45-49	0.05271400741051988
50-54	0.0522394192893448
55-59	0.04220361682449616
60-64	0.0527371580505772
65-69	0.04138176910246126
70-74	0.004074512650088493
75-79	0.0016089694839838083
80-84	2.662323606591913E-4
85-89	0.0033568428083115423
90-94	0.0026970495666778947
95-99	0.00234978996581808
100-101	5.7876600143302465E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1727814.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	30.518095784092733
#Duplication Level	Relative count
1	100.0
2	7.967245905738217
3	3.19664958119765
4	2.1621452681585196
5	1.646455806975872
6	1.341417677209651
7	1.1595199399924991
8	0.9494936867108389
9	0.7563445430678835
10++	5.83635454431804
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	213495	2.5518527	5.5154796	95-97
GAGCC	206445	2.4675858	5.302974	95-97
CTCCG	215230	2.4330235	5.238181	95-97
CGAGC	196180	2.3448908	5.1836705	85-89
ACACA	406955	2.1892195	6.6719995	6
CTTTG	396380	2.1559308	8.802822	9
TACAC	364205	1.9374378	8.717884	5
GCTTT	338040	1.8386166	5.343556	1
GACGG	146545	1.8314804	5.080174	95-97
GAGAG	191415	1.6780623	5.189916	7
GGACT	197680	1.6389679	7.2037435	6
ATACA	434680	1.6220056	5.433136	6
TTGAG	270055	1.5531011	5.28129	9
GAGTC	174625	1.4478185	7.8630147	9
CCATG	177790	1.409781	5.4440246	9
AAGAC	250450	1.4087285	5.664418	5
GTCTT	253835	1.3806213	6.2350273	1
TATAC	368085	1.3582188	5.927282	5
TATGA	344745	1.330096	5.7541857	4
GACTT	238840	1.3136847	6.47775	7
TGAGT	228330	1.3131381	6.198683	8
TCATA	351730	1.2978694	5.550489	2
GTGTT	225930	1.2848734	6.0780725	1
GATTG	219980	1.2651172	5.8501306	7
ATGAG	216870	1.2612698	5.27306	7
GTGTA	207565	1.1937176	10.1902275	1
GTTCT	219280	1.192675	5.187944	1
TGGAC	140450	1.164473	7.2278633	5
GTATG	201785	1.1604766	5.295232	3
GTCCA	141010	1.118135	7.7940993	1
ACACT	198890	1.0580223	5.50929	6
AGACT	178880	0.99496317	5.272353	6
GAGTA	170190	0.9897889	5.039656	1
GGATA	152015	0.8840869	5.0283756	1
GTATA	201595	0.7777943	7.229195	1
>>END_MODULE