##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-130_GGACTCCT-CTCTCTAT_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1174790	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.643696320193396	33.0	31.0	34.0	30.0	34.0
2	31.84524553324424	33.0	31.0	34.0	30.0	34.0
3	31.987121102494914	34.0	31.0	34.0	30.0	34.0
4	35.508821151014224	37.0	35.0	37.0	33.0	37.0
5	35.3770026983546	37.0	35.0	37.0	33.0	37.0
6	35.51595944807157	37.0	35.0	37.0	33.0	37.0
7	35.526417487380726	37.0	35.0	37.0	33.0	37.0
8	35.58178738327701	37.0	35.0	37.0	33.0	37.0
9	37.37976234050341	39.0	37.0	39.0	34.0	39.0
10-14	37.50336366499545	39.2	37.2	39.4	34.0	39.4
15-19	38.55297695758391	40.0	38.0	41.0	34.0	41.0
20-24	38.4444847164174	40.0	38.0	41.0	34.0	41.0
25-29	38.259953693851664	40.0	38.0	41.0	33.6	41.0
30-34	37.99446556405826	40.0	38.0	41.0	33.0	41.0
35-39	37.67091701495586	40.0	37.6	41.0	31.8	41.0
40-44	37.62733714110607	40.0	37.4	41.0	31.8	41.0
45-49	37.459991317597186	40.0	37.0	41.0	31.4	41.0
50-54	36.756284272082674	39.0	36.0	40.2	30.8	40.6
55-59	37.37372568714408	40.0	36.2	41.0	31.6	41.0
60-64	36.91046859438708	39.0	35.2	41.0	31.0	41.0
65-69	36.2335036900212	37.8	35.0	40.2	30.4	41.0
70-74	35.39635032644132	36.6	34.4	39.2	29.4	41.0
75-79	34.45293184313792	35.4	34.0	37.6	29.0	39.4
80-84	33.54226661786362	35.0	33.6	36.4	27.6	38.0
85-89	32.779941947071386	35.0	33.0	35.4	26.2	36.6
90-94	32.20796465751325	35.0	32.4	35.0	25.2	36.0
95-99	31.72817082201926	35.0	32.0	35.0	24.0	35.2
100-101	30.86630036006435	34.0	30.0	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	40.0
7	121.0
8	248.0
9	314.0
10	431.0
11	574.0
12	909.0
13	1180.0
14	1601.0
15	1837.0
16	2233.0
17	2384.0
18	2753.0
19	3248.0
20	3625.0
21	4143.0
22	4765.0
23	5509.0
24	6234.0
25	7322.0
26	8563.0
27	10011.0
28	12230.0
29	14700.0
30	18209.0
31	22525.0
32	30091.0
33	40414.0
34	57717.0
35	89010.0
36	152435.0
37	274320.0
38	330332.0
39	64719.0
40	42.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.79517397235312	23.2845871275275	10.574753359639368	24.345485540480013
2	15.312906587829783	23.616238990197612	39.73755287770519	21.33330154426741
3	15.822362315212635	27.6661234373643	32.745147123183635	23.766367124239437
4	11.311409666836672	18.616072454871095	43.84304736864063	26.2294705096516
5	10.89155448757522	39.05603519112525	38.21287190679052	11.839538414509017
6	25.66415523812037	43.32073788500318	19.47924815473419	11.535858722142253
7	24.025155412474028	33.770153824938696	26.585981850172413	15.618708912414867
8	21.212567471974815	38.28560532608298	24.265480141681568	16.236347060260634
9	22.99700624542999	15.195113293649273	25.172309004502125	36.63557145641862
10-14	20.596949515683175	25.903203376290644	32.58953714934447	20.91030995868171
15-19	19.89859542712172	31.598436665310587	28.51737107606041	19.98559683150728
20-24	19.779617055217148	29.949429519755828	30.2140143813741	20.05693904365293
25-29	19.801396638438575	30.511687628047042	29.663568057437256	20.023347676077126
30-34	19.647017543740176	30.20846864389914	29.754642326399132	20.389871485961553
35-39	19.87202613482186	30.52040310252262	29.58434117192979	20.023229590725734
40-44	19.585496268601442	30.261847482010467	30.050609577832383	20.102046671555705
45-49	19.773785054939665	29.991340549655458	30.048479982129457	20.18639441327542
50-54	19.51598506087216	29.675215107062787	30.449905281216978	20.358894550848078
55-59	19.68614907289116	29.67895774824371	30.489922611540443	20.14497056732469
60-64	19.40493448037374	29.26427526245479	30.752271078025238	20.57851917914623
65-69	19.770966695584583	29.42247884153521	30.234115933317952	20.572438529562255
70-74	19.767339890581603	29.17335795352281	30.193231820459697	20.86607033543589
75-79	19.95259378918027	29.75291359892888	29.722693075941876	20.571799535948973
80-84	20.14834587908912	29.648999770316557	29.50354494006011	20.699109410534213
85-89	20.40324842032035	29.63076025695748	29.32172821627169	20.64426310645048
90-94	20.38458087474519	29.294866959997506	29.747956017059114	20.57259614819819
95-99	20.867642942611837	29.19307067910042	29.142283036484702	20.797003341803048
100-101	21.05805499301542	28.553515655924635	29.80686642303642	20.581562928023523
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	66.14065951283314
2	36.64620813209719
3	39.58872943945207
4	37.540880176488265
5	22.731092902084235
6	37.20001396026262
7	39.643864324888895
8	37.44891453223545
9	59.6325777018486
10-14	41.50725947436488
15-19	39.884192258629
20-24	39.836556098870076
25-29	39.824744314515705
30-34	40.036889029701726
35-39	39.8952557255476
40-44	39.68754294015715
45-49	39.96017946821509
50-54	39.87487961172024
55-59	39.83111964021585
60-64	39.98345365951997
65-69	40.34340522514684
70-74	40.633410226017496
75-79	40.524393325129246
80-84	40.84745528962333
85-89	41.04751152677083
90-94	40.957177022943384
95-99	41.664646284414886
100-101	41.63961792103895
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	7.0
1	6.0
2	3.5
3	4.0
4	6.0
5	9.5
6	13.0
7	19.0
8	24.5
9	32.5
10	46.5
11	53.5
12	75.5
13	106.5
14	139.5
15	200.5
16	278.5
17	422.5
18	649.5
19	923.0
20	1302.0
21	1905.5
22	2781.0
23	3960.0
24	5419.5
25	7220.5
26	9482.0
27	12002.5
28	14952.5
29	18370.5
30	22081.0
31	26127.5
32	30208.0
33	35037.0
34	41132.0
35	48274.5
36	56718.0
37	64198.0
38	68991.5
39	70081.5
40	67216.5
41	62711.5
42	57007.0
43	51417.5
44	47224.5
45	43023.0
46	38909.0
47	35081.5
48	31104.0
49	26622.0
50	22885.0
51	20652.5
52	18411.5
53	16362.0
54	14402.0
55	12506.0
56	11072.0
57	9580.5
58	8078.5
59	6810.0
60	5714.0
61	4736.0
62	3853.5
63	2957.0
64	2189.5
65	1773.5
66	1467.5
67	1164.0
68	974.0
69	968.5
70	929.5
71	760.0
72	656.0
73	562.5
74	420.0
75	322.0
76	242.5
77	192.5
78	149.5
79	97.0
80	71.0
81	51.5
82	37.5
83	28.5
84	23.5
85	16.5
86	10.5
87	10.5
88	10.5
89	9.0
90	6.5
91	6.5
92	8.0
93	8.5
94	5.0
95	2.5
96	3.0
97	4.0
98	3.0
99	2.0
100	3.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03472961124967015
2	0.03251644974846568
3	0.028260369938457083
4	0.027324032380255193
5	0.034304003268669295
6	0.0022982830974046424
7	0.0011065807506022351
8	0.0036602286366073933
9	9.363375582018914E-4
10-14	0.008886694643297952
15-19	0.027766664680496086
20-24	0.023050928251006565
25-29	0.002247210139684539
30-34	0.0019918453510840233
35-39	0.0069288979306939965
40-44	0.016190127597272702
45-49	0.010180542905540565
50-54	0.004205006852288494
55-59	0.0035070097634470844
60-64	0.012734190791545722
65-69	0.01710944083623456
70-74	0.021501715200163432
75-79	0.0077971382119357505
80-84	0.011048783186782318
85-89	0.007643919338775441
90-94	0.008358940746856886
95-99	0.008069527319776301
100-101	0.005022174175810145
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1174790.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	29.411941697077538
#Duplication Level	Relative count
1	100.0
2	7.321231754769488
3	3.2237046419154676
4	2.199475097581917
5	1.6282935799928147
6	1.189251070204176
7	0.9298445387617905
8	0.805621692718958
9	0.6375554892492434
10++	3.8521260983674437
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GCTGC	156945	2.7390816	6.2598114	90-94
ACACA	302080	2.4447677	7.347153	6
GCCGA	133535	2.356468	6.0880027	95-97
GACGC	133455	2.355056	6.2049685	95-97
CGCTG	134045	2.3394198	5.8686047	95-97
CTGCC	136140	2.326165	5.8484836	90-94
GAGAG	182130	2.270795	5.0367045	7
CGACG	128095	2.2604692	6.2648787	95-97
CTTTG	275835	2.2056727	8.581795	9
TACAC	272915	2.18441	8.871099	5
CCGAC	124935	2.1584787	5.8542175	95-97
TGCCG	120765	2.1076503	5.7875323	90-94
CACAT	260105	2.081879	5.197474	7
GCTTT	233380	1.8661878	5.3758917	1
ATACA	322150	1.7835786	5.222174	6
GACTC	131245	1.5511895	5.0840607	7
TTGAG	184835	1.5264673	5.1344786	9
TATAC	273660	1.4984304	5.5083117	5
GAGTC	123900	1.4957402	8.658807	9
GTGTA	171915	1.419767	10.102852	1
CCATG	119010	1.4065837	5.9037004	9
AAGAC	170070	1.4058728	5.2846746	5
GTCTT	168555	1.3478246	6.376599	1
TATGA	240270	1.3437777	6.0006394	4
TCATA	241375	1.3216531	6.0767226	2
GACTT	162705	1.3155322	6.814835	7
TGAGT	158695	1.3105891	6.5723486	8
GTGTT	153415	1.2530321	5.7802324	1
ATGAG	148740	1.2420526	5.260492	7
GATTG	147605	1.2190019	5.538989	7
GTTCT	149275	1.1936548	5.313832	1
ACATG	138030	1.1284516	5.4079485	8
TGGAC	92810	1.1204168	7.0969534	5
GTCCA	93800	1.1086258	8.060519	1
ATGTG	132495	1.0942154	5.066393	7
ACACT	135580	1.0851815	5.1973853	6
GGACT	87045	1.0508208	7.2828746	6
AGACT	123430	1.0090907	5.0668488	6
GTATG	121990	1.0074594	5.3556914	3
GAGTA	118160	0.9866944	5.046913	1
GTATA	139400	0.7796338	7.498278	1
>>END_MODULE