##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-130_GGACTCCT-CTCTCTAT_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1174790	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47878088849922	34.0	31.0	34.0	31.0	34.0
2	32.66257458779867	34.0	31.0	34.0	31.0	34.0
3	32.77093437976149	34.0	31.0	34.0	31.0	34.0
4	36.27392299900408	37.0	37.0	37.0	35.0	37.0
5	36.0006137267086	37.0	35.0	37.0	35.0	37.0
6	36.09329241821943	37.0	36.0	37.0	35.0	37.0
7	36.093380944679474	37.0	36.0	37.0	35.0	37.0
8	36.12007677967977	37.0	36.0	37.0	35.0	37.0
9	37.980427991385696	39.0	38.0	39.0	35.0	39.0
10-14	38.17022531686514	39.4	38.2	39.4	35.2	39.4
15-19	39.314316260778526	40.8	39.0	41.0	36.0	41.0
20-24	39.24204223733604	40.0	39.0	41.0	36.0	41.0
25-29	39.05170677312542	40.0	39.0	41.0	36.0	41.0
30-34	38.851662339652194	40.0	38.0	41.0	35.0	41.0
35-39	38.62125741621907	40.0	38.0	41.0	34.8	41.0
40-44	38.378459128865586	40.0	38.0	41.0	34.0	41.0
45-49	38.190869687348375	40.0	38.0	41.0	33.4	41.0
50-54	38.12489091667447	40.0	37.4	41.0	33.4	41.0
55-59	37.732555094953135	39.6	36.6	41.0	33.0	41.0
60-64	37.17436052400855	39.0	35.4	40.8	32.0	41.0
65-69	36.440060436333304	37.6	35.0	40.0	31.0	41.0
70-74	35.85053890482554	36.6	35.0	39.2	31.0	40.8
75-79	34.67038364303407	35.2	33.6	37.4	30.4	39.2
80-84	34.467882259808135	35.0	34.0	36.4	31.0	38.2
85-89	33.86721201235966	35.0	34.0	35.6	30.4	36.8
90-94	33.465350913780334	35.0	34.0	35.0	30.0	36.0
95-99	33.205180670587936	35.0	34.0	35.0	29.0	35.6
100-101	32.860824487780796	35.0	33.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	0.0
11	0.0
12	1.0
13	4.0
14	10.0
15	35.0
16	109.0
17	205.0
18	372.0
19	543.0
20	907.0
21	1259.0
22	1816.0
23	2525.0
24	3395.0
25	4327.0
26	5793.0
27	7509.0
28	9739.0
29	12042.0
30	15393.0
31	19336.0
32	25282.0
33	34280.0
34	49644.0
35	79797.0
36	147798.0
37	287332.0
38	384830.0
39	80437.0
40	69.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.21008861158165	23.43593323061994	10.906800364320432	24.447177793477984
2	15.56086788475651	23.46729937869586	39.557867780893666	21.41396495565397
3	15.928038202572376	27.289473012197924	32.95916717030278	23.823321614926922
4	11.555937656942943	18.606559470203184	43.51007414091029	26.32742873194358
5	10.701657317478018	39.17849147507214	38.35715319333668	11.76269801411316
6	25.521837945505155	43.632393874649935	19.393423505477575	11.452344674367334
7	23.938320891393357	33.83072719379634	26.553852177836042	15.677099736974268
8	21.275547119059578	38.266243328594896	24.24271571940517	16.215493832940357
9	22.84655129852995	15.01936516313554	25.190119085113082	36.943964453221426
10-14	20.533082508363197	25.895045071885185	32.59898364814137	20.972888771610247
15-19	19.878676280313908	31.615448889591057	28.524557566116528	19.98131726397851
20-24	19.761667797658184	29.922000741585407	30.21975851827286	20.096572942483547
25-29	19.85073551860221	30.495948275230884	29.624202974630553	20.02911323153635
30-34	19.622327469875987	30.18305441645722	29.7658283213196	20.42878979234719
35-39	19.853754925901566	30.528497419915883	29.561353340893	20.05639431328955
40-44	19.56369812813225	30.257243210291428	30.044178306828112	20.134880354748212
45-49	19.773221619062056	29.984516933433035	30.03120459620537	20.21105685129954
50-54	19.463238487808617	29.62970217767142	30.486105315153424	20.42095401936654
55-59	19.5981406635412	29.646838138503977	30.4682046005855	20.286816597369317
60-64	19.304769003521088	29.22193349176414	30.713694908048357	20.75960259666641
65-69	19.60776766493174	29.448473308444832	30.086723710385044	20.857035316238385
70-74	19.54285013836904	29.156685169784048	30.032287528399493	21.26817716344742
75-79	19.755534796998848	29.550860989229783	29.489589765407313	21.204014448364056
80-84	19.56941019005462	29.300539688587378	29.357418006950358	21.772632114407646
85-89	19.566524969325584	29.19867540310876	29.310068615017876	21.92473101254778
90-94	19.425860992297324	28.709844591341486	29.786846820220763	22.077447596140424
95-99	19.558953817699187	28.66321793603244	29.33618347891639	22.44164476735199
100-101	19.512235606664518	27.84211796421228	30.221780345994194	22.423866083129006
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.65726640505962
2	36.974832840410485
3	39.7513598174993
4	37.88336638888652
5	22.46435533159118
6	36.97418261987249
7	39.61542062836762
8	37.491040951999935
9	59.79051575175137
10-14	41.505971279973444
15-19	39.85999354429242
20-24	39.85824074014173
25-29	39.87984875013856
30-34	40.05111726222318
35-39	39.910149239191114
40-44	39.69857848288046
45-49	39.984278470361595
50-54	39.88419250717516
55-59	39.88495726091052
60-64	40.0643716001875
65-69	40.46480298117012
70-74	40.81102730181646
75-79	40.9595492453629
80-84	41.34204230446227
85-89	41.491255981873365
90-94	41.50330858843775
95-99	42.00059858505117
100-101	41.936101689793524
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	1.5
7	4.0
8	8.0
9	8.5
10	13.5
11	26.0
12	46.0
13	73.0
14	116.0
15	173.0
16	262.5
17	404.0
18	610.5
19	911.0
20	1322.5
21	1928.5
22	2802.5
23	3988.0
24	5481.0
25	7194.0
26	9421.5
27	12057.0
28	14943.0
29	18168.5
30	21591.0
31	25559.5
32	29882.5
33	34574.5
34	40331.5
35	47359.0
36	55680.0
37	63398.0
38	68404.5
39	69216.0
40	66018.0
41	61429.0
42	55840.0
43	50618.5
44	46991.5
45	43218.5
46	39577.5
47	36080.0
48	32241.0
49	28064.5
50	24212.5
51	21749.5
52	19466.5
53	17193.0
54	15137.0
55	13188.5
56	11716.5
57	10115.0
58	8387.0
59	6962.5
60	5779.0
61	4866.5
62	4035.5
63	3019.0
64	2232.5
65	1823.5
66	1431.5
67	1113.5
68	924.5
69	911.0
70	958.0
71	787.0
72	649.0
73	569.0
74	449.5
75	356.0
76	230.0
77	150.0
78	100.5
79	72.5
80	51.5
81	32.0
82	25.0
83	20.0
84	13.0
85	7.0
86	6.5
87	4.5
88	2.0
89	1.5
90	1.0
91	0.0
92	0.0
93	0.0
94	0.5
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.013959941776828197
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0013278969007226823
20-24	8.171673235216507E-4
25-29	0.017347781305595042
30-34	0.006741630419053618
35-39	0.04147124166872378
40-44	0.0505452038236621
45-49	0.05141344410490385
50-54	0.05107295772010317
55-59	0.040739195941402295
60-64	0.05171988185122447
65-69	0.04136909575328357
70-74	0.0028090126746056743
75-79	0.001140629389082304
80-84	1.1917023468024072E-4
85-89	0.0027749640361256055
90-94	0.002485550609045021
95-99	0.0016683832855233702
100-101	1.2768239430025792E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1174790.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	30.21929637070919
#Duplication Level	Relative count
1	100.0
2	7.560739533687009
3	3.330106960389125
4	2.266629093715924
5	1.5946041975520406
6	1.272987907227484
7	0.98200173979098
8	0.7743295066099806
9	0.6285301216628482
10++	4.110562491576716
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	164470	2.874041	6.7331614	95-97
GAGCC	158465	2.7691064	6.3462305	95-97
CTCCG	166950	2.7336836	6.0472026	95-97
CGAGC	150065	2.62232	6.2530284	95-97
GCCCA	150520	2.4977124	5.854324	90-94
AGCCC	149340	2.4781313	5.7046666	90-94
ACACA	301085	2.3735182	7.14374	6
CAAAG	283505	2.3535433	5.091725	4
ATCTC	293590	2.2535748	5.1373887	95-97
CCCAC	140995	2.2217433	5.5195513	90-94
CTTTG	276510	2.2055316	8.914281	9
TACAC	274645	2.1364307	8.859578	5
GACTC	184110	2.132288	5.2054844	7
CCACG	126170	2.093651	5.538918	90-94
GACGG	110230	2.0284493	5.978151	95-97
GCTTT	232560	1.8549725	5.2951694	1
CGGAC	105765	1.8481969	5.463453	90-94
ATACA	324515	1.7854944	5.332728	6
GGACT	144830	1.766384	6.9482813	6
GAGAG	132075	1.7190623	5.641849	7
TGGCT	137960	1.6603261	5.0299125	6
TTGAG	184705	1.572264	5.472721	9
GAGTC	124530	1.5187998	8.006784	9
TATAC	273900	1.487063	5.6491103	5
GTCTT	177085	1.4124862	6.416863	1
AAGAC	169800	1.4096106	5.647186	5
CCATG	121705	1.4095384	5.581717	9
TATGA	239530	1.369479	6.098613	4
TGAGT	158605	1.350093	6.240388	8
GACTT	164765	1.3318452	6.5843215	7
TCATA	243910	1.3242408	6.271235	2
GTGTT	155395	1.3052615	6.073123	1
ATGAG	148990	1.2852582	5.065127	7
GATTG	146670	1.2484988	6.0092626	7
GTATG	144965	1.2339853	5.4273214	3
GTGTA	144020	1.2259412	10.66216	1
AACAC	152725	1.2039642	5.1982274	5
GTTCT	149130	1.1895082	5.360923	1
ATGTG	133090	1.1329019	5.1673055	7
TGGAC	92685	1.1304101	6.9896746	5
ACATG	137045	1.1226344	5.361939	8
GTCCA	94775	1.0976459	7.3516035	1
ACACT	135335	1.0527548	5.4123006	6
TGTAT	181440	1.0236279	5.0365925	2
GAGTA	118575	1.0228839	5.270814	1
AGACT	124540	1.0201969	5.508896	6
CATAC	121695	0.94665086	5.076625	3
GGATA	103080	0.8892167	5.0198226	1
GTATA	137295	0.7849648	7.222326	1
>>END_MODULE