##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-128_TCCTGAGC-CTAAGCCT_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1163287	
Filtered Sequences	0	
Sequence length	101	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.551219088668574	31.0	31.0	34.0	30.0	34.0
2	31.74344508276977	33.0	31.0	34.0	30.0	34.0
3	31.88361341612173	34.0	31.0	34.0	30.0	34.0
4	35.416977925481845	37.0	35.0	37.0	33.0	37.0
5	35.286453815782345	37.0	35.0	37.0	32.0	37.0
6	35.436762381080506	37.0	35.0	37.0	33.0	37.0
7	35.45127556656268	37.0	35.0	37.0	33.0	37.0
8	35.498901818725734	37.0	35.0	37.0	33.0	37.0
9	37.26771897218829	39.0	37.0	39.0	34.0	39.0
10-14	37.3797590792298	39.2	37.2	39.4	33.2	39.4
15-19	38.413637047435415	40.0	38.0	41.0	33.6	41.0
20-24	38.30800258233781	40.0	38.0	41.0	33.6	41.0
25-29	38.12248997882723	40.0	38.0	41.0	33.0	41.0
30-34	37.85323621771756	40.0	38.0	41.0	32.4	41.0
35-39	37.53374068480091	40.0	37.2	41.0	31.6	41.0
40-44	37.488366671337346	40.0	37.0	41.0	31.6	41.0
45-49	37.323432996328506	40.0	37.0	41.0	31.0	41.0
50-54	36.65378982142842	38.8	35.8	40.2	30.6	40.6
55-59	37.30517748414622	40.0	36.4	41.0	31.0	41.0
60-64	36.85405200952129	39.0	35.4	41.0	31.0	41.0
65-69	36.174317085981365	38.0	35.0	40.0	29.6	41.0
70-74	35.3355923344798	36.6	34.4	39.2	29.0	41.0
75-79	34.38183646855849	35.4	34.0	37.6	28.6	39.4
80-84	33.46515932869533	35.0	33.2	36.4	27.2	38.2
85-89	32.73141348609586	35.0	33.0	35.4	26.0	36.6
90-94	32.18263437999393	35.0	32.2	35.0	25.2	36.0
95-99	31.754867543435115	34.8	32.0	35.0	24.4	35.2
100-101	30.94856858195785	34.0	30.0	35.0	22.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	3.0
6	30.0
7	121.0
8	224.0
9	318.0
10	403.0
11	543.0
12	893.0
13	1413.0
14	1676.0
15	2011.0
16	2326.0
17	2686.0
18	2939.0
19	3279.0
20	3984.0
21	4389.0
22	5057.0
23	5758.0
24	6463.0
25	7703.0
26	8917.0
27	10301.0
28	12595.0
29	15320.0
30	18254.0
31	23407.0
32	30290.0
33	40736.0
34	57990.0
35	88313.0
36	150568.0
37	272267.0
38	321589.0
39	60481.0
40	40.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.133487435353686	23.227783768723135	10.124210356137336	24.51451843978584
2	14.874350857912374	24.232144131686734	39.91827194700059	20.975233063400296
3	15.328563185823358	28.06642205400683	33.482841141361405	23.122173618808404
4	11.278159282782058	19.027669574818262	44.37072311679556	25.32344802560412
5	10.729690869877786	38.625270450159775	38.36625745135337	12.278781228609068
6	24.56235665482654	43.65111976865336	20.105116928398765	11.681406648121339
7	23.229594963890865	34.59608571976284	26.905927124963142	15.268392191383152
8	20.934740765766346	38.051309560345	25.299225533912228	15.714724139976427
9	22.889529031198773	15.616148435075559	25.61440337389956	35.879919159826116
10-14	20.301973150300544	26.179608946231824	33.19672356638836	20.32169433707927
15-19	19.625024247235125	31.876653455076237	28.84222375692508	19.65609854076356
20-24	19.431490698150704	30.138070819137425	30.638694255495242	19.79174422721663
25-29	19.50929495858639	31.044241853747856	29.741477651266045	19.704985536399704
30-34	19.251289732583583	30.601039626072975	29.959723687925116	20.18794695341833
35-39	19.548140874657786	30.988064439201953	29.640695243536573	19.823099442603688
40-44	19.23006755612382	30.750906545785874	30.188365848160437	19.83066004992987
45-49	19.526041611144905	30.33315807083791	30.261803653965124	19.878996664052057
50-54	19.295044919579407	30.11284741923038	30.648801307801765	19.943306353388447
55-59	19.47115562334289	30.164682305321882	30.704310301399758	19.659851769935468
60-64	19.26901182068217	29.678547184721626	30.967804167466195	20.084636827130005
65-69	19.66002873616582	29.89378004384019	30.526123568264214	19.920067651729774
70-74	19.65571917590918	29.616999749115585	30.484537076985063	20.24274399799017
75-79	19.724777898325836	30.218241652811283	29.951908776261472	20.10507167260141
80-84	19.82984639784972	30.023639035978515	29.839107370193357	20.307407195978406
85-89	19.926587811953656	29.939469677066157	29.86108149894565	20.27286101203454
90-94	19.950703553316078	29.44280571921315	30.387536785345524	20.21895394212525
95-99	20.331402986306458	29.455060970996648	29.701764823208183	20.51177121948871
100-101	20.320284177414877	28.76423745526415	30.845104567763364	20.070373799557608
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	66.64800587513953
2	35.84958392131267
3	38.45073680463177
4	36.60160730838618
5	23.008472098486855
6	36.243763302947876
7	38.49798715527402
8	36.649464905742775
9	58.769448191024885
10-14	40.62366748737982
15-19	39.28112278799868
20-24	39.223234925367336
25-29	39.2142804949861
30-34	39.43923668600191
35-39	39.371240317261474
40-44	39.06072760605369
45-49	39.405038275196965
50-54	39.23835127296786
55-59	39.13100739327836
60-64	39.35364864781217
65-69	39.580096387895594
70-74	39.89846317389936
75-79	39.829849570927244
80-84	40.13725359382813
85-89	40.19944882398819
90-94	40.16965749544133
95-99	40.843174205795165
100-101	40.390657976972484
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	1.5
2	2.5
3	4.0
4	6.5
5	7.5
6	10.5
7	18.5
8	32.0
9	41.5
10	40.5
11	54.5
12	84.0
13	135.0
14	192.5
15	267.5
16	388.0
17	548.5
18	785.0
19	1140.5
20	1645.0
21	2394.5
22	3409.0
23	4742.5
24	6392.5
25	8283.0
26	10706.0
27	13432.5
28	16389.0
29	19689.5
30	23236.5
31	27176.5
32	31139.0
33	35641.5
34	41384.5
35	48564.5
36	57656.5
37	66896.5
38	73406.5
39	74753.0
40	70666.0
41	64065.0
42	56449.0
43	49245.0
44	44240.5
45	39919.0
46	35993.0
47	32676.5
48	29095.0
49	24910.5
50	21211.5
51	18948.5
52	16874.5
53	14702.5
54	12727.5
55	10926.0
56	9540.5
57	8259.5
58	6762.0
59	5538.0
60	4452.0
61	3612.0
62	2918.0
63	2000.0
64	1343.5
65	1077.5
66	837.5
67	557.5
68	415.5
69	460.5
70	476.0
71	380.5
72	311.5
73	277.0
74	206.5
75	136.0
76	102.5
77	78.0
78	50.0
79	26.0
80	18.0
81	14.0
82	12.5
83	9.5
84	6.0
85	6.5
86	7.0
87	5.5
88	4.0
89	5.0
90	7.0
91	5.0
92	1.5
93	1.5
94	3.0
95	4.0
96	4.0
97	4.0
98	2.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03619055314810533
2	0.03490110351099944
3	0.03094679129054137
4	0.03000119488999705
5	0.0353309200567014
6	9.455964005443197E-4
7	6.877064731231416E-4
8	0.002922752510773352
9	3.438532365615708E-4
10-14	0.0061033949489678815
15-19	0.023020974187797163
20-24	0.018671230745293293
25-29	0.0018911928010886393
30-34	0.0019427707865728749
35-39	0.004917101282830462
40-44	0.01165662471943725
45-49	0.0068254867457471806
50-54	0.004521670060784656
55-59	0.0035416883365841792
60-64	0.00906053278339739
65-69	0.013186771622136238
70-74	0.01733020312270317
75-79	0.006963028040371809
80-84	0.010848569613517559
85-89	0.00871667954683582
90-94	0.010315597096847123
95-99	0.009541927314583589
100-101	0.006017431639827489
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1163287.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	26.31339827879953
#Duplication Level	Relative count
1	100.0
2	7.051388460717589
3	2.858142237188456
4	1.7797434134079537
5	1.2912328962033721
6	0.958642604757049
7	0.7386943015011027
8	0.5620242358130381
9	0.4659821195665062
10++	2.8646636154521095
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATATGGACTTTGGCTACACCATGAAAGCTTTGAGAAGCAAGAAGAAGGT	1225	0.10530505369698104	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CAAAG	323190	2.6940432	5.1191893	4
CTTTG	315110	2.546868	11.213835	9
ACACA	283150	2.3231459	7.2732115	6
GGCTT	176860	2.1861842	5.3547215	1
GCTTT	261770	2.1157494	6.241884	1
TACAC	251010	2.0383723	9.16269	5
GCCAA	162965	2.0239496	5.5788493	1
TTGGC	156210	1.9309275	5.1207767	5
TGGCT	155785	1.9256741	5.688706	6
CACAT	226890	1.8425013	5.1982865	7
GACTC	148985	1.8313881	5.758493	7
GAGTC	141085	1.7619982	10.265646	9
CACCA	143870	1.7586882	5.115914	7
GAGAG	133065	1.705857	5.2221813	7
TTGAG	203300	1.6866924	5.322925	9
AAGAC	194270	1.6193935	5.7419024	5
ATACA	290835	1.5851868	5.6140776	6
GTGTA	188720	1.5657282	10.480155	1
CATGA	188990	1.559258	5.0973043	9
CCATG	124635	1.5320675	5.6690516	9
GTCTT	188920	1.5269408	7.0887723	1
GACTT	186610	1.5238657	8.656768	7
TCATG	185965	1.5185986	5.506141	5
TGTGT	184330	1.5136554	6.949087	8
ATGGA	178190	1.4936513	5.2575717	4
TATGA	271730	1.489329	6.0060663	4
ACTCA	180875	1.4688282	5.2180667	8
TGAGT	176525	1.4645516	8.087156	8
TCATA	267140	1.4411371	6.107766	2
TCAAG	174550	1.4401212	5.0053887	8
TCCAT	179120	1.4396905	5.6494823	2
GTGTT	173815	1.4273098	6.242065	1
AACAC	172670	1.4166964	5.4247108	5
ATGAG	162980	1.3661557	6.443013	7
ACACC	109865	1.3430061	5.2877827	6
CAAGT	159810	1.3185091	5.7494717	9
GATTG	157605	1.3075807	5.9465737	7
TATAC	236960	1.2783253	5.2939897	5
GTTCT	152490	1.2324964	5.516539	1
GTCCA	100060	1.229981	9.165159	1
ACACT	151110	1.2271162	5.729881	6
TGGAC	98160	1.2259116	8.348234	5
ATGTG	143010	1.1864923	6.976562	7
GGACT	94720	1.1829498	9.145168	6
ACATG	143320	1.1824588	5.4375086	8
AGACT	137645	1.1356373	5.9294925	6
ACTTT	204745	1.0932316	5.429985	8
CCATA	134555	1.0926783	5.21149	3
GTATG	130710	1.0844445	5.022532	3
GTATA	152275	0.83460635	7.380755	1
>>END_MODULE