##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-128_TCCTGAGC-CTAAGCCT_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1163287	
Filtered Sequences	0	
Sequence length	101	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.449527072854764	34.0	31.0	34.0	31.0	34.0
2	32.62986434130185	34.0	31.0	34.0	31.0	34.0
3	32.73617774461504	34.0	31.0	34.0	31.0	34.0
4	36.24967183506735	37.0	37.0	37.0	35.0	37.0
5	35.97629819640381	37.0	35.0	37.0	35.0	37.0
6	36.07147419338478	37.0	36.0	37.0	35.0	37.0
7	36.07172950441293	37.0	36.0	37.0	35.0	37.0
8	36.09273979679993	37.0	36.0	37.0	35.0	37.0
9	37.94872976316249	39.0	38.0	39.0	35.0	39.0
10-14	38.12858047927983	39.2	38.2	39.4	35.2	39.4
15-19	39.260374438981955	40.0	39.0	41.0	36.0	41.0
20-24	39.187957228095904	40.0	39.0	41.0	36.0	41.0
25-29	39.00335600758884	40.0	38.8	41.0	35.8	41.0
30-34	38.81091287016875	40.0	38.0	41.0	35.0	41.0
35-39	38.58630621678055	40.0	38.0	41.0	34.6	41.0
40-44	38.35557175486359	40.0	38.0	41.0	34.0	41.0
45-49	38.17721095482027	40.0	38.0	41.0	33.4	41.0
50-54	38.13220744321908	40.0	37.6	41.0	33.4	41.0
55-59	37.76523695356347	39.8	36.6	41.0	33.0	41.0
60-64	37.21290893820699	39.0	35.6	40.6	32.0	41.0
65-69	36.472123732148646	37.8	35.0	40.0	31.0	41.0
70-74	35.891882742607805	36.6	35.0	39.2	31.0	40.8
75-79	34.728496235236875	35.2	33.6	37.4	30.4	39.2
80-84	34.533370182938526	35.0	34.0	36.6	31.0	38.4
85-89	33.9047889299889	35.0	34.0	35.8	30.4	36.8
90-94	33.48811411113508	35.0	34.0	35.0	30.0	36.0
95-99	33.22233653432042	35.0	34.0	35.0	29.2	35.6
100-101	32.8793264258949	35.0	33.5	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	6.0
14	7.0
15	33.0
16	97.0
17	163.0
18	324.0
19	521.0
20	771.0
21	1270.0
22	1678.0
23	2402.0
24	3330.0
25	4295.0
26	5580.0
27	7146.0
28	9242.0
29	11988.0
30	15042.0
31	19369.0
32	25291.0
33	34394.0
34	50063.0
35	79941.0
36	147091.0
37	286658.0
38	379057.0
39	77465.0
40	61.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.32531352967926	23.2568575080784	10.62111069753208	24.796718264710258
2	14.95711578737409	24.118930611486917	39.8068318987011	21.1171217024379
3	15.398693529627685	27.751105273247273	33.87005958116956	22.98014161595548
4	11.578054254882932	18.954135995674328	44.18367952190646	25.284130227536284
5	10.479529127377853	38.79274847909415	38.57276837100389	12.154954022524105
6	24.404037868556944	43.8826360132968	20.028849286547516	11.684476831598738
7	23.073583732991086	34.67759890723441	26.97949861040311	15.269318749371394
8	20.74079741284825	38.23329926320847	25.24149242620265	15.784410897740624
9	22.847930046497552	15.345912057815484	25.668128329466416	36.138029566220546
10-14	20.18172643552279	26.112111628514718	33.287176767212216	20.41898516875027
15-19	19.528438569769953	31.82715569257093	28.93657720722807	19.707828530431044
20-24	19.364306944923932	30.06643981354764	30.721381915111635	19.847871326416787
25-29	19.41997878738689	30.99682773762391	29.863252930834776	19.719940544154426
30-34	19.216843585014857	30.51801100883864	30.043015419045282	20.22212998710122
35-39	19.496780920441235	30.968919641089528	29.674270188026032	19.860029250443205
40-44	19.14344774889871	30.662199515292173	30.26838515642914	19.92596757937998
45-49	19.438016739326685	30.26019648479644	30.338773889732916	19.963012886143954
50-54	19.214019394929796	30.047399316231964	30.681368396155417	20.05721289268282
55-59	19.35500294206227	30.105888441745876	30.730661724070853	19.808446892121
60-64	19.118237238244042	29.605195821228165	30.992896101637506	20.283670838890284
65-69	19.501366754664616	29.80822089342341	30.478302210681807	20.21211014123017
70-74	19.37719106706578	29.574363462242726	30.461020282898026	20.587425187793468
75-79	19.397859275270324	30.182667887134677	29.90909416011559	20.51037867747941
80-84	19.363705697036053	29.93607072932047	29.72611369039555	20.974109883247934
85-89	19.340582783738753	29.921081547806434	29.730134001987878	21.008201666466938
90-94	19.206902551526024	29.32967165977452	30.33591550894007	21.127510279759377
95-99	19.39671654026337	29.377694150787164	29.78277957960932	21.442809729340144
100-101	19.18670972855366	28.590064188802934	30.978855604850736	21.244370477792668
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	66.12203179438953
2	36.07423748981199
3	38.37883514558316
4	36.86218448241922
5	22.63448314990196
6	36.08851470015568
7	38.342902482362476
8	36.52520831058887
9	58.9859596127181
10-14	40.600711604273066
15-19	39.236267100201
20-24	39.21217827134072
25-29	39.139919331541314
30-34	39.438973572116076
35-39	39.35681017088444
40-44	39.06941532827869
45-49	39.40102962547064
50-54	39.27123228761262
55-59	39.163449834183275
60-64	39.40190807713433
65-69	39.713476895894786
70-74	39.964616254859244
75-79	39.90823795274973
80-84	40.33781558028398
85-89	40.348784450205684
90-94	40.334412831285405
95-99	40.83952626960352
100-101	40.43108020634632
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	0.5
5	0.0
6	2.0
7	6.0
8	7.5
9	10.0
10	23.5
11	35.0
12	54.5
13	92.0
14	161.0
15	236.0
16	332.5
17	517.5
18	766.0
19	1136.0
20	1687.0
21	2473.0
22	3492.5
23	4887.5
24	6524.5
25	8295.5
26	10725.0
27	13517.0
28	16516.0
29	19807.5
30	23261.0
31	27040.0
32	30824.0
33	35319.5
34	41202.0
35	48316.0
36	57361.0
37	66618.5
38	73044.5
39	74278.0
40	70252.0
41	63708.5
42	55767.5
43	48585.5
44	44323.0
45	40451.0
46	36551.5
47	33458.0
48	29737.0
49	25308.5
50	21767.0
51	19400.0
52	17006.0
53	14791.5
54	12901.0
55	11131.0
56	9689.0
57	8281.0
58	6675.5
59	5509.0
60	4532.0
61	3613.0
62	2881.0
63	1976.0
64	1304.0
65	1027.5
66	774.5
67	519.0
68	370.0
69	421.0
70	469.5
71	387.5
72	326.0
73	254.5
74	195.5
75	158.5
76	98.0
77	63.5
78	28.5
79	8.0
80	5.5
81	3.0
82	3.0
83	2.0
84	1.5
85	0.5
86	0.0
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.013324312916760866
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	9.627890623723982E-4
20-24	7.392844586073772E-4
25-29	0.017656863697436662
30-34	0.006550404156497923
35-39	0.04308481054116482
40-44	0.05219692131004644
45-49	0.05259235253209225
50-54	0.052609545193920325
55-59	0.04274095730460325
60-64	0.05412249943479125
65-69	0.045784058448173154
70-74	0.0035244956747561007
75-79	0.001495761579042833
80-84	1.2034863279654977E-4
85-89	0.00306029380539798
90-94	0.0025960919360398594
95-99	0.002252238699478289
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1163287.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	27.176225415707897
#Duplication Level	Relative count
1	100.0
2	7.348496139744239
3	2.917101380153218
4	1.8511342295883388
5	1.2746296241094583
6	1.0021760515098221
7	0.7344919068769189
8	0.5997555668167049
9	0.4650192267564909
10++	3.042895042775807
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATATGGACTTTGGCTACACCATGAAAGCTTTGAGAAGCAAGAAGAAGGT	1252	0.10762606304377167	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AGAAG	345180	3.006438	4.5396066	5
CAAAG	326660	2.7263446	5.3333893	4
CTTTG	311700	2.5189376	10.457606	9
GACTC	195825	2.3762195	5.718575	7
ACACA	285520	2.2834918	7.3248377	6
GCTTT	261075	2.1098228	5.9329066	1
GCCAA	164635	2.0193365	5.5075502	1
TACAC	253175	2.0031605	9.41013	5
TGGCT	153705	1.925574	5.5273466	6
GGTTG	142955	1.8689315	5.2547455	7
CACAT	230910	1.8269962	5.0752544	7
GAGTC	140715	1.7818893	10.247071	9
GGCTT	139325	1.7454255	5.4855604	1
TTGAG	202330	1.7247685	5.6251197	9
GAGAG	128070	1.7107147	5.0321584	7
AAGAC	194830	1.6260751	5.875631	5
CATGA	192075	1.5859429	5.0081472	9
ATACA	294380	1.5848986	5.6644273	6
GTCTT	195235	1.5777503	6.681377	1
ATGGA	178440	1.5375553	5.167867	4
TGTGT	182050	1.5352994	6.7916474	8
TCATG	187740	1.5335766	5.1763945	5
CCATG	126240	1.5318474	5.9421415	9
AGACT	184260	1.5214154	5.8208213	6
TGAGT	178310	1.520009	8.5389385	8
GACTT	185485	1.5151564	7.9131107	7
TATGA	267065	1.4844444	5.9261866	4
TCAAG	177375	1.4645667	5.352433	8
TCATA	274255	1.4607629	6.0591836	2
GTGTT	171780	1.4486884	6.6616907	1
ACTCA	182645	1.4451159	5.3668036	8
GTGTA	168330	1.4349341	10.586223	1
ATGAG	166320	1.4331216	6.663499	7
TCCAT	181335	1.4194123	5.3822527	2
AACAC	174245	1.3935523	5.3394923	5
CAAGT	162500	1.3417453	6.109059	9
GATTG	155275	1.3236467	6.2450814	7
TATAC	241760	1.2876849	5.48766	5
ACACC	108560	1.2759526	5.379474	6
GTTCT	153860	1.2433869	5.4548244	1
GTCCA	101945	1.237042	8.779065	1
GTATG	145030	1.2363129	5.174614	3
TGGAC	97500	1.234653	8.128892	5
ACATG	147380	1.2169011	5.4565187	8
ATGTG	142175	1.2119753	6.9229064	7
ACACT	152900	1.2097688	5.493397	6
GGACT	94985	1.2028054	8.853372	6
GTATA	152105	0.84545493	7.2395897	1
>>END_MODULE