##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-127_TCCTGAGC-AAGGAGTA_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	905197	
Filtered Sequences	0	
Sequence length	101	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.01762157850722	33.0	31.0	34.0	30.0	34.0
2	32.23392145577151	34.0	31.0	34.0	31.0	34.0
3	32.38005649598927	34.0	31.0	34.0	31.0	34.0
4	35.904584305957705	37.0	35.0	37.0	35.0	37.0
5	35.799596110018044	37.0	35.0	37.0	35.0	37.0
6	35.94601617106552	37.0	36.0	37.0	35.0	37.0
7	35.972424787090546	37.0	36.0	37.0	35.0	37.0
8	36.017033861137406	37.0	36.0	37.0	35.0	37.0
9	37.83986248297332	39.0	38.0	39.0	35.0	39.0
10-14	37.99713874438382	39.2	38.2	39.4	35.2	39.4
15-19	39.11728540859062	40.0	39.0	41.0	36.0	41.0
20-24	39.004082647202765	40.0	39.0	41.0	36.0	41.0
25-29	38.838676884700234	40.0	38.4	41.0	35.6	41.0
30-34	38.58689500738514	40.0	38.0	41.0	34.8	41.0
35-39	38.29147577820077	40.0	38.0	41.0	34.0	41.0
40-44	38.25367096886092	40.0	38.0	41.0	33.8	41.0
45-49	38.095364434482214	40.0	38.0	41.0	33.4	41.0
50-54	37.45109738543102	39.2	36.8	40.2	32.8	40.6
55-59	38.094015556834584	40.0	37.4	41.0	34.0	41.0
60-64	37.63813976405136	39.4	36.2	41.0	33.0	41.0
65-69	36.95706105963674	38.6	35.2	40.4	32.4	41.0
70-74	36.100758177501696	36.8	35.0	39.4	31.6	41.0
75-79	35.13481065447632	35.6	34.4	38.0	31.0	39.4
80-84	34.23220293483076	35.0	34.0	36.6	30.0	38.2
85-89	33.52184861416907	35.0	34.0	35.6	29.2	36.8
90-94	33.00557513999715	35.0	33.4	35.0	29.0	36.0
95-99	32.60797417578715	35.0	33.0	35.0	27.6	35.2
100-101	31.800451172507202	34.5	32.0	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	2.0
6	20.0
7	99.0
8	201.0
9	271.0
10	285.0
11	343.0
12	437.0
13	518.0
14	561.0
15	613.0
16	702.0
17	834.0
18	962.0
19	1107.0
20	1267.0
21	1479.0
22	1780.0
23	2154.0
24	2533.0
25	2892.0
26	3534.0
27	4378.0
28	5318.0
29	6853.0
30	9110.0
31	12269.0
32	17232.0
33	25332.0
34	39173.0
35	67634.0
36	128147.0
37	246246.0
38	276978.0
39	43906.0
40	27.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.097815949184685	23.608943955514498	10.59448086248127	24.69875923281955
2	14.878865967989322	23.54621481013274	40.39370217957275	21.181217042305192
3	15.29101324234013	27.62747299375895	32.94661957886176	24.134894185039162
4	11.057985555545734	18.807183331418013	44.18884753763602	25.945983575400227
5	10.475832651888522	39.202930581032994	38.69118394589697	11.630052821181515
6	24.911924471022072	43.91348105202339	19.66926244132441	11.505332035630127
7	23.79989681758476	33.82770304233462	26.74181086243486	15.630589277645763
8	20.721521924058464	38.52116177071711	24.78970801064993	15.9676082945745
9	22.676796355256442	14.98385981347645	25.131408294796476	37.207935536470636
10-14	20.15347986764405	26.076662597292117	33.0191407911527	20.75071674391113
15-19	19.456235564365223	31.82742677233848	28.81953276887713	19.89680489441917
20-24	19.353695959931244	30.01790930519233	30.647254311691746	19.98114042318468
25-29	19.377573108711932	30.779975770548912	29.94758829023285	19.89486283050631
30-34	19.122024467112926	30.41174905742754	30.050495900052855	20.41573057540668
35-39	19.408892247455665	30.889931812256116	29.775130696858415	19.926045243429805
40-44	19.051152553871106	30.580889802746064	30.30117879473238	20.066778848650447
45-49	19.343334239292556	30.364394476742255	30.16791180818413	20.12435947578106
50-54	19.16269277832796	29.89327397579298	30.676628233631494	20.267405012247576
55-59	19.408873329809857	29.97159417318186	30.764928967756646	19.854603529251637
60-64	19.04164444077143	29.47482953272366	31.167459433229727	20.316066593275178
65-69	19.496357934703802	29.714316146250084	30.585282754777175	20.204043164268935
70-74	19.329062221209607	29.27744159337778	30.82597203306248	20.56752415235013
75-79	19.441521601076882	29.836881034210744	30.31298901771987	20.4086083469925
80-84	19.2909102158426	29.609879085718326	30.272852622461066	20.82635807597801
85-89	19.369688275725526	29.559050928473162	30.1688482548841	20.902412540917208
90-94	19.24017639196368	29.03977657783946	30.812534815802444	20.907512214394412
95-99	19.605750036569688	28.9913660181642	30.211202654406943	21.19168129085917
100-101	19.546913874858035	28.144900060542582	31.33189268495318	20.9762933796462
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.79657518200423
2	36.06008301029451
3	39.42590742737929
4	37.00396913094596
5	22.10588547307004
6	36.4172565066522
7	39.43048609523052
8	36.68913021863297
9	59.88473189172707
10-14	40.904196611555186
15-19	39.35304045878439
20-24	39.33483638311593
25-29	39.272435939218234
30-34	39.537755042519606
35-39	39.334937490885466
40-44	39.117931402521556
45-49	39.467693715073615
50-54	39.43009779057553
55-59	39.2634768590615
60-64	39.35771103404661
65-69	39.70040109897274
70-74	39.89658637355974
75-79	39.85012994806939
80-84	40.11726829182061
85-89	40.272100816642734
90-94	40.14768860635809
95-99	40.79743132742885
100-101	40.523207254504236
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	2.5
2	4.0
3	3.0
4	7.0
5	12.5
6	16.5
7	21.0
8	22.5
9	25.5
10	32.5
11	41.5
12	62.5
13	86.0
14	122.5
15	149.0
16	217.0
17	353.0
18	489.5
19	659.0
20	995.0
21	1553.0
22	2254.5
23	3173.0
24	4354.5
25	5758.0
26	7403.0
27	9514.0
28	12122.5
29	14753.5
30	17797.5
31	21173.5
32	24481.0
33	28248.5
34	32845.5
35	38940.5
36	46465.0
37	53292.5
38	57870.0
39	58560.0
40	55392.5
41	50707.5
42	44749.0
43	38995.0
44	34923.5
45	31320.5
46	28010.5
47	24930.0
48	21748.5
49	18539.0
50	15843.5
51	14108.0
52	12644.0
53	11253.5
54	9711.0
55	8311.0
56	7302.0
57	6281.0
58	5371.0
59	4415.5
60	3528.5
61	2946.0
62	2401.0
63	1724.5
64	1178.0
65	933.5
66	708.5
67	513.0
68	393.0
69	404.5
70	424.5
71	354.5
72	302.5
73	246.5
74	192.0
75	136.5
76	93.5
77	72.0
78	48.5
79	31.5
80	23.5
81	15.0
82	10.5
83	9.5
84	11.5
85	7.5
86	3.5
87	4.0
88	4.0
89	6.5
90	5.0
91	3.5
92	3.0
93	1.0
94	2.5
95	2.5
96	3.0
97	4.5
98	3.0
99	1.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03015918081920289
2	0.02750782426366857
3	0.02441457494887853
4	0.023530789430367093
5	0.028391609782180013
6	8.837855185114402E-4
7	4.418927592557201E-4
8	0.00298277612497611
9	3.3141956944179E-4
10-14	0.00684933776846366
15-19	0.02558559076090619
20-24	0.021277136358162918
25-29	0.0019443281407251682
30-34	0.001612908571283378
35-39	0.00541318630088257
40-44	0.0132567827776716
45-49	0.00872738199530047
50-54	0.004220075850892126
55-59	0.003380479608306258
60-64	0.010671710136025638
65-69	0.014759218159141049
70-74	0.018913010096144815
75-79	0.007313325165682167
80-84	0.01027400665269549
85-89	0.00846224633974704
90-94	0.008440151701784252
95-99	0.008373867787895894
100-101	0.00541318630088257
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	905197.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	31.461110392131484
#Duplication Level	Relative count
1	100.0
2	7.638144430367411
3	3.616850209531235
4	2.3846360003898255
5	1.6774680830328428
6	1.2340415164214014
7	0.9002533866094923
8	0.6986404833836858
9	0.565856154370919
10++	3.104595068706754
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CTTTG	237040	2.4159472	10.271214	9
ACACA	223695	2.336601	8.459471	6
GCTGC	98585	2.3360736	5.472522	90-94
TACAC	200955	2.0506806	10.622708	5
GCTTT	201200	2.0506606	5.833932	1
GCCGA	81845	1.9851667	5.371099	90-94
GACGC	80520	1.9530288	5.3879247	95-97
CACAT	189120	1.929908	6.3642983	7
CGCTG	81405	1.9289756	5.2292356	95-97
CTGCC	85165	1.9284655	5.1768155	90-94
GCCAA	120730	1.9216831	6.0456347	1
CGACG	77920	1.889965	5.615362	95-97
TGGCT	115465	1.8356235	5.843874	6
TTGGC	115225	1.8318082	5.236118	5
CCGAC	76750	1.7789274	5.1486874	95-97
GAGTC	107385	1.7474551	10.401314	9
GACTC	112360	1.7472264	5.927484	7
TGCCG	73455	1.7405922	5.0840893	90-94
GGCTT	108690	1.7279168	5.390437	1
ATACA	238430	1.6708858	5.607486	6
TGTGT	153660	1.6388968	5.2863913	8
GAGAG	93665	1.632653	5.5200133	7
TTGAG	148910	1.6257129	6.3746576	9
CACCA	103530	1.5747364	5.1561933	7
GTGTA	141950	1.5497277	11.862646	1
CATGA	144030	1.5380735	6.188743	9
ATGGA	136240	1.5224875	5.350232	4
GTCTT	148085	1.5093045	6.96611	1
AAGAC	136970	1.4971962	5.890792	5
TATGA	204930	1.4682068	6.8845015	4
TGAGT	134425	1.4675741	7.6031227	8
CCATG	93730	1.457525	6.2442193	9
TCCAT	145935	1.4548877	5.8222833	2
TCATA	211360	1.4470366	7.6048207	2
ATGAG	126720	1.4161011	6.6280527	7
GACTT	134025	1.3982371	7.7258177	7
ACTCA	135105	1.3787025	5.39935	8
AACAC	131095	1.3693498	5.249216	5
TATAC	195960	1.3416033	5.8282776	5
GTGTT	123940	1.3219111	5.556594	1
GATTG	119670	1.3064874	7.089423	7
GTTCT	122640	1.2499653	5.171435	1
GTCCA	80060	1.2449532	9.4515295	1
ACACC	79115	1.2033736	5.731404	6
ACATG	111950	1.1954963	6.831035	8
TGGAC	73440	1.1950748	8.272372	5
ACACT	116315	1.1869568	5.498087	6
ACCAT	115130	1.1748642	5.1568494	8
GGACT	70700	1.1504874	8.428147	6
ATGTG	104875	1.1449642	5.606945	7
CCATA	108470	1.106901	5.2319803	3
GTATG	100655	1.0988927	6.201101	3
AGACT	99000	1.0572053	6.0176744	6
TGTAT	149630	1.0473002	5.4728594	2
GAGTA	93305	1.0426872	5.2798505	1
CATAC	101060	1.0312845	6.434792	3
GTATA	110645	0.7927084	7.107037	1
>>END_MODULE