##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-127_TCCTGAGC-AAGGAGTA_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	905197	
Filtered Sequences	0	
Sequence length	101	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.73117012097919	31.0	31.0	34.0	30.0	34.0
2	31.932539546640125	31.0	31.0	34.0	30.0	34.0
3	32.0121730407856	33.0	31.0	34.0	30.0	34.0
4	35.70804476815544	37.0	35.0	37.0	33.0	37.0
5	35.34762819585129	37.0	35.0	37.0	33.0	37.0
6	35.53796355931361	37.0	35.0	37.0	33.0	37.0
7	35.537520561822454	37.0	35.0	37.0	33.0	37.0
8	35.55749080034512	37.0	35.0	37.0	33.0	37.0
9	37.24754721900316	39.0	37.0	39.0	34.0	39.0
10-14	37.314285398648025	39.2	37.2	39.4	33.2	39.4
15-19	38.267844016274914	40.0	38.0	41.0	33.4	41.0
20-24	38.15360921434782	40.0	38.0	41.0	33.6	41.0
25-29	37.881608975725726	40.0	37.0	41.0	33.0	41.0
30-34	37.600326779695465	39.4	37.0	40.4	32.2	41.0
35-39	37.289523054097614	39.0	36.6	40.0	31.2	41.0
40-44	36.97569589824094	39.0	36.0	40.0	30.8	41.0
45-49	36.758804326572005	38.6	35.6	40.0	30.4	41.0
50-54	36.71284814244855	38.8	35.4	40.0	30.4	41.0
55-59	36.2879810693142	38.0	34.8	40.0	29.6	41.0
60-64	35.678776001246135	37.8	34.0	40.0	28.6	41.0
65-69	34.89600473709038	36.4	33.4	39.0	28.0	40.4
70-74	34.39592044604656	35.6	33.0	38.6	27.8	40.0
75-79	33.465385104016036	34.6	32.4	36.6	27.4	39.0
80-84	33.50734392623926	35.0	33.0	36.0	29.0	37.6
85-89	32.89107542336088	35.0	32.8	35.2	27.4	36.4
90-94	32.43486246640234	34.0	32.0	35.0	26.4	35.8
95-99	32.11765041200976	34.0	32.0	35.0	25.4	35.0
100-101	31.6835180629189	34.0	31.5	35.0	25.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	5.0
14	22.0
15	58.0
16	128.0
17	271.0
18	450.0
19	721.0
20	1032.0
21	1599.0
22	2438.0
23	3279.0
24	4258.0
25	5639.0
26	7567.0
27	9742.0
28	12572.0
29	16073.0
30	20919.0
31	26636.0
32	35211.0
33	47919.0
34	68093.0
35	103773.0
36	163175.0
37	224451.0
38	138437.0
39	10725.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.086633075452085	23.95047707847021	11.929557875247045	24.03333197083066
2	15.9218617765276	23.844620677616298	39.352280486214994	20.881237059641105
3	16.395436573475166	26.980425255496872	33.72912194804004	22.895016222987923
4	12.125979206736213	19.089656726657292	43.35674996713423	25.42761409947227
5	10.305270565412833	39.78437842812117	38.69058337577345	11.219767630692544
6	25.12767938912745	44.5635590926616	19.240894523512562	11.067866994698392
7	23.7989078620455	34.20426713742975	26.65994253184666	15.33688246867809
8	21.106344806710585	39.01879922271064	24.091882761432043	15.78297320914674
9	22.835250227298587	14.996404097671556	25.212522798904548	36.9558228761253
10-14	20.373421476209046	26.213365709342828	32.95156744885367	20.46164536559445
15-19	19.646875644203053	32.16221775758898	28.62081056734942	19.570096030858554
20-24	19.596907528327144	30.25548839483522	30.47195080644787	19.675653270389766
25-29	19.652830982550945	31.1036718346533	29.689534081243586	19.553963101552164
30-34	19.374166158093995	30.632727745176584	29.9499440754193	20.043162021310117
35-39	19.727152286263323	30.997073126741437	29.639553856280397	19.63622073071484
40-44	19.379597347718867	30.751022811775226	30.171819999274945	19.697559841230962
45-49	19.726631147682312	30.48695139328234	30.05861472424155	19.727802734793794
50-54	19.454519571191604	30.07178490965102	30.63569235720829	19.838003161949093
55-59	19.669641825004458	30.083653810661282	30.639106095364948	19.607598268969316
60-64	19.232465375063416	29.643322534973095	31.07955970984795	20.044652380115544
65-69	19.600309970192114	29.923108649149725	30.41567038126953	20.060910999388636
70-74	19.436902539736412	29.67850716245263	30.439716612930596	20.444873684880367
75-79	19.576912352366968	30.071910273230756	29.981232536692005	20.36994483771027
80-84	19.492379438791033	29.841168065516875	29.71944854361896	20.947003952073132
85-89	19.534672530764972	29.929151351847004	29.55264707474516	20.983529042642864
90-94	19.290930953065903	29.23015158605689	30.272822201570616	21.206095259306593
95-99	19.594316614573827	29.33117744251191	29.57389192057967	21.500614022334595
100-101	19.2772062625111	28.41572432931652	30.86707188277456	21.439997525397814
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	64.11996504628274
2	36.8030988361687
3	39.290452796463086
4	37.553593306208484
5	21.525038196105378
6	36.19554638382584
7	39.135790330723594
8	36.88931801585732
9	59.7910731034239
10-14	40.835066841803496
15-19	39.21697167506161
20-24	39.27256079871691
25-29	39.20679408410311
30-34	39.41732817940411
35-39	39.363373016978166
40-44	39.077157188949826
45-49	39.45443388247611
50-54	39.29252273314069
55-59	39.277240093973774
60-64	39.277117755178956
65-69	39.661220969580754
70-74	39.88177622461678
75-79	39.94685719007724
80-84	40.439383390864165
85-89	40.518201573407836
90-94	40.497026212372496
95-99	41.09493063690842
100-101	40.717203787908915
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	2.5
7	2.5
8	4.0
9	10.0
10	15.0
11	18.0
12	32.0
13	62.5
14	93.5
15	122.5
16	195.0
17	322.0
18	483.5
19	725.0
20	1061.5
21	1535.5
22	2255.5
23	3253.0
24	4431.5
25	5854.5
26	7730.0
27	9854.0
28	12223.5
29	14934.5
30	17928.0
31	21046.0
32	24365.0
33	28341.0
34	33178.0
35	39241.5
36	46296.5
37	52838.5
38	57162.0
39	57792.5
40	54719.5
41	49697.5
42	43733.5
43	38244.0
44	34686.5
45	31590.0
46	28469.0
47	25579.5
48	22571.5
49	19224.5
50	16302.5
51	14550.5
52	12911.5
53	11431.5
54	9939.5
55	8482.0
56	7434.0
57	6414.5
58	5368.5
59	4308.0
60	3435.0
61	2871.0
62	2333.5
63	1676.5
64	1147.5
65	888.5
66	699.0
67	500.5
68	376.0
69	388.5
70	426.5
71	347.5
72	284.5
73	237.5
74	168.5
75	131.0
76	89.0
77	48.5
78	22.5
79	15.0
80	10.5
81	7.0
82	6.0
83	6.0
84	3.5
85	3.0
86	2.0
87	0.0
88	1.0
89	1.0
90	0.0
91	0.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.00916927475455619
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	9.721640703625841E-4
20-24	5.744605870324361E-4
25-29	0.016659357023940644
30-34	0.0054573755768081425
35-39	0.03762716845062456
40-44	0.04818840539683627
45-49	0.048762865983868704
50-54	0.0484535410523897
55-59	0.03853304860709879
60-64	0.047503471619989905
65-69	0.0370968971395177
70-74	0.003557236712008546
75-79	0.001480340743506662
80-84	1.32567827776716E-4
85-89	0.0030932493147900397
90-94	0.002364126262018102
95-99	0.002010612054613526
100-101	1.1047318981393002E-4
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	905197.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	30.74064576878939
#Duplication Level	Relative count
1	100.0
2	7.5243234057016615
3	3.526969695130463
4	2.322179668485485
5	1.6126584890658477
6	1.2114664449286534
7	0.9177040404469559
8	0.6688557226008898
9	0.5492871406114386
10++	3.0559787325730308
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATATGGACTTTGGCTACACCATGAAAGCTTTGAGAAGCAAGAAGAAGGT	1017	0.11235123404076681	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AGAAG	277840	3.0405533	4.945373	5
CAAAG	246570	2.619543	5.661198	4
CTTTG	234015	2.451995	10.303129	9
CCGAG	101125	2.4227962	5.82413	95-97
CTCCG	104320	2.4151845	5.736536	95-97
GAGCC	99645	2.387338	5.615019	95-97
GACTC	148690	2.3265598	6.4184194	7
ACACA	219070	2.2594135	8.226291	6
CGAGC	92210	2.2092066	5.448504	95-97
AAAGC	204400	2.1715317	5.29546	5
AGCCC	92150	2.1432915	5.2646832	90-94
GCCCA	90855	2.113171	5.3932548	90-94
TACAC	198400	2.036814	10.72212	5
GCTTT	194280	2.035654	5.4670954	1
GCCAA	121805	1.9146998	5.5263968	1
CACAT	180365	1.851663	6.0927925	7
TGGCT	114220	1.8324999	5.2586446	6
TTGAG	160730	1.7428035	6.3562813	9
GAGTC	105435	1.6993771	8.088549	9
GAGAG	97675	1.6291611	5.596442	7
CACCA	106735	1.6288083	5.682633	7
ATACA	233730	1.6217126	5.493776	6
GGCTT	100660	1.6149486	5.687058	1
AAGAC	149530	1.5885966	6.243286	5
ATGGA	143675	1.5650777	5.3134675	4
GACTT	145545	1.5320619	8.370439	7
CATGA	143830	1.5210086	5.9274154	9
AGACT	143415	1.5166199	6.070986	6
CCATG	96735	1.5136173	6.4411798	9
TATGA	209360	1.4894383	7.1956234	4
TGAGT	134040	1.4534026	6.1249533	8
GTCTT	138260	1.4486799	5.406124	1
TGTGT	133980	1.446067	5.594373	8
TCATA	206320	1.4249433	6.9794703	2
GTGTT	131015	1.4140652	6.0136333	1
ACTCA	135270	1.3887088	5.7941265	8
GTGTA	124765	1.3528333	12.040805	1
ATGAG	123750	1.3480309	5.044096	7
GATTG	123595	1.3401469	7.102843	7
TATAC	190455	1.3153722	6.1348944	5
AACAC	124370	1.28271	5.270827	5
ACACC	83475	1.2738537	6.1561856	6
TGGAC	78405	1.2637137	8.54182	5
GTATG	114515	1.2416921	6.8767715	3
ACCAT	119385	1.2256302	5.371016	8
GGACT	75765	1.2211628	8.854421	6
ATGTG	110770	1.2010849	5.6465235	7
ATTGA	166225	1.1825653	5.1500797	8
GTCCA	75030	1.1739981	7.071586	1
ACATG	107650	1.1384034	6.2350664	8
ACACT	108590	1.1148065	5.385949	6
TGTAT	153860	1.0895606	5.9407363	2
GAGTA	98295	1.0707451	5.292866	1
ACTTT	154785	1.064099	5.0066633	8
CATAC	96870	0.99448675	6.003193	3
GTATA	114520	0.8147233	6.647816	1
ATACT	114240	0.7889954	5.0499015	6
>>END_MODULE