##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-126_TCCTGAGC-ACTGCATA_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1173561	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.537959253928854	31.0	31.0	34.0	30.0	34.0
2	31.733937988736844	33.0	31.0	34.0	30.0	34.0
3	31.878808174436607	34.0	31.0	34.0	30.0	34.0
4	35.408106608859704	37.0	35.0	37.0	33.0	37.0
5	35.27854879294728	37.0	35.0	37.0	32.0	37.0
6	35.424600851596125	37.0	35.0	37.0	33.0	37.0
7	35.431627329129036	37.0	35.0	37.0	33.0	37.0
8	35.48253137246381	37.0	35.0	37.0	33.0	37.0
9	37.25882847163462	39.0	37.0	39.0	34.0	39.0
10-14	37.37569346629617	39.2	37.2	39.4	33.2	39.4
15-19	38.40500340416902	40.0	38.0	41.0	33.6	41.0
20-24	38.292478533284594	40.0	38.0	41.0	33.6	41.0
25-29	38.10508239452402	40.0	38.0	41.0	33.0	41.0
30-34	37.83168987381141	40.0	38.0	41.0	32.4	41.0
35-39	37.509340715991755	40.0	37.0	41.0	31.4	41.0
40-44	37.45920663689403	40.0	37.0	41.0	31.4	41.0
45-49	37.28462022851816	40.0	36.8	41.0	31.0	41.0
50-54	36.60492944124762	38.8	35.4	40.2	30.2	40.6
55-59	37.238545077759056	39.8	36.0	41.0	31.0	41.0
60-64	36.78515833433456	39.0	35.2	41.0	30.8	41.0
65-69	36.11920471113133	37.8	35.0	40.0	29.6	41.0
70-74	35.29833898706586	36.6	34.2	39.2	29.0	41.0
75-79	34.36392518156278	35.4	34.0	37.6	28.6	39.4
80-84	33.47869399204643	35.0	33.4	36.4	27.4	38.2
85-89	32.74205925384364	35.0	33.0	35.4	26.0	36.6
90-94	32.2035321555505	35.0	32.4	35.0	25.2	36.0
95-99	31.755462050971357	34.8	32.0	35.0	24.4	35.2
100-101	30.910189585373065	34.0	30.0	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	4.0
6	27.0
7	108.0
8	251.0
9	306.0
10	424.0
11	622.0
12	984.0
13	1425.0
14	1779.0
15	2100.0
16	2458.0
17	2762.0
18	3105.0
19	3559.0
20	4096.0
21	4575.0
22	5096.0
23	5909.0
24	6850.0
25	7765.0
26	9132.0
27	10580.0
28	12593.0
29	15427.0
30	18808.0
31	23506.0
32	30374.0
33	41015.0
34	57861.0
35	89172.0
36	152047.0
37	272587.0
38	322298.0
39	63910.0
40	46.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.58653169952051	23.658177943526905	10.527096430474161	24.228193926478422
2	15.184379190097113	23.46837965721436	40.12775827193896	21.21948288074957
3	15.681701754789723	27.825269415243408	32.743828999419534	23.749199830547337
4	11.085805463974117	18.598769872793664	44.26582748897898	26.049597174253247
5	10.732694873139067	39.25959075468723	38.57501481074216	11.43269956143155
6	25.41319567529245	43.77956875630573	19.59496768739945	11.212267881002372
7	23.99474075734117	33.87950948955863	26.67932338803616	15.44642636506404
8	21.169351746281706	38.475034980170804	24.264052996023935	16.091560277523556
9	22.70728292629306	15.119129685663962	25.557771996663124	36.615815391379854
10-14	20.36912715646719	26.074217768013103	32.83570311870197	20.72095195681774
15-19	19.684250271250207	31.84737370991016	28.692825442715282	19.775550576124356
20-24	19.602587393920633	30.121665060397866	30.47055550763833	19.805192038043174
25-29	19.589155140929144	30.715523125468135	29.881102619184972	19.814219114417746
30-34	19.479471034999325	30.4129822919942	29.980136970542898	20.127409702463577
35-39	19.676664343686532	30.730590035250327	29.802369439739117	19.790376181324024
40-44	19.336615513628264	30.471711861272343	30.298012539553625	19.89366008554577
45-49	19.58999247866185	30.31740115184019	30.207146140469554	19.88546022902841
50-54	19.33528157699684	29.90043301183961	30.657176889605758	20.10710852155779
55-59	19.562800273367	29.934470317354545	30.67792970514199	19.824799704136463
60-64	19.196423625628093	29.522660921251486	30.999079454135565	20.28183599898486
65-69	19.57730423343461	29.721873092070478	30.441632313282412	20.259190361212497
70-74	19.505229835253882	29.461143902457316	30.535661810577757	20.49796445171104
75-79	19.736368027384103	29.744913628751394	30.18986778690059	20.328850556963914
80-84	19.74133005906768	29.629115784651127	30.067896470665232	20.561657685615955
85-89	19.906474502567377	29.53017217356535	29.956059684927094	20.607293638940178
90-94	19.921434789989412	29.079115384641607	30.433724409550813	20.565725415818168
95-99	20.293990270430758	28.992415380989005	29.92192790206142	20.791666446518814
100-101	20.433640339365514	28.226545909445722	30.715203615899323	20.624610135289444
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.81472562599893
2	36.403862070846685
3	39.43090158533706
4	37.13540263822736
5	22.165394434570615
6	36.625463556294825
7	39.441167122405204
8	37.26091202380527
9	59.323098317672915
10-14	41.09007911328493
15-19	39.45980084737456
20-24	39.40777943196381
25-29	39.40337425534689
30-34	39.6068807374629
35-39	39.46704052501055
40-44	39.23027559917403
45-49	39.47545270769026
50-54	39.44239009855463
55-59	39.38759997750346
60-64	39.47825962461295
65-69	39.83649459464711
70-74	40.00319428696492
75-79	40.06521858434802
80-84	40.30298774468364
85-89	40.51376814150755
90-94	40.487160205807584
95-99	41.08565671694957
100-101	41.05825047465496
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.5
3	2.5
4	6.5
5	13.0
6	21.5
7	25.5
8	24.5
9	32.5
10	47.5
11	59.5
12	91.5
13	134.0
14	190.5
15	253.5
16	360.0
17	565.5
18	812.5
19	1163.0
20	1689.5
21	2458.5
22	3528.0
23	4965.0
24	6570.5
25	8510.0
26	10883.5
27	13512.5
28	16831.0
29	20303.5
30	23947.0
31	27971.5
32	31985.0
33	36613.5
34	42531.0
35	49145.0
36	56948.0
37	64402.0
38	68792.0
39	69422.5
40	66281.5
41	61609.5
42	55996.0
43	50356.5
44	46059.0
45	42009.5
46	37871.0
47	33983.5
48	29992.5
49	25784.0
50	22195.5
51	19770.0
52	17605.0
53	15518.5
54	13565.0
55	11846.5
56	10631.0
57	9011.5
58	7274.5
59	6178.0
60	5156.0
61	4233.0
62	3447.0
63	2406.0
64	1679.0
65	1416.0
66	1155.0
67	862.0
68	702.5
69	710.5
70	703.0
71	597.5
72	524.5
73	419.5
74	300.0
75	226.5
76	161.5
77	125.0
78	91.5
79	54.0
80	41.5
81	38.5
82	26.5
83	22.0
84	18.5
85	12.0
86	8.0
87	8.5
88	9.5
89	7.5
90	6.5
91	5.0
92	5.0
93	6.0
94	5.0
95	3.0
96	3.5
97	6.5
98	5.0
99	1.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03715188217740705
2	0.03476598148711486
3	0.03169839488531061
4	0.029738547889713446
5	0.036299774788016985
6	0.002130268473475175
7	6.816859115120561E-4
8	0.003323218818621273
9	6.816859115120561E-4
10-14	0.006748690523969355
15-19	0.024711114292312035
20-24	0.02056987237987629
25-29	0.0023859006902921965
30-34	0.0020450577345361683
35-39	0.005828414543428079
40-44	0.01312245379660708
45-49	0.008486989598325098
50-54	0.005078560040764818
55-59	0.004004904730133329
60-64	0.010804721697466088
65-69	0.01436653058511658
70-74	0.01842256175861331
75-79	0.007822345834600843
80-84	0.010855848140829493
85-89	0.00899825403195914
90-94	0.009833319273561408
95-99	0.00913459121426155
100-101	0.00656122689830354
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1173561.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	27.744780026262916
#Duplication Level	Relative count
1	100.0
2	7.096176297134329
3	2.976398233177272
4	2.1002082154849737
5	1.4827831068638897
6	1.1397691576299542
7	0.898455835449589
8	0.6836207830346505
9	0.5446098667661607
10++	3.433750165489186
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GCTGC	144555	2.6132085	6.05869	95-97
ACACA	294950	2.4113867	7.513011	6
CAAAG	280610	2.343529	5.2140207	4
GCCGA	123410	2.2699625	6.1069994	90-94
GACGC	123130	2.2648122	5.8553452	95-97
CTGCC	126430	2.2373936	5.6833453	90-94
CGCTG	122660	2.2173994	5.681666	95-97
CTTTG	278820	2.2105942	9.17384	9
CGACG	120160	2.2101831	6.140827	95-97
TACAC	266775	2.1435628	9.257242	5
CCGAC	114930	2.0694408	5.653369	95-97
CACAT	253915	2.0402315	5.4201355	7
TGCCG	111640	2.018184	5.7934012	90-94
GCTTT	232220	1.841131	5.288351	1
GCCAA	142430	1.7466056	5.2765436	1
ATACA	317120	1.7353557	5.29147	6
GAGAG	130555	1.6706456	5.361625	7
TGGCT	136625	1.6531701	5.363149	6
GACTC	128735	1.5515387	5.6225452	7
TTGAG	188005	1.5492823	5.738669	9
GAGTC	121945	1.501339	7.851201	9
TATAC	270050	1.4523847	5.8022876	5
AAGAC	173570	1.4495788	6.0323825	5
GTGTA	173930	1.4332952	10.801375	1
CCATG	117695	1.4184827	5.5290785	9
GACTT	168655	1.360541	7.3311806	7
GTCTT	168405	1.3351808	6.3537135	1
TCATA	246075	1.3234425	6.352694	2
TATGA	240690	1.3223433	6.0444427	4
TGAGT	159165	1.3116221	6.002501	8
ACTCA	161995	1.3016454	5.0266886	8
AACAC	157250	1.2856097	5.151748	5
GTGTT	156330	1.2661235	6.0543694	1
ATGAG	147815	1.2393872	5.0257263	7
GATTG	149090	1.2285976	6.166219	7
GTTCT	150545	1.1935797	5.0383563	1
ACATG	140580	1.1538873	5.493132	8
TGGAC	93345	1.1492269	7.8117704	5
GTCCA	92535	1.1152495	8.0156	1
ACACT	138285	1.1111333	5.427859	6
GGACT	89135	1.0973951	7.833122	6
AGACT	127040	1.0427501	5.7636113	6
GAGTA	119965	1.0058728	5.19412	1
TGTAT	185040	0.9991357	5.1940126	2
GTATG	119215	0.9824084	5.4844956	3
GTATA	143225	0.7868737	7.5103426	1
>>END_MODULE