##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-126_TCCTGAGC-ACTGCATA_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1173561	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.459666774884305	34.0	31.0	34.0	31.0	34.0
2	32.64483993588744	34.0	31.0	34.0	31.0	34.0
3	32.75369750698941	34.0	31.0	34.0	31.0	34.0
4	36.260237857256676	37.0	37.0	37.0	35.0	37.0
5	35.993648391519486	37.0	35.0	37.0	35.0	37.0
6	36.08698653073849	37.0	36.0	37.0	35.0	37.0
7	36.083117963190666	37.0	36.0	37.0	35.0	37.0
8	36.10196231810703	37.0	36.0	37.0	35.0	37.0
9	37.966683453182235	39.0	38.0	39.0	35.0	39.0
10-14	38.1501038292854	39.2	38.2	39.4	35.2	39.4
15-19	39.289110493617294	40.0	39.0	41.0	36.0	41.0
20-24	39.220603956675454	40.0	39.0	41.0	36.0	41.0
25-29	39.02916542045961	40.0	39.0	41.0	36.0	41.0
30-34	38.83644735978787	40.0	38.0	41.0	35.0	41.0
35-39	38.60735862899329	40.0	38.0	41.0	34.6	41.0
40-44	38.36677565120177	40.0	38.0	41.0	34.0	41.0
45-49	38.18512459088194	40.0	38.0	41.0	33.4	41.0
50-54	38.125595686973234	40.0	37.4	41.0	33.4	41.0
55-59	37.737159465933175	39.8	36.6	41.0	33.0	41.0
60-64	37.18945960201472	39.0	35.4	40.8	32.0	41.0
65-69	36.46145364407985	37.8	35.0	40.0	31.0	41.0
70-74	35.888773229512566	36.6	35.0	39.2	31.0	41.0
75-79	34.716162517329735	35.2	33.6	37.4	30.4	39.2
80-84	34.52436831148956	35.0	34.0	36.6	31.0	38.4
85-89	33.90530036359422	35.0	34.0	35.6	30.4	36.8
90-94	33.50102755630087	35.0	34.0	35.0	30.0	36.0
95-99	33.23042449433817	35.0	34.0	35.0	29.2	35.6
100-101	32.88422672532574	35.0	33.5	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	2.0
14	9.0
15	35.0
16	98.0
17	197.0
18	301.0
19	541.0
20	844.0
21	1273.0
22	1734.0
23	2360.0
24	3252.0
25	4510.0
26	5716.0
27	7405.0
28	9539.0
29	12138.0
30	15344.0
31	19690.0
32	25174.0
33	34397.0
34	50090.0
35	79425.0
36	148102.0
37	286049.0
38	382664.0
39	82572.0
40	99.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.026584898441584	23.700770560712225	10.963639725587337	24.30900481525886
2	15.384353167562335	23.40556626493703	39.855346651599675	21.35473391590096
3	15.81247161417259	27.433853033630122	33.00211919107741	23.751556161119876
4	11.3100213793744	18.64879627049638	44.02625854131145	26.01492380881778
5	10.534688865768375	39.34844460577678	38.72632100078309	11.390545527671762
6	25.352665945783816	43.98348275036406	19.483435458403953	11.18041584544817
7	23.894113727364832	33.877233479981015	26.704193476095405	15.524459316558747
8	21.06426508720041	38.68167057357905	24.23137783208542	16.02268650713512
9	22.743853962427178	15.006378023809585	25.509198073214773	36.74056994054847
10-14	20.337144809686077	26.000881079040628	32.92682698215091	20.73514712912239
15-19	19.59934434633347	31.815546449633352	28.74147967221407	19.843629531819108
20-24	19.50679688092485	30.15269016397214	30.466148525034658	19.874364430068347
25-29	19.560018933960738	30.687249252434118	29.91495910738341	19.837772706221735
30-34	19.43751442294746	30.400505438026403	29.98207378890702	20.17990635011912
35-39	19.61569269055013	30.729325264500694	29.83314129675329	19.821840748195886
40-44	19.337082236294105	30.426271035146353	30.302734230130397	19.933912498429144
45-49	19.52312551708521	30.22736061974517	30.253108308762194	19.996405554407424
50-54	19.251148877586115	29.856613461327612	30.691311329243398	20.200926331842876
55-59	19.473685018139943	29.879324487194285	30.671466930640268	19.975523564025504
60-64	19.038849721668903	29.53189630388988	30.92629707952702	20.502956894914195
65-69	19.418384172389317	29.71549031285341	30.313890044965657	20.552235469791615
70-74	19.237533928037625	29.4356978685881	30.355346663335357	20.97142154003892
75-79	19.428459143479483	29.75365035498474	29.910065533153695	20.90782496838208
80-84	19.28253482525043	29.585600865196938	29.728073414946017	21.403790894606615
85-89	19.31708798542779	29.469095413326862	29.63272256769709	21.581094033548254
90-94	19.132179650329782	28.961185948088524	30.121208805648198	21.785425595933493
95-99	19.305884263711626	28.942133219216426	29.63266320327994	22.11931931379201
100-101	19.12317260166817	28.115934372365125	30.56344346968051	22.19744955628619
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.33558971370044
2	36.739087083463296
3	39.564027775292466
4	37.324945188192174
5	21.92523439344014
6	36.533081791231986
7	39.41857304392358
8	37.08695159433553
9	59.48442390297565
10-14	41.07229193880846
15-19	39.44297387815258
20-24	39.38116131099319
25-29	39.397791640182476
30-34	39.61742077306658
35-39	39.437533438746016
40-44	39.27099473472325
45-49	39.519531071492636
50-54	39.45207520942899
55-59	39.44920858216545
60-64	39.541806616583095
65-69	39.970619642180935
70-74	40.20895546807654
75-79	40.336284111861566
80-84	40.68632571985704
85-89	40.89818201897605
90-94	40.91760524626328
95-99	41.42520357750363
100-101	41.32062215795436
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	0.5
5	0.5
6	2.5
7	6.0
8	13.5
9	17.5
10	27.0
11	40.5
12	58.0
13	94.5
14	157.5
15	238.0
16	354.0
17	511.0
18	757.5
19	1154.0
20	1702.5
21	2469.0
22	3493.5
23	4887.0
24	6547.5
25	8440.5
26	10886.5
27	13767.0
28	16830.0
29	20091.5
30	23772.5
31	27803.5
32	31621.5
33	35996.5
34	41675.0
35	48468.0
36	56488.5
37	63569.0
38	68013.5
39	68623.0
40	65655.0
41	60947.5
42	54935.5
43	49590.0
44	45715.5
45	42079.0
46	38536.0
47	35037.5
48	31206.5
49	26755.0
50	22903.5
51	20652.5
52	18473.5
53	16247.5
54	14261.5
55	12397.0
56	11095.0
57	9509.5
58	7605.5
59	6357.0
60	5270.0
61	4333.5
62	3506.0
63	2412.0
64	1697.5
65	1385.5
66	1104.0
67	833.5
68	647.5
69	666.0
70	708.5
71	572.0
72	481.0
73	413.0
74	307.0
75	243.0
76	165.5
77	101.0
78	57.5
79	41.5
80	29.5
81	19.0
82	10.0
83	6.0
84	3.5
85	2.5
86	2.0
87	1.0
88	1.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.011503449756765946
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0012440767885095023
20-24	6.816859115120561E-4
25-29	0.01871227827100594
30-34	0.0071406599230887866
35-39	0.04436071069164705
40-44	0.053785018418301224
45-49	0.05455191506875229
50-54	0.05398952419175484
55-59	0.0438324041102252
60-64	0.05465416795547909
65-69	0.04536619741112733
70-74	0.0040730733212845345
75-79	0.0014997090053265234
80-84	1.3633718230241122E-4
85-89	0.003357303114196876
90-94	0.002760827941623827
95-99	0.0023518163947165932
100-101	4.2605369469503504E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1173561.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	28.821804381576676
#Duplication Level	Relative count
1	100.0
2	7.298482865590616
3	3.1075808249721293
4	2.121201105133052
5	1.563181619892395
6	1.1814744801512287
7	0.9142794823324124
8	0.7076729193931462
9	0.6161843827250254
10++	3.6668120789103775
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	152875	2.7875147	6.4125266	95-97
GAGCC	147125	2.6826696	6.1237273	95-97
CTCCG	156405	2.6653082	6.097671	95-97
CGAGC	138925	2.5331514	6.138462	95-97
GCCCA	140485	2.4320428	5.8287334	90-94
AGCCC	138470	2.3971598	5.620564	90-94
CAAAG	284195	2.3695896	5.206765	4
ACACA	294370	2.3302944	7.78744	6
GACTC	189360	2.2394571	5.280919	7
CTTTG	278020	2.2110534	9.142188	9
CCCAC	132950	2.185195	5.5148168	90-94
CATCT	279200	2.1416261	5.030717	95-97
TACAC	268225	2.090124	10.004287	5
CCACG	117905	2.0411434	5.511443	90-94
CACAT	253965	1.9790038	5.3459463	7
GCTTT	234045	1.8613266	5.479774	1
GAGAG	128350	1.7106996	5.706029	7
ATACA	316015	1.7089847	5.1206856	6
GCCAA	141940	1.7053117	5.0916176	1
TGGCT	136490	1.6735946	5.487146	6
TTGAG	184455	1.569631	5.5656323	9
AGACT	188145	1.5442046	5.797852	6
GAGTC	123075	1.5330764	8.050438	9
TATAC	270170	1.4382125	5.8897524	5
AAGAC	171360	1.4287827	5.6918674	5
CATGA	172960	1.4195735	5.0497427	9
GTCTT	177585	1.4123082	6.3667183	1
CCATG	118540	1.4019078	5.9689875	9
TATGA	243175	1.3634659	6.271456	4
TGAGT	160095	1.3623383	6.291764	8
GACTT	167360	1.3521328	7.113428	7
GTGTT	157580	1.3199689	6.3240695	1
TCATA	246025	1.3096801	6.213087	2
ATGAG	149240	1.2901402	5.205574	7
ACTCA	163175	1.2715293	5.1305976	8
GATTG	147875	1.2583512	6.1473627	7
GTGTA	147460	1.2548198	11.070651	1
AACAC	157130	1.243874	5.0201936	5
GTATG	140830	1.1984015	5.697656	3
GTTCT	149500	1.1889522	5.132709	1
TGGAC	92525	1.1525321	7.603527	5
ACATG	139785	1.1472889	5.571032	8
ACACC	99155	1.1310301	5.3257556	6
GTCCA	94050	1.112278	7.6671033	1
ACACT	140075	1.0915242	5.463066	6
GGACT	87280	1.087198	7.3740315	6
GAGTA	119130	1.0298473	5.3654604	1
TGTAT	186035	1.0267756	5.189244	2
CATAC	118720	0.92511696	5.168378	3
GTATA	143665	0.8055202	7.0307126	1
>>END_MODULE