##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-125_TCCTGAGC-GTAAGGAG_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	843328	
Filtered Sequences	0	
Sequence length	101	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.514609973818015	31.0	31.0	34.0	30.0	34.0
2	31.72191128481445	33.0	31.0	34.0	30.0	34.0
3	31.86051571867648	34.0	31.0	34.0	30.0	34.0
4	35.39925509410336	37.0	35.0	37.0	33.0	37.0
5	35.263912736207025	37.0	35.0	37.0	32.0	37.0
6	35.41384490969113	37.0	35.0	37.0	33.0	37.0
7	35.427167128329664	37.0	35.0	37.0	33.0	37.0
8	35.47271049935494	37.0	35.0	37.0	33.0	37.0
9	37.24289718828261	39.0	37.0	39.0	34.0	39.0
10-14	37.357014115504285	39.2	37.2	39.4	33.2	39.4
15-19	38.380530232602254	40.0	38.0	41.0	33.4	41.0
20-24	38.269050713364194	40.0	38.0	41.0	33.6	41.0
25-29	38.07991599946877	40.0	38.0	41.0	33.0	41.0
30-34	37.805817902405714	40.0	38.0	41.0	32.2	41.0
35-39	37.481189999620554	40.0	37.0	41.0	31.4	41.0
40-44	37.424890434089704	40.0	37.0	41.0	31.0	41.0
45-49	37.25203100098658	40.0	36.8	41.0	31.0	41.0
50-54	36.583163846095474	38.6	35.4	40.2	30.2	40.6
55-59	37.22670562343477	39.8	36.0	41.0	31.0	41.0
60-64	36.764753690141916	39.0	35.2	41.0	30.8	41.0
65-69	36.090274721104954	37.8	35.0	40.0	29.6	41.0
70-74	35.26010733664719	36.4	34.2	39.2	29.0	40.8
75-79	34.31015500493284	35.4	34.0	37.6	28.4	39.4
80-84	33.40437647036503	35.0	33.2	36.4	27.0	38.0
85-89	32.652538988388855	35.0	33.0	35.4	26.0	36.6
90-94	32.076127200804436	35.0	32.0	35.0	25.0	36.0
95-99	31.59418494346209	34.4	31.6	35.0	23.6	35.2
100-101	30.710595995864004	34.0	30.0	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	2.0
6	22.0
7	89.0
8	213.0
9	251.0
10	289.0
11	481.0
12	625.0
13	945.0
14	1198.0
15	1489.0
16	1683.0
17	1970.0
18	2209.0
19	2483.0
20	2864.0
21	3261.0
22	3683.0
23	4197.0
24	4768.0
25	5527.0
26	6614.0
27	7790.0
28	9370.0
29	11279.0
30	13975.0
31	17697.0
32	22897.0
33	30572.0
34	43346.0
35	65734.0
36	111699.0
37	197981.0
38	224498.0
39	41599.0
40	28.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.71619847199669	23.813697941192867	10.415735041711098	24.054368545099347
2	15.180172700099634	23.75017791905869	40.00948901646345	21.060160364378234
3	15.568367953296558	27.978655141043784	32.92381631275745	23.52916059290221
4	11.159437135578022	18.76645681021354	44.16450405400917	25.90960200019926
5	10.750365628436107	39.02705472505809	38.32331635963367	11.89926328687213
6	25.28468212719181	43.679837402423075	19.628155278788675	11.407325191596437
7	23.620926813072142	34.01318597922497	26.913982829768056	15.45190437793483
8	20.932146410356445	38.158261403698084	24.748695898617456	16.16089628732801
9	22.531871019457597	15.21911585268243	26.192991755215395	36.05602137264458
10-14	20.271683356738095	26.25368829509871	32.89507418569346	20.57955416246974
15-19	19.522197286174713	31.952905265659837	28.895196567665472	19.629700880499982
20-24	19.468406464704927	30.319831396776188	30.51388640156174	19.697875736957148
25-29	19.451456499487257	30.92076741448218	29.97162607896317	19.656150007067392
30-34	19.336748754079746	30.530560205209838	30.148827409628982	19.983863631081437
35-39	19.501568271699366	30.839206199564796	30.023586332022983	19.635639196712855
40-44	19.256479829987477	30.626707715077227	30.418793404424882	19.698019050510418
45-49	19.42940763509411	30.414769277474196	30.382015596539162	19.77380749089253
50-54	19.170147115674236	30.064059235226175	30.78241916701649	19.9833744820831
55-59	19.372684231584074	30.001520227925095	30.84030183520682	19.78549370528401
60-64	19.004968870441743	29.543860065223836	31.20130447672695	20.24986658760747
65-69	19.352974131203986	29.741264602874395	30.69925395896988	20.206507306951742
70-74	19.254150998084903	29.313961613615263	30.746015037094633	20.6858723512052
75-79	19.262531185214858	29.640818899609876	30.365072464489945	20.73157745068532
80-84	19.201977327429752	29.320221355933167	30.320459485280093	21.157341831356984
85-89	19.330304752345434	29.17169084756311	30.144587308800467	21.35341709129099
90-94	19.236057432664	28.695080673221213	30.608862420654393	21.459999473460385
95-99	19.610613944647667	28.604033890964597	30.02898088741427	21.756371276973464
100-101	19.819477952960863	27.880831494770696	30.642440528106736	21.657250024161705
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.77056701709604
2	36.240333064477866
3	39.09752854619876
4	37.06903913577728
5	22.649628915308238
6	36.69200731878825
7	39.072831191006976
8	37.09304269768446
9	58.587892392102184
10-14	40.851237519207835
15-19	39.15189816667469
20-24	39.16628220166207
25-29	39.107606506554646
30-34	39.32061238516118
35-39	39.13720746841222
40-44	38.954498880497894
45-49	39.20321512598664
50-54	39.15352159775733
55-59	39.158177936868086
60-64	39.254835458049214
65-69	39.55948143815572
70-74	39.94002334929011
75-79	39.994108635900176
80-84	40.35931915878674
85-89	40.68372184363642
90-94	40.69605690612439
95-99	41.366985221621135
100-101	41.47672797712257
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	2.0
2	4.5
3	7.0
4	7.0
5	5.0
6	7.5
7	15.0
8	20.5
9	27.0
10	43.0
11	64.0
12	89.0
13	118.5
14	150.5
15	221.5
16	343.5
17	486.0
18	678.5
19	997.0
20	1445.0
21	2072.0
22	2868.5
23	4047.0
24	5336.0
25	6722.5
26	8496.0
27	10345.0
28	12402.0
29	14825.5
30	17354.0
31	20082.5
32	23050.5
33	26457.0
34	30597.5
35	35207.0
36	40956.5
37	46196.5
38	48754.5
39	48868.0
40	47052.0
41	43773.5
42	39546.5
43	35828.0
44	33036.5
45	30126.5
46	27304.5
47	24503.5
48	21534.5
49	18665.0
50	16104.0
51	14227.5
52	12609.0
53	11193.5
54	9845.5
55	8532.5
56	7419.5
57	6287.0
58	5221.5
59	4364.0
60	3512.0
61	2857.5
62	2325.5
63	1624.5
64	1130.5
65	929.0
66	765.5
67	582.0
68	447.5
69	449.0
70	460.5
71	374.0
72	301.5
73	251.5
74	192.0
75	146.5
76	109.0
77	77.0
78	55.5
79	33.5
80	26.0
81	24.5
82	20.0
83	13.0
84	12.0
85	11.0
86	8.5
87	9.0
88	7.0
89	4.0
90	4.0
91	4.0
92	2.0
93	2.0
94	3.0
95	1.5
96	1.5
97	2.0
98	1.5
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.031660279274493434
2	0.029407300599529482
3	0.02620569932458071
4	0.025138498899597784
5	0.030711656674508616
6	0.0022529786749639524
7	9.48622599984822E-4
8	0.0032016012749487744
9	8.300447749867194E-4
10-14	0.006189762464900964
15-19	0.022932951354633073
20-24	0.019565341124686955
25-29	0.002181831979965091
30-34	0.001849814069970403
35-39	0.005098846474918419
40-44	0.011383471199817865
45-49	0.007588980799878576
50-54	0.005454579949912727
55-59	0.0039604993549366314
60-64	0.009249070349852014
65-69	0.012284662669803445
70-74	0.015794566289747288
75-79	0.0070909539348865454
80-84	0.010529710859831525
85-89	0.00863246565986188
90-94	0.010268839644835699
95-99	0.009794528344843288
100-101	0.006106757987402292
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	843328.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	28.958354720596798
#Duplication Level	Relative count
1	100.0
2	7.3631415428337466
3	3.500036126297536
4	2.334360925796585
5	1.567279208111558
6	1.1512246814864768
7	0.8423448375520821
8	0.6412417812673105
9	0.5412923580838612
10++	2.4800703258592036
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GTCTC	191085	3.1178272	5.8463984	90-94
GCTGC	115020	2.9191873	7.4125133	90-94
CTGTC	177755	2.9003289	5.4079976	90-94
GACGC	101875	2.643483	8.013169	95-97
CGCTG	100460	2.549657	7.20158	95-97
ACACA	227355	2.5487518	7.1541133	6
GCCGA	97460	2.528921	7.3394322	90-94
CTGCC	101000	2.4536924	6.8762264	90-94
CGACG	91915	2.3850377	7.3880577	95-97
GACTC	138760	2.3147814	5.5873156	85-89
TACAC	209975	2.3023448	8.749009	5
TGCCG	89720	2.2770777	6.954616	90-94
CCGAC	91320	2.2682188	6.754716	95-97
CACAT	199430	2.1867206	5.0513906	7
CTTTG	197605	2.1654592	8.736624	9
CTGAC	123135	2.0541265	5.3376923	95-97
TGACG	114280	1.9916164	5.4466486	95-97
GACGA	105830	1.8856649	5.358346	95-97
ATACA	249420	1.8779365	5.505894	6
GCTTT	167860	1.8394977	5.4645047	1
GCCAA	101040	1.7232935	5.3444633	1
GAGAG	88475	1.6468959	5.036912	7
TATAC	213010	1.5686619	5.2259336	5
GAGTC	87770	1.5296131	8.0625725	9
TTGAG	129735	1.5185157	5.2447386	9
GTGTA	123765	1.4486386	10.180008	1
AAGAC	120515	1.4114124	5.442125	5
CCATG	81970	1.3674159	5.4120474	9
TATGA	174085	1.3393081	5.616243	4
GTCTT	120560	1.3211597	6.4744816	1
TGAGT	112310	1.3145604	6.6581726	8
GACTT	116810	1.3087369	6.7643533	7
TCATA	175730	1.2941222	5.7832108	2
ATGAG	106055	1.2691516	5.135825	7
GTGTT	110030	1.259659	5.680979	1
GATTG	105205	1.2313982	5.891747	7
TGGAC	66720	1.162764	7.48116	5
GTTCT	105700	1.158316	5.0445676	1
ACATG	98800	1.1317475	5.349507	8
GTCCA	66325	1.1064274	8.156651	1
GGACT	63255	1.1023775	7.631328	6
ACACT	98245	1.0772419	5.056618	6
GAGTA	84135	1.0068368	5.2127194	1
GTATG	85715	1.0032727	5.1266646	3
AGACT	87320	1.0002449	5.3992786	6
GTATA	100990	0.77695805	7.2136774	1
>>END_MODULE