##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-125_TCCTGAGC-GTAAGGAG_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	843328	
Filtered Sequences	0	
Sequence length	101	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.356563519769296	33.0	31.0	34.0	31.0	34.0
2	32.542855211732565	34.0	31.0	34.0	31.0	34.0
3	32.652715195036805	34.0	31.0	34.0	31.0	34.0
4	36.19144271268119	37.0	35.0	37.0	35.0	37.0
5	35.8968574504819	37.0	35.0	37.0	35.0	37.0
6	36.00335693822569	37.0	35.0	37.0	35.0	37.0
7	35.99842172914928	37.0	35.0	37.0	35.0	37.0
8	36.01920486453669	37.0	35.0	37.0	35.0	37.0
9	37.858958791834254	39.0	38.0	39.0	35.0	39.0
10-14	38.03436053350535	39.2	38.0	39.4	35.2	39.4
15-19	39.139025385140776	40.0	38.8	41.0	36.0	41.0
20-24	39.066340972907334	40.0	39.0	41.0	36.0	41.0
25-29	38.86322356188814	40.0	38.0	41.0	35.2	41.0
30-34	38.65179787698262	40.0	38.0	41.0	34.6	41.0
35-39	38.41556357668665	40.0	38.0	41.0	34.0	41.0
40-44	38.170785981255214	40.0	38.0	41.0	33.4	41.0
45-49	37.98778292669045	40.0	37.6	41.0	33.0	41.0
50-54	37.938992183349775	40.0	37.0	41.0	33.0	41.0
55-59	37.54978110533506	39.4	36.4	41.0	32.6	41.0
60-64	36.984256422174994	38.8	35.2	40.4	31.6	41.0
65-69	36.242409833421874	37.6	35.0	40.0	31.0	41.0
70-74	35.68321957767322	36.6	34.6	39.0	30.6	40.8
75-79	34.53337681186916	35.2	33.6	37.4	29.4	39.2
80-84	34.37244156484784	35.0	34.0	36.4	30.2	38.2
85-89	33.75516169272216	35.0	34.0	35.6	30.0	36.8
90-94	33.33924973438567	35.0	34.0	35.0	29.0	36.0
95-99	33.05640391401685	35.0	33.6	35.0	29.0	35.4
100-101	32.69530182799575	35.0	33.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	4.0
14	11.0
15	33.0
16	82.0
17	141.0
18	274.0
19	448.0
20	663.0
21	1019.0
22	1433.0
23	1947.0
24	2675.0
25	3466.0
26	4558.0
27	5899.0
28	7505.0
29	9541.0
30	12060.0
31	15639.0
32	20200.0
33	27254.0
34	39146.0
35	62410.0
36	111452.0
37	209873.0
38	256175.0
39	49362.0
40	57.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.06278932989299	23.88619848979282	10.86564183805115	24.185370342263035
2	15.382827352045094	23.691404188502787	39.738832299786644	21.18693615966547
3	15.783301396372467	27.665392350307354	33.318471579266905	23.232834674053272
4	11.46066536389163	18.87201658192305	43.80502011079912	25.862297943386203
5	10.676984518479168	39.24890434089702	38.371191280261066	11.702919860362753
6	25.25932970327085	44.00126641117098	19.429688092889126	11.309715792669046
7	23.483271040449267	34.1310854139789	26.913727517644382	15.471916027927449
8	20.873966001366018	38.210874060863624	24.731421226379297	16.18373871139106
9	22.3563073916673	15.093059877058511	26.21637132883054	36.33426140244365
10-14	20.149929801927602	26.232711353115278	32.95073802838279	20.666620816574337
15-19	19.476989204062246	31.99529192574277	28.85239385607219	19.675325014122798
20-24	19.396170535601847	30.354803052017242	30.55342180385669	19.69560460852422
25-29	19.438046655183065	30.90698328647068	30.045380949014984	19.609589109331274
30-34	19.320437119692777	30.500141355226635	30.12450644257278	20.05491508250781
35-39	19.458974678363614	30.862382926728	29.991494207875864	19.68714818703252
40-44	19.23215550511995	30.62458549803708	30.395750754260103	19.74750824258286
45-49	19.4004142519961	30.383953085925846	30.39821390902449	19.81741875305356
50-54	19.11048700025223	29.985115145843334	30.827949546679907	20.076448307224528
55-59	19.297013111220092	29.992239228221973	30.84034851583247	19.87039914472546
60-64	18.87835706750179	29.57149887597471	31.107168467116146	20.44297558940735
65-69	19.18730396739156	29.73816449419435	30.635157514128586	20.439374024285502
70-74	19.027200349105534	29.463202806125473	30.522851945575585	20.986744899193404
75-79	19.154360060428264	29.77327543477281	30.093938912433565	20.978425592365358
80-84	18.967437794621638	29.51235783398002	29.917064238633774	21.60314013276457
85-89	19.046601501958378	29.384761514006303	29.747195241545406	21.82144174248991
90-94	18.855106103076167	28.853794592830617	30.16447476860619	22.126624535487025
95-99	19.056133121216693	28.86263834776694	29.592856526238997	22.488372004777368
100-101	18.912273753509904	28.09061243075055	30.416575757000835	22.58053805873871
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.24815967215602
2	36.56976351171056
3	39.016136070425745
4	37.32296330727783
5	22.37990437884192
6	36.569045495939896
7	38.955187068376716
8	37.05770471275708
9	58.69056879411095
10-14	40.816550618501935
15-19	39.15231421818504
20-24	39.09177514412607
25-29	39.04763576451434
30-34	39.37535220220059
35-39	39.14612286539614
40-44	38.979663747702816
45-49	39.21783300504966
50-54	39.18693530747676
55-59	39.16741225594556
60-64	39.321332656909135
65-69	39.62667799167707
70-74	40.013945248298946
75-79	40.13278565279362
80-84	40.57057792738621
85-89	40.868043244448295
90-94	40.98173063856319
95-99	41.54450512599406
100-101	41.49281181224861
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	4.5
7	9.0
8	11.0
9	11.5
10	21.0
11	34.0
12	48.5
13	82.5
14	127.5
15	188.0
16	307.0
17	459.5
18	675.0
19	1007.5
20	1455.0
21	2128.5
22	3064.0
23	4188.5
24	5385.5
25	6758.5
26	8442.0
27	10258.5
28	12334.0
29	14743.5
30	17305.5
31	20102.5
32	23022.0
33	26136.5
34	30090.5
35	34839.0
36	40619.5
37	45736.0
38	48488.0
39	48769.5
40	46885.0
41	43515.0
42	39369.5
43	35588.0
44	32580.5
45	30015.5
46	27507.0
47	25094.0
48	22335.5
49	19110.0
50	16439.0
51	14681.0
52	13003.0
53	11517.0
54	10206.5
55	8707.0
56	7477.0
57	6440.0
58	5271.5
59	4307.0
60	3543.5
61	2863.0
62	2250.0
63	1602.5
64	1143.5
65	901.0
66	712.0
67	577.0
68	446.5
69	417.5
70	411.5
71	344.5
72	301.5
73	264.0
74	196.0
75	149.5
76	105.5
77	64.0
78	44.5
79	30.5
80	21.5
81	12.0
82	6.5
83	6.5
84	4.0
85	2.0
86	2.0
87	1.5
88	0.0
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.013636449874781818
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0012806405099795098
20-24	4.505957349927905E-4
25-29	0.018355847309706304
30-34	0.007399256279881612
35-39	0.04422952872429233
40-44	0.05324144342414813
45-49	0.0544746528041284
50-54	0.05428492828413144
55-59	0.04394494194429688
60-64	0.05506754192911892
65-69	0.04221370569932458
70-74	0.003557334749943083
75-79	0.0016600895499734388
80-84	7.114669499886166E-5
85-89	0.00343875692494498
90-94	0.00301187675495181
95-99	0.002181831979965091
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	843328.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	29.865438492231938
#Duplication Level	Relative count
1	100.0
2	7.484430133819141
3	3.573352378300842
4	2.3531832735151887
5	1.6281026249372061
6	1.2020118264406288
7	0.8927328519637097
8	0.6591092039243931
9	0.5356397111782258
10++	2.7199602960846856
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	121710	3.1433234	8.302067	95-97
GTCTC	190480	3.0927446	5.75016	90-94
GAGCC	117295	3.0293	7.8011875	95-97
CTCCG	125485	2.998703	7.6045933	95-97
CTGTC	178855	2.9039943	5.397633	85-89
CGAGC	111850	2.888676	7.767797	95-97
AGAAG	228990	2.8304858	5.0279655	5
GCCCA	110050	2.677747	7.1212	90-94
AGCCC	109930	2.674827	7.0763683	90-94
TCTCC	171405	2.6220126	5.567604	95-97
ACACA	227725	2.4985528	6.6918206	6
CCCAC	103875	2.3812616	6.7047396	90-94
CAAAG	201045	2.3412855	5.3411913	4
GACTC	141490	2.339151	5.6995416	85-89
CATCT	218635	2.3137789	5.9178905	95-97
ATCTC	218525	2.3126147	5.789613	95-97
CCACG	93350	2.2714007	6.717712	90-94
TCCGA	137290	2.2697153	5.4720626	95-97
TACAC	210210	2.265131	8.562583	5
CTTTG	196900	2.172168	8.809011	9
GAGAC	113290	2.0241618	5.558697	95-97
CGAGA	112555	2.0110297	5.500945	95-97
ATACA	250995	1.8710951	5.504483	6
ACGAG	104625	1.8693435	5.3247986	95-97
GCTTT	166710	1.8391169	5.4830775	1
GCCAA	100265	1.6877948	5.0362616	1
TGGCT	97910	1.6873497	5.096755	6
GAGAG	88025	1.6693331	5.6729374	7
AGACT	139675	1.5975041	5.567278	6
TATAC	215365	1.5767692	5.398935	5
TTGAG	131780	1.5711527	5.531803	9
GAGTC	87145	1.5291787	8.141672	9
AAGAC	120750	1.4062037	5.510574	5
CCATG	82975	1.371765	5.5625997	9
GTCTT	123610	1.3636448	5.7933393	1
TATGA	174300	1.3544837	5.673972	4
TGAGT	111320	1.3272173	6.7052145	8
GACTT	117470	1.3195093	6.829185	7
TCATA	177885	1.3023638	5.413967	2
GTGTT	109935	1.2872624	5.8992863	1
GATTG	107130	1.2772619	6.0520344	7
ATGAG	104655	1.2704766	5.208778	7
GTGTA	103740	1.2368444	10.37156	1
GTATG	101965	1.2156819	5.3872933	3
GTTCT	107135	1.1818955	5.4135365	1
TGGAC	65730	1.1533986	7.1122656	5
GTCCA	67385	1.114027	7.5194855	1
ACATG	97375	1.1137066	5.2512074	8
GGACT	62915	1.1040024	7.418535	6
TGTAT	133430	1.0183383	5.095952	2
GTATA	100350	0.77981883	6.869353	1
>>END_MODULE