##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-123_TCCTGAGC-TATCCTCT_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1279393	
Filtered Sequences	0	
Sequence length	101	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.60490873406373	33.0	31.0	34.0	30.0	34.0
2	31.802815084966074	33.0	31.0	34.0	30.0	34.0
3	31.945386601302335	34.0	31.0	34.0	30.0	34.0
4	35.46955079479097	37.0	35.0	37.0	33.0	37.0
5	35.34535752501382	37.0	35.0	37.0	33.0	37.0
6	35.486077382008496	37.0	35.0	37.0	33.0	37.0
7	35.498817017132346	37.0	35.0	37.0	33.0	37.0
8	35.5484530554724	37.0	35.0	37.0	33.0	37.0
9	37.335426252918374	39.0	37.0	39.0	34.0	39.0
10-14	37.45350521692709	39.2	37.2	39.4	34.0	39.4
15-19	38.50445672283653	40.0	38.0	41.0	34.0	41.0
20-24	38.39734264608295	40.0	38.0	41.0	34.0	41.0
25-29	38.21377575146964	40.0	38.0	41.0	33.4	41.0
30-34	37.95312527112467	40.0	38.0	41.0	32.8	41.0
35-39	37.646133439842174	40.0	37.6	41.0	31.8	41.0
40-44	37.6075057468659	40.0	37.6	41.0	31.8	41.0
45-49	37.44837356465136	40.0	37.0	41.0	31.4	41.0
50-54	36.77564298069474	39.0	36.4	40.2	30.8	40.6
55-59	37.40851356854383	40.0	36.6	41.0	31.6	41.0
60-64	36.96220285713616	39.0	35.4	41.0	31.0	41.0
65-69	36.292975653298086	38.0	35.0	40.2	30.2	41.0
70-74	35.46603287652816	36.6	34.6	39.2	29.4	41.0
75-79	34.51344864322378	35.4	34.0	38.0	29.0	39.6
80-84	33.596664824647306	35.0	33.6	36.4	27.6	38.2
85-89	32.82942848679022	35.0	33.0	35.4	26.2	36.8
90-94	32.26082228056586	35.0	32.6	35.0	25.4	36.0
95-99	31.777217633674717	35.0	32.0	35.0	24.4	35.2
100-101	30.931976335652923	34.5	30.5	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	2.0
6	40.0
7	121.0
8	275.0
9	359.0
10	425.0
11	617.0
12	1002.0
13	1449.0
14	1804.0
15	2055.0
16	2489.0
17	2777.0
18	3195.0
19	3540.0
20	4049.0
21	4611.0
22	5240.0
23	5990.0
24	6949.0
25	8000.0
26	9192.0
27	11149.0
28	13241.0
29	15832.0
30	19481.0
31	24618.0
32	32322.0
33	43226.0
34	61245.0
35	94495.0
36	162868.0
37	299634.0
38	362966.0
39	74062.0
40	73.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.34421940618151	23.837340678982567	10.733785633918009	24.084654280917913
2	15.10278982524205	23.616053242885478	40.25648784294225	21.024669088930224
3	15.736179914433688	27.685909705021395	32.940372538717504	23.637537841827417
4	11.081904973374375	18.4940787205314	44.24716836417569	26.17684794191853
5	10.604184063777417	39.29487359923938	38.87632648082643	11.22461585615678
6	25.43151862195428	43.844386760118404	19.581561963361853	11.142532654565464
7	23.856209150326798	33.97418761812307	26.709140165673222	15.460463065876912
8	21.045769616778337	38.691490459321976	24.28534803230355	15.977391891596138
9	22.739309732465834	15.200470850125217	25.729723899239325	36.33049551816963
10-14	20.329954650738145	26.193857463743104	32.85587476800088	20.62031311751787
15-19	19.517355682031397	32.0301926324348	28.831430983801365	19.62102070173244
20-24	19.47672541475794	30.35711186601164	30.52067133021954	19.645491389010886
25-29	19.506597084404632	30.815319624170744	30.03082302655326	19.647260264871363
30-34	19.352200822309822	30.550696834180652	30.117468588880886	19.97963375462864
35-39	19.497473595117686	30.80716294916752	30.038696884410705	19.656666571304086
40-44	19.25509789136495	30.55013873031006	30.442543982597233	19.75221939572776
45-49	19.446899784100342	30.421199751738843	30.362620749817477	19.769279714343334
50-54	19.241231431307682	30.034577452524395	30.744401682504634	19.979789433663285
55-59	19.42058062936689	30.043190925840985	30.78027249615975	19.755955948632376
60-64	19.096204556920547	29.71111320604573	31.040614997762017	20.152067239271705
65-69	19.440983602200255	29.913371501007713	30.531632792972914	20.114012103819118
70-74	19.433378846516757	29.676518683935722	30.50510565359417	20.38499681595335
75-79	19.743312749159696	30.0877823809896	30.040881732197295	20.128023137653404
80-84	19.842089760755137	30.046580112352594	29.870743862225176	20.24058626466709
85-89	20.021318201516724	30.0228940849964	29.73576436289595	20.220023350590925
90-94	20.05901213074034	29.71058947718623	30.127970618068662	20.10242777400477
95-99	20.416861505230898	29.63833428622485	29.60615954077352	20.33864466777074
100-101	20.58482009729414	29.005683894485845	30.23254995929467	20.176946048925345
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.42887368709943
2	36.12745891417227
3	39.3737177562611
4	37.2587529152929
5	21.828799919934198
6	36.57405127651974
7	39.316672216203706
8	37.02316150837447
9	59.06980525063547
10-14	40.95026776825602
15-19	39.13837638376384
20-24	39.12221680376883
25-29	39.153857349275995
30-34	39.33183457693846
35-39	39.154140166421776
40-44	39.00731728709271
45-49	39.21617949844368
50-54	39.22102086497097
55-59	39.176536577999265
60-64	39.24827179619225
65-69	39.554995706019376
70-74	39.8183756624701
75-79	39.8713358868131
80-84	40.08267602542223
85-89	40.24134155210765
90-94	40.16143990474511
95-99	40.755506173001635
100-101	40.761766146219486
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	1.5
2	2.0
3	3.5
4	9.5
5	14.5
6	17.5
7	23.5
8	22.5
9	35.5
10	50.0
11	62.0
12	97.5
13	140.0
14	189.0
15	273.5
16	390.5
17	538.5
18	825.0
19	1167.5
20	1699.5
21	2608.0
22	3702.0
23	5190.0
24	7060.0
25	9185.5
26	11881.5
27	15079.0
28	18775.5
29	22666.0
30	26840.5
31	31422.0
32	36365.0
33	41858.5
34	47820.5
35	54734.5
36	63045.5
37	70522.0
38	74977.5
39	75332.5
40	71889.5
41	67205.5
42	61545.0
43	56016.5
44	51423.5
45	46586.5
46	42305.5
47	37864.0
48	32881.0
49	27979.0
50	23878.5
51	21045.5
52	18342.0
53	16031.0
54	14012.5
55	12013.5
56	10380.5
57	8864.5
58	7490.5
59	6215.0
60	4923.0
61	3907.5
62	3106.0
63	2170.0
64	1398.5
65	1083.5
66	826.0
67	557.5
68	399.5
69	435.0
70	470.0
71	346.5
72	276.0
73	245.5
74	180.0
75	123.5
76	88.5
77	62.0
78	36.0
79	21.0
80	12.0
81	12.5
82	14.0
83	12.0
84	9.0
85	8.5
86	11.5
87	9.5
88	8.5
89	8.0
90	4.5
91	5.0
92	4.0
93	2.5
94	4.0
95	4.5
96	3.0
97	1.0
98	0.0
99	1.5
100	2.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0351729296627385
2	0.03314071594889138
3	0.03009239537812072
4	0.028294667862025197
5	0.03478211933315252
6	0.0012505930546751467
7	5.471344614203767E-4
8	0.0022666999115987037
9	3.908103295859834E-4
10-14	0.006081008728357901
15-19	0.02133824399539469
20-24	0.017602097244552688
25-29	0.0019384192347464775
30-34	0.0016570357974445694
35-39	0.005096166697801223
40-44	0.011599250582111986
45-49	0.007425396262133683
50-54	0.004861680500049633
55-59	0.003923735709043273
60-64	0.009129329299128571
65-69	0.012537195373118346
70-74	0.015882531794374363
75-79	0.00726907213029929
80-84	0.010520614072454672
85-89	0.008644724490441952
90-94	0.010207965808785885
95-99	0.01006727409013493
100-101	0.005979398042665545
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1279393.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	26.979650203497968
#Duplication Level	Relative count
1	100.0
2	6.951198201943877
3	2.8983411365594702
4	1.950505251360292
5	1.5323246383099236
6	1.2340546045059866
7	0.914694770332074
8	0.7116301210554541
9	0.6212452623269883
10++	3.699150984893677
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GCTGC	154070	2.617562	5.801302	95-97
CTCTT	350380	2.5388358	5.026409	1
ACACA	306460	2.2848916	7.7375126	6
CTGCC	131330	2.1974099	5.261618	95-97
GACGC	125310	2.1492991	5.557269	95-97
GCCGA	124850	2.1414092	5.5152893	90-94
CGCTG	125585	2.133618	5.397518	95-97
CTTTG	288570	2.1231384	8.117259	9
CGACG	119685	2.0528197	5.3742456	95-97
TACAC	269315	1.9889332	9.550906	5
CCGAC	117250	1.9805789	5.223616	90-94
CACAT	264525	1.9535583	5.3148293	7
TGCCG	114035	1.9373901	5.1664248	90-94
GCTTT	242700	1.7856523	5.096615	1
GAGAG	149105	1.7384305	5.840651	7
TTGAG	199405	1.5039285	5.500977	9
GACTC	132605	1.4853513	5.2691045	7
GAGTC	125200	1.4239851	7.3248725	9
CCATG	123740	1.3860517	5.709169	9
AAGAC	182070	1.3783579	5.7362685	5
TATAC	274835	1.3459275	5.7632203	5
GTGTA	174010	1.3123974	10.555126	1
GTCTT	178150	1.3107291	6.4278736	1
GACTT	172450	1.2809215	6.0911417	7
TATGA	254670	1.2663661	5.9290915	4
TCATA	258400	1.2654417	6.1337433	2
TGAGT	166865	1.2585093	5.076759	8
GTGTT	164915	1.2320238	5.483078	1
GATTG	162745	1.2274358	5.9800477	7
GTTCT	165875	1.2204164	5.5106254	1
ACATG	152705	1.1451036	5.516746	8
TGGAC	99810	1.1352073	7.227652	5
GTCCA	101105	1.1325097	8.150553	1
ACACC	101060	1.125509	5.109243	6
ACACT	142950	1.055708	5.210968	6
GGACT	91070	1.0358013	6.67394	6
AGACT	133125	0.99827695	5.287515	6
TGTAT	198280	0.97662586	5.058129	2
GTATG	127875	0.9644435	5.5939465	3
GAGTA	126155	0.96056724	5.152571	1
CATAC	127585	0.9422351	5.352171	3
GTATA	149865	0.7452152	6.852976	1
>>END_MODULE