##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-123_TCCTGAGC-TATCCTCT_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1279393	
Filtered Sequences	0	
Sequence length	101	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.45651179895466	34.0	31.0	34.0	31.0	34.0
2	32.64391238657707	34.0	31.0	34.0	31.0	34.0
3	32.750857633268275	34.0	31.0	34.0	31.0	34.0
4	36.2567584784347	37.0	37.0	37.0	35.0	37.0
5	35.98534070453723	37.0	35.0	37.0	35.0	37.0
6	36.08031230435058	37.0	36.0	37.0	35.0	37.0
7	36.08058977968459	37.0	36.0	37.0	35.0	37.0
8	36.105578973778975	37.0	36.0	37.0	35.0	37.0
9	37.962156272544874	39.0	38.0	39.0	35.0	39.0
10-14	38.14891624387502	39.2	38.2	39.4	35.2	39.4
15-19	39.28934174252946	40.0	39.0	41.0	36.0	41.0
20-24	39.21960007597353	40.0	39.0	41.0	36.0	41.0
25-29	39.027457708460176	40.0	38.8	41.0	36.0	41.0
30-34	38.8351869988346	40.0	38.0	41.0	35.0	41.0
35-39	38.61670245186584	40.0	38.0	41.0	34.8	41.0
40-44	38.38345324696946	40.0	38.0	41.0	34.0	41.0
45-49	38.215830475858475	40.0	38.0	41.0	33.6	41.0
50-54	38.16680660281868	40.0	37.6	41.0	33.6	41.0
55-59	37.784416359945695	39.8	36.8	41.0	33.0	41.0
60-64	37.23842025085334	39.0	35.6	40.8	32.0	41.0
65-69	36.50128209236724	37.8	35.0	40.0	31.0	41.0
70-74	35.92581435102427	36.6	35.0	39.2	31.0	41.0
75-79	34.748186210179355	35.2	33.8	37.4	30.4	39.2
80-84	34.546605147909986	35.0	34.0	36.6	31.0	38.4
85-89	33.91985808895312	35.0	34.0	35.8	30.6	36.8
90-94	33.51578053029836	35.0	34.0	35.0	30.0	36.0
95-99	33.24245435139945	35.0	34.0	35.0	29.2	35.6
100-101	32.90620473927871	35.0	33.5	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	5.0
14	12.0
15	31.0
16	90.0
17	210.0
18	348.0
19	586.0
20	879.0
21	1326.0
22	1989.0
23	2588.0
24	3652.0
25	4687.0
26	6093.0
27	7849.0
28	10192.0
29	13142.0
30	16591.0
31	20869.0
32	27308.0
33	36965.0
34	53124.0
35	85826.0
36	159407.0
37	316967.0
38	420475.0
39	88117.0
40	65.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.756983975994864	23.87491568267139	11.124806841994603	24.24329349933914
2	15.27465199959038	23.427324256568564	40.08803685321797	21.209986890623085
3	15.941387830010012	27.38009352872808	33.10397977791031	23.574538863351606
4	11.286680480509116	18.416545971409878	44.058705964469084	26.23806758361192
5	10.4361990412641	39.49372866664113	38.941748157133894	11.128324134960877
6	25.443237535299946	44.019546769444574	19.39318098504525	11.144034710210232
7	23.833489787735278	33.95516467574858	26.645057460842757	15.566288075673384
8	20.956265979257353	38.90782582052583	24.138009196548676	15.997899003668145
9	22.633467589708555	15.026266362251475	25.57048537861314	36.76978066942683
10-14	20.267454957155465	26.131610849832693	32.84887442716976	20.75205976584208
15-19	19.451140396129375	32.0382216386062	28.818490206193626	19.6921477590708
20-24	19.451019663544013	30.310521604636236	30.54067945887658	19.697779272943173
25-29	19.438901255633244	30.814603539826606	30.039837472991778	19.706657731548376
30-34	19.307339410098812	30.525436195916672	30.131048331938036	20.03617606204648
35-39	19.459900837882667	30.780231511732765	30.038439318020373	19.72142833236419
40-44	19.21909131888864	30.534545166748572	30.460703369319415	19.785660145043373
45-49	19.413940669880407	30.41932436363864	30.332672232840846	19.83406273364011
50-54	19.165900898822454	30.06157164497882	30.723054291441205	20.049473164757526
55-59	19.350333491345584	29.98829061971821	30.765470043176286	19.89590584575992
60-64	18.944540299866627	29.742688801683137	30.97292810342813	20.339842795022108
65-69	19.26443442231579	29.88098509353897	30.439330298543666	20.415250185601572
70-74	19.118365483749184	29.64958013867969	30.45508230344917	20.776972074121957
75-79	19.353218968187456	29.910586084245143	30.005601168945045	20.730593778622357
80-84	19.184549523953194	29.759237011984606	29.87707432676953	21.179139137292665
85-89	19.298979812133105	29.68857842630718	29.7298805661551	21.282561195404615
90-94	19.087936863372494	29.20994485675168	30.223046107159306	21.47907217271652
95-99	19.277603785129553	29.204720719132094	29.781366434770266	21.736309060968086
100-101	19.093781191549432	28.334491434610005	30.704404354252368	21.867323019588195
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.000277475334
2	36.48463889021346
3	39.51592669336162
4	37.524748064121034
5	21.564523176224977
6	36.58727224551018
7	39.399777863408666
8	36.9541649829255
9	59.40324825913539
10-14	41.01951472299755
15-19	39.143288155200175
20-24	39.14879893648719
25-29	39.145558987181616
30-34	39.34351547214529
35-39	39.18132917024686
40-44	39.00475146393202
45-49	39.248003403520514
50-54	39.21537406357998
55-59	39.24623933710551
60-64	39.28438309488874
65-69	39.679684607917366
70-74	39.895337557871144
75-79	40.08381274680981
80-84	40.36368866124585
85-89	40.581541007537716
90-94	40.56700903608901
95-99	41.01391284609764
100-101	40.96110421113763
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.0
6	3.0
7	4.0
8	8.0
9	14.0
10	19.5
11	31.0
12	59.0
13	99.0
14	158.0
15	229.5
16	351.0
17	558.0
18	816.5
19	1201.5
20	1809.0
21	2704.5
22	3851.5
23	5335.0
24	7140.5
25	9330.0
26	11972.0
27	14890.5
28	18484.0
29	22457.5
30	26558.0
31	31070.0
32	35828.5
33	41110.5
34	47228.5
35	54173.5
36	62554.5
37	70059.5
38	74025.5
39	74225.5
40	70974.5
41	66325.5
42	60974.5
43	55331.5
44	50839.0
45	46581.0
46	42493.0
47	38714.0
48	34196.5
49	29361.5
50	24912.0
51	21724.0
52	19360.5
53	17097.0
54	14891.5
55	12569.5
56	10740.5
57	9274.5
58	7685.5
59	6294.5
60	5024.5
61	4021.0
62	3140.0
63	2158.0
64	1457.0
65	1062.5
66	786.0
67	522.0
68	367.0
69	396.0
70	414.5
71	336.5
72	292.5
73	260.0
74	188.0
75	126.0
76	69.0
77	36.5
78	16.5
79	4.5
80	4.5
81	5.0
82	2.0
83	0.5
84	0.0
85	0.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.01219328228308268
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0010161068569235568
20-24	7.97253072355406E-4
25-29	0.019196603389263503
30-34	0.007347234196216488
35-39	0.04675654783166705
40-44	0.05576081782532811
45-49	0.056042201262630015
50-54	0.05627668746038161
55-59	0.046053089238412274
60-64	0.057152102598654206
65-69	0.04520893892650655
70-74	0.0032828067685222607
75-79	0.0013443875337757827
80-84	1.5632413183439334E-4
85-89	0.0031577474630547454
90-94	0.002673142654368126
95-99	0.0022354350852318247
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1279393.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	27.782569293341446
#Duplication Level	Relative count
1	100.0
2	7.241412757496029
3	2.983255938019084
4	2.066636759338967
5	1.5452792899838743
6	1.2209464456757642
7	0.9323811486838754
8	0.7299004570971301
9	0.5959237120651818
10++	3.93018659747566
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AGAAG	343285	2.7530246	5.074147	5
CCGAG	155890	2.6573308	5.9343615	95-97
GAGCC	153895	2.6233234	5.997727	95-97
CTCCG	160920	2.5722265	5.8075967	95-97
CGAGC	142480	2.4287412	5.689162	95-97
GCCCA	143020	2.31758	5.27697	90-94
AGCCC	141545	2.293678	5.243278	95-97
ACACA	306920	2.2243423	7.5440707	6
GACTC	193965	2.1266036	5.214702	7
CTTTG	288360	2.110011	8.1206875	9
CCACG	121735	1.9726653	5.0585365	90-94
TACAC	271065	1.9378101	9.465787	5
CACAT	265245	1.8962036	5.1921453	7
AGAGA	234255	1.8786426	5.074147	8
GAGAG	146835	1.8059689	5.98107	7
TGGAG	143370	1.7394031	5.1998587	5
TGGCT	140455	1.5979056	5.1297255	6
TTGAG	198465	1.5486804	5.569074	9
AGACT	195725	1.4718835	5.3670077	6
GAGTC	127150	1.4664557	7.006497	9
CCATG	127465	1.3975074	6.0067415	9
GTCTT	189205	1.3844663	6.102619	1
AAGAC	181145	1.3809953	5.8031363	5
TATAC	276680	1.3382608	6.008131	5
TATGA	253100	1.2877885	6.031545	4
GATTG	161905	1.2633921	6.1781917	7
GACTT	170240	1.262845	5.963075	7
GTGTT	163595	1.2592419	5.6021905	1
TCATA	260230	1.2586945	6.1494136	2
GTTCT	165885	1.2138273	5.456877	1
AACAC	163345	1.1838107	5.003613	5
GTATG	149715	1.1682699	5.9209247	3
GTGTA	149225	1.1644462	10.847847	1
TGGAC	99530	1.1479067	7.2413516	5
ACATG	152100	1.1438165	5.600356	8
ACCAT	158275	1.1314883	5.1366887	8
GTCCA	101540	1.1132696	7.474575	1
ACACC	102455	1.0825441	5.5070395	6
GGACT	91205	1.0518922	7.0232725	6
ACACT	142290	1.0172136	5.039639	6
GAGTA	125470	0.9925592	5.193657	1
CATAC	128710	0.92013186	5.1990776	3
GTATA	150345	0.7649647	7.033762	1
ATACT	156670	0.75778997	5.0044312	6
>>END_MODULE