##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-122_TCCTGAGC-CTCTCTAT_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1329196	
Filtered Sequences	0	
Sequence length	101	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.614445123217344	33.0	31.0	34.0	30.0	34.0
2	31.815804441180983	33.0	31.0	34.0	30.0	34.0
3	31.956367608689764	34.0	31.0	34.0	30.0	34.0
4	35.480530335631464	37.0	35.0	37.0	33.0	37.0
5	35.35849340503582	37.0	35.0	37.0	33.0	37.0
6	35.502162209335566	37.0	35.0	37.0	33.0	37.0
7	35.51068691148634	37.0	35.0	37.0	33.0	37.0
8	35.55942238766894	37.0	35.0	37.0	33.0	37.0
9	37.352083515147505	39.0	37.0	39.0	34.0	39.0
10-14	37.47070996301524	39.2	37.2	39.4	34.0	39.4
15-19	38.523218246218015	40.0	38.0	41.0	34.0	41.0
20-24	38.42419221845386	40.0	38.0	41.0	34.0	41.0
25-29	38.24662382372502	40.0	38.0	41.0	33.4	41.0
30-34	37.984728963975215	40.0	38.0	41.0	33.0	41.0
35-39	37.683466998095085	40.0	37.6	41.0	31.8	41.0
40-44	37.64998013836936	40.0	37.6	41.0	31.8	41.0
45-49	37.49993063475965	40.0	37.0	41.0	31.4	41.0
50-54	36.829445017890514	39.2	36.4	40.2	31.0	40.6
55-59	37.47531440058501	40.0	36.6	41.0	32.0	41.0
60-64	37.034038922777384	39.2	35.6	41.0	31.0	41.0
65-69	36.37419176705316	38.2	35.0	40.2	30.4	41.0
70-74	35.54234529745802	36.6	34.6	39.4	29.6	41.0
75-79	34.58046879466986	35.6	34.0	38.0	29.0	39.6
80-84	33.64495845608924	35.0	33.8	36.4	28.0	38.2
85-89	32.86245549941468	35.0	33.0	35.6	26.2	36.8
90-94	32.27153753095856	35.0	32.6	35.0	25.4	36.0
95-99	31.78943571903617	35.0	32.0	35.0	24.4	35.2
100-101	30.926517609141165	34.5	30.5	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	3.0
6	42.0
7	123.0
8	269.0
9	419.0
10	459.0
11	636.0
12	982.0
13	1441.0
14	1788.0
15	2086.0
16	2446.0
17	2838.0
18	3155.0
19	3592.0
20	4104.0
21	4704.0
22	5439.0
23	6111.0
24	7053.0
25	8046.0
26	9398.0
27	11362.0
28	13609.0
29	16309.0
30	19908.0
31	24980.0
32	33441.0
33	44419.0
34	63536.0
35	96670.0
36	167640.0
37	310870.0
38	381943.0
39	79323.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.243181489776745	23.817447559184064	10.785965007345327	24.153405943693855
2	14.937668152998063	23.69889559933021	40.41406558672461	20.949370660947114
3	15.591027240668803	27.88989044431835	32.93744384123683	23.58163847377602
4	10.937668144899419	18.63920309382852	44.591933259032885	25.831195502239172
5	10.453845893336243	39.53159160266566	38.908752930374675	11.105809573623429
6	25.1997484148026	44.04245650675759	19.623043518822243	11.134751559617566
7	23.7470940948999	34.0335843634093	26.88208608250137	15.33723545918943
8	20.865317988222575	38.835634422824164	24.44564320285174	15.853404386101525
9	22.693311485424573	15.212960685049303	25.680170389613515	36.41355743991261
10-14	20.186382323022567	26.251376875711006	33.07404431282616	20.488196488440263
15-19	19.425146903206805	32.098277315989655	28.953179856361977	19.523395924441566
20-24	19.375236564455772	30.394162473408837	30.678679437949192	19.5519215241862
25-29	19.365027966383764	30.970460300254178	30.134372861442866	19.5301388719192
30-34	19.244945228216892	30.66705247139982	30.21841152274149	19.8695907776418
35-39	19.424499295547555	30.92191856039203	30.089832546018886	19.56374959804153
40-44	19.08886847605197	30.69643054926851	30.556732704735673	19.65796826994385
45-49	19.321616176694537	30.51019259603468	30.494828612582637	19.67336261468815
50-54	19.053142161092985	30.1568616534237	30.87162834720077	19.91836783828255
55-59	19.295836468805756	30.138123296932513	30.880720534948086	19.68531969931365
60-64	18.94361886165081	29.740874414169955	31.211273218011243	20.104233506167997
65-69	19.34259330184007	29.950960260610245	30.62052945221075	20.085916985338944
70-74	19.277609056185796	29.74783061489238	30.649280483510267	20.325279845411558
75-79	19.583527638246913	30.17452606529879	30.18614297862172	20.05580331783258
80-84	19.69207830964051	30.094498921863266	29.972863368346253	20.24055940014997
85-89	19.960399603454306	30.132461791546255	29.7494141801803	20.157724424819136
90-94	19.972230026393063	29.758625147039858	30.169772442416882	20.0993723841502
95-99	20.445708087597076	29.61340103796106	29.609097267617468	20.331793606824395
100-101	20.563425636295307	28.99675311362146	30.326054203553554	20.113767046529677
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.39658743347061
2	35.887038813945175
3	39.172665714444825
4	36.768863647138595
5	21.559655466959672
6	36.33449997442016
7	39.08432955408933
8	36.718722374324095
9	59.106868925337174
10-14	40.67457881146283
15-19	38.94854282764837
20-24	38.927158088641974
25-29	38.89516683830296
30-34	39.114536005858696
35-39	38.98824889358908
40-44	38.74683674599582
45-49	38.994978791382685
50-54	38.97150999937553
55-59	38.981156168119405
60-64	39.047852367818805
65-69	39.428510287179016
70-74	39.60288890159735
75-79	39.63933095607949
80-84	39.932637709790484
85-89	40.11812402827344
90-94	40.071602410543264
95-99	40.77750169442147
100-101	40.67719268282498
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	2.0
2	4.0
3	7.5
4	9.5
5	14.5
6	20.0
7	30.0
8	39.5
9	52.5
10	71.5
11	83.5
12	117.0
13	169.5
14	220.0
15	313.5
16	441.0
17	623.0
18	931.5
19	1366.5
20	2017.5
21	2958.0
22	4227.0
23	5902.0
24	7833.5
25	10171.0
26	13081.5
27	16334.5
28	20147.0
29	24284.0
30	28707.5
31	33442.0
32	38118.0
33	43396.5
34	49602.0
35	56976.5
36	65681.0
37	73697.0
38	78371.5
39	78766.0
40	75544.0
41	70511.0
42	64180.5
43	57965.5
44	53220.0
45	48142.5
46	43056.0
47	38342.5
48	33530.0
49	28710.5
50	24426.0
51	21145.5
52	18369.5
53	16121.5
54	13798.0
55	11720.5
56	10288.5
57	8764.0
58	7134.5
59	5770.0
60	4647.0
61	3733.5
62	2881.0
63	2037.5
64	1410.0
65	1021.0
66	790.0
67	603.0
68	456.5
69	441.0
70	466.5
71	379.0
72	313.0
73	259.5
74	200.5
75	151.0
76	92.0
77	72.0
78	51.0
79	30.5
80	29.0
81	21.0
82	18.5
83	18.0
84	11.5
85	10.0
86	9.0
87	6.0
88	6.5
89	11.0
90	9.5
91	5.0
92	4.0
93	5.5
94	7.5
95	5.0
96	2.0
97	2.5
98	3.0
99	1.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03460738672099525
2	0.031673282194649995
3	0.028137310073157007
4	0.026858341433468054
5	0.03317795118251936
6	0.0018056027854432303
7	4.514006963608076E-4
8	0.003235038323919121
9	5.266341457542755E-4
10-14	0.007523344939346793
15-19	0.02466152471117879
20-24	0.020839665481990614
25-29	0.0021667233425318763
30-34	0.00197111637410886
35-39	0.0059133491223265795
40-44	0.013903141447912874
45-49	0.008546519851097957
50-54	0.00473970731178848
55-59	0.0037767191595520903
60-64	0.01065305643411506
65-69	0.014459868973424538
70-74	0.018657895449580048
75-79	0.007478204869710713
80-84	0.010818570022780688
85-89	0.008907640408186602
90-94	0.010126422288360783
95-99	0.00969006828187867
100-101	0.005755358878600297
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1329196.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	26.325719828330737
#Duplication Level	Relative count
1	100.0
2	7.028807771846294
3	2.8976097725195977
4	1.9453662290193816
5	1.44519790314048
6	1.1187659308421103
7	0.9179772038666859
8	0.7207954600105805
9	0.5644928581734238
10++	3.593757514548165
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GCTGC	168065	2.7946467	6.2727466	90-94
ACACA	339500	2.4277492	7.4731183	6
GACGC	142190	2.3854074	6.0051184	95-97
CTGCC	145655	2.378801	6.008773	90-94
CGCTG	142585	2.370956	5.9178987	90-94
GAGAG	205460	2.3328662	5.63849	7
GCCGA	138955	2.3311365	6.2780666	90-94
CGACG	133825	2.2450747	6.205832	95-97
CTTTG	308230	2.1853657	8.953553	9
CCGAC	131010	2.1586442	5.8926706	95-97
TACAC	302100	2.141267	9.643507	5
TGCCG	127840	2.1257706	5.9566092	90-94
CACAT	292265	2.0715568	5.283166	7
GCTTT	258390	1.8319979	5.4027796	1
ATACA	364220	1.7160739	5.0038795	6
GCCAA	154590	1.6932074	5.153382	1
CACCA	149570	1.6090012	5.164727	7
TTGAG	209365	1.524804	5.848918	9
GACTC	140365	1.5238551	5.4649043	7
GAGTC	133585	1.4765884	7.8692446	9
TATAC	308040	1.4385848	5.7242107	5
CCATG	129975	1.4110574	6.0493693	9
AAGAC	193115	1.4060376	5.809367	5
GTGTA	188300	1.3713877	11.071355	1
GTCTT	188700	1.3378923	6.4138665	1
GACTT	183660	1.3137348	6.95864	7
TATGA	272340	1.294961	6.425522	4
ACTCA	182385	1.292734	5.0701475	8
TGAGT	177110	1.2898912	5.4957643	8
TCATA	275465	1.2864555	6.3222284	2
GTGTT	176740	1.2758539	6.0716534	1
ATGAG	171250	1.2583008	5.163244	7
GATTG	171975	1.252493	6.5552354	7
GTTCT	172780	1.2250185	5.5506587	1
TGGAC	105465	1.1657625	8.138709	5
ACCAT	164215	1.1639462	5.321077	8
ACACC	107960	1.1613811	5.467184	6
GTCCA	104805	1.1378024	8.446583	1
ACATG	156990	1.1329454	5.5717106	8
GGACT	98470	1.0884429	7.9333024	6
ACACT	153000	1.084455	5.2727356	6
AGACT	140260	1.0122105	5.5924783	6
GTATG	136780	0.996168	6.008919	3
TGTAT	208300	0.98172665	5.118438	2
GAGTA	132615	0.97442085	5.146511	1
CATAC	134995	0.95683664	5.3563576	3
GTATA	158785	0.75501347	7.274427	1
>>END_MODULE