##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-122_TCCTGAGC-CTCTCTAT_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1329196	
Filtered Sequences	0	
Sequence length	101	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.48721106593761	34.0	31.0	34.0	31.0	34.0
2	32.66825058155457	34.0	31.0	34.0	31.0	34.0
3	32.77752791913307	34.0	31.0	34.0	31.0	34.0
4	36.27400849837044	37.0	37.0	37.0	35.0	37.0
5	36.01397536555933	37.0	35.0	37.0	35.0	37.0
6	36.10341213786379	37.0	36.0	37.0	35.0	37.0
7	36.10178709535689	37.0	36.0	37.0	35.0	37.0
8	36.12614241992904	37.0	36.0	37.0	35.0	37.0
9	37.990408487536826	39.0	38.0	39.0	35.0	39.0
10-14	38.17927664543077	39.4	38.2	39.4	35.2	39.4
15-19	39.32285441725675	40.8	39.0	41.0	36.0	41.0
20-24	39.26046798214861	40.0	39.0	41.0	36.0	41.0
25-29	39.07782840152995	40.0	39.0	41.0	36.0	41.0
30-34	38.88932873707113	40.0	38.2	41.0	35.2	41.0
35-39	38.672912046078984	40.0	38.0	41.0	35.0	41.0
40-44	38.45224301006022	40.0	38.0	41.0	34.0	41.0
45-49	38.28554434409974	40.0	38.0	41.0	33.8	41.0
50-54	38.242371629165305	40.0	37.8	41.0	33.8	41.0
55-59	37.87954492791131	40.0	36.8	41.0	33.0	41.0
60-64	37.33312212796308	39.0	35.8	40.8	32.4	41.0
65-69	36.61207090602138	38.0	35.0	40.0	31.0	41.0
70-74	36.03215567907216	36.8	35.0	39.2	31.0	41.0
75-79	34.83940111164945	35.2	34.0	37.4	30.4	39.2
80-84	34.61832310659978	35.0	34.0	36.6	31.0	38.4
85-89	33.98381713456857	35.0	34.0	35.8	30.8	36.8
90-94	33.572122997661744	35.0	34.0	35.0	30.0	36.0
95-99	33.29651127448473	35.0	34.0	35.0	29.4	35.6
100-101	32.95933443976659	35.0	33.5	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	2.0
12	1.0
13	2.0
14	14.0
15	41.0
16	86.0
17	210.0
18	378.0
19	617.0
20	936.0
21	1329.0
22	1845.0
23	2691.0
24	3736.0
25	4692.0
26	6213.0
27	7846.0
28	10322.0
29	13031.0
30	16402.0
31	21033.0
32	27760.0
33	37149.0
34	53257.0
35	85623.0
36	159724.0
37	323942.0
38	449911.0
39	100317.0
40	86.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.73153996852233	23.91806776427254	11.180442914363269	24.16994935284187
2	15.09983386104523	23.46885797527178	40.34174361629125	21.08956454739174
3	15.749144595680395	27.447043174971935	33.175618945588155	23.628193283759504
4	11.159377548533097	18.569571379992116	44.30550498195902	25.96554608951577
5	10.290431208038543	39.553760318267585	39.12018994941303	11.035618524280844
6	25.184547651362177	44.23621497506763	19.50359465421202	11.075642719358168
7	23.701320196569956	34.05291619896539	26.845777447419344	15.399986157045312
8	20.812731907107757	38.94196190779991	24.34103021676261	15.904275968329726
9	22.453498204929897	15.092055648677846	25.735482201270543	36.718963945121715
10-14	20.118733429832773	26.22641055194268	33.06428848717571	20.590567531048844
15-19	19.335524857630418	32.15275206228435	28.94732050869806	19.56440257138717
20-24	19.306445322374454	30.370981604397034	30.749468547394983	19.573104525833532
25-29	19.3379537145198	30.95993505862211	30.123215850319397	19.578895376538693
30-34	19.156260556900236	30.648638521954968	30.24489375197031	19.95020716917449
35-39	19.36800197256651	30.9204015822681	30.116893833953373	19.594702611212018
40-44	19.105668230189362	30.61322436999801	30.576912625542363	19.70419477427027
45-49	19.27392190744767	30.482703156948983	30.495936273885476	19.747438661717872
50-54	19.0156463824233	30.102989418029246	30.898059896754653	19.983304302792803
55-59	19.227133917562536	30.088049844885777	30.872163571331697	19.812652666219993
60-64	18.806842295191593	29.721330767791297	31.113871495971857	20.35795544104525
65-69	19.161120036031523	29.91322388196304	30.515535583537257	20.410120498468178
70-74	18.972120640873605	29.64093845968418	30.53051570923393	20.856425190208284
75-79	19.196727359835133	29.962798048958994	30.072986484274182	20.767488106931683
80-84	19.03049230879882	29.70207062293832	29.968231880638662	21.2992051876242
85-89	19.150692846728067	29.715608575078583	29.751435875752545	21.3822627024408
90-94	18.955928622696106	29.13605715480882	30.27701664657882	21.630997575916254
95-99	19.157914440522354	29.09397512697054	29.862351341058375	21.88575909144873
100-101	19.009964677868936	28.230620789274713	30.778967721064255	21.980446811792103
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	64.9014893213642
2	36.189398408436965
3	39.3773378794399
4	37.12492363804887
5	21.326049732319387
6	36.260190370720345
7	39.101306353615264
8	36.71700787543749
9	59.17246215005161
10-14	40.70930096088162
15-19	38.89992742901759
20-24	38.879549848207986
25-29	38.916849091058495
30-34	39.106467726074726
35-39	38.96270458377853
40-44	38.80986300445963
45-49	39.021360569165545
50-54	38.9989506852161
55-59	39.03978658378253
60-64	39.16479773623684
65-69	39.5712405344997
70-74	39.82854583108189
75-79	39.96421546676682
80-84	40.32969749642302
85-89	40.53295554916887
90-94	40.58692619861236
95-99	41.04367353197109
100-101	40.990411489661035
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.5
6	5.5
7	9.0
8	12.5
9	15.5
10	28.5
11	48.5
12	78.0
13	117.0
14	171.5
15	267.5
16	405.5
17	632.0
18	911.0
19	1344.5
20	1976.0
21	2882.5
22	4208.0
23	5911.0
24	7851.0
25	10162.5
26	13100.0
27	16458.5
28	20101.5
29	24046.5
30	28332.0
31	32882.0
32	37660.0
33	42983.0
34	49063.0
35	56159.5
36	64845.5
37	72672.5
38	77225.0
39	77596.5
40	74399.5
41	69384.0
42	63023.0
43	56984.5
44	52622.5
45	48386.5
46	44017.0
47	39800.0
48	35131.0
49	30145.5
50	25766.0
51	22560.0
52	19556.0
53	16924.5
54	14591.0
55	12489.5
56	10882.5
57	9175.5
58	7321.0
59	5963.5
60	4785.0
61	3814.0
62	3000.5
63	2014.5
64	1317.5
65	968.0
66	725.0
67	523.0
68	384.5
69	395.5
70	449.5
71	376.5
72	300.5
73	255.0
74	195.0
75	154.5
76	106.5
77	64.5
78	35.0
79	17.5
80	6.5
81	7.0
82	5.5
83	3.5
84	2.5
85	0.5
86	0.5
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.01414388848597197
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0010984083611446317
20-24	6.620543546625178E-4
25-29	0.01832686827224879
30-34	0.006695776996018646
35-39	0.04300343967330627
40-44	0.052678461265306245
45-49	0.053475935828877004
50-54	0.05341574906936223
55-59	0.04229624524900767
60-64	0.054002569974631286
65-69	0.04375577416724095
70-74	0.003596158881007767
75-79	0.00138429546883981
80-84	1.3542020890824227E-4
85-89	0.0031598048745256533
90-94	0.0025579372793779094
95-99	0.002211863412167957
100-101	7.523344939346793E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1329196.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	27.090563179311935
#Duplication Level	Relative count
1	100.0
2	7.319062058301219
3	2.8443891419114005
4	2.0126363603416406
5	1.4835038718484603
6	1.171823090955217
7	0.8879278060330769
8	0.7085301472631287
9	0.5883276755620522
10++	3.7903040699711275
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	172460	2.8775933	6.386697	85-89
GAGCC	169930	2.835379	6.3697743	95-97
CTCCG	179755	2.7884862	6.2228947	95-97
AGAAG	356235	2.7783601	5.089736	5
CGAGC	160160	2.672361	6.24842	95-97
GCCCA	156610	2.4702861	5.9075065	90-94
AGCCC	155705	2.456011	5.6933556	90-94
ACACA	341405	2.3795521	7.775713	6
CAAAG	316630	2.3344831	5.0331464	4
CCCAC	151995	2.2664375	5.616398	90-94
GACTC	212890	2.2578664	5.851719	7
CATCT	328840	2.2168155	5.028283	95-97
CTTTG	309400	2.1698925	8.820356	9
CCACG	135140	2.131629	5.7162995	90-94
TACAC	302960	2.0766838	10.066595	5
CACAT	293850	2.0142379	5.440415	7
GCTTT	259015	1.8165312	5.1862254	1
GAGAG	148735	1.7948287	5.944315	7
TGGAG	148005	1.7564912	5.253377	5
TGGCT	150920	1.6651862	5.2104254	6
TTGAG	209825	1.5828097	5.7530537	9
AGACT	214280	1.5537461	5.5264874	6
CACCA	149690	1.5260286	5.081141	7
GAGTC	134665	1.5108141	7.72945	9
ATGGA	196955	1.5107018	5.0650887	4
TATAC	309175	1.4249666	5.9060307	5
AAGAC	191975	1.4154135	6.0983996	5
GTCTT	201710	1.4146382	6.441122	1
CATGA	194710	1.4118439	5.1081343	9
CCATG	130425	1.3832599	6.09854	9
TGAGT	178490	1.3464351	5.460464	8
TATGA	271285	1.322633	6.387004	4
ATGAG	172030	1.3195199	5.087402	7
GTGTT	175800	1.3042177	6.029327	1
GACTT	182830	1.3037839	6.831929	7
GATTG	170990	1.2898588	6.312632	7
TCATA	278340	1.2828503	6.53717	2
ACTCA	182990	1.254332	5.0083723	8
GTATG	162910	1.2289076	5.9542093	3
GTGTA	161860	1.220987	11.474918	1
GTTCT	172285	1.2082739	5.0535936	1
TGGAC	104980	1.1777765	7.9796643	5
ACATG	160100	1.1608865	5.776094	8
GTCCA	106625	1.1308422	8.053753	1
ACCAT	164625	1.1284462	5.24101	8
ACACC	109705	1.1183978	5.5853014	6
GGACT	97755	1.0967188	7.7457685	6
ACACT	154815	1.0612023	5.2144237	6
TGTAT	212790	1.0202924	5.1942177	2
GAGTA	132370	1.0153161	5.2890162	1
CATAC	135750	0.93051845	5.5799985	3
GTATA	160090	0.78050894	7.0948505	1
>>END_MODULE