##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-120_AGGCAGAA-CTAAGCCT_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1168013	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.55376609678146	31.0	31.0	34.0	30.0	34.0
2	31.749501075758573	33.0	31.0	34.0	30.0	34.0
3	31.89140189364331	34.0	31.0	34.0	30.0	34.0
4	35.419769300512925	37.0	35.0	37.0	33.0	37.0
5	35.28937434771702	37.0	35.0	37.0	33.0	37.0
6	35.43547717362735	37.0	35.0	37.0	33.0	37.0
7	35.44868764303137	37.0	35.0	37.0	33.0	37.0
8	35.49955094677885	37.0	35.0	37.0	33.0	37.0
9	37.27934106897783	39.0	37.0	39.0	34.0	39.0
10-14	37.39391273898492	39.2	37.2	39.4	33.2	39.4
15-19	38.426636347369424	40.0	38.0	41.0	33.8	41.0
20-24	38.31904542158348	40.0	38.0	41.0	33.8	41.0
25-29	38.131788087975046	40.0	38.0	41.0	33.0	41.0
30-34	37.86021576814642	40.0	38.0	41.0	32.4	41.0
35-39	37.540575832632	40.0	37.2	41.0	31.6	41.0
40-44	37.48708790056275	40.0	37.0	41.0	31.6	41.0
45-49	37.325125148435845	40.0	36.8	41.0	31.0	41.0
50-54	36.65238160876633	38.8	35.6	40.2	30.6	40.6
55-59	37.28606702151431	39.8	36.2	41.0	31.0	41.0
60-64	36.83579429338543	39.0	35.2	41.0	31.0	41.0
65-69	36.16890890769196	38.0	35.0	40.2	30.0	41.0
70-74	35.343822714301986	36.6	34.4	39.2	29.0	41.0
75-79	34.39764506045738	35.4	34.0	37.6	28.6	39.4
80-84	33.48756871712901	35.0	33.4	36.4	27.4	38.2
85-89	32.724530292042985	35.0	33.0	35.4	26.0	36.6
90-94	32.15910199629627	35.0	32.2	35.0	25.2	36.0
95-99	31.68076999142989	34.8	31.8	35.0	24.0	35.2
100-101	30.82438037932797	34.0	30.0	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	3.0
6	27.0
7	144.0
8	262.0
9	356.0
10	427.0
11	587.0
12	920.0
13	1294.0
14	1682.0
15	1968.0
16	2197.0
17	2563.0
18	2875.0
19	3424.0
20	3815.0
21	4311.0
22	5005.0
23	5819.0
24	6590.0
25	7633.0
26	8905.0
27	10571.0
28	12843.0
29	15534.0
30	18836.0
31	23801.0
32	30924.0
33	41351.0
34	58231.0
35	88609.0
36	151337.0
37	271955.0
38	320503.0
39	62663.0
40	48.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.870722199003815	23.481573340093316	10.676175174880273	23.971529286022598
2	15.060122823379091	23.428760812987658	40.02541763973922	21.485698723894036
3	15.868376544134962	27.675094735489946	32.701791945877666	23.75473677449742
4	11.158943091171446	18.52399282377574	44.2201850575254	26.096879027527415
5	10.685871667114847	39.286723611394194	38.78359005277122	11.243814668719738
6	25.493645937946546	43.74974850020506	19.478455778416482	11.278149783431921
7	24.036219054044334	33.86264088136684	26.547340591299346	15.553799473289477
8	21.234535133607885	38.47333407536153	24.270058306292114	16.022072484738477
9	22.735499646405927	15.245127165442643	25.541135918822334	36.47823726932909
10-14	20.38505251408855	26.127002702720752	32.79575167847641	20.692193104714292
15-19	19.63278836774943	31.9058951682725	28.733607999869832	19.727708464108233
20-24	19.557984347241398	30.20677212934138	30.493438511705147	19.74180501171208
25-29	19.574066885379295	30.747349583411175	29.88603803709325	19.792545494116283
30-34	19.449552469640523	30.452544110477863	30.021238235536362	20.076665184345245
35-39	19.571665348398636	30.740908873744743	29.95371534965725	19.733710428199373
40-44	19.304311609597143	30.507163467557053	30.36622145774454	19.822303465101268
45-49	19.503701798446947	30.333684156809532	30.301935374274308	19.860678670469216
50-54	19.238507976203074	29.958032421273117	30.733785339293867	20.069674263229942
55-59	19.513889542922946	29.90704344736358	30.743027402362227	19.836039607351246
60-64	19.121619781852857	29.57081767761467	31.050356942679393	20.257205597853087
65-69	19.480615561667484	29.769763769818567	30.478094677028782	20.271525991485166
70-74	19.48271787195709	29.480525708257264	30.50431753491192	20.532438884873727
75-79	19.825227456037155	29.73631710445425	30.077043080079253	20.361412359429345
80-84	19.86121110283777	29.542817275556345	29.979114192416585	20.616857429189302
85-89	20.089922082370066	29.513365870365615	29.810377600821987	20.586334446442333
90-94	20.119460371618718	29.075272124349212	30.225213916573306	20.580053587458767
95-99	20.51347872227292	28.95028190092137	29.732494237911943	20.803745138893763
100-101	20.783763732112025	28.239399929019626	30.342739302962467	20.634097035905878
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.84225148502641
2	36.54582154727313
3	39.623113318632385
4	37.25582211869886
5	21.929686335834585
6	36.77179572137847
7	39.59001852733381
8	37.25660761834636
9	59.213736915735026
10-14	41.077245618802834
15-19	39.36049683185767
20-24	39.29978935895348
25-29	39.36661237949558
30-34	39.52621765398577
35-39	39.305375776598005
40-44	39.12661507469841
45-49	39.36438046891616
50-54	39.308182239433016
55-59	39.34992915027419
60-64	39.37882537970594
65-69	39.75214155315265
70-74	40.01515675683082
75-79	40.1866398154665
80-84	40.47806853202707
85-89	40.6762565288124
90-94	40.699513959077485
95-99	41.31722386116669
100-101	41.4178607680179
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	3.0
2	3.5
3	4.5
4	9.0
5	13.0
6	21.0
7	28.0
8	25.5
9	31.5
10	47.5
11	63.0
12	94.5
13	140.5
14	199.0
15	269.5
16	384.0
17	555.0
18	794.5
19	1155.5
20	1670.5
21	2398.5
22	3469.0
23	4889.0
24	6596.5
25	8675.5
26	11097.5
27	13729.0
28	16846.0
29	20327.0
30	23933.0
31	27947.0
32	31883.5
33	36333.5
34	42059.5
35	48509.0
36	56157.0
37	63352.5
38	67624.0
39	68089.5
40	65380.5
41	60848.5
42	55039.5
43	49861.0
44	46217.0
45	42499.5
46	38542.0
47	34845.0
48	30772.0
49	26290.5
50	22653.0
51	20430.0
52	18181.5
53	15843.0
54	13650.5
55	11647.0
56	10293.5
57	8835.5
58	7260.0
59	5921.0
60	4852.0
61	4085.0
62	3262.5
63	2309.0
64	1652.5
65	1332.0
66	1047.5
67	788.5
68	603.0
69	614.0
70	640.5
71	537.5
72	452.0
73	372.0
74	280.0
75	206.5
76	140.0
77	106.5
78	73.5
79	44.5
80	32.0
81	16.5
82	16.0
83	17.0
84	14.0
85	13.0
86	8.5
87	7.0
88	9.0
89	7.0
90	6.0
91	6.0
92	4.5
93	3.0
94	3.0
95	4.0
96	3.0
97	2.0
98	1.0
99	0.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03056472830353772
2	0.02739695534210664
3	0.024657259807895977
4	0.02294495009901431
5	0.028338725681991556
6	0.001712309708881665
7	7.705393689967493E-4
8	0.0036814658740955794
9	5.993083981085827E-4
10-14	0.0067465002529937595
15-19	0.025170952720560472
20-24	0.020924424642533943
25-29	0.002294495009901431
30-34	0.0021575102331908977
35-39	0.005684868233487127
40-44	0.013424508117632251
45-49	0.008064978728832642
50-54	0.005462267971332511
55-59	0.004109543301315996
60-64	0.010479335418355789
65-69	0.014469017040050068
70-74	0.018578560341366065
75-79	0.008133471117187908
80-84	0.011866306282549937
85-89	0.009674549855181406
90-94	0.010941659039753839
95-99	0.010308104447467622
100-101	0.007234508520025034
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1168013.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	24.92883609946567
#Duplication Level	Relative count
1	100.0
2	6.529815539934896
3	2.739184790300514
4	1.8310374109543803
5	1.2584327970939284
6	0.935273859508421
7	0.7465679105533802
8	0.5767325564938435
9	0.4452280983157994
10++	2.8789451337453413
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GTCTC	242260	2.8855095	5.1113234	90-94
AGAAG	317490	2.725161	5.0295267	5
GCTGC	148630	2.7074761	6.2109914	90-94
ACACA	298870	2.4572687	7.8506603	6
GACGC	127295	2.3611226	6.5317664	95-97
GAAGG	182990	2.3591628	5.1027884	95-97
CAAAG	276710	2.324562	5.171016	4
CGCTG	125945	2.2942417	5.967099	95-97
GCCGA	123535	2.2913802	6.19112	90-94
CTGCC	128215	2.2858677	5.8695054	90-94
CGACG	122420	2.2706988	6.2678566	95-97
CTTTG	271940	2.1639264	8.578292	9
TACAC	267470	2.1597147	9.917062	5
CCGAC	117205	2.1276848	5.9096336	90-94
CACAT	256605	2.071984	5.5408607	7
TGCCG	113605	2.0694535	5.728533	95-97
GCTTT	229635	1.8272897	5.8129425	1
ATACA	317560	1.7443138	5.058409	6
GAGAG	134665	1.7361424	5.5581703	7
GCCAA	138455	1.7098082	5.3432484	1
CACCA	132800	1.6050587	5.1051726	7
TGGCT	131625	1.601862	5.234805	6
GACTC	125405	1.5209137	5.540387	7
TTGAG	182560	1.5113666	5.7651515	9
TATAC	271725	1.4658161	5.7906766	5
GAGTC	118170	1.4643433	7.0369525	9
AAGAC	168915	1.4190066	5.7007494	5
GTGTA	170290	1.4097865	10.862586	1
CATGA	168895	1.3934264	5.097165	9
CCATG	114740	1.3915685	6.0696197	9
GTCTT	167920	1.3362008	6.511576	1
GACTT	163595	1.3255262	6.573758	7
TATGA	235510	1.298091	6.2267766	4
TGAGT	156040	1.2918144	5.211252	8
ACTCA	158310	1.2782907	5.0240173	8
AACAC	155420	1.2778422	5.407892	5
TCATA	236780	1.2773058	6.3897834	2
GTGTT	155245	1.2622137	6.069538	1
GATTG	147620	1.2221076	6.0864344	7
GTTCT	149215	1.1873584	5.2802825	1
ACACC	97365	1.176781	5.550511	6
ACCAT	143350	1.1574947	5.067092	8
ACATG	138365	1.1415464	5.7134533	8
TGGAC	91445	1.1331716	7.4413953	5
GTCCA	91430	1.1088644	7.6418877	1
ACACT	134205	1.0836525	5.638635	6
GGACT	84530	1.0474819	6.916735	6
AGACT	126180	1.0410169	5.725284	6
GAGTA	118165	0.99609804	5.237983	1
TGTAT	182010	0.9852401	5.007121	2
GTATG	117195	0.970227	5.5415134	3
CATAC	114655	0.9257938	5.3382897	3
GTATA	139490	0.76884514	7.149225	1
>>END_MODULE