##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-120_AGGCAGAA-CTAAGCCT_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1168013	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.457180699187425	34.0	31.0	34.0	31.0	34.0
2	32.643557905605505	34.0	31.0	34.0	31.0	34.0
3	32.74985980464258	34.0	31.0	34.0	31.0	34.0
4	36.25929334690624	37.0	37.0	37.0	35.0	37.0
5	35.98510033706817	37.0	35.0	37.0	35.0	37.0
6	36.078750835821175	37.0	36.0	37.0	35.0	37.0
7	36.08089293526699	37.0	36.0	37.0	35.0	37.0
8	36.102219752691106	37.0	36.0	37.0	35.0	37.0
9	37.9669275941278	39.0	38.0	39.0	35.0	39.0
10-14	38.1505878787308	39.2	38.2	39.4	35.2	39.4
15-19	39.28731084328685	40.0	39.0	41.0	36.0	41.0
20-24	39.21937255835337	40.0	39.0	41.0	36.0	41.0
25-29	39.034022395298685	40.0	39.0	41.0	36.0	41.0
30-34	38.83632870524558	40.0	38.0	41.0	35.0	41.0
35-39	38.60960554377391	40.0	38.0	41.0	34.6	41.0
40-44	38.37771446036987	40.0	38.0	41.0	34.0	41.0
45-49	38.202055627805514	40.0	38.0	41.0	33.4	41.0
50-54	38.140382170403925	40.0	37.4	41.0	33.4	41.0
55-59	37.76211857231041	39.8	36.6	41.0	33.0	41.0
60-64	37.2153445209942	39.0	35.6	40.8	32.0	41.0
65-69	36.48902212560991	37.8	35.0	40.0	31.0	41.0
70-74	35.91590761404196	36.6	35.0	39.2	31.0	41.0
75-79	34.73500603161095	35.2	33.8	37.4	30.4	39.2
80-84	34.52796432916415	35.0	34.0	36.6	31.0	38.4
85-89	33.89675046424997	35.0	34.0	35.6	30.4	36.8
90-94	33.48305917827969	35.0	34.0	35.0	30.0	36.0
95-99	33.2070600241607	35.0	34.0	35.0	29.2	35.6
100-101	32.864153053091016	35.0	33.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	1.0
13	4.0
14	12.0
15	33.0
16	103.0
17	178.0
18	346.0
19	552.0
20	880.0
21	1223.0
22	1799.0
23	2546.0
24	3314.0
25	4375.0
26	5688.0
27	7326.0
28	9307.0
29	12184.0
30	15394.0
31	19629.0
32	25445.0
33	34084.0
34	49257.0
35	78724.0
36	144856.0
37	283122.0
38	382682.0
39	84866.0
40	83.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.09449124282007	23.668486566502256	11.037205921509436	24.199816269168238
2	15.355199117696419	23.257912817720054	40.06166877309796	21.325219291485563
3	15.952048478912479	27.236854384326204	32.96161943403027	23.849477702731047
4	11.323247258378117	18.433442093538343	43.97639409835335	26.266916549730183
5	10.445003608692712	39.38123976359852	38.927049613317664	11.246707014391106
6	25.52043513214322	43.89600115752136	19.341565547643732	11.241998162691683
7	23.908381156716576	33.80698673730515	26.601159404903886	15.683472701074388
8	21.105672625219068	38.66249776329545	24.093824298188462	16.138005313297025
9	22.598635460392995	15.089814925005115	25.454254361894947	36.85729525270695
10-14	20.335355856484476	26.055189454226962	32.81696350982395	20.792491179464612
15-19	19.549942705597182	31.930122746323125	28.734444381278667	19.785490166801026
20-24	19.50895174123041	30.21747978651505	30.477324030652547	19.796244441601996
25-29	19.518258570805674	30.73477847313252	29.920007754664873	19.826955201396938
30-34	19.41694970051554	30.429480819397973	30.00647978270124	20.147089697385244
35-39	19.562330559705853	30.755368385854325	29.941455412684864	19.74084564175495
40-44	19.294229909356762	30.44639782634393	30.383627682592763	19.875744581706545
45-49	19.438747225532435	30.347278709015146	30.28916821822134	19.924805847231084
50-54	19.20290376473845	29.93022919553368	30.73271508239006	20.134151957337806
55-59	19.371574206133992	29.887106184663885	30.723206304951027	20.01811330425109
60-64	18.979064453359896	29.577759892055212	30.98569519096778	20.457480463617117
65-69	19.294709619818324	29.770069045921954	30.35641207877953	20.57880925548019
70-74	19.198828046069913	29.57842878718865	30.311071231647553	20.91167193509389
75-79	19.501253689449744	29.97082344528553	29.75369878199571	20.774224083269022
80-84	19.426913225861796	29.93052466259753	29.451865032847273	21.190697078693397
85-89	19.46217176208253	30.060256612447873	29.233215813332414	21.24435581213718
90-94	19.339330247888146	29.572293460221527	29.679487223393856	21.40888906849647
95-99	19.45718147798448	29.633817277821674	29.213537979762073	21.695463264431776
100-101	19.342309839282475	28.856124765841447	30.03303904411941	21.76852635075667
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.29430751198831
2	36.68041840918199
3	39.801526181643524
4	37.5901638081083
5	21.691710623083818
6	36.7624332948349
7	39.591853857790966
8	37.24367793851609
9	59.45593071309994
10-14	41.12784703594909
15-19	39.33543287239821
20-24	39.305196182832404
25-29	39.345213772202605
30-34	39.564039397900785
35-39	39.30317620146081
40-44	39.16997449106331
45-49	39.363553072763516
50-54	39.33705572207626
55-59	39.38968751038509
60-64	39.43654491697701
65-69	39.873518875298515
70-74	40.110499981163805
75-79	40.275477772718766
80-84	40.6176103045552
85-89	40.706527574219706
90-94	40.74821931638462
95-99	41.15264474241626
100-101	41.110836190039144
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	0.5
5	1.0
6	2.5
7	2.0
8	6.0
9	15.5
10	24.5
11	35.0
12	68.0
13	105.5
14	164.0
15	258.0
16	387.5
17	549.5
18	782.0
19	1155.5
20	1714.5
21	2477.5
22	3524.0
23	4961.0
24	6620.5
25	8534.5
26	11026.0
27	13948.5
28	16980.5
29	20179.5
30	23833.5
31	27699.0
32	31570.5
33	36247.5
34	41760.0
35	48088.5
36	55485.0
37	62400.5
38	67187.0
39	68042.0
40	65079.5
41	60597.5
42	55185.5
43	50196.5
44	46624.0
45	43063.5
46	39320.5
47	35762.5
48	31650.0
49	26860.5
50	22837.0
51	20486.5
52	18343.0
53	15900.5
54	13667.0
55	11659.5
56	10259.0
57	8903.0
58	7285.5
59	5878.5
60	4740.5
61	3980.5
62	3196.5
63	2187.0
64	1543.5
65	1272.0
66	996.5
67	731.0
68	579.0
69	587.0
70	621.5
71	511.5
72	442.0
73	386.5
74	294.5
75	212.0
76	130.5
77	84.0
78	43.5
79	25.5
80	18.5
81	12.5
82	7.5
83	4.0
84	3.0
85	2.0
86	0.5
87	0.5
88	1.0
89	1.0
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.013784093156497403
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	9.075241457072823E-4
20-24	4.794467184868662E-4
25-29	0.017105973991727832
30-34	0.006215684243240444
35-39	0.0421741881297554
40-44	0.0495713660721242
45-49	0.04981108943136763
50-54	0.050564505703275565
55-59	0.040530370809229006
60-64	0.05148915294607167
65-69	0.040342016741252026
70-74	0.004195158786760079
75-79	0.0017465559030592982
80-84	1.1986167962171655E-4
85-89	0.003561604194473863
90-94	0.0029622957963652803
95-99	0.002380110495345514
100-101	8.561548544408325E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1168013.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	25.769895470996165
#Duplication Level	Relative count
1	100.0
2	6.706161137440758
3	2.7268957345971563
4	1.8270142180094786
5	1.3293838862559242
6	0.9834123222748815
7	0.745260663507109
8	0.5995260663507109
9	0.48459715639810425
10++	3.081161137440758
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GTCTC	241080	2.8672879	5.0911865	90-94
CCGAG	154920	2.853764	6.9013877	95-97
CTCCG	161705	2.8220053	6.6961865	95-97
GAGCC	150605	2.774278	6.5947256	95-97
AGAAG	316420	2.7403495	5.0850625	5
CGAGC	143100	2.6360292	6.475633	95-97
GCCCA	142705	2.5056102	5.932381	90-94
AGCCC	141410	2.482873	5.901732	90-94
ACACA	299570	2.357045	7.805223	6
CCCAC	135980	2.2756903	5.5937247	95-97
ATCTC	287930	2.2380846	5.14842	95-97
CTTTG	270305	2.1909878	8.75611	9
CCACG	121430	2.1320643	5.748484	90-94
TACAC	271200	2.1208954	9.998845	5
CACAT	258865	2.0244308	5.361535	7
GCTTT	226805	1.8383937	5.4084644	1
GGCAG	93725	1.8113494	5.8119936	90-94
GAGAG	133145	1.7643607	5.8403187	7
ATACA	321240	1.7225621	5.0203924	6
CAGGC	93040	1.7138795	5.4021783	90-94
GCCAA	137485	1.6551806	5.026467	1
TGGCT	130525	1.6286962	5.094113	6
TTGAG	182550	1.5618664	5.666604	9
CACCA	135555	1.5554976	5.057406	7
GAGTC	119745	1.5032932	7.456457	9
GACTC	123725	1.4804971	5.5406866	7
TATAC	274165	1.4612257	6.0417104	5
GTCTT	175155	1.4197388	6.477579	1
AAGAC	169570	1.399764	5.9915295	5
CATGA	169340	1.3893939	5.1834674	9
CCATG	115020	1.3763329	6.051242	9
GACTT	164625	1.3425226	6.955075	7
TGAGT	156060	1.3352225	5.650011	8
GTGTT	155845	1.3253026	6.0701523	1
TATGA	235705	1.3179845	6.2545958	4
TCATA	238220	1.2696486	6.487071	2
ACTCA	160460	1.2548633	5.1909065	8
GATTG	146375	1.2523593	6.093881	7
GTGTA	143155	1.2248098	11.189551	1
GTTCT	148460	1.2033594	5.365228	1
GTATG	138530	1.1852388	5.666604	3
ACATG	139330	1.1431689	5.7921166	8
ACCAT	145445	1.1374397	5.164364	8
TGGAC	90295	1.1335745	7.626891	5
ACACC	97885	1.1232333	5.196499	6
GTCCA	93530	1.1191828	7.4388833	1
GGACT	84850	1.0652171	7.2434154	6
ACACT	134045	1.048287	5.255366	6
AGACT	123920	1.0167336	5.5574083	6
TGTAT	182355	1.0134896	5.0397944	2
GAGTA	117345	1.0101053	5.3471303	1
CATAC	115860	0.9060729	5.3122425	3
GTATA	138980	0.7771303	6.865543	1
>>END_MODULE