##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-119_AGGCAGAA-AAGGAGTA_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1524499	
Filtered Sequences	0	
Sequence length	101	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.624141439253158	33.0	31.0	34.0	30.0	34.0
2	31.821664691154275	33.0	31.0	34.0	30.0	34.0
3	31.964958979966532	34.0	31.0	34.0	30.0	34.0
4	35.489902584390016	37.0	35.0	37.0	33.0	37.0
5	35.3602527781258	37.0	35.0	37.0	33.0	37.0
6	35.50782125800017	37.0	35.0	37.0	33.0	37.0
7	35.523051179436656	37.0	35.0	37.0	33.0	37.0
8	35.57352349853952	37.0	35.0	37.0	33.0	37.0
9	37.36762044448701	39.0	37.0	39.0	34.0	39.0
10-14	37.489052862612574	39.2	37.2	39.4	34.0	39.4
15-19	38.533884377752955	40.0	38.0	41.0	34.0	41.0
20-24	38.43032602841983	40.0	38.0	41.0	34.0	41.0
25-29	38.25379787064472	40.0	38.0	41.0	33.6	41.0
30-34	37.99223036551681	40.0	38.0	41.0	33.0	41.0
35-39	37.68846630925963	40.0	37.6	41.0	32.0	41.0
40-44	37.64884306254054	40.0	37.6	41.0	31.8	41.0
45-49	37.49326696836141	40.0	37.0	41.0	31.6	41.0
50-54	36.822074399524034	39.2	36.4	40.2	31.0	40.6
55-59	37.46241171689847	40.0	36.6	41.0	32.0	41.0
60-64	37.022310936248566	39.0	35.6	41.0	31.0	41.0
65-69	36.35450833355745	38.2	35.0	40.2	30.4	41.0
70-74	35.515545894093734	36.6	34.6	39.4	29.6	41.0
75-79	34.55522752064777	35.4	34.0	38.0	29.0	39.6
80-84	33.639214325493164	35.0	33.8	36.4	28.0	38.2
85-89	32.87919795290125	35.0	33.0	35.4	26.4	36.8
90-94	32.31182047348014	35.0	32.6	35.0	25.4	36.0
95-99	31.83428641147026	35.0	32.0	35.0	24.6	35.2
100-101	30.974290570213558	34.0	30.5	35.0	21.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	4.0
6	38.0
7	153.0
8	302.0
9	438.0
10	511.0
11	767.0
12	1033.0
13	1469.0
14	1954.0
15	2281.0
16	2653.0
17	3014.0
18	3514.0
19	4050.0
20	4629.0
21	5315.0
22	6140.0
23	6920.0
24	8064.0
25	9278.0
26	11102.0
27	13162.0
28	15642.0
29	18920.0
30	23307.0
31	29415.0
32	37526.0
33	51080.0
34	72474.0
35	111886.0
36	194605.0
37	358852.0
38	436290.0
39	87652.0
40	59.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.50734908136483	23.593175853018373	10.800262467191601	24.099212598425197
2	15.062502337563147	23.611323451155265	40.16297654300493	21.16319766827666
3	15.739890806471827	27.641539207510352	32.90345735763734	23.715112628380474
4	11.169003124462797	18.414187501886357	44.37937880798004	26.0374305656708
5	10.582158478443167	39.253276536103904	39.0180430939792	11.146521891473721
6	25.420539045649903	43.66023121447951	19.617349482312566	11.301880257558027
7	23.917491149504983	33.99469068692436	26.664506382786122	15.423311780784537
8	21.044835842434974	38.56813932893831	24.374495719767786	16.012529108858935
9	22.78772207397612	15.229794580752145	25.469139883974467	36.51334346129727
10-14	20.285746260794564	26.19497725467846	32.90210832921041	20.617168155316573
15-19	19.505840083667845	32.03172711895767	28.84321596302658	19.61921683434791
20-24	19.46174953053525	30.308258341225	30.574027022746485	19.655965105493266
25-29	19.461844344183472	30.834574205000308	30.04785938158017	19.65572206923605
30-34	19.318068216793097	30.522663232553338	30.136338602314826	20.02292994833874
35-39	19.507865586014496	30.811404889804127	30.006911513731716	19.67381801044966
40-44	19.20153460705544	30.552050483533744	30.44100927705129	19.80540563235953
45-49	19.40172554946785	30.347537103511975	30.41060520183891	19.840132145181265
50-54	19.119858715156514	30.00043689039522	30.817316970804043	20.06238742364422
55-59	19.315483648357358	29.93512346221614	30.878227591355216	19.871165298071283
60-64	18.98059008442054	29.54484176347002	31.152614704050514	20.321953448058924
65-69	19.337796796063525	29.679927814137937	30.583445090473095	20.398830299325443
70-74	19.233333468921863	29.36531315636056	30.598377110276907	20.80297626444067
75-79	19.37202058181492	29.614239227780466	30.306208158256094	20.707532032148524
80-84	19.267871350627377	29.284096324333632	30.290488171487713	21.15754415355128
85-89	19.47414791271005	29.172634803800978	30.25246909328269	21.100748190206286
90-94	19.37540346070883	28.677632925542802	30.727515101895147	21.219448511853216
95-99	19.824118002005065	28.422566038735976	30.30147732113868	21.451838638120282
100-101	19.959891943095627	27.729499561133625	30.999742846609312	21.310865649161432
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.60656167979002
2	36.225700005839805
3	39.4550034348523
4	37.2064336901336
5	21.72868036991689
6	36.72241930320793
7	39.34080293028952
8	37.057364951293906
9	59.30106553527339
10-14	40.90291441611114
15-19	39.12505691801575
20-24	39.117714636028516
25-29	39.11756641341952
30-34	39.340998165131836
35-39	39.18168359646416
40-44	39.00694023941497
45-49	39.24185769464912
50-54	39.182246138800735
55-59	39.18664894642864
60-64	39.30254353247947
65-69	39.73662709538897
70-74	40.036309733362536
75-79	40.07955261396344
80-84	40.42541550417866
85-89	40.574896102916334
90-94	40.59485197256205
95-99	41.27595664012535
100-101	41.270757592257056
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	4.0
2	5.5
3	7.0
4	10.5
5	14.0
6	21.0
7	28.5
8	36.0
9	47.5
10	65.0
11	79.0
12	107.0
13	151.0
14	215.0
15	328.0
16	467.5
17	667.5
18	960.5
19	1343.0
20	1939.5
21	2848.5
22	4109.5
23	5878.5
24	7952.5
25	10500.0
26	13756.0
27	17534.0
28	21814.5
29	26329.0
30	31246.0
31	36843.0
32	42549.5
33	48500.0
34	55693.5
35	63825.5
36	73401.5
37	82476.5
38	88248.0
39	89100.0
40	85732.5
41	80949.0
42	74592.0
43	68066.0
44	62708.5
45	57402.0
46	52187.5
47	46994.5
48	40977.0
49	34926.5
50	29736.0
51	25806.5
52	22576.0
53	19630.0
54	16911.5
55	14305.0
56	12293.5
57	10388.0
58	8524.0
59	6894.0
60	5436.0
61	4420.5
62	3534.5
63	2491.5
64	1654.5
65	1235.5
66	940.5
67	647.5
68	491.5
69	532.5
70	545.5
71	433.5
72	341.5
73	273.5
74	222.0
75	172.5
76	108.5
77	72.0
78	49.5
79	27.5
80	22.0
81	18.5
82	12.5
83	14.5
84	15.0
85	11.0
86	7.5
87	6.5
88	8.0
89	6.5
90	5.5
91	4.0
92	5.5
93	6.0
94	3.5
95	5.0
96	4.5
97	3.5
98	3.0
99	1.0
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0327320647635715
2	0.031223372399719514
3	0.027287653189670833
4	0.02604134210648875
5	0.03155134900055691
6	0.0017710736445219052
7	5.247625613398238E-4
8	0.0032141706882064204
9	4.591672411723458E-4
10-14	0.00640210324834585
15-19	0.024729435703139194
20-24	0.02075435930099003
25-29	0.002164645565526773
30-34	0.0019547405409908432
35-39	0.005654316598436601
40-44	0.013119064033495595
45-49	0.007543461819259968
50-54	0.005851102558939035
55-59	0.004263695810886068
60-64	0.010127917433858599
65-69	0.01387996974743834
70-74	0.01834045151882684
75-79	0.008146938764800764
80-84	0.011951467334514486
85-89	0.010101679305791608
90-94	0.01226632487131838
95-99	0.0104165368425955
100-101	0.007674652459594923
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1524499.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	26.461725535096942
#Duplication Level	Relative count
1	100.0
2	7.215188345624178
3	2.817640882044102
4	1.8870771124763135
5	1.438089145836602
6	1.0922959941100503
7	0.9028037608776991
8	0.6958106526015956
9	0.5932193161382207
10++	4.053564747202877
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GTCTC	353320	3.1828544	5.5364246	90-94
GCTGC	221880	3.1039355	7.4373794	90-94
TCTCT	520660	3.0203469	4.393299	80-84
CTGTC	327065	2.9463384	5.066318	90-94
AGAAG	410140	2.7765124	5.0373917	5
GACGC	193475	2.7744386	7.0953307	85-89
CGCTG	192335	2.6906228	6.9570227	90-94
GCCGA	183940	2.637706	7.360981	90-94
ACACA	420170	2.6253965	7.4995527	6
CTGCC	194575	2.6150665	6.926297	90-94
CGACG	177915	2.5513077	7.4836373	95-97
CCGAC	176655	2.4337575	6.8266997	95-97
TGCCG	172310	2.4104884	6.9217434	90-94
TACAC	375220	2.2871785	9.509179	5
CACAT	366540	2.2342691	5.2558713	7
CTGAC	233985	2.160689	5.1983075	95-97
TGACG	218675	2.101853	5.169933	95-97
CTTTG	346660	2.0931733	8.462323	9
GACGA	207000	2.0395257	5.4795666	95-97
GAGAG	177145	1.8167142	6.346105	7
GCTTT	290580	1.7545558	5.169591	1
TGGCT	169600	1.5902789	5.020036	6
TATAC	386620	1.5796154	5.808924	5
CACCA	169985	1.5458659	5.1423144	7
TTGAG	238995	1.539731	5.467445	9
GACTC	158780	1.4662231	5.100666	7
GAGTC	151715	1.4582489	7.318012	9
GTGTA	226025	1.4561715	10.716022	1
AAGAC	218095	1.4184511	6.0478864	5
CCATG	148320	1.3696324	6.188746	9
GTCTT	213380	1.2884132	6.4817586	1
TGAGT	198845	1.2810638	5.27388	8
TATGA	301045	1.2802573	6.1330094	4
GTGTT	203505	1.2790143	5.987587	1
GACTT	205360	1.2710805	6.6125746	7
GATTG	191530	1.2339367	6.1392813	7
TCATA	300465	1.2276112	6.2250175	2
GTTCT	193630	1.1691604	5.2867484	1
ACACC	124215	1.1296275	5.6758394	6
ACCAT	185100	1.1282895	5.211565	8
TGGAC	115475	1.1099186	7.4599514	5
ACATG	174805	1.1090901	5.6707435	8
GTCCA	115425	1.0658698	7.6238413	1
GGACT	108805	1.0458082	7.0989394	6
ACACT	170190	1.0374045	5.001552	6
AGACT	160470	1.0181383	5.572136	6
GTATG	153490	0.98886305	5.6625795	3
GAGTA	149630	0.9881673	5.29535	1
CATAC	149615	0.91198826	5.1181283	3
GTATA	180070	0.7657857	7.2386775	1
>>END_MODULE