##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-119_AGGCAGAA-AAGGAGTA_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1524499	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.48738569195519	34.0	31.0	34.0	31.0	34.0
2	32.67443337122556	34.0	31.0	34.0	31.0	34.0
3	32.78540359816569	34.0	31.0	34.0	31.0	34.0
4	36.279802741753194	37.0	37.0	37.0	35.0	37.0
5	36.0151170974858	37.0	35.0	37.0	35.0	37.0
6	36.1081765222542	37.0	37.0	37.0	35.0	37.0
7	36.104176519630386	37.0	36.0	37.0	35.0	37.0
8	36.124192931579486	37.0	36.0	37.0	35.0	37.0
9	37.99516890466967	39.0	38.0	39.0	35.0	39.0
10-14	38.188303567270296	39.4	38.2	39.4	35.2	39.4
15-19	39.340576937079	41.0	39.0	41.0	36.0	41.0
20-24	39.274002278781424	40.2	39.0	41.0	36.0	41.0
25-29	39.09512698926007	40.0	39.0	41.0	36.0	41.0
30-34	38.90822007754679	40.0	38.2	41.0	35.4	41.0
35-39	38.6935609665864	40.0	38.0	41.0	35.0	41.0
40-44	38.475404969107885	40.0	38.0	41.0	34.2	41.0
45-49	38.305739787300624	40.0	38.0	41.0	33.8	41.0
50-54	38.254768025429996	40.0	37.8	41.0	34.0	41.0
55-59	37.886651680322515	40.0	36.8	41.0	33.0	41.0
60-64	37.34450832699792	39.0	35.8	40.8	32.6	41.0
65-69	36.61935324326221	38.0	35.0	40.0	31.2	41.0
70-74	36.02579457251202	36.8	35.0	39.2	31.0	41.0
75-79	34.82440185267423	35.2	34.0	37.4	30.4	39.2
80-84	34.59189530462139	35.0	34.0	36.6	31.0	38.4
85-89	33.953879668008966	35.0	34.0	35.8	30.6	36.8
90-94	33.54011750745655	35.0	34.0	35.0	30.0	36.0
95-99	33.26889712620343	35.0	34.0	35.0	29.4	35.6
100-101	32.92980120026317	35.0	33.5	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	2.0
13	6.0
14	16.0
15	53.0
16	116.0
17	227.0
18	411.0
19	716.0
20	1073.0
21	1532.0
22	2188.0
23	3077.0
24	4236.0
25	5371.0
26	7197.0
27	9222.0
28	11933.0
29	15416.0
30	19168.0
31	24537.0
32	31307.0
33	42687.0
34	61006.0
35	97442.0
36	182359.0
37	366359.0
38	516673.0
39	120030.0
40	139.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.94466444385992	23.65203256938837	11.186691496681862	24.21661149006985
2	15.244380065419936	23.451459221442413	40.0503573429482	21.253803370189452
3	15.981119043042993	27.126616678659676	33.14288825378042	23.74937602451691
4	11.332772274694834	18.401455166582593	44.040763555764876	26.225009002957695
5	10.403876945803178	39.27034389658504	39.14971410279705	11.17606505481473
6	25.417399421055702	43.89455158711157	19.482531638262802	11.205517353569928
7	23.918218378627994	33.872832976604116	26.63255272715823	15.576395917609654
8	21.001588062701256	38.72675547835715	24.19949111150614	16.07216534743545
9	22.606836737839775	15.106274257969341	25.401722139535675	36.885166864655204
10-14	20.261594136827902	26.11095186025048	32.904160645562904	20.723293357358713
15-19	19.445481956280716	32.04199515559594	28.850298258961114	19.66222462916222
20-24	19.446652107895137	30.2567105170288	30.569851798073007	19.726785577003053
25-29	19.450619527550376	30.76273466008322	30.082179414338817	19.70446639802759
30-34	19.30171043773871	30.49673397956197	30.165953224410448	20.03560235828888
35-39	19.492014376327568	30.77181937402286	30.034365848767163	19.7018004008824
40-44	19.192335378009588	30.462202191407112	30.50189512769207	19.843567302891234
45-49	19.408346764544856	30.268549412091623	30.44551456602256	19.877589257340965
50-54	19.066205420106492	29.920423811295816	30.872095961336143	20.14127480726155
55-59	19.25064402782641	29.874688223333678	30.83116207451771	20.0435056743222
60-64	18.841978312426853	29.459902981896917	31.096564987900305	20.601553717775925
65-69	19.196167247569683	29.708136264675478	30.37129231365298	20.724404174101856
70-74	19.104534241487464	29.49897280326247	30.2296278988182	21.166865056431863
75-79	19.416887016037673	29.973426882601395	29.62105593211556	20.988630169245372
80-84	19.334710225001487	29.923385857338086	29.29774982771362	21.444154089946807
85-89	19.410158108253132	30.039334862716316	29.091438402801483	21.459068626229065
90-94	19.306904454159838	29.481317109290835	29.49965810700962	21.71212032953971
95-99	19.46033824729645	29.53448522642727	29.06024473212982	21.94493179414646
100-101	19.365194313144393	28.66051644541351	29.920734563115204	22.053554678326897
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.16127593392977
2	36.49818343560939
3	39.7304950675599
4	37.55778127765252
5	21.57994200061791
6	36.62291677462563
7	39.49461429623765
8	37.0737534101367
9	59.49200360249498
10-14	40.984887494186616
15-19	39.10770658544293
20-24	39.1734376848982
25-29	39.155085925577964
30-34	39.33731279602758
35-39	39.19381477720997
40-44	39.03590268090082
45-49	39.28593602188582
50-54	39.20748022736804
55-59	39.29414970214861
60-64	39.443532030202775
65-69	39.92057142167154
70-74	40.27139929791933
75-79	40.405517185283045
80-84	40.778864314948294
85-89	40.8692267344822
90-94	41.01902478369955
95-99	41.40527004144291
100-101	41.4187489914713
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	2.5
7	5.5
8	7.5
9	13.0
10	23.5
11	41.0
12	66.0
13	114.0
14	177.5
15	264.5
16	404.5
17	628.5
18	933.0
19	1322.5
20	1899.0
21	2784.0
22	4028.5
23	5811.0
24	8089.5
25	10596.5
26	13571.5
27	17311.0
28	21561.5
29	26087.0
30	30988.0
31	36330.5
32	41707.5
33	47653.0
34	54490.5
35	62451.5
36	72084.5
37	81135.5
38	87090.0
39	88334.5
40	85155.5
41	79886.0
42	73707.0
43	67825.0
44	63129.5
45	58226.5
46	53524.0
47	48664.5
48	42733.5
49	36435.0
50	30987.0
51	27182.0
52	23527.5
53	20333.0
54	17554.0
55	14764.5
56	12727.5
57	10862.5
58	8884.0
59	7057.0
60	5561.5
61	4547.0
62	3529.0
63	2393.5
64	1624.5
65	1229.0
66	889.0
67	603.0
68	439.0
69	459.0
70	472.0
71	353.5
72	326.5
73	309.5
74	217.5
75	165.0
76	98.5
77	52.5
78	27.5
79	12.0
80	6.0
81	4.0
82	2.0
83	0.5
84	1.0
85	1.0
86	0.5
87	0.5
88	0.5
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.012659896792323249
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0012069538910815948
20-24	7.477866499092489E-4
25-29	0.017907522405721486
30-34	0.006926865809685674
35-39	0.04333226850263595
40-44	0.05262056583835083
45-49	0.05307973307952318
50-54	0.052607446774317335
55-59	0.04275502968516214
60-64	0.05408990101010234
65-69	0.043594649783305864
70-74	0.0038570048258477047
75-79	0.001705478324354427
80-84	1.5742876840194712E-4
85-89	0.0032666469443404034
90-94	0.0029780275356035
95-99	0.00265005093476611
100-101	6.559532016747798E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1524499.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	27.203437357862345
#Duplication Level	Relative count
1	100.0
2	7.521383075523203
3	2.8632089778586596
4	1.9077949651198058
5	1.4121929026387625
6	1.1555959963603275
7	0.8996057021534729
8	0.6988171064604186
9	0.6138914164391871
10++	4.249924173491053
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	227165	3.201625	7.3644147	95-97
GTCTC	354415	3.1993752	5.538829	90-94
CTCCG	239240	3.155829	7.4448743	95-97
GAGCC	223325	3.147505	7.321799	90-94
TCTCT	522320	3.0496924	4.4961004	80-84
CGAGC	212105	2.9893723	7.278048	85-89
CTGTC	328140	2.9621854	5.0949874	90-94
TCTCC	332025	2.8328054	5.4896116	95-97
AGCCC	208755	2.7807302	6.900696	90-94
GCCCA	207685	2.7664769	7.0427914	90-94
ACACA	420955	2.5309398	7.701463	6
CCCAC	199575	2.5125859	6.511086	90-94
ATCTC	423280	2.4956825	5.766234	95-97
CCACG	180745	2.4076214	6.624254	90-94
TCCGA	254665	2.3214784	5.1625338	95-97
TACAC	376965	2.244424	9.710781	5
CACAT	368520	2.1941433	5.467367	7
GAGAC	224140	2.1830523	5.49381	95-97
CTTTG	346345	2.139614	8.976512	9
CGAGA	217145	2.1149232	5.4969587	95-97
GGCAG	140370	2.093199	7.145262	90-94
CAGGC	140240	1.976519	6.720419	90-94
ACGAG	202505	1.9723344	5.3049116	95-97
AGAGA	277355	1.8667943	5.133145	8
GAGAG	176155	1.8152939	6.160406	7
GCTTT	292945	1.8097252	5.370966	1
TGGCT	169985	1.6235695	5.2003717	6
TATAC	390110	1.589514	5.938279	5
TTGAG	235570	1.5548767	5.6962667	9
CACCA	169945	1.4785597	5.057594	7
GAGTC	152030	1.4663303	7.234144	9
GACTC	159675	1.4555672	5.1179757	7
GTCTT	227810	1.4073409	6.3445096	1
AAGAC	216195	1.3753048	5.6935	5
CATGA	216225	1.362125	5.173909	9
CCATG	145855	1.3295867	5.798605	9
GTGTT	201595	1.3176906	6.1201434	1
TGAGT	199575	1.317292	5.2642455	8
TATGA	301340	1.299096	6.506657	4
GACTT	202495	1.263232	6.5663333	7
GATTG	189980	1.2539604	6.5667067	7
GTATG	188615	1.2449507	5.920276	3
TCATA	303740	1.2375972	6.31239	2
GTTCT	194215	1.1998012	5.4069123	1
GTGTA	176090	1.1622797	11.28506	1
TGGAC	115895	1.117808	7.776588	5
ACATG	176460	1.1116226	5.7236753	8
GTCCA	118630	1.0814089	7.8193173	1
ACACC	123145	1.0713892	5.538466	6
GGACT	108505	1.0465314	7.2622013	6
ACACT	173110	1.0306852	5.2046785	6
AGACT	158865	1.0007815	5.427412	6
GAGTA	149165	0.99422604	5.3327074	1
TGTAT	231015	0.98623955	5.082055	2
GCCGT	70005	0.977048	5.046105	95-97
CATAC	151560	0.9023781	5.262413	3
TGCCG	63820	0.89072496	5.0618954	95-97
GTATA	178520	0.7696111	7.096919	1
>>END_MODULE