##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-118_AGGCAGAA-ACTGCATA_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	2733160	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.503867318415313	31.0	31.0	34.0	30.0	34.0
2	31.704433695795345	33.0	31.0	34.0	30.0	34.0
3	31.842977359539873	34.0	31.0	34.0	30.0	34.0
4	35.37810958743725	37.0	35.0	37.0	33.0	37.0
5	35.24811646592223	37.0	35.0	37.0	32.0	37.0
6	35.39369850283189	37.0	35.0	37.0	33.0	37.0
7	35.40298555518155	37.0	35.0	37.0	33.0	37.0
8	35.4566812773493	37.0	35.0	37.0	33.0	37.0
9	37.23062425909936	39.0	37.0	39.0	34.0	39.0
10-14	37.33382846229273	39.2	37.2	39.4	33.2	39.4
15-19	38.359323859561826	40.0	38.0	41.0	33.2	41.0
20-24	38.24979379180143	40.0	38.0	41.0	33.4	41.0
25-29	38.06209449867553	40.0	38.0	41.0	33.0	41.0
30-34	37.791048529906774	40.0	38.0	41.0	32.0	41.0
35-39	37.47560303824145	40.0	37.0	41.0	31.2	41.0
40-44	37.4261229492602	40.0	37.0	41.0	31.0	41.0
45-49	37.258437852156476	40.0	36.8	41.0	31.0	41.0
50-54	36.5894007668779	38.6	35.4	40.2	30.2	40.6
55-59	37.21876077507354	39.8	36.0	41.0	31.0	41.0
60-64	36.761485972281164	39.0	35.2	41.0	30.6	41.0
65-69	36.08984428280818	37.8	35.0	40.0	29.6	41.0
70-74	35.2724929385766	36.4	34.2	39.2	29.0	41.0
75-79	34.349054940069365	35.4	34.0	37.6	28.4	39.4
80-84	33.482935137350175	35.0	33.4	36.4	27.4	38.2
85-89	32.76957836350598	35.0	33.0	35.4	26.0	36.6
90-94	32.25660773610034	35.0	32.4	35.0	25.4	36.0
95-99	31.82459899896091	35.0	32.0	35.0	24.6	35.2
100-101	31.01256165025099	34.0	30.5	35.0	22.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	8.0
6	64.0
7	288.0
8	568.0
9	802.0
10	985.0
11	1373.0
12	2285.0
13	3314.0
14	4120.0
15	5077.0
16	5739.0
17	6616.0
18	7326.0
19	8401.0
20	9459.0
21	10771.0
22	12058.0
23	13957.0
24	16185.0
25	18280.0
26	21141.0
27	25306.0
28	29850.0
29	35556.0
30	43944.0
31	54534.0
32	70486.0
33	94903.0
34	134082.0
35	206220.0
36	357156.0
37	642731.0
38	745725.0
39	143757.0
40	93.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.619756560338715	23.39896844127172	10.730018856764719	24.251256141624843
2	15.103326005974615	23.49549415019109	40.17943610814039	21.2217437356939
3	15.69111772977175	27.863422660852848	32.72752825231981	23.717931357055598
4	11.090851864322417	18.31958453498075	44.304557232575505	26.285006368121334
5	10.647683185711148	39.38595271210014	38.81714369372667	11.149220408462044
6	25.54312649577716	43.91160841985561	19.399138929959303	11.146126154407925
7	23.907470156000688	33.94772072595183	26.74396268891809	15.400846429129396
8	21.113813802606437	38.78897711664285	24.118777437014238	15.978431643736476
9	22.8644175824015	15.19964934130755	25.23875451769429	36.69717855859666
10-14	20.399625753350676	26.03967058391332	32.77643226732071	20.784271395415296
15-19	19.653662138041092	31.8445595999033	28.7009761100039	19.80080215205171
20-24	19.619720217963955	30.190295786582045	30.38408202360932	19.805901971844676
25-29	19.579154590324695	30.641463136383006	29.95456512253162	19.82481715076068
30-34	19.50779415201871	30.398092949677224	30.001781127396566	20.0923317709075
35-39	19.661843261215818	30.649408908109166	29.896256594876018	19.792491235798995
40-44	19.37872970753677	30.474958174582152	30.279450875808884	19.866861242072197
45-49	19.57951519721605	30.345744276015825	30.20094685482379	19.87379367194434
50-54	19.388798577630613	29.999211128272446	30.56322514179567	20.04876515230127
55-59	19.551283435103706	29.98720622636659	30.61753539509475	19.843974943434954
60-64	19.292958958022595	29.638452007186306	30.858687734858805	20.209901299932298
65-69	19.536185066366702	29.831390921522516	30.42151720725959	20.21090680485119
70-74	19.471803836483073	29.54857521556843	30.534084201504836	20.445536746443665
75-79	19.67447197911298	29.805471053885864	30.229327965167023	20.290729001834134
80-84	19.668022195376896	29.67342533130342	30.131634383850397	20.526918089469287
85-89	19.850172739125576	29.588761740610874	30.031879604254403	20.529185916009148
90-94	19.825233504512894	29.182128982720357	30.459015995135974	20.533621517630774
95-99	20.16225580013221	29.127929046367175	29.97941450065436	20.730400652846253
100-101	20.28748388210998	28.479781104051682	30.65278735215815	20.579947661680183
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.87101270196356
2	36.325069741668514
3	39.409049086827345
4	37.37585823244375
5	21.796903594173195
6	36.68925265018508
7	39.30831658513008
8	37.092245446342915
9	59.56159614099816
10-14	41.18389714876597
15-19	39.4544642900928
20-24	39.42562218980863
25-29	39.40397174108538
30-34	39.60012592292621
35-39	39.45433449701481
40-44	39.245590949608975
45-49	39.45330886916039
50-54	39.43756372993189
55-59	39.395258378538664
60-64	39.50286025795489
65-69	39.74709187121789
70-74	39.91734058292674
75-79	39.96520098094712
80-84	40.19494028484618
85-89	40.37935865513472
90-94	40.358855022143665
95-99	40.892656452978464
100-101	40.86743154379017
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	5.0
2	8.5
3	16.0
4	20.0
5	26.5
6	37.5
7	48.5
8	67.0
9	79.5
10	97.5
11	132.5
12	198.5
13	283.0
14	399.5
15	548.0
16	765.5
17	1104.5
18	1575.5
19	2307.0
20	3383.0
21	5019.0
22	7366.0
23	10413.0
24	14092.5
25	18704.0
26	24729.5
27	31853.0
28	39805.0
29	47997.0
30	57335.5
31	67547.5
32	77499.5
33	88519.5
34	101160.5
35	115276.5
36	131387.0
37	146081.5
38	154639.5
39	156386.0
40	151700.0
41	142918.0
42	131678.5
43	120230.0
44	110897.5
45	101361.5
46	91401.5
47	81811.5
48	71895.0
49	61485.0
50	52247.5
51	46334.0
52	41443.0
53	36486.0
54	31695.5
55	27392.0
56	24255.0
57	20752.0
58	17003.0
59	13940.0
60	11376.5
61	9280.0
62	7291.5
63	5019.5
64	3429.5
65	2697.0
66	1943.0
67	1342.0
68	989.5
69	1036.0
70	1086.0
71	854.0
72	720.0
73	607.0
74	476.0
75	350.0
76	228.5
77	145.5
78	97.5
79	56.0
80	40.5
81	33.5
82	25.0
83	22.0
84	19.5
85	17.5
86	13.0
87	13.5
88	15.0
89	12.5
90	12.0
91	8.5
92	6.0
93	6.0
94	8.5
95	11.0
96	9.0
97	7.0
98	6.5
99	3.0
100	1.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03600228307160942
2	0.03417289876919024
3	0.02992872718757775
4	0.029270148838706843
5	0.03512417860644822
6	0.0017927966163707944
7	0.0011342182674998903
8	0.0030001902559674515
9	6.58578348870904E-4
10-14	0.007427300267821862
15-19	0.024169825403562176
20-24	0.020540326947562526
25-29	0.00224648392337075
30-34	0.00218062608848366
35-39	0.006110143570080054
40-44	0.013610619209998684
45-49	0.00872250435393464
50-54	0.005224721567709171
55-59	0.004090503300209282
60-64	0.010778732309853796
65-69	0.014232609872821204
70-74	0.01833043071024016
75-79	0.007661461458531517
80-84	0.011071433798240864
85-89	0.009249367033031364
90-94	0.01021528194470869
95-99	0.009710371877240996
100-101	0.006293082000321971
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2733160.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	31.83920685622524
#Duplication Level	Relative count
1	100.0
2	8.662612035851472
3	2.9878361075544175
4	2.021766965428937
5	1.5512163892445583
6	1.2215108834827144
7	1.0685019206145967
8	0.9513444302176697
9	0.8245838668373879
10++	8.751600512163892
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AGAAG	705205	2.5778475	5.1637616	5
GCTGC	317480	2.472849	5.609103	90-94
ACACA	629015	2.2006655	7.246264	6
CTGCC	271025	2.0652196	5.290079	90-94
GACGC	258355	2.0437784	5.5496707	95-97
GCCGA	257940	2.0404956	5.511846	90-94
CTTTG	596885	2.0375156	7.757226	9
CGACG	255140	2.0183456	5.5944366	95-97
CGCTG	258655	2.0146616	5.3597364	95-97
CCGAC	245705	1.901545	5.222827	95-97
TACAC	550165	1.8951793	9.194136	5
TGCCG	233880	1.8216896	5.211692	90-94
GAGAG	323695	1.7792614	5.883091	7
GCTTT	504630	1.7225955	5.082101	1
ATACA	677760	1.5870686	5.0589337	6
TTGAG	413215	1.4643553	5.06631	9
GACTC	274145	1.4200369	5.08679	7
GAGTC	262350	1.389072	6.971198	9
CCATG	263335	1.3640423	5.659452	9
AAGAC	375145	1.3415802	5.5947433	5
TATAC	574730	1.3250978	5.9311466	5
GTCTT	373815	1.2760481	6.449914	1
GTGTA	356940	1.2649275	10.149693	1
GACTT	356595	1.2362926	6.014014	7
GTGTT	350085	1.2215413	5.795682	1
TATGA	518200	1.2212535	5.713966	4
TCATA	524265	1.2087456	5.9064436	2
GATTG	338805	1.2006605	5.5097685	7
GTTCT	345095	1.17801	5.3934197	1
ACACC	216730	1.1154488	5.121527	6
TGGAC	206655	1.094182	7.0325055	5
ACATG	310710	1.0940495	5.254283	8
GTCCA	207085	1.0726745	7.6891246	1
ACACT	297650	1.025329	5.218671	6
GGACT	191305	1.012908	6.5320916	6
AGACT	282285	0.9939614	5.2831573	6
GAGTA	265160	0.9543642	5.1855965	1
GTATG	266630	0.944886	5.060936	3
GTATA	315515	0.74358124	7.403744	1
>>END_MODULE