##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-118_AGGCAGAA-ACTGCATA_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	2733160	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47929576021894	34.0	31.0	34.0	31.0	34.0
2	32.66867728197398	34.0	31.0	34.0	31.0	34.0
3	32.77961443896442	34.0	31.0	34.0	31.0	34.0
4	36.275456248445025	37.0	37.0	37.0	35.0	37.0
5	36.01194734300224	37.0	35.0	37.0	35.0	37.0
6	36.10391817529892	37.0	36.0	37.0	35.0	37.0
7	36.10249491431164	37.0	36.0	37.0	35.0	37.0
8	36.12598750164645	37.0	36.0	37.0	35.0	37.0
9	37.98889710079176	39.0	38.0	39.0	35.0	39.0
10-14	38.18015652212092	39.4	38.2	39.4	35.2	39.4
15-19	39.32557764638733	41.0	39.0	41.0	36.0	41.0
20-24	39.25817837228702	40.2	39.0	41.0	36.0	41.0
25-29	39.07111570489836	40.0	39.0	41.0	36.0	41.0
30-34	38.87975149643636	40.0	38.2	41.0	35.2	41.0
35-39	38.664290052539926	40.0	38.0	41.0	35.0	41.0
40-44	38.43361998565762	40.0	38.0	41.0	34.0	41.0
45-49	38.25683099416061	40.0	38.0	41.0	33.6	41.0
50-54	38.19017437691171	40.0	37.6	41.0	33.6	41.0
55-59	37.803070877665405	39.8	36.6	41.0	33.0	41.0
60-64	37.247918233839215	39.0	35.6	40.8	32.4	41.0
65-69	36.513034655856224	37.8	35.0	40.0	31.0	41.0
70-74	35.93699980974403	36.6	35.0	39.2	31.0	41.0
75-79	34.76282237410177	35.2	34.0	37.4	30.4	39.2
80-84	34.55832977213189	35.0	34.0	36.6	31.0	38.4
85-89	33.937988043144195	35.0	34.0	35.6	30.6	36.8
90-94	33.53137284315591	35.0	34.0	35.0	30.0	36.0
95-99	33.26220974988657	35.0	34.0	35.0	29.4	35.6
100-101	32.93148626498265	35.0	33.5	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	4.0
11	0.0
12	1.0
13	7.0
14	41.0
15	93.0
16	216.0
17	400.0
18	760.0
19	1256.0
20	1917.0
21	2886.0
22	4034.0
23	5603.0
24	7878.0
25	10010.0
26	12849.0
27	16818.0
28	21607.0
29	27361.0
30	34766.0
31	44007.0
32	57325.0
33	77726.0
34	113022.0
35	180323.0
36	337208.0
37	667149.0
38	902698.0
39	204986.0
40	209.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.02906525779684	23.562360052100864	11.087751906218443	24.320822783883855
2	15.317977348415848	23.375972312534923	39.99317181092283	21.312878528126394
3	15.875214037963383	27.44453306795065	32.956504558825685	23.723748335260282
4	11.25642113890149	18.28594008400533	44.04418328967203	26.413455487421157
5	10.41739232243996	39.471893339577626	38.98710649943655	11.123607838545858
6	25.613977959577927	44.013632571821624	19.24830599013596	11.124083478464488
7	23.854146848336725	33.94795767536478	26.711096313424754	15.486799162873744
8	21.07179967510135	38.917699659002764	23.97869133164542	16.031809334250465
9	22.731490289628123	15.066004185631284	25.131679082088134	37.07082644265246
10-14	20.357051910608963	25.993019069501965	32.7801738646841	20.869755155204963
15-19	19.598661810884412	31.893358463040716	28.69906785138075	19.808911874694125
20-24	19.550034356063463	30.196338133913276	30.397344887312844	19.85628262271042
25-29	19.582696809190743	30.6525557805931	29.91467935613971	19.85006805407644
30-34	19.464005837474687	30.401242958831563	30.013158614025677	20.121592589668076
35-39	19.604656786075587	30.648495140439085	29.914504260950913	19.83234381253441
40-44	19.369429720195093	30.464590272961008	30.25260354550456	19.913376461339347
45-49	19.56388205488787	30.331379822631927	30.172823026982986	19.931915095497214
50-54	19.35370957930239	29.997811669286346	30.552859900783474	20.095618850627798
55-59	19.499488325857403	29.911052373777835	30.63533903691938	19.954120263445382
60-64	19.165765155779326	29.61890103724026	30.869831589761493	20.34550221721893
65-69	19.400156621313545	29.808665622866215	30.34782830866185	20.44334944715839
70-74	19.318565835897367	29.631440918423753	30.32129639644162	20.72869684923726
75-79	19.52665978027755	29.978894456476272	29.862229287487036	20.63221647575914
80-84	19.46560374869281	29.942132086598516	29.57633510337539	21.015929061333285
85-89	19.52215121304079	30.02900250992125	29.371918167742123	21.076928109295842
90-94	19.42110844266432	29.60185536367928	29.721200038549746	21.255836155106657
95-99	19.53428227371024	29.68827018837527	29.291526215529224	21.485921322385266
100-101	19.44378281687966	28.99670308240391	30.03802559602277	21.521488504693654
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.34988804168069
2	36.63085587654224
3	39.59896237322367
4	37.66987662632265
5	21.541000160985817
6	36.738061438042415
7	39.34094601121046
8	37.103609009351814
9	59.80231673228058
10-14	41.22680706581393
15-19	39.40757368557854
20-24	39.40631697877388
25-29	39.43276486326718
30-34	39.58559842714276
35-39	39.43700059861
40-44	39.28280618153443
45-49	39.49579715038509
50-54	39.44932842993018
55-59	39.45360858930279
60-64	39.51126737299825
65-69	39.84350606847193
70-74	40.047262685134626
75-79	40.15887625603669
80-84	40.481532810026096
85-89	40.59907932233663
90-94	40.676944597770984
95-99	41.020203596095506
100-101	40.96527132157332
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.0
6	5.0
7	9.0
8	12.5
9	22.5
10	46.5
11	78.5
12	131.5
13	207.5
14	312.0
15	476.0
16	708.0
17	1064.5
18	1579.5
19	2272.5
20	3380.0
21	5147.0
22	7458.5
23	10566.5
24	14448.5
25	19046.0
26	24836.0
27	31400.5
28	39416.5
29	47909.5
30	56832.0
31	66555.0
32	76495.0
33	87578.5
34	100191.5
35	114248.0
36	130099.0
37	144693.0
38	153317.0
39	154969.0
40	150158.0
41	141175.5
42	130195.0
43	120022.5
44	111298.5
45	101966.5
46	92844.5
47	83904.5
48	74076.5
49	63124.5
50	53764.0
51	47903.0
52	42566.0
53	37546.0
54	32649.5
55	28030.5
56	24967.0
57	21401.0
58	17373.0
59	14322.5
60	11538.5
61	9316.5
62	7341.0
63	4964.5
64	3333.0
65	2513.5
66	1806.5
67	1236.5
68	898.0
69	953.0
70	1059.0
71	858.5
72	707.5
73	615.0
74	477.0
75	358.5
76	206.0
77	100.0
78	37.5
79	12.5
80	8.0
81	5.5
82	2.5
83	2.5
84	2.0
85	2.0
86	2.0
87	0.5
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.013574031523950298
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0012293462512256875
20-24	6.58578348870904E-4
25-29	0.017569406840433784
30-34	0.007222409225950913
35-39	0.04259538409752814
40-44	0.050681262714220895
45-49	0.05101786942586604
50-54	0.05112763248401118
55-59	0.04199534604633465
60-64	0.053037509695736805
65-69	0.044644294516237616
70-74	0.0034685126373867614
75-79	0.0014342372930966354
80-84	1.6830335582256436E-4
85-89	0.003153858537370662
90-94	0.0024806451140804054
95-99	0.002283071609419134
100-101	3.6587686048383556E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2733160.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	32.88310944993193
#Duplication Level	Relative count
1	100.0
2	9.249365264973608
3	3.0518964525903005
4	1.9930356808300331
5	1.5440373669963627
6	1.3263216378424822
7	1.1060217457312858
8	0.9606625793822559
9	0.8947664239706956
10++	9.082040713487393
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	330845	2.5903914	6.00332	95-97
CTCCG	340490	2.542904	5.8542776	95-97
GAGCC	323785	2.535114	5.694549	95-97
CGAGC	298775	2.3392954	5.6477275	95-97
GCCCA	302810	2.2752752	5.4424734	90-94
AGCCC	300920	2.261074	5.2464595	90-94
ACACA	637140	2.1567087	7.2950296	6
CTTTG	597935	2.0709672	8.428205	9
CCCAC	284560	2.0519202	5.1313834	90-94
CCACG	249830	1.8771905	5.129873	90-94
TACAC	556565	1.8725542	9.237698	5
AGAGA	504320	1.8536044	5.019915	8
GAGAG	320395	1.7909713	5.905529	7
GCTTT	507995	1.7594568	5.1528354	1
TTGAG	415165	1.5074925	5.0138702	9
GAGTC	263285	1.4038275	6.889434	9
CCATG	266925	1.36584	5.6564517	9
GTCTT	390445	1.3523186	6.4259286	1
TATAC	580150	1.3292466	6.169782	5
AAGAC	376450	1.3278257	5.7135873	5
GTGTT	351820	1.2697457	5.9136577	1
TGAGT	348005	1.2636302	5.0279546	8
TATGA	520115	1.2417717	5.853748	4
GACTT	354535	1.2354251	6.158197	7
GATTG	338285	1.228336	5.7145443	7
TCATA	524845	1.2025311	5.873057	2
GTTCT	345605	1.1970139	5.520655	1
GTATG	306090	1.1114339	5.2832255	3
GTGTA	304730	1.1064955	10.309394	1
ACATG	312135	1.0943041	5.135053	8
TGGAC	203860	1.0869752	7.160875	5
ACACC	219720	1.0855285	5.1691813	6
GTCCA	209815	1.0736113	7.5754614	1
GGACT	190390	1.0151536	6.757591	6
ACACT	297800	1.0019432	5.146553	6
AGACT	279340	0.97932917	5.2540383	6
GAGTA	266120	0.97218776	5.19762	1
GTATA	317035	0.7569194	7.1201525	1
ATACT	326860	0.74890554	5.052245	6
>>END_MODULE