##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-117_AGGCAGAA-GTAAGGAG_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1555051	
Filtered Sequences	0	
Sequence length	101	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.536244791971452	31.0	31.0	34.0	30.0	34.0
2	31.742462465861248	33.0	31.0	34.0	30.0	34.0
3	31.88074217501548	34.0	31.0	34.0	30.0	34.0
4	35.4173322932817	37.0	35.0	37.0	33.0	37.0
5	35.28263060182592	37.0	35.0	37.0	32.0	37.0
6	35.426331998114534	37.0	35.0	37.0	33.0	37.0
7	35.44019135063738	37.0	35.0	37.0	33.0	37.0
8	35.49146683935125	37.0	35.0	37.0	33.0	37.0
9	37.27307528820598	39.0	37.0	39.0	34.0	39.0
10-14	37.389839818758354	39.2	37.2	39.4	33.2	39.4
15-19	38.42307306962923	40.0	38.0	41.0	33.6	41.0
20-24	38.319564438722594	40.0	38.0	41.0	33.6	41.0
25-29	38.136243891679435	40.0	38.0	41.0	33.0	41.0
30-34	37.87128679380934	40.0	38.0	41.0	32.4	41.0
35-39	37.56027307142981	40.0	37.2	41.0	31.6	41.0
40-44	37.52312200693096	40.0	37.0	41.0	31.8	41.0
45-49	37.36527316467434	40.0	37.0	41.0	31.0	41.0
50-54	36.697679111488945	38.8	36.0	40.2	30.6	40.6
55-59	37.34121234609025	40.0	36.4	41.0	31.2	41.0
60-64	36.8899682389838	39.0	35.4	41.0	31.0	41.0
65-69	36.22491429541539	38.0	35.0	40.2	30.0	41.0
70-74	35.3903610878357	36.6	34.4	39.2	29.0	41.0
75-79	34.43568448880454	35.4	34.0	37.8	28.6	39.6
80-84	33.52516322615786	35.0	33.4	36.4	27.4	38.2
85-89	32.76642026531606	35.0	33.0	35.4	26.0	36.8
90-94	32.20631156148577	35.0	32.4	35.0	25.2	36.0
95-99	31.721468684949883	35.0	32.0	35.0	24.0	35.2
100-101	30.856596343142442	34.0	30.0	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	2.0
6	39.0
7	176.0
8	319.0
9	436.0
10	552.0
11	714.0
12	1122.0
13	1647.0
14	2147.0
15	2563.0
16	3075.0
17	3452.0
18	4045.0
19	4484.0
20	5206.0
21	5872.0
22	6777.0
23	7781.0
24	8901.0
25	10387.0
26	12051.0
27	14032.0
28	16890.0
29	20121.0
30	24870.0
31	30860.0
32	39820.0
33	53462.0
34	75256.0
35	115202.0
36	199147.0
37	366556.0
38	431095.0
39	85920.0
40	72.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.204268371133175	23.752528137592442	10.983503419740316	24.05970007153407
2	14.952497904555814	23.602002620672177	40.36857946574053	21.07692000903148
3	15.703759451697044	27.81724092460191	33.00000321623038	23.47899640747066
4	11.09182833324757	18.32224823753409	44.433888231359035	26.152035197859313
5	10.535507863082591	39.329950988811504	39.09650617576999	11.038034972335922
6	25.505250411409236	43.77220629174783	19.51960777378569	11.202935523057246
7	23.729360712622924	34.06308760395204	26.85994737126409	15.347604312160943
8	20.942339017995465	38.86487678801906	24.228313385890573	15.964470808094905
9	22.787300182759868	15.35858103232959	25.455131230844618	36.398987554065926
10-14	20.251007276831505	26.21579680179557	32.88727824635757	20.645917675015355
15-19	19.46634206811167	32.058622766616054	28.87773188560731	19.597303279664967
20-24	19.47745432681237	30.41426891382858	30.526675035163898	19.581601724195156
25-29	19.45589554742017	30.798545302638374	30.100688249444897	19.64487090049656
30-34	19.33655455332582	30.623818987466155	30.194731700189887	19.84489475901814
35-39	19.47397936150044	30.76227139253922	30.143206440302567	19.620542805657774
40-44	19.20785156034442	30.57196356797381	30.518735877071563	19.701448994610214
45-49	19.370582786903483	30.417951788121172	30.469041515805984	19.742423909169357
50-54	19.12447108413187	30.12455777372883	30.826409098240408	19.92456204389889
55-59	19.25201156074271	30.06040052049323	30.88889640592401	19.798691512840048
60-64	18.980819704709763	29.729579896030362	31.10046646776562	20.18913393149426
65-69	19.295407120947335	29.745816802970076	30.652213677300384	20.30656239878221
70-74	19.154980778937976	29.433167767892105	30.68108319213189	20.730768261038023
75-79	19.257384704332072	29.54752507657867	30.38884533585605	20.80624488323321
80-84	19.18770229076464	29.313434325595406	30.273432349877954	21.225431033762003
85-89	19.35041734189236	29.23213284968759	30.149072082655216	21.268377725764836
90-94	19.282056347934176	28.70385448188059	30.543038108519642	21.471051061665587
95-99	19.649735153965192	28.512626572171623	30.1045711264121	21.73306714745109
100-101	19.839700108137794	27.783696113181012	30.617787397171824	21.75881638150937
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.26396844266725
2	36.029417913587295
3	39.182755859167706
4	37.24386353110688
5	21.573542835418515
6	36.70818593446648
7	39.07696502478387
8	36.90680982609037
9	59.18628773682579
10-14	40.89692495184686
15-19	39.063645347776635
20-24	39.05905605100752
25-29	39.100766447916726
30-34	39.18144931234396
35-39	39.09452216715821
40-44	38.90930055495463
45-49	39.113006696072844
50-54	39.04903312803076
55-59	39.05070307358275
60-64	39.16995363620402
65-69	39.60196951972954
70-74	39.885749039976005
75-79	40.06362958756529
80-84	40.41313332452664
85-89	40.618795067657196
90-94	40.753107409599764
95-99	41.382802301416284
100-101	41.59851648964717
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	5.0
1	3.5
2	2.0
3	5.0
4	8.5
5	10.0
6	18.5
7	27.5
8	38.0
9	47.5
10	57.0
11	84.5
12	119.0
13	174.0
14	254.0
15	348.0
16	488.0
17	678.0
18	939.5
19	1373.0
20	2029.5
21	3012.0
22	4407.5
23	6313.0
24	8645.0
25	11523.5
26	14961.0
27	18694.0
28	23260.5
29	28233.5
30	33203.5
31	38514.0
32	44192.0
33	50290.5
34	56952.5
35	64866.0
36	73442.5
37	81520.5
38	87525.0
39	89060.0
40	86329.0
41	81792.0
42	75613.5
43	69007.0
44	63788.0
45	58583.0
46	53148.0
47	47696.0
48	42019.0
49	35929.0
50	30673.5
51	26915.0
52	23475.0
53	20230.0
54	17213.0
55	14723.0
56	12690.0
57	10633.5
58	8630.0
59	7019.0
60	5635.5
61	4488.5
62	3556.0
63	2468.5
64	1638.0
65	1218.5
66	909.5
67	628.0
68	437.0
69	474.5
70	505.5
71	371.5
72	306.5
73	267.0
74	183.5
75	133.5
76	99.5
77	67.0
78	44.0
79	30.5
80	25.5
81	19.0
82	14.0
83	13.5
84	12.0
85	11.0
86	7.0
87	3.5
88	4.0
89	6.5
90	6.5
91	4.5
92	4.5
93	7.0
94	6.5
95	3.5
96	3.5
97	3.5
98	2.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03517569520227954
2	0.03138160741994957
3	0.028037665645692652
4	0.026430001331146053
5	0.032667738871586846
6	0.0019291971774559164
7	4.501460080730471E-4
8	0.003086715483929466
9	3.215328629093194E-4
10-14	0.007459562419496209
15-19	0.025221037766607012
20-24	0.02131119815362969
25-29	0.0021478395242342536
30-34	0.001890613233906798
35-39	0.005980511250113341
40-44	0.014224613855108289
45-49	0.008977197532428196
50-54	0.005003051346869009
55-59	0.0041413432742720335
60-64	0.011189343629244314
65-69	0.015137767185770756
70-74	0.019176219943911808
75-79	0.007330949274332482
80-84	0.011112175742146077
85-89	0.008925752274362707
90-94	0.009941796121156154
95-99	0.009491650113083108
100-101	0.005819744818658681
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1555051.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	25.314757851062303
#Duplication Level	Relative count
1	100.0
2	6.914944565503699
3	2.6346351859390342
4	1.786954335384689
5	1.3373721893632815
6	1.0560091951821078
7	0.8560618759129331
8	0.6728766073609348
9	0.5627259883623477
10++	3.9073537511074927
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GTCTC	358610	3.1784334	5.862412	90-94
TCTCT	536060	3.047688	4.5931954	90-94
GCTGC	216705	2.994286	7.4223633	90-94
CTGTC	332850	2.950117	5.5713983	95-97
GACGC	189735	2.68159	7.6639943	95-97
AGAAG	400345	2.657992	5.48445	5
CGCTG	186355	2.5749297	7.0592504	95-97
ACACA	417245	2.5386925	6.9634647	6
CTGCC	188740	2.4965377	6.9502096	90-94
GCCGA	174575	2.4673288	7.204704	95-97
CGACG	170445	2.408958	7.380651	95-97
TGCCG	165210	2.282762	6.8969812	90-94
CCGAC	168170	2.2753243	6.9561524	95-97
TACAC	367295	2.184808	8.601208	5
CTGAC	231350	2.097398	5.3309164	95-97
TGACG	217145	2.0564175	5.466091	95-97
CTTTG	333740	1.9820542	7.698453	9
AGAGA	294290	1.9538661	5.2321606	8
GACGA	200795	1.945069	5.475209	95-97
GAGAG	184465	1.8665783	6.4971104	7
TGGAG	172495	1.7064288	5.076875	5
TATAC	387100	1.542901	5.774964	5
GAGTC	144610	1.369493	6.718975	9
CCATG	150670	1.3659605	5.6539183	9
GTGTA	215065	1.3647333	10.093124	1
AAGAC	214635	1.3641735	5.703481	5
GTCTT	212470	1.2618418	6.3089566	1
TATGA	293690	1.2227966	5.5276303	4
GATTG	190215	1.207043	5.5515537	7
GACTT	196790	1.1954485	5.729906	7
GTGTT	191140	1.1857933	5.7447286	1
TCATA	296110	1.1802334	5.5487056	2
GTTCT	196975	1.1698183	5.1739206	1
ACACC	124515	1.1053593	5.1402063	6
TGGAC	114660	1.0858591	6.803239	5
GTCCA	117725	1.067284	7.6562524	1
AGACT	159645	0.99198204	5.2853346	6
GGACT	104360	0.9883154	6.2458973	6
GAGTA	146330	0.9497996	5.0565033	1
GTATA	180545	0.7517102	7.5715814	1
>>END_MODULE