##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-117_AGGCAGAA-GTAAGGAG_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1555051	
Filtered Sequences	0	
Sequence length	101	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.47884603141633	34.0	31.0	34.0	31.0	34.0
2	32.66877935193122	34.0	31.0	34.0	31.0	34.0
3	32.77747739463207	34.0	31.0	34.0	31.0	34.0
4	36.27777224026736	37.0	37.0	37.0	35.0	37.0
5	36.01043245526996	37.0	35.0	37.0	35.0	37.0
6	36.10163911022854	37.0	36.0	37.0	35.0	37.0
7	36.09624057346029	37.0	36.0	37.0	35.0	37.0
8	36.121326567424475	37.0	36.0	37.0	35.0	37.0
9	37.988793936661885	39.0	38.0	39.0	35.0	39.0
10-14	38.180009144394624	39.4	38.2	39.4	35.2	39.4
15-19	39.32709821092685	41.0	39.0	41.0	36.0	41.0
20-24	39.26026297529792	40.2	39.0	41.0	36.0	41.0
25-29	39.08002104111055	40.0	39.0	41.0	36.0	41.0
30-34	38.8905560010572	40.0	38.2	41.0	35.2	41.0
35-39	38.67972548810296	40.0	38.0	41.0	35.0	41.0
40-44	38.458558979737646	40.0	38.0	41.0	34.0	41.0
45-49	38.289310897198874	40.0	38.0	41.0	33.8	41.0
50-54	38.243053507569854	40.0	37.8	41.0	33.8	41.0
55-59	37.8706974883782	40.0	36.8	41.0	33.0	41.0
60-64	37.32566726107375	39.0	35.8	40.8	32.4	41.0
65-69	36.59467310075361	37.8	35.0	40.0	31.0	41.0
70-74	36.00822854041443	36.8	35.0	39.2	31.0	41.0
75-79	34.81400468537687	35.2	34.0	37.4	30.4	39.2
80-84	34.59022720155159	35.0	34.0	36.6	31.0	38.4
85-89	33.95667318949668	35.0	34.0	35.8	30.6	36.8
90-94	33.53875943618569	35.0	34.0	35.0	30.0	36.0
95-99	33.266047608727945	35.0	34.0	35.0	29.4	35.6
100-101	32.931477810052534	35.0	33.5	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	11.0
14	14.0
15	51.0
16	129.0
17	260.0
18	421.0
19	703.0
20	1104.0
21	1629.0
22	2256.0
23	3235.0
24	4331.0
25	5652.0
26	7117.0
27	9514.0
28	12391.0
29	15539.0
30	19773.0
31	25030.0
32	32459.0
33	44007.0
34	62401.0
35	99678.0
36	185901.0
37	377402.0
38	521640.0
39	122266.0
40	136.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.72676716069119	23.67632958661806	11.384578383602854	24.212324869087894
2	15.196045327462555	23.444465240005428	40.20410092750346	21.15538850502856
3	15.841088170098603	27.312416120114392	33.270355763251494	23.576139946535516
4	11.224647937591756	18.228083837764807	44.22221521995098	26.32505300469245
5	10.386733296850071	39.4353625701022	39.19311971118632	10.984784421861406
6	25.64243873673597	43.874895421436335	19.320266666495183	11.162399175332514
7	23.754204846014694	33.912649810199156	26.818348722967926	15.514796620818224
8	20.937834193219388	38.898659915333965	24.159915012433675	16.00359087901297
9	22.687037274018664	15.160917551900226	25.428876609191597	36.72316856488951
10-14	20.253753735408033	26.11100214719646	32.90632911718008	20.728915000215427
15-19	19.413049263121938	32.03976197553055	28.910118586520394	19.637070174827112
20-24	19.425149488039157	30.336721711739013	30.626862009022528	19.611266791199306
25-29	19.44167807872921	30.738706688939377	30.150703543043434	19.668911689287977
30-34	19.316022880697457	30.53456420472238	30.25290931697854	19.896503597601626
35-39	19.460716770347926	30.746712228178456	30.125242262119283	19.667328739354332
40-44	19.17143242437796	30.504096295499455	30.55511662488104	19.769354655241543
45-49	19.374403018866758	30.340442183273964	30.493683693515433	19.79147110434384
50-54	19.09464647781319	30.027996419155436	30.865843017513946	20.011514085517433
55-59	19.222654895494713	29.981066880937508	30.871174374476894	19.925103849090885
60-64	18.87940374870355	29.617666209424005	31.08133600644429	20.421594035428157
65-69	19.13631389361931	29.775464000962454	30.48574944945222	20.602472655966018
70-74	19.02168355912416	29.613674343910567	30.37631404060904	20.988328056356238
75-79	19.30417724900423	29.943894265068632	29.85774791736896	20.894180568558177
80-84	19.251624868412506	29.917218042646887	29.49381016289519	21.337346926045416
85-89	19.249795208924198	30.004551770245058	29.331239613340042	21.414413407490702
90-94	19.20168456859489	29.501236455548113	29.603538087936958	21.693540887920033
95-99	19.33724694643131	29.48533153922601	29.21410613880263	21.963315375540045
100-101	19.182180637278545	28.779460467509082	29.956400115751908	22.081958779460468
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	64.93909202977909
2	36.35143383249112
3	39.41722811663412
4	37.54970094228421
5	21.371517718711477
6	36.80483791206848
7	39.26900146683292
8	36.941425072232356
9	59.41020583890818
10-14	40.98266873562346
15-19	39.05011943794905
20-24	39.03641627923846
25-29	39.11058976801719
30-34	39.21252647829908
35-39	39.12804550970226
40-44	38.94078707961951
45-49	39.16587412321061
50-54	39.10616056333062
55-59	39.147758744585595
60-64	39.30099778413171
65-69	39.73878654958533
70-74	40.010011615480394
75-79	40.19835781756241
80-84	40.588971794457926
85-89	40.664208616414896
90-94	40.89522545651493
95-99	41.30056232197135
100-101	41.26413941673901
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	1.0
5	1.5
6	3.5
7	6.0
8	9.5
9	18.5
10	28.0
11	39.0
12	72.0
13	114.5
14	175.5
15	264.5
16	412.5
17	636.5
18	912.5
19	1335.5
20	1987.5
21	2971.5
22	4342.5
23	6154.0
24	8434.5
25	11135.0
26	14673.0
27	18646.5
28	23079.0
29	27788.5
30	32739.0
31	38281.5
32	43826.5
33	49676.5
34	56286.5
35	64222.5
36	73207.0
37	81218.0
38	87014.5
39	88776.0
40	86152.0
41	81706.0
42	75862.5
43	69866.0
44	64799.0
45	59294.0
46	54085.0
47	48975.0
48	42936.0
49	36635.5
50	31181.5
51	27116.5
52	23636.0
53	20441.5
54	17487.0
55	14824.0
56	12754.5
57	10650.0
58	8481.5
59	6937.5
60	5566.0
61	4438.0
62	3490.5
63	2341.5
64	1544.5
65	1169.0
66	845.0
67	566.5
68	410.5
69	407.5
70	451.0
71	379.5
72	320.5
73	288.0
74	208.5
75	149.0
76	88.0
77	45.0
78	16.5
79	6.5
80	4.0
81	1.5
82	0.0
83	0.5
84	0.5
85	0.5
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.014919124838992418
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.001131795677440804
20-24	6.559270403350115E-4
25-29	0.016938351218062947
30-34	0.006327766742055405
35-39	0.04113048382336013
40-44	0.04942603168642057
45-49	0.049670396662231664
50-54	0.05021057187191931
55-59	0.04064175387173797
60-64	0.05189540407356415
65-69	0.04437153508148607
70-74	0.003678335951682614
75-79	0.0014661898548664963
80-84	1.2861314516372774E-4
85-89	0.003318219145224176
90-94	0.002675153419405537
95-99	0.002212146096816117
100-101	6.430657258186387E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1555051.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	26.168452992707866
#Duplication Level	Relative count
1	100.0
2	7.114965109131886
3	2.6996733877285384
4	1.8524098200619479
5	1.4119050775553494
6	1.1003579477661407
7	0.9069215286900558
8	0.7243320116182372
9	0.5797066515613513
10++	4.130861846624805
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GTCTC	361390	3.2214935	5.924187	90-94
CCGAG	223375	3.1269069	7.941278	95-97
CTCCG	235205	3.0894644	7.589543	95-97
TCTCT	537185	3.0813515	4.615964	95-97
GAGCC	219640	3.0746229	7.4593725	95-97
CTGTC	334805	2.98451	5.6375947	95-97
CGAGC	207540	2.9052413	7.522722	95-97
TCTCC	330305	2.7918239	5.5777006	95-97
GCCCA	204170	2.7099676	7.1515684	90-94
AGCCC	202955	2.6938407	7.0022554	90-94
AGAAG	400475	2.6364267	5.2761126	5
ACACA	417705	2.4722562	7.073618	6
CCCAC	195360	2.4586658	6.638193	90-94
ATCTC	423170	2.4528315	5.6733766	95-97
CCACG	174930	2.3218622	6.675974	95-97
TCCGA	253065	2.2795477	5.4856787	95-97
TACAC	370350	2.1692054	9.164065	5
GAGAC	220050	2.112427	5.57779	95-97
CGAGA	211045	2.0259812	5.473837	95-97
CTTTG	334095	2.021136	8.057219	9
GGCAG	134205	1.9813322	6.561534	90-94
AGAGA	290720	1.9138821	5.1580143	8
ACGAG	196780	1.8890407	5.2535176	95-97
GAGAG	184805	1.8710365	6.4255323	7
CAGGC	133540	1.869355	6.2269597	90-94
TGGCT	167530	1.575005	5.0550075	6
TATAC	388290	1.5434374	5.7778373	5
TTGAG	233615	1.5061563	5.0170135	9
GAGTC	145600	1.3832039	6.3793364	9
GTCTT	226705	1.371471	6.1956577	1
CCATG	150050	1.3516138	5.847875	9
GACTC	149095	1.3430114	5.140365	7
AAGAC	214115	1.3365314	5.7502475	5
TATGA	294965	1.2365495	5.7338324	4
GATTG	191735	1.2361486	5.635934	7
GTGTT	192190	1.2262093	5.649402	1
GACTT	197375	1.2065719	5.912959	7
GTTCT	198760	1.2024155	5.1219783	1
TCATA	295275	1.1737062	5.7710185	2
GTATG	180965	1.1667125	5.1389213	3
GTGTA	171930	1.1084623	10.570179	1
TGGAC	115890	1.1009581	7.185389	5
ACCAT	186400	1.0917776	5.0122633	8
ACACC	122815	1.0599741	5.5361085	6
GTCCA	117110	1.0548983	7.264016	1
GGACT	104810	0.9956978	6.476063	6
AGACT	157155	0.97078884	5.4119725	6
GAGTA	148810	0.9694757	5.414814	1
ACACT	160260	0.938671	5.020783	6
GTATA	181565	0.761155	7.1597567	1
>>END_MODULE