##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-115_AGGCAGAA-TATCCTCT_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	2524992	
Filtered Sequences	0	
Sequence length	101	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.605103699338454	33.0	31.0	34.0	30.0	34.0
2	31.802515017869364	33.0	31.0	34.0	30.0	34.0
3	31.94177050857983	34.0	31.0	34.0	30.0	34.0
4	35.467212569386355	37.0	35.0	37.0	33.0	37.0
5	35.34213098496946	37.0	35.0	37.0	33.0	37.0
6	35.48649421463514	37.0	35.0	37.0	33.0	37.0
7	35.49903207614123	37.0	35.0	37.0	33.0	37.0
8	35.55235264111728	37.0	35.0	37.0	33.0	37.0
9	37.336692947938054	39.0	37.0	39.0	34.0	39.0
10-14	37.4596758326363	39.2	37.2	39.4	34.0	39.4
15-19	38.50519011545383	40.0	38.0	41.0	34.0	41.0
20-24	38.40247612665703	40.0	38.0	41.0	34.0	41.0
25-29	38.22416102704484	40.0	38.0	41.0	33.4	41.0
30-34	37.9692425956201	40.0	38.0	41.0	33.0	41.0
35-39	37.66346174562137	40.0	37.6	41.0	31.8	41.0
40-44	37.62345805452057	40.0	37.6	41.0	31.8	41.0
45-49	37.4743195225965	40.0	37.0	41.0	31.4	41.0
50-54	36.80725364674423	39.2	36.4	40.2	30.8	40.6
55-59	37.44456410158923	40.0	36.6	41.0	31.8	41.0
60-64	36.996119116417006	39.0	35.6	41.0	31.0	41.0
65-69	36.32606970635946	38.2	35.0	40.2	30.4	41.0
70-74	35.49732205092135	36.6	34.6	39.4	29.4	41.0
75-79	34.55045798164905	35.6	34.0	38.0	29.0	39.6
80-84	33.6352004283578	35.0	33.8	36.4	28.0	38.2
85-89	32.871722049020356	35.0	33.0	35.6	26.2	36.8
90-94	32.30685419993411	35.0	32.8	35.0	25.4	36.0
95-99	31.84077533710998	35.0	32.0	35.0	24.6	35.4
100-101	31.01937154652371	34.5	31.0	35.0	21.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	73.0
7	264.0
8	591.0
9	801.0
10	886.0
11	1177.0
12	1889.0
13	2650.0
14	3375.0
15	4085.0
16	4701.0
17	5416.0
18	6054.0
19	7079.0
20	8038.0
21	9073.0
22	10231.0
23	11811.0
24	13636.0
25	15737.0
26	18370.0
27	21725.0
28	25859.0
29	31231.0
30	38535.0
31	48128.0
32	62942.0
33	84406.0
34	119376.0
35	182622.0
36	317486.0
37	590366.0
38	725653.0
39	150638.0
40	87.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.42654353831702	23.68515823741416	10.79004012143797	24.098258102830844
2	14.845381736123656	23.750550679990745	40.36373403820372	21.04033354568188
3	15.575048261879076	28.09124614885457	33.069122737099235	23.26458285216712
4	11.035485436412436	18.51715839201355	44.54550316427489	25.901853007299124
5	10.509428221434446	39.306111744114	38.92621757092758	11.25824246352398
6	25.235519981623362	43.923927055836856	19.683280229866394	11.157272732673384
7	23.455195684718294	34.22862755348557	27.034986415733986	15.281190346062147
8	20.805735142919154	38.74568105812294	24.599235462136683	15.849348336821228
9	22.707229364192983	15.366893995199963	25.753748544542926	36.17212809606413
10-14	20.175418167761396	26.298118205467	33.03469865622316	20.49176497054844
15-19	19.396028071258492	32.066052294278236	29.0062461386491	19.53167349581417
20-24	19.362081071781883	30.454725041214363	30.629937098427806	19.553256788575947
25-29	19.366927921885143	30.884911885375104	30.204206169940623	19.54395402279913
30-34	19.23685161667258	30.637034765187398	30.27275758121344	19.85335603692658
35-39	19.38123695417653	30.886561111625554	30.2096734293385	19.52252850485942
40-44	19.121487046944893	30.676981727677127	30.588708832850603	19.612822392527374
45-49	19.288209826642937	30.56740187001765	30.49118163933699	19.65320666400242
50-54	19.08313290003197	30.27577890160544	30.826010692381274	19.815077505981314
55-59	19.294065759000265	30.248734559936096	30.8477699881389	19.60942969292474
60-64	19.028826314747988	29.904614140067302	31.07613520437869	19.990424340806012
65-69	19.34862127604155	30.064997812756424	30.640921782553608	19.945459128648423
70-74	19.337071680113876	29.921842345515522	30.605476232803593	20.135609741567013
75-79	19.56724820169957	30.24988983288423	30.266794138119263	19.916067827296935
80-84	19.684333904327705	30.17736156238256	30.067321567427086	20.070982965862648
85-89	19.78391487014906	30.156764738663515	29.982744527191883	20.076575863995537
90-94	19.89496893698469	29.793466159746902	30.283790148905698	20.02777475436271
95-99	20.196813321314707	29.751438935801826	29.827296017447765	20.224451725435706
100-101	20.369750967835714	29.183754420339238	30.370901538298845	20.075593073526203
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.52480164114786
2	35.88571528180554
3	38.8396311140462
4	36.93733844371156
5	21.767670684958425
6	36.392792714296746
7	38.73638603078044
8	36.655083479740384
9	58.87935746025711
10-14	40.66718313830984
15-19	38.92770156707267
20-24	38.91533786035783
25-29	38.91088194468427
30-34	39.090207653599165
35-39	38.903765459035945
40-44	38.73430943947227
45-49	38.94141649064536
50-54	38.898210406013284
55-59	38.903495451925004
60-64	39.019250655553996
65-69	39.29408040468997
70-74	39.47268142168089
75-79	39.483316028996505
80-84	39.755316870190356
85-89	39.8604907341446
90-94	39.92274369134739
95-99	40.421265046750406
100-101	40.44534404136192
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	4.5
2	10.0
3	18.0
4	19.5
5	24.0
6	40.5
7	47.5
8	59.0
9	77.5
10	108.5
11	147.5
12	205.5
13	299.0
14	439.5
15	622.0
16	894.0
17	1326.5
18	1900.0
19	2705.5
20	3861.0
21	5658.0
22	8223.5
23	11494.0
24	15372.0
25	20050.0
26	25845.5
27	32445.0
28	39764.0
29	47458.0
30	56114.5
31	65361.0
32	74737.0
33	85073.5
34	96427.0
35	109211.0
36	123848.0
37	136412.0
38	144348.5
39	145618.5
40	140214.0
41	131833.5
42	121310.0
43	110475.0
44	100970.0
45	91934.0
46	83406.5
47	74277.5
48	64260.0
49	54764.5
50	46428.5
51	40141.0
52	34818.5
53	30363.5
54	26153.0
55	22252.0
56	19235.0
57	16190.5
58	13187.0
59	10732.0
60	8571.5
61	6781.5
62	5301.5
63	3748.5
64	2490.0
65	1805.0
66	1348.5
67	920.5
68	669.5
69	730.0
70	731.5
71	578.5
72	506.0
73	431.0
74	318.0
75	203.5
76	141.0
77	108.5
78	72.0
79	49.5
80	35.5
81	25.0
82	23.0
83	20.5
84	16.5
85	15.0
86	16.0
87	14.0
88	11.5
89	9.0
90	10.0
91	11.0
92	9.5
93	9.5
94	10.0
95	11.0
96	7.5
97	6.0
98	6.0
99	2.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.035841697716269987
2	0.033267432134438446
3	0.029425835804628292
4	0.028396129571895676
5	0.03437634653891972
6	0.0018613920360935799
7	4.7524903049197783E-4
8	0.002693077839454541
9	4.7524903049197783E-4
10-14	0.006653486426887689
15-19	0.024411958532937927
20-24	0.02012679644133526
25-29	0.0021227790028641672
30-34	0.001845550401743847
35-39	0.005615859376980205
40-44	0.012720832382835272
45-49	0.008039629432489292
50-54	0.005101006260613895
55-59	0.003944566953083415
60-64	0.010186170886878057
65-69	0.01398816313081388
70-74	0.017964413352596763
75-79	0.007580222036347046
80-84	0.010835677895217093
85-89	0.009021810762172712
90-94	0.010027754543380733
95-99	0.009671317770511749
100-101	0.006237643525207209
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2524992.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	28.29232898431935
#Duplication Level	Relative count
1	100.0
2	8.319123553257006
3	2.697047488772712
4	1.8319685698402883
5	1.4478360534912336
6	1.2011167950231416
7	1.01373507973092
8	0.8838170904616461
9	0.7189211810044909
10++	6.807577716566417
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AGAAG	665905	2.6036909	5.102065	5
GCTGC	294445	2.5985312	5.763645	90-94
CTCTT	691340	2.529784	5.0465713	1
ACACA	594850	2.2493794	7.291021	6
CTGCC	251000	2.1783943	5.388594	90-94
GACGC	239305	2.135158	5.5032587	95-97
CGCTG	238170	2.101894	5.324913	95-97
GCCGA	233145	2.0801964	5.4497523	90-94
CTTTG	553505	2.0595589	8.277072	9
CGACG	230350	2.0552583	5.6359715	95-97
CCGAC	224695	1.9715631	5.2219205	95-97
TACAC	514745	1.9252758	8.995472	5
TGCCG	214385	1.891987	5.177265	90-94
GAGAG	299650	1.7997115	5.9009347	7
GCTTT	466445	1.7356138	5.26703	1
TTGAG	385550	1.4748533	5.029131	9
GACTC	251100	1.4426433	5.3772755	7
GAGTC	242430	1.4163139	6.8902035	9
CCATG	239400	1.3754233	5.6113453	9
AAGAC	353760	1.3602694	5.674035	5
TATAC	538535	1.3188932	5.873416	5
GTCTT	353070	1.3137523	6.5612245	1
GTGTA	335705	1.2841802	10.1003	1
GACTT	330485	1.2432513	5.9837546	7
GTGTT	324160	1.2265157	5.8550706	1
GATTG	319870	1.223606	5.65426	7
TATGA	489130	1.2180945	5.492856	4
GTTCT	325500	1.2111661	5.470997	1
TCATA	492900	1.2071313	5.6084948	2
ACACC	198985	1.1366483	5.343487	6
TGGAC	193815	1.1322975	7.3087163	5
ACATG	294835	1.1213489	5.1127496	8
GTCCA	194675	1.1184652	7.851035	1
ACACT	275820	1.031636	5.047783	6
GGACT	174275	1.0181419	6.7512465	6
AGACT	259110	0.9854756	5.2841244	6
GTATG	247715	0.9475899	5.043455	3
GTATA	298255	0.74275297	7.099702	1
>>END_MODULE