FastQC Report![[OK]](Icons/tick.png)
Basic Statistics
| Measure | Value |
|---|---|
| Filename | Nextera-115_AGGCAGAA-TATCCTCT_L005_R1_001.fastq.gz |
| File type | Conventional base calls |
| Encoding | Sanger / Illumina 1.9 |
| Total Sequences | 2524992 |
| Filtered Sequences | 0 |
| Sequence length | 101 |
| %GC | 39 |
Per base sequence quality
Per sequence quality scores
![[FAIL]](Icons/error.png)
Per base sequence content
Per base GC content
Per sequence GC content
Per base N content
Sequence Length Distribution
![[WARN]](Icons/warning.png)
Sequence Duplication Levels
Overrepresented sequences
No overrepresented sequences
Kmer Content
| Sequence | Count | Obs/Exp Overall | Obs/Exp Max | Max Obs/Exp Position |
|---|---|---|---|---|
| AGAAG | 665300 | 2.6573644 | 5.0816426 | 5 |
| CTCCG | 310645 | 2.6189892 | 5.754383 | 95-97 |
| CCGAG | 294665 | 2.6121647 | 5.8271275 | 95-97 |
| GAGCC | 293515 | 2.60197 | 5.8271275 | 95-97 |
| CGAGC | 272450 | 2.4152312 | 5.6551933 | 95-97 |
| GCCCA | 271370 | 2.3054287 | 5.3831644 | 90-94 |
| AGCCC | 268760 | 2.2832556 | 5.2472267 | 90-94 |
| ACACA | 597080 | 2.1902916 | 6.929391 | 6 |
| CCCAC | 260590 | 2.1216097 | 5.0641365 | 90-94 |
| CTTTG | 556555 | 2.0820827 | 8.176745 | 9 |
| CCACG | 231005 | 1.9625072 | 5.053617 | 90-94 |
| TACAC | 516590 | 1.8805931 | 8.708727 | 5 |
| GAGAG | 297275 | 1.8458366 | 6.2618823 | 7 |
| TTGAG | 385945 | 1.5181614 | 5.004527 | 9 |
| GACTC | 252710 | 1.4301203 | 5.1721153 | 7 |
| GAGTC | 240225 | 1.4185691 | 6.9144254 | 9 |
| GTCTT | 369945 | 1.3839712 | 6.2530646 | 1 |
| CCATG | 244450 | 1.3833759 | 5.8580723 | 9 |
| AAGAC | 354745 | 1.3578999 | 5.6345587 | 5 |
| TATAC | 539485 | 1.3082433 | 5.8646526 | 5 |
| GTGTT | 325590 | 1.2709926 | 6.051686 | 1 |
| GATTG | 318335 | 1.2522093 | 5.8150926 | 7 |
| GACTT | 330940 | 1.247555 | 6.01513 | 7 |
| TATGA | 490855 | 1.2420653 | 5.577343 | 4 |
| GTTCT | 324570 | 1.2142226 | 5.2716584 | 1 |
| TCATA | 496965 | 1.205133 | 5.6066976 | 2 |
| GTATG | 290070 | 1.1410254 | 5.1761765 | 3 |
| TGGAC | 192430 | 1.1363316 | 7.521406 | 5 |
| GTGTA | 287700 | 1.1317028 | 10.1324005 | 1 |
| ACATG | 296700 | 1.1270639 | 5.0667357 | 8 |
| GTCCA | 196150 | 1.1100397 | 7.403786 | 1 |
| ACACC | 198295 | 1.0836784 | 5.0715094 | 6 |
| GGACT | 174765 | 1.0320166 | 6.897246 | 6 |
| AGACT | 260145 | 0.98820364 | 5.3448443 | 6 |
| GTATA | 300200 | 0.75962967 | 7.170727 | 1 |