##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-115_AGGCAGAA-TATCCTCT_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	2524992	
Filtered Sequences	0	
Sequence length	101	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.49101224875168	34.0	31.0	34.0	31.0	34.0
2	32.67935739994424	34.0	31.0	34.0	31.0	34.0
3	32.78676605708058	34.0	31.0	34.0	31.0	34.0
4	36.28362545307074	37.0	37.0	37.0	35.0	37.0
5	36.01736678769675	37.0	35.0	37.0	35.0	37.0
6	36.10841420487669	37.0	37.0	37.0	35.0	37.0
7	36.10574805781563	37.0	36.0	37.0	35.0	37.0
8	36.13168516969559	37.0	37.0	37.0	35.0	37.0
9	37.99928475020911	39.0	38.0	39.0	35.0	39.0
10-14	38.19187213266418	39.4	38.2	39.4	35.2	39.4
15-19	39.34199229146073	41.0	39.0	41.0	36.0	41.0
20-24	39.27897553734824	40.2	39.0	41.0	36.0	41.0
25-29	39.095862719565055	40.0	39.0	41.0	36.0	41.0
30-34	38.909618089879096	40.0	38.2	41.0	35.4	41.0
35-39	38.69586002648721	40.0	38.0	41.0	35.0	41.0
40-44	38.47758955275898	40.0	38.0	41.0	34.2	41.0
45-49	38.31390673713025	40.0	38.0	41.0	34.0	41.0
50-54	38.262927803335614	40.0	37.8	41.0	34.0	41.0
55-59	37.89369154436925	40.0	37.0	41.0	33.0	41.0
60-64	37.34940284959318	39.0	35.8	40.8	32.6	41.0
65-69	36.619078555496415	38.0	35.0	40.0	31.2	41.0
70-74	36.0335147992548	36.8	35.0	39.2	31.0	41.0
75-79	34.84136971523078	35.2	34.0	37.4	30.4	39.2
80-84	34.6181659981497	35.0	34.0	36.6	31.0	38.4
85-89	33.9849007046359	35.0	34.0	35.8	30.8	36.8
90-94	33.56664567650115	35.0	34.0	35.0	30.0	36.0
95-99	33.29542176767293	35.0	34.0	35.0	29.6	35.8
100-101	32.9614856205485	35.0	33.5	35.0	29.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
12	5.0
13	7.0
14	30.0
15	69.0
16	181.0
17	364.0
18	640.0
19	1165.0
20	1779.0
21	2563.0
22	3662.0
23	4935.0
24	6884.0
25	8999.0
26	11757.0
27	15231.0
28	19450.0
29	25061.0
30	31322.0
31	39753.0
32	52217.0
33	69963.0
34	100666.0
35	160468.0
36	300425.0
37	613552.0
38	855192.0
39	198437.0
40	215.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.73050528476922	23.785421894406003	11.169777963652955	24.314294857171824
2	15.084745964114814	23.63559017579772	40.20699000223	21.072673857857467
3	15.719812181583148	27.718186829898865	33.354798747877226	23.20720224064076
4	11.19900577902821	18.484415000126734	44.25994220971789	26.056637011127165
5	10.332032735153222	39.32182755430512	39.10578726586064	11.240352444681013
6	25.212119483942917	44.14013984994804	19.51356677565711	11.13417389045193
7	23.387915684485336	34.191316249714845	27.048085696905176	15.372682368894633
8	20.7611746888703	38.91548963323448	24.45342401084835	15.869911667046866
9	22.574645781055942	15.22131555268294	25.72332902440879	36.480709641852336
10-14	20.109180543938358	26.21461770967987	33.07446518642435	20.601736559957416
15-19	19.32035313609595	32.10777404289534	28.994613072137813	19.577259748870894
20-24	19.300881458651176	30.42452955353438	30.667391479468026	19.607197508346424
25-29	19.327394233350034	30.87172184148225	30.212445935081856	19.588437990085865
30-34	19.210672423280986	30.639490998438145	30.27407051298046	19.875766065300404
35-39	19.36198783578034	30.823342420231835	30.22889570331437	19.585774040673446
40-44	19.099211231120407	30.618040659176593	30.598331739396567	19.684416370306433
45-49	19.245628570873833	30.518786377394928	30.55413106937109	19.681453982360146
50-54	19.031984121185168	30.223740020068828	30.848604604609047	19.895671254136957
55-59	19.201047623492638	30.198582370672884	30.86467052848139	19.735699477353087
60-64	18.91222598630428	29.89595426295567	30.997711039749674	20.194108710990378
65-69	19.210075484673062	30.084814764715517	30.503424771242777	20.201684979368643
70-74	19.10062750252664	29.933238496506515	30.462321213583976	20.503812787382873
75-79	19.2948547597878	30.30263905693369	30.029842115998107	20.372664067280407
80-84	19.24219837876457	30.23980311985641	29.785773239318363	20.732225262060652
85-89	19.26168029011392	30.276553048431477	29.68339960892096	20.778367052533643
90-94	19.19122358313113	29.81421030965529	30.05774971521985	20.936816391993727
95-99	19.285183748550754	29.844357924257984	29.68129624922503	21.18916207796623
100-101	19.171236649952416	29.198876352430563	30.42395002596049	21.205936971656534
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.04480014194104
2	36.15741982197228
3	38.92701442222391
4	37.25564279015538
5	21.57238517983423
6	36.34629337439485
7	38.760598053379965
8	36.63108635591717
9	59.05535542290827
10-14	40.710917103895774
15-19	38.89761288496684
20-24	38.9080789669976
25-29	38.9158322234359
30-34	39.08643848858139
35-39	38.94776187645378
40-44	38.78362760142684
45-49	38.92708255323398
50-54	38.927655375322125
55-59	38.93674710084573
60-64	39.106334697294656
65-69	39.411760464041706
70-74	39.60444028990951
75-79	39.66751882706821
80-84	39.97442364082522
85-89	40.04004734264756
90-94	40.12803997512486
95-99	40.47434582651698
100-101	40.37717362160895
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	1.0
4	1.5
5	2.0
6	6.5
7	11.0
8	17.5
9	31.0
10	53.0
11	80.0
12	131.0
13	217.5
14	334.0
15	509.5
16	803.0
17	1245.5
18	1814.0
19	2586.0
20	3827.0
21	5726.5
22	8326.5
23	11681.5
24	15560.0
25	20105.5
26	25708.0
27	32178.0
28	39689.0
29	47475.0
30	55765.5
31	64863.5
32	73971.0
33	83935.0
34	95191.5
35	108203.5
36	123015.5
37	135865.0
38	143413.5
39	144864.5
40	139656.5
41	130675.0
42	120238.0
43	109766.0
44	101354.0
45	92845.5
46	84458.0
47	75956.5
48	66294.0
49	56321.0
50	47584.5
51	41273.5
52	35796.5
53	31025.0
54	26560.0
55	22648.5
56	19612.0
57	16469.5
58	13360.5
59	10829.0
60	8647.5
61	6841.5
62	5325.5
63	3714.5
64	2423.0
65	1676.5
66	1226.5
67	883.5
68	631.0
69	650.5
70	686.0
71	566.0
72	506.5
73	443.5
74	330.5
75	237.0
76	127.0
77	64.0
78	30.5
79	14.0
80	11.5
81	7.5
82	5.0
83	3.0
84	1.5
85	1.5
86	1.0
87	1.5
88	0.5
89	0.5
90	0.5
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.01302974425265506
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0011009935873064153
20-24	7.20794362912833E-4
25-29	0.017204014903809597
30-34	0.006914873393658277
35-39	0.0421862722733379
40-44	0.049964514739056595
45-49	0.050495209489772645
50-54	0.05058233847869617
55-59	0.04086349580513522
60-64	0.052016006387346964
65-69	0.04417439738422933
70-74	0.0037148632550122926
75-79	0.0014653511773502648
80-84	1.5841634349732594E-4
85-89	0.003184168504296251
90-94	0.002447532507033686
95-99	0.0022891161635363597
100-101	3.9604085874331486E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	2524992.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	29.056874743546285
#Duplication Level	Relative count
1	100.0
2	8.613568208822919
3	2.761170102412252
4	1.8728428358574905
5	1.5107221666886705
6	1.197009989752488
7	1.076303100029548
8	0.8543784538201844
9	0.7732785122875842
10++	7.0770700917246625
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AGAAG	665300	2.6573644	5.0816426	5
CTCCG	310645	2.6189892	5.754383	95-97
CCGAG	294665	2.6121647	5.8271275	95-97
GAGCC	293515	2.60197	5.8271275	95-97
CGAGC	272450	2.4152312	5.6551933	95-97
GCCCA	271370	2.3054287	5.3831644	90-94
AGCCC	268760	2.2832556	5.2472267	90-94
ACACA	597080	2.1902916	6.929391	6
CCCAC	260590	2.1216097	5.0641365	90-94
CTTTG	556555	2.0820827	8.176745	9
CCACG	231005	1.9625072	5.053617	90-94
TACAC	516590	1.8805931	8.708727	5
GAGAG	297275	1.8458366	6.2618823	7
TTGAG	385945	1.5181614	5.004527	9
GACTC	252710	1.4301203	5.1721153	7
GAGTC	240225	1.4185691	6.9144254	9
GTCTT	369945	1.3839712	6.2530646	1
CCATG	244450	1.3833759	5.8580723	9
AAGAC	354745	1.3578999	5.6345587	5
TATAC	539485	1.3082433	5.8646526	5
GTGTT	325590	1.2709926	6.051686	1
GATTG	318335	1.2522093	5.8150926	7
GACTT	330940	1.247555	6.01513	7
TATGA	490855	1.2420653	5.577343	4
GTTCT	324570	1.2142226	5.2716584	1
TCATA	496965	1.205133	5.6066976	2
GTATG	290070	1.1410254	5.1761765	3
TGGAC	192430	1.1363316	7.521406	5
GTGTA	287700	1.1317028	10.1324005	1
ACATG	296700	1.1270639	5.0667357	8
GTCCA	196150	1.1100397	7.403786	1
ACACC	198295	1.0836784	5.0715094	6
GGACT	174765	1.0320166	6.897246	6
AGACT	260145	0.98820364	5.3448443	6
GTATA	300200	0.75962967	7.170727	1
>>END_MODULE