##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-114_AGGCAGAA-CTCTCTAT_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1570776	
Filtered Sequences	0	
Sequence length	101	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.385813126760276	31.0	31.0	34.0	28.0	34.0
2	31.5762056461265	33.0	31.0	34.0	28.0	34.0
3	31.711981848462163	33.0	31.0	34.0	29.0	34.0
4	35.2478768455846	37.0	35.0	37.0	32.0	37.0
5	35.120528324853446	37.0	35.0	37.0	32.0	37.0
6	35.27587701874742	37.0	35.0	37.0	32.0	37.0
7	35.27611448099538	37.0	35.0	37.0	32.0	37.0
8	35.32308489561847	37.0	35.0	37.0	32.0	37.0
9	37.07752855913255	39.0	37.0	39.0	33.0	39.0
10-14	37.16928626360474	39.2	37.2	39.4	33.0	39.4
15-19	38.1686477257101	40.0	38.0	41.0	33.0	41.0
20-24	38.05041520878852	40.0	38.0	41.0	32.8	41.0
25-29	37.85112644960198	40.0	38.0	41.0	32.0	41.0
30-34	37.56956765318544	40.0	37.2	41.0	31.4	41.0
35-39	37.24453429387768	40.0	37.0	41.0	30.6	41.0
40-44	37.184355630592776	40.0	36.8	41.0	30.4	41.0
45-49	37.00311998655442	39.8	36.4	41.0	30.0	41.0
50-54	36.359012488095054	38.6	35.4	40.0	29.6	40.6
55-59	37.00936174222168	39.6	36.0	41.0	30.4	41.0
60-64	36.553610954076206	39.0	35.0	41.0	29.6	41.0
65-69	35.87145334535287	37.6	34.8	40.0	29.0	41.0
70-74	35.03433984221812	36.4	34.0	39.2	28.0	40.8
75-79	34.07849992615115	35.2	33.8	37.6	27.2	39.4
80-84	33.15882022643585	35.0	33.0	36.4	26.0	38.0
85-89	32.39680972971321	35.0	32.2	35.4	25.2	36.6
90-94	31.817295273164348	35.0	31.8	35.0	24.4	36.0
95-99	31.349929334290824	34.0	31.0	35.0	22.2	35.0
100-101	30.49439289879652	34.0	30.0	35.0	19.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	3.0
6	51.0
7	160.0
8	367.0
9	463.0
10	613.0
11	918.0
12	1617.0
13	2318.0
14	2989.0
15	3542.0
16	3978.0
17	4473.0
18	4997.0
19	5715.0
20	6320.0
21	7210.0
22	8051.0
23	8970.0
24	10327.0
25	11859.0
26	13621.0
27	16092.0
28	18963.0
29	22809.0
30	27189.0
31	34342.0
32	44585.0
33	58968.0
34	81516.0
35	122723.0
36	205890.0
37	358885.0
38	405778.0
39	74423.0
40	51.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.3834463504226	22.995878400065212	10.214206018860523	24.406469230651663
2	14.82369018992501	23.85493509537007	40.23380186256929	21.08757285213564
3	15.422470544933068	28.233753067771804	32.939941083498155	23.403835303796967
4	10.819149335858423	18.65856073684405	44.71358078622085	25.808709141076676
5	10.321376828899396	39.326360032478384	38.977886039069595	11.374377099552627
6	24.89158059324451	44.405354887340586	19.62932404351546	11.073740475899442
7	23.59477885644402	34.16458328956491	27.0068062290588	15.233831624932279
8	20.900685930092937	38.94719216865706	24.436657469150973	15.715464432099033
9	23.08003277375604	14.800011968659938	24.789641252151014	37.33031400543301
10-14	20.336173253645104	25.893996795221152	33.309058950205724	20.46077100092802
15-19	19.686049402338483	31.750411618230206	28.734926010604262	19.82861296882705
20-24	19.57905780285427	30.048131476335037	30.51994576835508	19.85286495245562
25-29	19.604955776911858	30.78327509965681	29.770835489959325	19.84093363347201
30-34	19.40343300478768	30.4644955379647	29.88438702100143	20.24768443624619
35-39	19.69134830174461	30.821430713362634	29.637978944870003	19.84924204002275
40-44	19.26894958619908	30.590947698976546	30.233597909538112	19.90650480528626
45-49	19.579798033305252	30.35024300996183	30.110467408345365	19.959491548387557
50-54	19.338561161064163	29.97715027150367	30.556791029714837	20.127497537717332
55-59	19.5922178956618	29.97995685267198	30.68231375923037	19.745511492435845
60-64	19.27147984945348	29.463753159943707	31.04069431915597	20.224072671446844
65-69	19.665244259550054	29.744362763606837	30.453868381518546	20.13652459532456
70-74	19.561949761263676	29.545359901390494	30.49025729373756	20.402433043608266
75-79	19.7933748227168	30.137469130630436	29.930179260724284	20.138976785928485
80-84	19.881724724043032	30.099989354435657	29.703594889925373	20.314691031595935
85-89	20.04788382988634	30.144006760070102	29.5320502935565	20.276059116487055
90-94	20.05914324428569	29.723752431671574	30.101015683286818	20.116088640755915
95-99	20.461331352990072	29.78202217087999	29.410440402262317	20.346206073867616
100-101	20.582325545860563	28.90197588950044	30.444137430484155	20.071561134154845
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	66.78991558107427
2	35.91126304206065
3	38.82630584873004
4	36.627858476935096
5	21.69575392845202
6	35.965321069143954
7	38.828610481376295
8	36.61615036219197
9	60.41034677918905
10-14	40.79694425457313
15-19	39.514662371165535
20-24	39.431922755309884
25-29	39.44588941038386
30-34	39.65111744103387
35-39	39.54059034176736
40-44	39.175454391485346
45-49	39.53928958169281
50-54	39.466058698781495
55-59	39.33772938809765
60-64	39.495552520900326
65-69	39.80176885487462
70-74	39.964382804871946
75-79	39.932351608645284
80-84	40.19641575563897
85-89	40.3239429463734
90-94	40.175231885041605
95-99	40.80753742685769
100-101	40.653886680015404
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	3.0
2	2.5
3	4.5
4	8.0
5	16.5
6	23.5
7	26.5
8	29.0
9	36.0
10	53.5
11	79.5
12	126.5
13	188.5
14	256.5
15	358.0
16	504.0
17	744.5
18	1072.5
19	1472.5
20	2057.0
21	2895.5
22	4026.0
23	5636.5
24	7633.5
25	9968.0
26	12977.0
27	16525.5
28	20659.0
29	25358.0
30	30511.5
31	35972.0
32	41734.5
33	48561.5
34	56903.0
35	66846.5
36	78598.0
37	90379.0
38	98595.5
39	99837.0
40	94422.5
41	86427.5
42	77067.5
43	68330.0
44	62082.5
45	56120.0
46	50569.5
47	45322.0
48	39580.0
49	33774.0
50	28804.5
51	25316.5
52	22320.0
53	19557.5
54	16928.5
55	14507.0
56	12798.5
57	11117.0
58	9249.0
59	7523.0
60	6040.0
61	4934.0
62	3913.5
63	2811.0
64	1974.5
65	1550.0
66	1193.0
67	883.0
68	682.5
69	714.0
70	738.0
71	603.0
72	500.5
73	426.5
74	340.5
75	254.5
76	176.0
77	129.0
78	88.0
79	57.5
80	46.5
81	35.5
82	24.5
83	22.0
84	21.0
85	14.0
86	11.0
87	11.5
88	11.5
89	9.5
90	6.0
91	7.0
92	6.0
93	5.5
94	5.5
95	6.5
96	8.5
97	8.0
98	5.0
99	2.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03259535414342974
2	0.030048842100974298
3	0.026611050843659438
4	0.025656108827738645
5	0.0318950633317545
6	0.0015279072254732692
7	5.72965209552476E-4
8	0.003947093665805946
9	4.456396074297035E-4
10-14	0.007741396609064565
15-19	0.02475209705266696
20-24	0.020855933627710124
25-29	0.002253663157573072
30-34	0.002253663157573072
35-39	0.0063280824255017906
40-44	0.013623839427136651
45-49	0.00896372238944318
50-54	0.005029361283849511
55-59	0.004112616948565549
60-64	0.011357443709351302
65-69	0.015648316500888732
70-74	0.019009712396929924
75-79	0.007970582692885555
80-84	0.010746280819161994
85-89	0.009001920070080011
90-94	0.009460292237721992
95-99	0.008861861907744961
100-101	0.0057933148965861455
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1570776.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	28.375533848843574
#Duplication Level	Relative count
1	100.0
2	7.383006758192715
3	2.8963683063228265
4	1.8926582831883125
5	1.450612973544074
6	1.1202934015022254
7	0.8956275161061151
8	0.7104299619282405
9	0.5774520459775698
10++	4.514569886270485
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATATGGACTTTGGCTACACCATGAAAGCTTTGAGAAGCAAGAAGAAGGT	1857	0.11822182157099421	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CAAAG	406300	2.5007725	5.177008	4
CTTTG	397305	2.3851721	10.937837	9
GCTGC	173360	2.3731558	5.0736556	90-94
ACACA	379415	2.2991896	8.310715	6
GAGAG	231230	2.1817372	5.9851036	7
TACAC	334550	2.0105307	10.185328	5
GCTTT	333785	2.0038376	5.96383	1
CGACG	139700	1.9283432	5.023659	95-97
CACAT	314160	1.8879938	6.0474653	7
GCCAA	203230	1.8587112	5.802718	1
TTGGC	194885	1.7805268	5.154093	5
TGGCT	194075	1.7731262	5.7199864	6
GACTC	188000	1.7051846	6.3428497	7
GAGTC	177880	1.6387314	9.864481	9
TTGAG	264265	1.624849	5.9634547	9
ATACA	398420	1.6113654	5.825163	6
AAGAC	246105	1.514774	6.4100566	5
CATGA	245090	1.4960372	5.677537	9
CCATG	164550	1.49249	5.79727	9
ATGGA	239440	1.4845012	5.4974647	4
GTGTA	237445	1.4599445	11.545058	1
GTCTT	241375	1.4490653	7.24221	1
TGTGT	236560	1.4424609	5.550542	8
GACTT	237680	1.4387947	8.263998	7
TCCAT	240415	1.4328499	5.5071497	2
TATGA	348775	1.4208716	7.0092754	4
TCATA	349100	1.4002069	7.075847	2
ACTCA	232245	1.395713	5.866808	8
TGAGT	225995	1.3895435	7.311427	8
GTGTT	221985	1.3535877	6.415374	1
AACAC	221745	1.3437365	5.5320015	5
ATGAG	214575	1.3303412	6.0825458	7
TATAC	324965	1.3034039	6.4903913	5
GATTG	209285	1.2868011	6.56593	7
ACACC	139540	1.2564803	5.5763063	6
GTTCT	206925	1.242249	5.6784515	1
TGGAC	130445	1.2017332	8.781033	5
GTCCA	129925	1.1784366	9.058776	1
ACATG	192295	1.1737748	6.3467293	8
ACACT	191760	1.1524118	5.5111203	6
ATGTG	186615	1.1474133	5.549135	7
GGACT	123030	1.1334221	8.653843	6
AGACT	176000	1.0743096	6.260742	6
ACTTT	267085	1.0623835	5.04057	8
GTATG	170295	1.0470688	6.1018953	3
TGTAT	258165	1.0430287	5.4009585	2
GAGTA	164350	1.0189518	5.2568426	1
CATAC	167480	1.0064975	5.859109	3
ATACT	200930	0.8059111	5.302332	6
GTATA	196015	0.7985438	7.6731997	1
>>END_MODULE