##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-114_AGGCAGAA-CTCTCTAT_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1570776	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.437298507234644	34.0	31.0	34.0	31.0	34.0
2	32.620565885906075	34.0	31.0	34.0	31.0	34.0
3	32.725355493081125	34.0	31.0	34.0	31.0	34.0
4	36.237145207209686	37.0	35.0	37.0	35.0	37.0
5	35.971205951707944	37.0	35.0	37.0	35.0	37.0
6	36.06967065959755	37.0	36.0	37.0	35.0	37.0
7	36.05929871604863	37.0	36.0	37.0	35.0	37.0
8	36.08754972064763	37.0	36.0	37.0	35.0	37.0
9	37.94024927806384	39.0	38.0	39.0	35.0	39.0
10-14	38.117290180140266	39.2	38.2	39.4	35.2	39.4
15-19	39.24449864270908	40.0	39.0	41.0	36.0	41.0
20-24	39.175807244317454	40.0	39.0	41.0	36.0	41.0
25-29	38.98232720642536	40.0	38.8	41.0	35.8	41.0
30-34	38.78741781132383	40.0	38.0	41.0	35.0	41.0
35-39	38.559203221847035	40.0	38.0	41.0	34.4	41.0
40-44	38.32741485736987	40.0	38.0	41.0	33.8	41.0
45-49	38.145221979454746	40.0	38.0	41.0	33.4	41.0
50-54	38.08538836855159	40.0	37.4	41.0	33.2	41.0
55-59	37.7102746667889	39.6	36.6	41.0	33.0	41.0
60-64	37.15746942912293	39.0	35.6	40.6	31.8	41.0
65-69	36.41463887912725	37.8	35.0	40.0	31.0	41.0
70-74	35.83175156737816	36.6	35.0	39.2	31.0	40.8
75-79	34.6657028118586	35.2	33.6	37.4	30.2	39.2
80-84	34.460444519142136	35.0	34.0	36.6	31.0	38.2
85-89	33.828174227260924	35.0	34.0	35.6	30.0	36.8
90-94	33.406927658685895	35.0	34.0	35.0	29.4	36.0
95-99	33.13561233428573	35.0	34.0	35.0	29.0	35.6
100-101	32.78916981160904	35.0	33.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	2.0
13	8.0
14	14.0
15	38.0
16	133.0
17	262.0
18	485.0
19	759.0
20	1164.0
21	1650.0
22	2449.0
23	3447.0
24	4639.0
25	6024.0
26	7953.0
27	10489.0
28	13276.0
29	16969.0
30	21540.0
31	27102.0
32	35190.0
33	47999.0
34	68231.0
35	108851.0
36	196415.0
37	382317.0
38	507245.0
39	106001.0
40	123.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.550545717530696	23.144484000264836	10.628377311596306	24.67659297060816
2	15.03105843161924	23.779947382763652	40.00858277282139	21.180411412795717
3	15.505393512505922	27.844963253831228	33.25872053048939	23.390922703173462
4	11.078727966304552	18.706104498668175	44.46114531925621	25.754022215771062
5	10.1321257773228	39.4141494395127	39.13524270806277	11.318482075101734
6	24.821998808232365	44.59891162075306	19.49214910337311	11.086940467641472
7	23.40607444982607	34.24988668021411	27.00843404788461	15.335604822075203
8	20.854660371688897	39.114042995309326	24.317280121417696	15.714016511584084
9	22.990420021696284	14.643781162941119	24.658640060708848	37.707158754653754
10-14	20.219038233331805	25.851375371154127	33.37778270103439	20.55180369447967
15-19	19.583256035206436	31.85786329175299	28.695479673446872	19.8634009995937
20-24	19.509240526943106	30.049745175186573	30.51480507528368	19.926209222586643
25-29	19.587770392601506	30.83494120420242	29.708105057270174	19.869183345925897
30-34	19.347875968921176	30.431058347028173	29.92958903251388	20.29147665153676
35-39	19.647444842561175	30.856015223347395	29.64028230805573	19.856257626035706
40-44	19.229164599223452	30.594787966633348	30.25154674476899	19.92450068937421
45-49	19.555681078011993	30.272194767369648	30.169530476797217	20.00259367782114
50-54	19.289367131694778	29.91013329175291	30.64807114736489	20.15242842918742
55-59	19.515339835703713	29.93919042597273	30.685798348731996	19.85967138959156
60-64	19.11914110346604	29.483720143957935	30.996728193979127	20.400410558596896
65-69	19.54301989511723	29.776847071651208	30.306738151011313	20.373394882220243
70-74	19.395278141979162	29.594842390604718	30.30482605279312	20.705053414623
75-79	19.581251214383702	30.152125512078392	29.714603595874493	20.552019677663413
80-84	19.552077634172544	30.151437617752592	29.374787763623523	20.92169698445134
85-89	19.595990746701652	30.231859603302013	29.23780997123369	20.934339678762644
90-94	19.465328151386924	29.740782565924217	29.8079612899792	20.98592799270966
95-99	19.627286454446498	29.808221578137896	29.310889047313882	21.253602920101724
100-101	19.501921343336033	28.927135377673203	30.44511120618089	21.12583207280987
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	66.22713868813885
2	36.21146984441496
3	38.896316215679384
4	36.832750182075614
5	21.450607852424532
6	35.908939275873834
7	38.741679271901276
8	36.568676883272985
9	60.697578776350035
10-14	40.770841927811475
15-19	39.44665703480014
20-24	39.43544974952975
25-29	39.4569537385274
30-34	39.63935262045794
35-39	39.50370246859688
40-44	39.15366528859766
45-49	39.55827475583313
50-54	39.4417955608822
55-59	39.375011225295275
60-64	39.51955166206294
65-69	39.91641477733748
70-74	40.10033155660216
75-79	40.133270892047115
80-84	40.473774618623885
85-89	40.53033042546429
90-94	40.45125614409658
95-99	40.880889374548225
100-101	40.62775341614591
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.5
5	1.0
6	4.0
7	8.5
8	16.5
9	22.5
10	31.5
11	51.5
12	79.5
13	129.5
14	203.5
15	310.0
16	495.0
17	740.0
18	1047.0
19	1510.0
20	2143.5
21	3033.0
22	4349.0
23	5997.5
24	7900.0
25	10238.5
26	13235.0
27	16608.0
28	20628.0
29	25317.0
30	30332.5
31	35755.0
32	41389.0
33	47904.0
34	55670.0
35	65435.0
36	77538.0
37	89263.0
38	97440.0
39	99073.0
40	93978.0
41	85886.5
42	76321.5
43	67959.0
44	62127.5
45	56513.5
46	51498.0
47	46604.5
48	41114.0
49	34903.5
50	29385.5
51	26033.0
52	22974.5
53	19993.5
54	17454.0
55	15000.5
56	13064.0
57	11265.5
58	9362.5
59	7661.5
60	6217.5
61	5048.5
62	3979.0
63	2786.5
64	1885.0
65	1466.0
66	1123.0
67	788.5
68	606.5
69	656.5
70	699.0
71	563.0
72	506.5
73	442.5
74	318.0
75	243.5
76	161.5
77	102.5
78	65.0
79	42.5
80	22.0
81	14.5
82	12.0
83	8.5
84	6.5
85	3.5
86	2.5
87	2.0
88	0.5
89	0.0
90	1.0
91	1.5
92	0.5
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.011968606599540608
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0011331978588926747
20-24	6.748256912506939E-4
25-29	0.017354479569333885
30-34	0.007104768598450701
35-39	0.042106576622000846
40-44	0.05101936877059492
45-49	0.05122308973399135
50-54	0.05133768277590185
55-59	0.042106576622000846
60-64	0.0520379735875771
65-69	0.04087151828140995
70-74	0.004061686707716441
75-79	0.001298721141652279
80-84	2.4191864403326764E-4
85-89	0.0031576749326447566
90-94	0.0025847097230922806
95-99	0.0023045933984221813
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1570776.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	29.345955993602214
#Duplication Level	Relative count
1	100.0
2	7.646043904946037
3	2.967989036000098
4	2.0129218570274836
5	1.5020435133746115
6	1.1318861506081594
7	0.9409950808839725
8	0.7513276718631458
9	0.6344680746922493
10++	4.778395046621473
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATATGGACTTTGGCTACACCATGAAAGCTTTGAGAAGCAAGAAGAAGGT	1935	0.12318752005378235	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CAAAG	409465	2.507775	5.2380524	4
CTTTG	397925	2.4030206	10.859569	9
CCGAG	175015	2.4011688	5.062555	85-89
ACACA	380245	2.2364714	8.38391	6
GCTTT	330150	1.9937353	5.7188005	1
TACAC	338930	1.9841354	10.779924	5
CACAT	316580	1.8532959	6.161984	7
GCCAA	203270	1.8259836	5.344593	1
TGGCT	195720	1.8136514	5.8855815	6
TTGGC	195035	1.8073038	5.2655735	5
GAGAG	178285	1.7365305	5.5630426	7
GACTC	189805	1.6970427	6.185981	7
TTGAG	265220	1.6756103	6.101766	9
GAGTC	179560	1.6717322	9.4702635	9
CACCA	190770	1.6457418	5.102828	7
GGCTT	176295	1.6336483	5.140331	1
ATACA	400705	1.5993159	6.0337186	6
ATGGA	239585	1.5207748	5.354933	4
GTCTT	250445	1.5124068	7.062851	1
AAGAC	246595	1.5102751	6.3634615	5
CATGA	246365	1.5018007	5.804626	9
CCATG	166680	1.4902824	5.925883	9
TGTGT	236450	1.4868515	5.746972	8
GACTT	239100	1.4506891	8.12784	7
TGAGT	227860	1.4395769	7.2228737	8
TATGA	347440	1.4372183	6.909261	4
TCCAT	241815	1.4089838	5.040389	2
TCATA	350045	1.3905762	6.9865847	2
GTGTT	221085	1.3902328	6.320829	1
ATGAG	215240	1.366244	6.1489396	7
ACTCA	231275	1.3539106	5.6908236	8
GTGTA	212225	1.3407978	11.708571	1
GGAGT	136705	1.325298	5.1092615	8
GATTG	207560	1.3113252	6.757277	7
AACAC	221415	1.3022875	5.4580965	5
TATAC	327340	1.3003792	6.446531	5
GTTCT	206840	1.2490815	5.3674154	1
GTATG	194420	1.2283092	6.322312	3
ACACC	141000	1.216384	5.479266	6
TGGAC	129930	1.209669	8.590043	5
GTCCA	133505	1.1936656	8.627495	1
ATGTG	187650	1.1855376	5.666801	7
ACATG	193435	1.1791482	6.4903054	8
GGACT	122760	1.1429151	8.743518	6
ACACT	194655	1.1395328	5.892344	6
AGACT	176995	1.0789325	6.088355	6
TGTAT	259815	1.0697165	5.4861493	2
ACTTT	266505	1.0537504	5.080164	8
GAGTA	165250	1.0489306	5.5986648	1
CATAC	169275	0.9909554	6.0598044	3
GTATA	196995	0.81488836	7.618059	1
ATACT	201100	0.7988827	5.3101554	6
>>END_MODULE