##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-104_TAAGGCGA-CTAAGCCT_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	734874	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.69181519553012	33.0	31.0	34.0	30.0	34.0
2	31.89198420409485	33.0	31.0	34.0	30.0	34.0
3	32.03792078642053	34.0	31.0	34.0	30.0	34.0
4	35.57819435712789	37.0	35.0	37.0	33.0	37.0
5	35.44332089582704	37.0	35.0	37.0	33.0	37.0
6	35.5807948029186	37.0	35.0	37.0	33.0	37.0
7	35.60617602473349	37.0	35.0	37.0	33.0	37.0
8	35.659476318389274	37.0	35.0	37.0	33.0	37.0
9	37.46698073411224	39.0	37.0	39.0	35.0	39.0
10-14	37.596237994540566	39.2	37.2	39.4	34.4	39.4
15-19	38.64518543314908	40.0	38.0	41.0	34.4	41.0
20-24	38.54587834104895	40.0	38.0	41.0	34.0	41.0
25-29	38.367094222955224	40.0	38.0	41.0	34.0	41.0
30-34	38.10413104831577	40.0	38.0	41.0	33.2	41.0
35-39	37.795369546344	40.0	37.8	41.0	32.6	41.0
40-44	37.76035674142778	40.0	37.8	41.0	32.8	41.0
45-49	37.605007933332786	40.0	37.0	41.0	32.2	41.0
50-54	36.926657086793114	39.2	36.4	40.2	31.4	40.6
55-59	37.56281457773714	40.0	36.6	41.0	32.0	41.0
60-64	37.120917327324136	39.0	35.6	41.0	31.6	41.0
65-69	36.449923660382595	38.2	35.0	40.2	31.0	41.0
70-74	35.6031303325468	36.6	34.8	39.2	30.0	41.0
75-79	34.62927549484673	35.4	34.0	38.0	29.0	39.4
80-84	33.69106540713101	35.0	33.8	36.4	28.6	38.2
85-89	32.903418000909	35.0	33.0	35.4	26.6	36.6
90-94	32.302797486371816	35.0	32.6	35.0	25.4	36.0
95-99	31.78955439980187	35.0	32.0	35.0	24.6	35.2
100-101	30.906324077324822	34.0	30.5	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	20.0
7	84.0
8	176.0
9	217.0
10	290.0
11	331.0
12	469.0
13	645.0
14	766.0
15	931.0
16	1034.0
17	1211.0
18	1344.0
19	1577.0
20	1890.0
21	2158.0
22	2539.0
23	2993.0
24	3489.0
25	4116.0
26	4884.0
27	5926.0
28	7127.0
29	8577.0
30	11093.0
31	13835.0
32	18311.0
33	25226.0
34	36382.0
35	56470.0
36	97593.0
37	176873.0
38	208652.0
39	37627.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.95945936748337	23.452501357843023	10.450558859602435	24.13748041507117
2	15.3366800790587	23.618786786525174	39.60653051001574	21.43800262440039
3	16.021947537707867	27.16259608173021	32.74544313635813	24.07001324420379
4	11.564810113832337	18.56448480080362	43.77284167055727	26.097863414806778
5	11.073299747767958	38.793642064627484	38.46368498354979	11.66937320405477
6	25.73853500021092	43.03390953737207	19.542583896133586	11.684971566283423
7	24.346177764532822	33.660329500007485	26.10458162044767	15.888911115012021
8	21.485054874771215	37.92095039157385	24.249409747501208	16.34458498615373
9	22.768621975829774	15.305407343601802	25.68831808575927	36.237652594809155
10-14	20.536519619984094	25.99648572926289	32.65416712708912	20.8128275236639
15-19	19.8271215490188	31.673757189430745	28.645866227043363	19.85325503450709
20-24	19.78271006476723	29.971986736777406	30.26178286958058	19.983520328874786
25-29	19.781286588542162	30.61250206161381	29.655641092494744	19.95057025734928
30-34	19.612749481944114	30.204277587598664	29.870419349026783	20.31255358143044
35-39	19.83717758513652	30.514015753689183	29.702353200857768	19.946453460316526
40-44	19.501207975359264	30.2247792273966	30.1907011483115	20.083311648932636
45-49	19.706177021580967	30.03260375213963	30.157586162879408	20.103633063399997
50-54	19.39267687145741	29.61318069512403	30.568675491029122	20.425466942389438
55-59	19.632997509970053	29.625668129004723	30.597620795301793	20.143713565723427
60-64	19.296521803033727	29.167671467026672	30.90668829595839	20.629118433981215
65-69	19.694506345783193	29.293355729414618	30.314108578532046	20.698029346270147
70-74	19.753827426046286	29.04616429887935	30.176719112672483	21.023289162401873
75-79	20.0956901335846	29.32416223503062	29.79621686751579	20.783930763868984
80-84	20.170467458624966	29.115967136769992	29.678784653845998	21.034780750759047
85-89	20.503444390311614	29.064448611765094	29.51383880090036	20.918268197022936
90-94	20.581692623795238	28.59775981465791	29.89645545771979	20.924092103827064
95-99	21.080377979056447	28.4044979050458	29.38036496894721	21.134759146950543
100-101	21.368232611655486	27.67859876693995	29.9887662391124	20.964402382292164
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	66.09693978255454
2	36.774682703459085
3	40.09196078191166
4	37.66267352863912
5	22.742672951822726
6	37.423506566494346
7	40.23508887954485
8	37.82963986092495
9	59.00627457063893
10-14	41.349347143648
15-19	39.680376583525884
20-24	39.76623039364202
25-29	39.73185684589144
30-34	39.92530306337455
35-39	39.78363104545305
40-44	39.5845196242919
45-49	39.80981008498096
50-54	39.81814381384685
55-59	39.776711075693484
60-64	39.92564023701494
65-69	40.392535692053336
70-74	40.77711658844816
75-79	40.87962089745359
80-84	41.20524820938401
85-89	41.421712587334554
90-94	41.505784727622306
95-99	42.215137126006994
100-101	42.33263499394765
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	1.0
2	2.0
3	3.5
4	5.5
5	6.0
6	7.5
7	12.5
8	14.0
9	17.0
10	21.5
11	25.0
12	42.5
13	60.5
14	79.0
15	118.0
16	165.0
17	228.0
18	320.5
19	455.0
20	674.5
21	1003.5
22	1454.0
23	2081.0
24	2942.5
25	4023.5
26	5297.0
27	6882.5
28	8800.5
29	10881.5
30	13053.0
31	15552.0
32	18331.5
33	21353.5
34	24930.0
35	29331.0
36	34614.5
37	39762.0
38	43212.5
39	43827.0
40	42213.5
41	39333.5
42	36009.0
43	33265.5
44	31128.0
45	28776.5
46	26411.0
47	24152.0
48	21492.0
49	18558.5
50	15918.0
51	14093.0
52	12334.0
53	10563.0
54	9181.5
55	7706.5
56	6438.0
57	5596.5
58	4639.0
59	3684.5
60	2979.5
61	2474.0
62	1972.0
63	1388.0
64	967.5
65	815.0
66	605.0
67	398.5
68	311.0
69	325.0
70	344.5
71	275.0
72	227.5
73	190.5
74	147.0
75	112.0
76	77.5
77	48.0
78	25.5
79	16.5
80	13.0
81	10.0
82	8.0
83	5.5
84	5.5
85	7.0
86	5.5
87	3.5
88	2.5
89	3.5
90	2.5
91	1.5
92	3.0
93	4.5
94	4.0
95	3.0
96	2.0
97	2.0
98	2.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.034427670593870516
2	0.031297882358064105
3	0.028984560618555016
4	0.026807316628428817
5	0.03170611560621277
6	9.525442456802119E-4
7	4.0823324814866224E-4
8	0.002585477238274861
9	4.0823324814866224E-4
10-14	0.005660834374328115
15-19	0.02509273698620444
20-24	0.020493309057062845
25-29	0.0017417951921009588
30-34	0.0015240707930883391
35-39	0.004980445627413679
40-44	0.012355859643966178
45-49	0.006885534118774103
50-54	0.004027901381733467
55-59	0.0031842193355595652
60-64	0.009253286958036344
65-69	0.013961577086684248
70-74	0.01869708276520873
75-79	0.005960205422970469
80-84	0.009661520206185006
85-89	0.007620353965441695
90-94	0.008137449413096666
95-99	0.008273527162479555
100-101	0.004830760103092503
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	734874.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	19.50889221812816
#Duplication Level	Relative count
1	100.0
2	5.205969481862389
3	1.940081605276396
4	1.1978089542227937
5	0.7763680062601308
6	0.531552177072271
7	0.3728131462746632
8	0.28953104913084793
9	0.22022245821921638
10++	1.2537029791515286
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GTCTC	172770	3.1856337	5.4636655	90-94
TCTCT	249725	3.0734391	4.460988	90-94
GCTGC	108870	3.0074654	7.014673	90-94
CTGTC	158695	2.9261107	5.011192	90-94
AGAAG	205150	2.8171637	5.1489515	5
ACACA	208335	2.7257824	8.3875475	6
GACGC	94595	2.6669571	6.800858	85-89
CGCTG	93890	2.5936522	6.594007	90-94
GAAGG	127210	2.5643141	5.6608276	95-97
GCCGA	90525	2.55221	6.907591	90-94
CTGCC	94230	2.540831	6.5802536	90-94
CGACG	89375	2.5197875	6.9418936	95-97
TACAC	187705	2.4062974	10.210461	5
CAAAG	177995	2.3858466	5.1559253	4
CCGAC	86195	2.3720517	6.5716662	90-94
TGCCG	84060	2.3221045	6.5832887	90-94
CACAT	179590	2.302267	5.7442703	7
GGCTT	121060	2.2868302	5.182571	95-97
CTTTG	177240	2.234756	8.939394	9
GACGA	102410	2.0150533	5.2710466	95-97
ATACA	216125	1.9336367	5.0167956	4
CGAAG	96480	1.8983727	5.118355	95-97
GCTTT	149045	1.8792554	5.608749	1
GAGAG	86625	1.7461971	5.7186728	7
GCCAA	86980	1.6705436	5.4782777	1
TGGCT	86435	1.6327623	5.4778366	6
TATAC	184475	1.617156	5.4300523	5
TTGAG	118715	1.5650754	5.7731085	9
GACTC	80650	1.5177048	5.1834707	7
GTGTA	112415	1.4820195	10.47543	1
GAGTC	76670	1.4781353	8.031086	9
AAGAC	108520	1.4546032	5.962149	5
CATGA	108085	1.4195304	5.6238317	9
CCATG	73675	1.3864465	5.776689	9
GTCTT	109115	1.3757921	6.8381476	1
TGAGT	102010	1.3448455	5.5110226	8
TATGA	148890	1.3371671	6.5083294	4
GACTT	103320	1.3295609	6.234171	7
TCATA	150595	1.320155	6.905705	2
AACAC	100465	1.314449	5.1850114	5
GTGTT	101170	1.3068507	6.247376	1
ATGAG	94755	1.2749326	5.3961	7
GATTG	94220	1.2421463	6.4507494	7
GTTCT	96695	1.2191929	5.394675	1
ACACC	62110	1.1643784	5.0818167	6
ACATG	88495	1.1622459	5.929584	8
ACACT	88350	1.132609	5.6073103	6
TGGAC	58155	1.1211811	7.1259446	5
GTCCA	59280	1.1155553	8.0332985	1
GGACT	54400	1.0487878	6.544272	6
AGACT	78775	1.0345886	5.1650524	6
GAGTA	75700	1.0185468	5.2085433	1
GTATG	76565	1.0093921	6.043064	3
CATAC	75410	0.9667238	5.714586	3
GTATA	85380	0.7667898	6.7745247	1
>>END_MODULE