##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-104_TAAGGCGA-CTAAGCCT_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	734874	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.363618797235986	33.0	31.0	34.0	31.0	34.0
2	32.557379904582284	34.0	31.0	34.0	31.0	34.0
3	32.663596752640586	34.0	31.0	34.0	31.0	34.0
4	36.200777276104475	37.0	35.0	37.0	35.0	37.0
5	35.91211826789355	37.0	35.0	37.0	35.0	37.0
6	36.01379420145494	37.0	35.0	37.0	35.0	37.0
7	36.009381200042455	37.0	35.0	37.0	35.0	37.0
8	36.035958545274426	37.0	35.0	37.0	35.0	37.0
9	37.88571510218078	39.0	38.0	39.0	35.0	39.0
10-14	38.06015126402621	39.2	38.0	39.4	35.2	39.4
15-19	39.16813467342701	40.0	39.0	41.0	36.0	41.0
20-24	39.099666881669506	40.0	39.0	41.0	36.0	41.0
25-29	38.896493004242906	40.0	38.0	41.0	35.4	41.0
30-34	38.690810669584174	40.0	38.0	41.0	34.8	41.0
35-39	38.4577728971225	40.0	38.0	41.0	34.0	41.0
40-44	38.221354136899656	40.0	38.0	41.0	33.6	41.0
45-49	38.04291647275587	40.0	37.8	41.0	33.0	41.0
50-54	38.004386602329106	40.0	37.2	41.0	33.0	41.0
55-59	37.621325016261295	39.6	36.4	41.0	32.8	41.0
60-64	37.07509232875295	38.8	35.4	40.4	31.8	41.0
65-69	36.34167163350452	37.6	35.0	40.0	31.0	41.0
70-74	35.767112185218146	36.6	34.8	39.2	31.0	40.8
75-79	34.58822655312339	35.2	33.6	37.4	29.8	39.2
80-84	34.39529252633785	35.0	34.0	36.4	30.8	38.2
85-89	33.757050052117776	35.0	34.0	35.6	30.0	36.8
90-94	33.32840813527217	35.0	34.0	35.0	29.2	36.0
95-99	33.03657524963463	35.0	33.4	35.0	29.0	35.4
100-101	32.66704496280995	35.0	33.0	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	2.0
10	0.0
11	0.0
12	0.0
13	1.0
14	11.0
15	26.0
16	54.0
17	140.0
18	247.0
19	379.0
20	567.0
21	870.0
22	1165.0
23	1678.0
24	2341.0
25	2968.0
26	3814.0
27	4837.0
28	6234.0
29	8113.0
30	10377.0
31	13184.0
32	17005.0
33	22800.0
34	32959.0
35	53271.0
36	97787.0
37	184875.0
38	227862.0
39	41285.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.240675272223534	23.52825110154938	10.946910626855761	24.28416299937132
2	15.578558285882263	23.44051105619005	39.37359143812127	21.60733921980642
3	16.16209037195492	26.771664258090507	33.03069641870579	24.035548951248785
4	11.737386273020952	18.54345098615545	43.40403933191268	26.315123408910917
5	10.762525276441949	38.908574803299615	38.58838385900168	11.740516061256761
6	25.65215261391749	43.302525330873046	19.435712788859043	11.609609266350422
7	24.203332816237886	33.7304082060326	26.055350985338983	16.010907992390532
8	21.335766403492297	38.07863660981338	24.1634620356687	16.42213495102562
9	22.48902532951227	15.248464362598213	25.59146193769272	36.67104837019679
10-14	20.43710894656771	26.00228066307966	32.629076549177135	20.931533841175494
15-19	19.693369780814344	31.797460651308214	28.604087604590557	19.905081963286893
20-24	19.653326438689835	30.02947460094983	30.307265230585003	20.009933729775334
25-29	19.7073872351499	30.680406747476475	29.641190191219874	19.971015826153753
30-34	19.558337899051228	30.236592643519362	29.815566770936503	20.389502686492904
35-39	19.77719131015071	30.58050221123344	29.65162897237204	19.990677506243813
40-44	19.46353516001529	30.239829610371505	30.15108955040185	20.145545679211356
45-49	19.651528741472426	29.979321976485764	30.141309811468926	20.227839470572892
50-54	19.338854182011563	29.638042501258678	30.595536613584795	20.42756670314497
55-59	19.513505330743516	29.588226677622746	30.54048549816682	20.35778249346692
60-64	19.027071212272624	29.271049495180257	30.784024719952274	20.917854572594845
65-69	19.447664746827964	29.39289026207786	30.107316387857153	21.052128603237026
70-74	19.262762110958548	29.254474716643237	30.029625053242086	21.45313811915613
75-79	19.665347237417794	29.62409874382062	29.444582338590365	21.265971680171223
80-84	19.516123457455393	29.54857258406686	29.214419789857683	21.720884168620064
85-89	19.68861221344918	29.55995777110009	28.98531160415659	21.766118411294137
90-94	19.474727736144583	29.05131202397201	29.498609933633347	21.97535030625006
95-99	19.675639338596007	28.959054841663935	29.02875509790287	22.33655072183719
100-101	19.51186156528981	28.070545474833423	30.054492371816373	22.363100588060394
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.52483827159486
2	37.185897505688686
3	40.1976393232037
4	38.052509681931866
5	22.503041337698708
6	37.261761880267905
7	40.21424080862842
8	37.75790135451792
9	59.16007369970907
10-14	41.368642787743205
15-19	39.59845174410123
20-24	39.66326016846517
25-29	39.678403061303655
30-34	39.947840585544135
35-39	39.76786881639452
40-44	39.60908083922664
45-49	39.87936821204532
50-54	39.76642088515653
55-59	39.871287824210434
60-64	39.94492578486747
65-69	40.49979335006499
70-74	40.71590023011468
75-79	40.93131891758902
80-84	41.23700762607545
85-89	41.45473062474331
90-94	41.450078042394644
95-99	42.0121900604332
100-101	41.874962153350204
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	0.5
6	1.0
7	2.5
8	3.5
9	3.5
10	6.0
11	11.5
12	23.5
13	40.0
14	63.5
15	88.5
16	130.5
17	197.5
18	300.5
19	438.5
20	683.5
21	1025.0
22	1502.0
23	2209.5
24	3073.0
25	4102.5
26	5414.5
27	7006.5
28	8792.5
29	10825.0
30	13017.5
31	15296.0
32	18040.0
33	21405.0
34	25153.0
35	29277.0
36	34411.0
37	39641.0
38	43051.5
39	43702.0
40	42126.5
41	39391.0
42	36137.0
43	33327.0
44	31128.5
45	29077.5
46	26946.0
47	24631.0
48	21786.0
49	18619.5
50	15990.0
51	14114.5
52	12277.5
53	10572.0
54	9035.5
55	7454.0
56	6402.5
57	5579.5
58	4548.5
59	3608.0
60	2903.5
61	2405.0
62	1920.0
63	1357.0
64	950.5
65	729.0
66	551.0
67	396.5
68	278.0
69	309.0
70	336.0
71	253.5
72	230.0
73	194.5
74	139.0
75	102.0
76	63.5
77	33.0
78	11.0
79	5.0
80	5.0
81	4.5
82	3.0
83	1.5
84	1.0
85	0.0
86	0.0
87	0.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.011158375449396768
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0011430530948162542
20-24	5.443109975315495E-4
25-29	0.017445167470886164
30-34	0.006776671919267794
35-39	0.04172143796079328
40-44	0.050239905072162035
45-49	0.050947509368953044
50-54	0.05081143161957016
55-59	0.04147649801190408
60-64	0.05092029381907647
65-69	0.0406056004158536
70-74	0.0031297882358064105
75-79	0.001469639693335184
80-84	8.164664962973244E-5
85-89	0.0029664949365469455
90-94	0.002313321739509086
95-99	0.0017690107419775364
100-101	6.80388746914437E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	734874.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Duplicate Percentage	19.948406200228817
#Duplication Level	Relative count
1	100.0
2	5.301769311386474
3	2.012542663550616
4	1.183650823007213
5	0.7885401079408847
6	0.5604407305905365
7	0.3984733594498714
8	0.30992372401656665
9	0.21969276639149027
10++	1.3131126317736268
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	112705	3.171375	7.149321	95-97
GTCTC	171975	3.1605482	5.346733	90-94
CTCCG	116825	3.0787976	7.0706816	95-97
TCTCT	250270	3.0339355	4.4379735	90-94
GAGCC	106900	3.0080292	7.026705	90-94
CGAGC	102670	2.8890026	6.9504685	85-89
AGAAG	204465	2.8539243	5.001236	5
TCTCC	159620	2.7748096	5.220348	95-97
GCCCA	101155	2.692406	6.5846663	90-94
AGCCC	100200	2.6669872	6.4994864	90-94
ACACA	208645	2.6057281	7.762803	6
ATCTC	203215	2.4880621	5.6852417	95-97
CCCAC	98280	2.474384	6.2382536	90-94
CAAAG	181295	2.393636	5.332449	4
CCACG	88170	2.346789	6.453025	90-94
TACAC	188400	2.3296688	10.246245	5
TCCGA	125225	2.32432	5.033901	95-97
CTTTG	176105	2.2569425	8.947892	9
CACAT	180285	2.2293222	5.5398073	7
GAGAC	110525	2.1904051	5.426067	95-97
CGAGA	106800	2.1165824	5.3427863	95-97
ACGAG	99570	1.9732969	5.166616	95-97
GGCGA	65755	1.9560698	6.6206	95-97
AGGCG	65705	1.9545823	6.5580964	95-97
GCTTT	147310	1.8879089	5.8101525	1
TGGAG	86110	1.7863265	5.0391164	5
GAGAG	83695	1.7535357	5.7598023	7
AGACT	132635	1.7338942	6.1735196	6
GCCAA	88265	1.6546315	5.3628035	1
TTGAG	120485	1.6486967	5.9446354	9
TGGCT	84180	1.6355249	5.3129625	6
TATAC	186205	1.6056701	5.6526365	5
GAGTC	77425	1.519278	7.82055	9
GACTC	81450	1.5118057	5.714629	7
GTCTT	114675	1.4696623	6.6241956	1
AAGAC	109365	1.4439452	6.049417	5
CATGA	108520	1.4186467	5.41926	9
TGAGT	101280	1.3858987	5.546557	8
GTGTT	101690	1.3777746	6.661393	1
TATGA	150255	1.369762	6.5593295	4
GACTT	105715	1.3683375	7.2798767	7
CCATG	73705	1.3680496	5.9216156	9
ATGAG	94470	1.3055981	5.280211	7
GTATG	94395	1.2916855	6.030885	3
TCATA	148830	1.2833806	6.568532	2
GATTG	93130	1.2743754	6.1370397	7
AACAC	101730	1.2704868	5.4012637	5
GTGTA	91230	1.2483761	11.325789	1
GTTCT	97220	1.2459607	5.201174	1
ACATG	89065	1.1643177	5.805897	8
ACCAT	93300	1.1537054	5.0361886	8
TGGAC	57870	1.1355586	7.849092	5
ACACC	63355	1.1234208	5.467995	6
ACACT	88900	1.0992969	5.8635626	6
GTCCA	59025	1.095572	7.8568115	1
GGACT	55030	1.0798305	7.5541573	6
GAGTA	74520	1.0298843	5.5082617	1
TGTAT	113545	1.0248877	5.0024376	2
CATAC	76205	0.94231623	5.557793	3
TGCCG	31425	0.87553155	5.1064305	95-97
GTATA	83985	0.76562816	6.4269915	1
>>END_MODULE