##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-103_TAAGGCGA-AAGGAGTA_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1015664	
Filtered Sequences	0	
Sequence length	101	
%GC	39	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.498840167614485	31.0	31.0	34.0	30.0	34.0
2	31.689659178626002	33.0	31.0	34.0	30.0	34.0
3	31.827424226909688	34.0	31.0	34.0	30.0	34.0
4	35.3565056947967	37.0	35.0	37.0	33.0	37.0
5	35.22782140550418	37.0	35.0	37.0	32.0	37.0
6	35.37935872493266	37.0	35.0	37.0	33.0	37.0
7	35.394763425699836	37.0	35.0	37.0	33.0	37.0
8	35.44570054663747	37.0	35.0	37.0	33.0	37.0
9	37.210467241134864	39.0	37.0	39.0	34.0	39.0
10-14	37.31392547141574	39.2	37.2	39.4	33.0	39.4
15-19	38.336398454607036	40.0	38.0	41.0	33.2	41.0
20-24	38.220989224783	40.0	38.0	41.0	33.0	41.0
25-29	38.0333251941587	40.0	38.0	41.0	33.0	41.0
30-34	37.763107878195946	40.0	38.0	41.0	32.0	41.0
35-39	37.43910367995715	40.0	37.0	41.0	31.2	41.0
40-44	37.38710104916587	40.0	37.0	41.0	31.0	41.0
45-49	37.212837513193335	40.0	36.8	41.0	30.8	41.0
50-54	36.55675794357189	38.6	35.4	40.2	30.2	40.6
55-59	37.19561016241592	39.8	36.0	41.0	31.0	41.0
60-64	36.74534373572362	39.0	35.2	41.0	30.6	41.0
65-69	36.07363655697159	37.8	35.0	40.0	29.4	41.0
70-74	35.23720344523386	36.6	34.2	39.2	29.0	40.8
75-79	34.29443812126845	35.4	34.0	37.6	28.2	39.4
80-84	33.41521841868964	35.0	33.2	36.4	27.0	38.0
85-89	32.70368192630634	35.0	33.0	35.4	26.0	36.6
90-94	32.194372351486315	35.0	32.2	35.0	25.2	36.0
95-99	31.79243923187196	34.6	32.0	35.0	24.6	35.2
100-101	31.0126454221081	34.0	30.0	35.0	22.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	3.0
6	34.0
7	114.0
8	227.0
9	333.0
10	433.0
11	566.0
12	925.0
13	1319.0
14	1668.0
15	1912.0
16	2193.0
17	2441.0
18	2718.0
19	3129.0
20	3474.0
21	3925.0
22	4705.0
23	5468.0
24	5789.0
25	6882.0
26	8063.0
27	9401.0
28	11137.0
29	13263.0
30	16171.0
31	20443.0
32	26481.0
33	35664.0
34	50008.0
35	77369.0
36	132741.0
37	240246.0
38	277673.0
39	48699.0
40	47.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.814338843626395	23.289589800967583	10.59250752478056	24.303563830625464
2	14.966104983448911	23.490531752386637	40.38997735736073	21.153385906803724
3	15.667521529140174	27.789825954727743	32.65673135257365	23.885921163558436
4	10.887583638143523	18.57953527033069	44.548280161436146	25.98460093008964
5	10.425093738088837	39.1815854762348	39.06576095039145	11.327559835284918
6	25.164575058632632	43.9902288789598	19.721536834684525	11.123659227723046
7	23.998310456560716	33.79086878568496	26.735449594795103	15.475371162959222
8	20.974956554273927	39.079984443230096	24.16744204364757	15.777616958848405
9	23.033715055939048	15.134114894861368	24.827427315664018	37.004742733535565
10-14	20.421488858111235	25.983226857665787	32.96337789747497	20.631906386748007
15-19	19.749040144634687	31.939444565139674	28.505907052240985	19.805608237984647
20-24	19.613173323175626	30.09447416628769	30.415158881924725	19.877193628611963
25-29	19.647439734836432	30.772904070399417	29.739629445254877	19.840026749509278
30-34	19.429666749635306	30.433434996171886	29.880170705348956	20.25672754884385
35-39	19.723333709463372	30.825432140922338	29.646707552592726	19.804526597021564
40-44	19.317687897563577	30.59590396890528	30.163951924976274	19.922456208554866
45-49	19.65381426610554	30.400400952360325	30.014336606989882	19.93144817454425
50-54	19.42947705805228	29.94306113454736	30.499117973374968	20.12834383402539
55-59	19.705238358082898	29.994208505993434	30.60313260552049	19.69742053040318
60-64	19.336416300628304	29.58738758893629	30.923755837182775	20.152440273252626
65-69	19.736867241519548	29.84304164830986	30.40992486788513	20.01016624228546
70-74	19.623873831289764	29.617291711965514	30.473420212581576	20.285414244163142
75-79	19.725896128126248	30.06255109120273	30.09419785113183	20.11735492953919
80-84	19.59777806837052	29.89853367952382	30.061007408014266	20.44268084409139
85-89	19.689711807730028	29.837392129755447	29.933022966367723	20.539873096146803
90-94	19.549466836661345	29.40202914408309	30.614580391610076	20.433923627645484
95-99	19.82940717496596	29.430157260337207	30.012867721633405	20.727567843063426
100-101	19.81620771108396	28.56180023099577	31.09733823157277	20.5246538263475
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	66.11790267425187
2	36.119490890252635
3	39.55344269269861
4	36.872184568233166
5	21.752653573373752
6	36.28823428635568
7	39.47368161951994
8	36.752573513122336
9	60.03845778947462
10-14	41.05339524485924
15-19	39.554648382619334
20-24	39.490366951787585
25-29	39.48746648434571
30-34	39.68639429847916
35-39	39.52786030648493
40-44	39.24014410611845
45-49	39.58526244064979
50-54	39.55782089207767
55-59	39.40265888848607
60-64	39.48885657388093
65-69	39.74703348380501
70-74	39.909288075452906
75-79	39.843251057665434
80-84	40.040458912461915
85-89	40.229584903876834
90-94	39.98339046430683
95-99	40.556975018029384
100-101	40.34086153743146
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	2.0
1	1.5
2	2.5
3	4.5
4	6.0
5	8.5
6	14.5
7	23.5
8	21.5
9	25.0
10	31.5
11	48.0
12	82.0
13	109.0
14	153.5
15	204.0
16	289.5
17	404.0
18	582.5
19	880.5
20	1236.0
21	1761.0
22	2546.0
23	3619.5
24	4942.0
25	6487.5
26	8467.0
27	10861.5
28	13501.5
29	16403.5
30	19704.0
31	23484.0
32	27358.5
33	31887.5
34	37561.0
35	44403.0
36	52637.5
37	59924.5
38	64561.5
39	64701.0
40	60728.0
41	55472.0
42	49220.0
43	43479.5
44	39107.5
45	35012.0
46	31387.5
47	28129.5
48	24669.5
49	20937.5
50	17704.0
51	15691.5
52	13998.5
53	12327.5
54	10758.5
55	9433.0
56	8421.0
57	7289.5
58	6077.5
59	5157.5
60	4344.0
61	3533.5
62	2870.5
63	2113.5
64	1525.0
65	1267.5
66	1024.5
67	785.5
68	651.5
69	656.0
70	611.0
71	501.0
72	433.0
73	347.0
74	261.5
75	195.5
76	140.5
77	118.0
78	82.5
79	53.5
80	43.0
81	27.5
82	19.0
83	16.5
84	15.0
85	10.5
86	9.0
87	9.0
88	5.5
89	6.0
90	7.5
91	6.5
92	6.0
93	3.0
94	3.0
95	6.5
96	4.5
97	3.0
98	3.5
99	1.5
100	0.5
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03465713070464248
2	0.03180185573181682
3	0.029143496274358447
4	0.02737125663605287
5	0.03298334882402054
6	0.0017722396383055815
7	4.92288788418217E-4
8	0.002855274972825659
9	4.92288788418217E-4
10-14	0.006320988043289907
15-19	0.024673514075521038
20-24	0.020853353077395675
25-29	0.0018903889475259535
30-34	0.0018903889475259535
35-39	0.005690858394114589
40-44	0.013252414184218405
45-49	0.00807353613005876
50-54	0.004765355471888341
55-59	0.003603553931221349
60-64	0.010397139211392743
65-69	0.014394524173348668
70-74	0.018273759826084215
75-79	0.0076993966508609145
80-84	0.01134233368515572
85-89	0.009058113706895193
90-94	0.010121457489878543
95-99	0.009806392665290884
100-101	0.006005923218702248
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1015664.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	29.06474697126442
#Duplication Level	Relative count
1	100.0
2	7.211816603991901
3	3.2181081862886898
4	2.1520345193327546
5	1.5867563397936555
6	1.1100665316748626
7	0.8581621830103172
8	0.6888197859415678
9	0.5080271912062482
10++	3.194605148973098
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CTTTG	262245	2.4215124	10.057102	9
ACACA	226560	2.1252441	8.428917	6
GCTTT	220745	2.0383105	6.020157	1
GCCAA	133135	1.8845551	5.500196	1
TACAC	202840	1.8799819	10.808134	5
TGGCT	127340	1.7958119	6.0453167	6
TTGGC	126340	1.7817093	5.34273	5
GACTC	123860	1.7322961	6.443214	7
CACAT	184570	1.7106498	6.049688	7
GAGTC	117260	1.673676	9.752504	9
CACCA	120120	1.6661012	5.0446987	7
TTGAG	174020	1.6597193	6.2253556	9
GGCTT	117020	1.650274	5.0555625	1
GAGAG	111675	1.6463927	5.633672	7
AAGAC	161155	1.5427651	6.134442	5
ATGGA	158745	1.5323609	5.080506	4
ATACA	244305	1.5313408	5.8025146	6
CATGA	160310	1.516321	5.8665757	9
CCATG	106115	1.484116	5.914134	9
GTGTA	154265	1.4713054	12.117135	1
GACTT	156685	1.464308	7.940119	7
GTCTT	157700	1.456167	6.8040314	1
TATGA	229295	1.4492358	7.0976024	4
TCCAT	157815	1.4451829	5.734783	2
ACTCA	153705	1.4245839	5.726108	8
TCATA	229955	1.4241537	6.969766	2
TGAGT	148990	1.4209952	7.659243	8
ATGAG	138775	1.3395909	6.207152	7
GTGTT	142020	1.3383183	6.2535276	1
AACAC	140800	1.3207731	5.2829194	5
GATTG	136230	1.2992964	6.595427	7
ACACC	90575	1.2563031	5.616211	6
GTTCT	135995	1.2557477	5.442329	1
TGGAC	87780	1.2529019	8.363819	5
GTCCA	87195	1.2195024	9.63404	1
ACATG	125495	1.1870171	6.2565503	8
TATAC	191065	1.1833007	5.978377	5
GGACT	82375	1.1757553	8.720931	6
ACACT	125135	1.1597885	5.788775	6
AGACT	114835	1.0861874	5.9994164	6
TGTAT	171925	1.0736383	5.6707563	2
CCATA	115830	1.0735471	5.0533376	3
GTATG	112460	1.0725895	6.5232873	3
GAGTA	107325	1.0360051	5.235183	1
CATAC	110830	1.0272056	5.9614997	3
GAATA	144630	0.9251844	5.023956	1
GTATA	126965	0.8024694	7.551582	1
>>END_MODULE