##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-103_TAAGGCGA-AAGGAGTA_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1015664	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.442434702815106	34.0	31.0	34.0	31.0	34.0
2	32.62100655334835	34.0	31.0	34.0	31.0	34.0
3	32.727658950204	34.0	31.0	34.0	31.0	34.0
4	36.239905126104695	37.0	35.0	37.0	35.0	37.0
5	35.96717024527796	37.0	35.0	37.0	35.0	37.0
6	36.06711569968021	37.0	36.0	37.0	35.0	37.0
7	36.06443075662818	37.0	36.0	37.0	35.0	37.0
8	36.0871616991446	37.0	36.0	37.0	35.0	37.0
9	37.933872816206936	39.0	38.0	39.0	35.0	39.0
10-14	38.11036957103924	39.2	38.2	39.4	35.2	39.4
15-19	39.24960675971581	40.0	39.0	41.0	36.0	41.0
20-24	39.17398864191308	40.0	39.0	41.0	36.0	41.0
25-29	38.987781786102495	40.0	38.8	41.0	35.8	41.0
30-34	38.790897777217666	40.0	38.0	41.0	35.0	41.0
35-39	38.56758691850849	40.0	38.0	41.0	34.6	41.0
40-44	38.3286096583122	40.0	38.0	41.0	33.8	41.0
45-49	38.158494541501916	40.0	38.0	41.0	33.4	41.0
50-54	38.09846425589565	40.0	37.4	41.0	33.2	41.0
55-59	37.71805892499882	39.6	36.6	41.0	33.0	41.0
60-64	37.16854826005451	39.0	35.6	40.6	31.8	41.0
65-69	36.43542884260937	37.6	35.0	40.0	31.0	41.0
70-74	35.8503164432332	36.6	35.0	39.2	31.0	40.8
75-79	34.691075788843555	35.2	33.6	37.4	30.4	39.2
80-84	34.49262531703398	35.0	34.0	36.6	31.0	38.2
85-89	33.86518986593992	35.0	34.0	35.6	30.2	36.8
90-94	33.4557329983144	35.0	34.0	35.0	30.0	36.0
95-99	33.1878715795775	35.0	34.0	35.0	29.0	35.6
100-101	32.852372438129144	35.0	33.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	1.0
14	11.0
15	37.0
16	83.0
17	176.0
18	296.0
19	453.0
20	695.0
21	1042.0
22	1510.0
23	2158.0
24	2888.0
25	3707.0
26	4955.0
27	6378.0
28	8344.0
29	10609.0
30	13563.0
31	17231.0
32	22473.0
33	30706.0
34	43861.0
35	70628.0
36	129400.0
37	251412.0
38	328207.0
39	64788.0
40	52.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.341723247058084	23.300717560137997	11.024315127837554	24.333244064966365
2	15.224992885425316	23.29641632226217	40.21846914047075	21.260121651841764
3	15.890786716866995	27.281463161045384	33.01534759526773	23.81240252681989
4	11.167078876478834	18.56598245088927	44.279505820822635	25.98743285180926
5	10.264221238519825	39.233545739535906	39.219564737944836	11.282668283999433
6	25.192780289544576	44.09775280013863	19.48991004899258	11.219556861324218
7	23.947092739331115	33.7859764646576	26.748806691976874	15.518124104034406
8	21.029592463649397	39.19514721403929	24.05874383654437	15.716516485766945
9	22.931205595551283	14.99373808661132	24.69615935978828	37.37889695804912
10-14	20.408205863356386	25.87385198254541	32.978957608027855	20.738984546070355
15-19	19.655799224025095	31.967070591346598	28.500900998118055	19.87622918651025
20-24	19.542094705333422	30.08731860806148	30.453287606736033	19.917299079869068
25-29	19.63007152853795	30.799438454299764	29.71093158840307	19.859558428759208
30-34	19.38528622416948	30.412780223666914	29.860210149534023	20.341723402629587
35-39	19.680410112709286	30.81790866473814	29.632365051059207	19.869316171493367
40-44	19.28516530746821	30.54300492369174	30.21299877474135	19.958830994098705
45-49	19.609060028808344	30.349772529938747	30.031091810979284	20.010075630273626
50-54	19.41589611797926	29.922527446979707	30.51325517120721	20.14832126383382
55-59	19.655861715857	29.97936013462306	30.59859549626593	19.766182653254006
60-64	19.23320626599342	29.516637099932936	30.961808081293675	20.28834855277997
65-69	19.688815191590102	29.79921863964654	30.33822371429264	20.17374245447072
70-74	19.486899251596792	29.683811706833897	30.36549763742469	20.463791404144622
75-79	19.648413762085383	30.193933254854734	29.915254137160062	20.24239884589982
80-84	19.62313461638434	30.095472668871075	29.689865451354702	20.59152726338988
85-89	19.649355935567762	30.099833777754498	29.563357929604557	20.687452357073187
90-94	19.434434280682105	29.635683864193652	30.219535691189662	20.71034616393458
95-99	19.670659413714446	29.768926361425134	29.55841903285569	21.00199519200474
100-101	19.489565446840686	28.85767340380283	30.700901085398318	20.951860063958158
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.67496731202445
2	36.48511453726708
3	39.703189243686886
4	37.1545117282881
5	21.54688952251926
6	36.41233715086879
7	39.465216843365525
8	36.746108949416346
9	60.31010255360041
10-14	41.147190409426734
15-19	39.53202841053535
20-24	39.45939378520249
25-29	39.48962995729716
30-34	39.727009626799074
35-39	39.54972628420265
40-44	39.24399630156691
45-49	39.61913565908197
50-54	39.56421738181308
55-59	39.422044369111006
60-64	39.52155481877339
65-69	39.86255764606082
70-74	39.95069065574142
75-79	39.890812607985204
80-84	40.21466187977422
85-89	40.336808292640946
90-94	40.144780444616686
95-99	40.67265460571918
100-101	40.441425510798844
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.5
6	4.5
7	6.0
8	7.0
9	8.0
10	14.0
11	24.5
12	42.5
13	62.5
14	101.0
15	163.0
16	246.5
17	387.0
18	601.0
19	860.0
20	1218.0
21	1767.5
22	2602.5
23	3697.0
24	4974.5
25	6546.0
26	8446.0
27	10699.0
28	13532.0
29	16476.0
30	19587.0
31	23264.0
32	27028.0
33	31394.5
34	36946.5
35	43928.5
36	52271.5
37	59740.5
38	64480.0
39	64940.5
40	60991.0
41	55388.5
42	48797.5
43	42685.5
44	38986.0
45	35478.0
46	31795.0
47	28569.5
48	25119.5
49	21363.5
50	18138.5
51	16033.5
52	14234.0
53	12607.0
54	11102.5
55	9662.5
56	8519.0
57	7450.5
58	6193.5
59	5173.5
60	4336.0
61	3605.0
62	2912.5
63	2108.0
64	1535.0
65	1205.0
66	960.5
67	730.0
68	596.0
69	608.5
70	613.0
71	487.0
72	398.5
73	356.5
74	280.0
75	211.5
76	127.0
77	83.5
78	58.5
79	34.5
80	20.0
81	14.0
82	9.0
83	7.0
84	4.0
85	1.0
86	1.0
87	1.0
88	1.0
89	0.5
90	0.5
91	0.5
92	0.0
93	0.5
94	0.5
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.014079459348761007
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0012405677468139069
20-24	4.1352258227130234E-4
25-29	0.017840545692276187
30-34	0.008112919233132218
35-39	0.044345374060713
40-44	0.05232045243308811
45-49	0.053305030009924544
50-54	0.0534822539737551
55-59	0.0437349359630744
60-64	0.05505757809669339
65-69	0.0453102600860127
70-74	0.003446021518927519
75-79	0.001535941019864837
80-84	1.5753241229382947E-4
85-89	0.003524787725074434
90-94	0.002914349627435845
95-99	0.0022054537721136126
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1015664.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	30.16786794557973
#Duplication Level	Relative count
1	100.0
2	7.556087455728423
3	3.3297085821552894
4	2.230133224793298
5	1.5782855129427917
6	1.1828028844109375
7	0.9373728046461615
8	0.7016627280403867
9	0.5856301903298119
10++	3.398356104465734
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATATGGACTTTGGCTACACCATGAAAGCTTTGAGAAGCAAGAAGAAGGT	1057	0.10406984987161108	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CAAAG	268165	2.5479894	5.1456475	4
CTTTG	264695	2.4637983	10.338937	9
ACACA	227900	2.0966682	8.657695	6
GCTTT	221225	2.0591767	5.922023	1
GCCAA	133965	1.8713688	5.3108125	1
TACAC	203900	1.8630472	11.3185005	5
TGGCT	127755	1.8180202	5.6368203	6
TGGAG	120355	1.7810403	5.0366077	5
GACTC	125485	1.7409294	6.4371347	7
CACAT	187880	1.7166717	6.3968363	7
GAGTC	118540	1.6984954	9.878486	9
TTGAG	171490	1.6599197	6.128904	9
GAGAG	111200	1.6568878	5.9237056	7
GGCTT	115990	1.6505982	5.555121	1
AAGAC	161255	1.5321761	6.1176543	5
CATGA	162045	1.5291582	6.258846	9
ATACA	245270	1.5243285	5.945009	6
ATGGA	155820	1.5186236	5.14572	4
GTCTT	161780	1.5058588	6.6171393	1
CCATG	107470	1.4909964	6.0201	9
GACTT	156010	1.4621454	8.024541	7
TGAGT	150820	1.4598464	7.6587825	8
TATGA	228265	1.4551404	6.938622	4
TCATA	235415	1.4530802	7.207569	2
TCCAT	159370	1.4462206	5.522664	2
TGTGT	149915	1.4411681	5.0616336	8
ACTCA	154800	1.4144174	5.7146244	8
AGACT	148555	1.4018582	6.2222447	6
GTGTT	141570	1.3609457	6.1348276	1
ATGAG	139255	1.3571808	6.350641	7
GTGTA	138450	1.3401124	12.156959	1
GATTG	136185	1.3181884	6.692049	7
AACAC	141245	1.2994466	5.263307	5
GTTCT	135380	1.2601259	5.50676	1
GTCCA	89240	1.2380806	9.526416	1
TGGAC	86295	1.2364744	8.766983	5
ACACC	90975	1.2304953	5.6986117	6
ACCAT	132045	1.2065037	5.1032934	8
ACATG	127445	1.202651	6.601985	8
GTATG	122800	1.1886297	6.6732774	3
TATAC	192335	1.1871724	6.2574997	5
ATGTG	120375	1.1651572	5.0964694	7
GGACT	80975	1.1602468	8.753089	6
ACACT	124990	1.1420417	5.891823	6
TGTAT	172115	1.0896965	5.8978148	2
CCATA	116060	1.0604477	5.0634236	3
GAGTA	108405	1.0565164	5.075842	1
CATAC	113140	1.0337673	6.277227	3
GGATA	92365	0.90019035	5.2837906	1
ATACT	132595	0.8184319	5.4943895	6
GTATA	126545	0.8066972	7.140924	1
>>END_MODULE