##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-102_TAAGGCGA-ACTGCATA_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1697969	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.48454359296312	31.0	31.0	34.0	30.0	34.0
2	31.68962625348284	33.0	31.0	34.0	30.0	34.0
3	31.828530438423787	34.0	31.0	34.0	30.0	34.0
4	35.36099598991501	37.0	35.0	37.0	33.0	37.0
5	35.22294458850544	37.0	35.0	37.0	32.0	37.0
6	35.37480130673764	37.0	35.0	37.0	33.0	37.0
7	35.38482504686481	37.0	35.0	37.0	33.0	37.0
8	35.43711987674687	37.0	35.0	37.0	33.0	37.0
9	37.20901500557431	39.0	37.0	39.0	34.0	39.0
10-14	37.320190533513866	39.2	37.2	39.4	33.2	39.4
15-19	38.34214994502255	40.0	38.0	41.0	33.2	41.0
20-24	38.23568675282058	40.0	38.0	41.0	33.4	41.0
25-29	38.0408197087226	40.0	38.0	41.0	33.0	41.0
30-34	37.76793781276337	40.0	38.0	41.0	32.0	41.0
35-39	37.44595301798796	40.0	37.0	41.0	31.2	41.0
40-44	37.39661042103831	40.0	37.0	41.0	31.0	41.0
45-49	37.22292962945731	40.0	36.8	41.0	30.8	41.0
50-54	36.55941928268419	38.6	35.4	40.2	30.2	40.6
55-59	37.19434512644224	39.8	36.0	41.0	31.0	41.0
60-64	36.7353336839483	39.0	35.0	41.0	30.6	41.0
65-69	36.064036033637834	37.6	35.0	40.0	29.4	41.0
70-74	35.24269807045947	36.4	34.2	39.2	29.0	41.0
75-79	34.31241771787353	35.4	34.0	37.6	28.2	39.4
80-84	33.427766584666735	35.0	33.2	36.4	27.0	38.2
85-89	32.69493518432905	35.0	33.0	35.4	26.0	36.6
90-94	32.15961445703662	35.0	32.2	35.0	25.2	36.0
95-99	31.708832964559424	34.8	32.0	35.0	24.2	35.2
100-101	30.880089683616134	34.0	30.0	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	5.0
6	58.0
7	196.0
8	384.0
9	564.0
10	666.0
11	910.0
12	1438.0
13	2093.0
14	2728.0
15	3128.0
16	3679.0
17	4096.0
18	4694.0
19	5439.0
20	5995.0
21	6728.0
22	7689.0
23	8834.0
24	10063.0
25	11426.0
26	13556.0
27	15641.0
28	18679.0
29	22322.0
30	27513.0
31	34721.0
32	44538.0
33	59831.0
34	84706.0
35	130236.0
36	222539.0
37	397270.0
38	457198.0
39	88343.0
40	63.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.73321401450325	23.42282049212537	10.679604786813817	24.164360706557567
2	15.240724408947834	23.332797231594185	40.003911775327424	21.422566584130564
3	15.926324768601429	27.315046091202145	32.82348671932437	23.935142420872058
4	11.351684428863535	18.155025068938848	43.96327817243872	26.5300123297589
5	10.979341418611693	39.02808167285847	38.56991197156131	11.422664936968527
6	25.96328719103665	43.34415431441468	19.265508240593284	11.427050253955391
7	24.130130580533297	33.82590597165062	26.359281394806462	15.684682053009618
8	21.35827525239735	38.28123619632986	24.179730705487547	16.180757845785248
9	22.630391764234727	15.285577987702892	25.604136728780418	36.479893519281966
10-14	20.473825924890196	26.063288303198455	32.627780595711435	20.83510517619991
15-19	19.731881747755782	31.84170214691119	28.67952461774057	19.746891487592457
20-24	19.665716049849596	30.16975502266507	30.357392574350218	19.80713635313512
25-29	19.63535496798996	30.634625729753566	29.904810322083954	19.82520898017252
30-34	19.548405183228127	30.391592625879156	29.99279125621784	20.067210934674883
35-39	19.702890278676122	30.62891805287754	29.871619736119996	19.796571932326344
40-44	19.45584733634113	30.35192949417888	30.28909398714617	19.903129182333824
45-49	19.56150628127651	30.252527977318945	30.284309542550425	19.90165619885412
50-54	19.385626524469433	29.937890993610992	30.582704678960155	20.093777802959423
55-59	19.522370661588088	29.929564856024705	30.60473911810243	19.943325364284778
60-64	19.319930729562458	29.627353930305723	30.812457511426196	20.240257828705623
65-69	19.587330106934825	29.711775223643794	30.385480876862264	20.315413792559113
70-74	19.522320547117648	29.52647680155016	30.424371484324748	20.526831167007444
75-79	19.778531672409226	29.768907736254675	30.126324469181615	20.32623612215449
80-84	19.818717593958745	29.614199139706663	30.074573284257855	20.492509982076733
85-89	20.01122610770938	29.54678565791117	29.956615157719156	20.4853730766603
90-94	19.991035531571775	29.165868581599636	30.38694462408721	20.456151262741386
95-99	20.341318855738162	29.039596912312316	29.914003546442242	20.705080685507284
100-101	20.44301834851849	28.46798837723967	30.569408652165603	20.519584622076238
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.89757472106082
2	36.6632909930784
3	39.86146718947349
4	37.88169675862243
5	22.40200635558022
6	37.39033744499204
7	39.814812633542914
8	37.5390330981826
9	59.110285283516696
10-14	41.30893110109011
15-19	39.47877323534824
20-24	39.472852402984714
25-29	39.46056394816248
30-34	39.61561611790301
35-39	39.49946221100246
40-44	39.35897651867495
45-49	39.463162480130634
50-54	39.47940432742885
55-59	39.465696025872866
60-64	39.56018855826808
65-69	39.90274389949394
70-74	40.049151714125095
75-79	40.10476779456371
80-84	40.31122757603548
85-89	40.49659918436967
90-94	40.447186794313154
95-99	41.04639954124544
100-101	40.96260297059472
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	3.0
1	3.0
2	3.5
3	10.0
4	14.0
5	14.0
6	21.5
7	38.0
8	51.0
9	48.0
10	62.0
11	99.0
12	142.5
13	198.5
14	268.5
15	366.5
16	514.0
17	740.5
18	1043.0
19	1460.0
20	2159.5
21	3235.5
22	4673.0
23	6463.5
24	8676.5
25	11581.5
26	15407.0
27	19549.5
28	24260.5
29	29508.0
30	34961.0
31	41056.0
32	47214.5
33	53780.0
34	61378.0
35	70113.0
36	80380.0
37	89809.0
38	95611.0
39	96781.5
40	93841.0
41	88772.0
42	82068.0
43	75381.5
44	69560.5
45	63280.0
46	57222.0
47	51528.5
48	45646.0
49	39219.0
50	33523.0
51	29602.0
52	26089.5
53	23050.5
54	20032.0
55	17172.5
56	15252.0
57	13088.0
58	10655.0
59	8782.5
60	7328.5
61	5928.0
62	4616.0
63	3292.5
64	2261.5
65	1790.0
66	1346.5
67	936.0
68	704.5
69	753.5
70	814.0
71	641.0
72	515.5
73	433.0
74	326.5
75	231.0
76	158.0
77	106.5
78	68.0
79	44.0
80	28.5
81	19.5
82	15.0
83	21.5
84	22.0
85	14.5
86	13.5
87	12.5
88	8.5
89	7.0
90	4.5
91	5.5
92	8.5
93	4.5
94	3.0
95	4.0
96	7.5
97	8.0
98	3.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03274500300064371
2	0.031213761853131593
3	0.027385658984351306
4	0.02650225063001739
5	0.03315726023266621
6	0.001943498379534609
7	7.656205737560579E-4
8	0.003003588404735304
9	5.300450126003478E-4
10-14	0.006678567158764383
15-19	0.02382846801090008
20-24	0.019694116912617368
25-29	0.0019317196014768231
30-34	0.0018257105989567537
35-39	0.005371122794350191
40-44	0.011943680950594506
45-49	0.007620869403387223
50-54	0.004899971672038771
55-59	0.0039223330932425735
60-64	0.009399464890112834
65-69	0.013227567758893126
70-74	0.01687898895680663
75-79	0.007008372944382377
80-84	0.010365324690851246
85-89	0.008304038530738782
90-94	0.009128552994783767
95-99	0.009116774216725982
100-101	0.005506578742014725
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1697969.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	26.121848309835993
#Duplication Level	Relative count
1	100.0
2	7.011061227928698
3	2.651565302167712
4	1.7362155916372783
5	1.3444386938362842
6	1.046543817628155
7	0.8437345786743378
8	0.719762165545298
9	0.5506541651119964
10++	4.457590602168915
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
GCTGC	206450	2.5625136	5.6212397	90-94
ACACA	401115	2.2677023	6.95824	6
CGACG	169835	2.1429904	5.520289	95-97
CTGCC	175925	2.141553	5.2059293	90-94
GACGC	169560	2.1395202	5.279567	95-97
CGCTG	170995	2.1224363	5.13364	90-94
GCCGA	168025	2.1201515	5.452968	95-97
CTTTG	374380	2.0542562	7.860464	9
CCGAC	161625	2.0000992	5.2038445	95-97
TACAC	351605	1.955377	8.772927	5
TGCCG	153590	1.9064009	5.0610123	95-97
GAGAG	205360	1.8033122	6.0471134	7
GCTTT	318150	1.7457174	5.1398354	1
TGGAG	191980	1.6583238	5.0952497	5
TTGAG	258765	1.4717674	5.053065	9
GAGTC	165435	1.4014924	6.8279467	9
CCATG	165535	1.3753182	5.668957	9
AAGAC	237475	1.3689419	5.3437524	5
TATAC	360295	1.3452531	5.641861	5
GTGTA	236095	1.3428283	10.398837	1
GTCTT	237980	1.3058175	6.4041657	1
GACTT	223940	1.2491523	6.0973673	7
AACAC	219860	1.2429776	5.0817237	5
TATGA	324180	1.2341897	5.6141996	4
GTGTT	220555	1.2339822	6.1391683	1
TCATA	323050	1.2061894	5.721469	2
GATTG	211180	1.2011201	5.626891	7
GTTCT	215075	1.1801355	5.355437	1
ACACC	138025	1.1433069	5.4228735	6
TGGAC	127125	1.0769469	6.9490514	5
GTCCA	128600	1.0684503	7.210222	1
ACACT	189115	1.0517231	5.1133366	6
AGACT	177705	1.0076855	5.1450524	6
GGACT	117915	0.99892384	6.548507	6
GAGTA	166100	0.9603842	5.003745	1
GTATG	166525	0.94713765	5.018523	3
GTATA	196695	0.74884003	7.062176	1
>>END_MODULE