##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-102_TAAGGCGA-ACTGCATA_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1697969	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.449823288882186	34.0	31.0	34.0	31.0	34.0
2	32.63310460909475	34.0	31.0	34.0	31.0	34.0
3	32.74322911666821	34.0	31.0	34.0	31.0	34.0
4	36.253022287214904	37.0	37.0	37.0	35.0	37.0
5	35.973568422038326	37.0	35.0	37.0	35.0	37.0
6	36.06912493690992	37.0	36.0	37.0	35.0	37.0
7	36.07173099155521	37.0	36.0	37.0	35.0	37.0
8	36.09800591176871	37.0	36.0	37.0	35.0	37.0
9	37.956780129672566	39.0	38.0	39.0	35.0	39.0
10-14	38.14163097206133	39.2	38.2	39.4	35.2	39.4
15-19	39.27881898903926	40.0	39.0	41.0	36.0	41.0
20-24	39.20801993440399	40.0	39.0	41.0	36.0	41.0
25-29	39.01824721181599	40.0	38.8	41.0	36.0	41.0
30-34	38.82221807347484	40.0	38.0	41.0	35.0	41.0
35-39	38.594709208472	40.0	38.0	41.0	34.6	41.0
40-44	38.36071730402617	40.0	38.0	41.0	34.0	41.0
45-49	38.18094664861373	40.0	38.0	41.0	33.4	41.0
50-54	38.12020926177097	40.0	37.4	41.0	33.4	41.0
55-59	37.732966267346455	39.6	36.6	41.0	33.0	41.0
60-64	37.17726660498513	39.0	35.4	40.6	32.0	41.0
65-69	36.43947068527164	37.6	35.0	40.0	31.0	41.0
70-74	35.86753574417436	36.6	35.0	39.2	31.0	40.8
75-79	34.69476109398935	35.2	33.6	37.4	30.4	39.2
80-84	34.500057303755256	35.0	34.0	36.6	31.0	38.4
85-89	33.87209954952063	35.0	34.0	35.6	30.2	36.8
90-94	33.456280061650126	35.0	34.0	35.0	30.0	36.0
95-99	33.174915325309236	35.0	34.0	35.0	29.0	35.6
100-101	32.828663244146384	35.0	33.5	35.0	28.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
11	1.0
12	0.0
13	7.0
14	17.0
15	48.0
16	141.0
17	275.0
18	513.0
19	780.0
20	1260.0
21	1808.0
22	2598.0
23	3632.0
24	4998.0
25	6273.0
26	8381.0
27	10778.0
28	14014.0
29	17475.0
30	22193.0
31	28402.0
32	36938.0
33	50586.0
34	72807.0
35	116617.0
36	215011.0
37	417856.0
38	546927.0
39	117515.0
40	118.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.13113961444525	23.475988077520853	11.071403541525198	24.321468766508694
2	15.417546273608021	23.12849879368191	39.83730020356618	21.616654729143892
3	16.096995881550253	26.89177482038836	33.046127461691	23.96510183637039
4	11.493201583774498	18.04603028677202	43.783367069716824	26.67740105973666
5	10.745897009898297	38.96431560293504	38.895350857406704	11.394436529759966
6	25.972441192978202	43.496907187351475	19.10853496147456	11.422116658195762
7	24.097613089520483	33.790133977711015	26.32121081126923	15.791042121499274
8	21.34473597574514	38.37696683508356	24.077589166822243	16.200708022349055
9	22.571201241012055	15.022064595996747	25.484623099715016	36.92211106327618
10-14	20.42844127307389	25.979944274601007	32.62682652038995	20.964787931935152
15-19	19.62901101800526	31.88455712424644	28.652279103953717	19.83415275379458
20-24	19.553646697669443	30.167659291875392	30.368112142403742	19.910581868051423
25-29	19.598200643091968	30.598951553220015	29.897509131910667	19.905338671777347
30-34	19.499010044677565	30.328136499808227	30.01174894619939	20.161104509314814
35-39	19.625438074389894	30.574431432733775	29.885743906610745	19.914386586265582
40-44	19.375724319920014	30.332497166357946	30.324259629256623	19.96751888446542
45-49	19.539056830440614	30.21111870309531	30.25542928349556	19.99439518296852
50-54	19.299837382239417	29.925132663873132	30.58683457263725	20.188195381250203
55-59	19.45013429192223	29.877557676945944	30.583820183039546	20.08848784809229
60-64	19.14648867395342	29.599239450253677	30.74415342702872	20.510118448764185
65-69	19.381615171520018	29.769325011969332	30.23371047983513	20.615349336675518
70-74	19.31631629355978	29.656911911546977	30.16957633538287	20.857195459510372
75-79	19.557461227914818	29.932794188563093	29.770680899372444	20.73906368414965
80-84	19.46637068886924	29.888739769224955	29.57180747873985	21.07308206316596
85-89	19.598183328694322	29.849475705136864	29.462990746435075	21.08935021973374
90-94	19.40643932620983	29.436245044846395	29.886452273746162	21.270863355197616
95-99	19.584690668850342	29.40769678734349	29.47436602968878	21.533246514117387
100-101	19.44892365462718	28.72851549616954	30.281077146330205	21.541483702873084
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.45260838095395
2	37.034201002751914
3	40.06209771792065
4	38.17060264351116
5	22.140333539658265
6	37.39455785117396
7	39.88865521101975
8	37.54544399809419
9	59.49331230428824
10-14	41.39322920500904
15-19	39.46316377179984
20-24	39.464228565720866
25-29	39.503539314869315
30-34	39.66011455399238
35-39	39.539824660655476
40-44	39.34324320438544
45-49	39.53345201340914
50-54	39.48803276348962
55-59	39.53862214001452
60-64	39.65660712271761
65-69	39.99696450819553
70-74	40.17351175307015
75-79	40.29652491206446
80-84	40.539452752035196
85-89	40.68753354842806
90-94	40.67730268140745
95-99	41.11793718296773
100-101	40.99040735750026
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.0
4	0.0
5	1.5
6	4.5
7	7.5
8	15.5
9	23.0
10	28.0
11	44.5
12	90.0
13	144.5
14	203.5
15	299.0
16	467.0
17	691.0
18	994.0
19	1478.5
20	2228.0
21	3290.5
22	4678.0
23	6482.0
24	8862.0
25	11792.0
26	15225.5
27	19135.5
28	23926.5
29	29141.5
30	34418.0
31	40274.5
32	46543.5
33	53175.5
34	60734.5
35	69575.0
36	79713.0
37	88997.0
38	95152.0
39	96678.5
40	93454.0
41	88042.5
42	81464.0
43	74911.0
44	69422.5
45	63817.0
46	58191.5
47	52775.5
48	46847.0
49	40130.5
50	34355.0
51	30446.5
52	26971.0
53	23708.5
54	20480.0
55	17578.0
56	15599.5
57	13415.5
58	10976.0
59	8971.5
60	7251.0
61	5992.5
62	4751.0
63	3257.0
64	2186.0
65	1708.5
66	1286.5
67	876.5
68	651.0
69	704.5
70	770.5
71	606.5
72	512.0
73	464.0
74	334.0
75	252.5
76	151.0
77	75.5
78	36.0
79	14.0
80	8.0
81	2.5
82	1.5
83	1.5
84	1.5
85	1.0
86	0.5
87	0.0
88	0.0
89	0.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.014193427559631535
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	0.0012367716960674782
20-24	8.009569079294145E-4
25-29	0.017420812747464764
30-34	0.0067492398271110955
35-39	0.041908892329600836
40-44	0.0506369668704199
45-49	0.05049562153372647
50-54	0.05067230320459325
55-59	0.041013705197209135
60-64	0.05187373856648737
65-69	0.04212091033464097
70-74	0.0032509427439488003
75-79	0.0012132141399519073
80-84	2.2379678309792462E-4
85-89	0.002968252070561948
90-94	0.002343976833499316
95-99	0.002037728603996893
100-101	5.889389028892754E-5
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1697969.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	27.089714182168528
#Duplication Level	Relative count
1	100.0
2	7.511108147591366
3	2.685959180209615
4	1.8130679097284945
5	1.4129927440913141
6	1.0723226788063212
7	0.8207602640496093
8	0.6880080499972722
9	0.5698040237862871
10++	4.6608756796731505
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
CCGAG	213305	2.666502	5.7158933	95-97
CTCCG	221585	2.628094	5.620498	95-97
GAGCC	208255	2.603372	5.50505	90-94
CGAGC	193895	2.4238594	5.408782	95-97
GCCCA	198135	2.3726082	5.36972	90-94
AGCCC	195485	2.3408754	5.212949	90-94
ACACA	404385	2.2111335	6.834512	6
CCCAC	187205	2.1473672	5.0435853	90-94
CTTTG	377445	2.0934212	7.9597487	9
TACAC	354745	1.9211972	8.804281	5
GAGAG	203175	1.8306384	6.34313	7
GCTTT	318280	1.7652748	5.085815	1
TTGAG	259510	1.517041	5.1952972	9
AGACT	262645	1.484912	5.5234613	6
GAGTC	166055	1.4195281	6.776641	9
GACTC	170490	1.3960949	5.010489	7
GTCTT	249730	1.3850762	6.333736	1
CCATG	169085	1.3845899	6.1142263	9
AAGAC	237355	1.3548595	5.648636	5
TATAC	364600	1.3502787	5.817769	5
GTGTT	221100	1.28017	6.350934	1
TGAGT	217855	1.2735345	5.1981316	8
GACTT	224500	1.2571399	6.2228103	7
TATGA	323035	1.2489134	5.6741376	4
GATTG	206600	1.2077402	5.6289473	7
TCATA	325035	1.2037518	5.6767197	2
GTTCT	213880	1.1862415	5.279458	1
GTGTA	195630	1.1436119	10.644353	1
ACCAT	207245	1.1223794	5.201694	8
ACACC	138865	1.0997583	5.183736	6
TGGAC	125700	1.0745517	6.7351937	5
GTCCA	130855	1.071535	7.4095836	1
ACACT	187860	1.0173959	5.1202946	6
GGACT	117295	1.0027012	6.5030885	6
GAGTA	165235	0.9752357	5.0829587	1
GTATA	197300	0.7627986	6.857923	1
>>END_MODULE