##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-101_TAAGGCGA-GTAAGGAG_L005_R2_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1584621	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.523518872967102	31.0	31.0	34.0	30.0	34.0
2	31.72211525658186	33.0	31.0	34.0	30.0	34.0
3	31.864356208834796	34.0	31.0	34.0	30.0	34.0
4	35.39153400087466	37.0	35.0	37.0	33.0	37.0
5	35.26337906666642	37.0	35.0	37.0	32.0	37.0
6	35.403894054161846	37.0	35.0	37.0	33.0	37.0
7	35.41743546248598	37.0	35.0	37.0	33.0	37.0
8	35.465774465944854	37.0	35.0	37.0	33.0	37.0
9	37.24211404493567	39.0	37.0	39.0	34.0	39.0
10-14	37.358939708611715	39.2	37.2	39.4	33.2	39.4
15-19	38.38853833187873	40.0	38.0	41.0	33.4	41.0
20-24	38.284156022165554	40.0	38.0	41.0	33.6	41.0
25-29	38.10213129827258	40.0	38.0	41.0	33.0	41.0
30-34	37.83977569399876	40.0	38.0	41.0	32.2	41.0
35-39	37.523656950147696	40.0	37.2	41.0	31.4	41.0
40-44	37.48052348164009	40.0	37.0	41.0	31.4	41.0
45-49	37.31581229833506	40.0	37.0	41.0	31.0	41.0
50-54	36.64418595992354	38.8	35.6	40.2	30.6	40.6
55-59	37.29053281510216	40.0	36.2	41.0	31.0	41.0
60-64	36.843604117325214	39.0	35.2	41.0	31.0	41.0
65-69	36.175839396297285	38.0	35.0	40.2	29.8	41.0
70-74	35.33967869919684	36.6	34.4	39.2	29.0	41.0
75-79	34.37988654700398	35.4	34.0	37.6	28.6	39.4
80-84	33.47335179831644	35.0	33.4	36.4	27.4	38.0
85-89	32.73370275920867	35.0	33.0	35.4	26.0	36.6
90-94	32.19868208234019	35.0	32.2	35.0	25.2	36.0
95-99	31.74556124145774	34.8	32.0	35.0	24.4	35.2
100-101	30.91325906951883	34.0	30.0	35.0	21.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
5	1.0
6	44.0
7	182.0
8	326.0
9	496.0
10	606.0
11	796.0
12	1319.0
13	1788.0
14	2375.0
15	2740.0
16	3207.0
17	3575.0
18	4055.0
19	4722.0
20	5347.0
21	6079.0
22	6867.0
23	7910.0
24	9121.0
25	10435.0
26	12191.0
27	14479.0
28	17243.0
29	20457.0
30	25610.0
31	31750.0
32	40911.0
33	54611.0
34	77584.0
35	119894.0
36	204993.0
37	372657.0
38	440013.0
39	80188.0
40	49.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.867752818067956	23.412879782635045	10.593574502040322	24.125792897256677
2	15.147210980205001	23.572316419489585	39.95853763082457	21.321934969480843
3	15.784632943258876	27.3292419492697	32.661465954440956	24.224659153030473
4	11.148207204511627	18.672001939180923	44.251641549476254	25.928149306831195
5	10.66446136995641	39.07434354019564	38.85137289189522	11.409822197952737
6	25.19785862724198	43.56430145065824	19.781823827094815	11.456016095004957
7	24.25992971154471	33.82655575840915	26.310146389055244	15.603368140990892
8	21.18302268417283	38.534379664076084	24.290587125335655	15.992010526415434
9	22.839597327295262	15.27023649953427	25.205476167036473	36.684690006134
10-14	20.37991734550372	26.106130574514637	32.88487467147854	20.6290774085031
15-19	19.682677947657513	31.893933942577533	28.64817472911609	19.77521338064886
20-24	19.58901577935215	30.0541505535864	30.50811084301212	19.84872282404933
25-29	19.633018189932695	30.83902796860868	29.71949603645307	19.80845780500556
30-34	19.38489659147749	30.400721040647138	29.91495764290086	20.299424724974514
35-39	19.695629668458434	30.874736532934342	29.638834758328485	19.790799040278735
40-44	19.26738356520761	30.553518027421994	30.246569726190298	19.932528681180095
45-49	19.572795127612142	30.296387943132252	30.15712673312867	19.973690196126938
50-54	19.326371426833212	29.88664014215295	30.590159911969785	20.196828519044058
55-59	19.5729471338021	29.92126660063793	30.695044802900185	19.810741462659788
60-64	19.204272665147087	29.41692136764263	31.083722253540042	20.29508371367024
65-69	19.65985212981837	29.603750730237145	30.477965141495595	20.25843199844889
70-74	19.48703823462322	29.282300649132853	30.54736682917605	20.683294287067874
75-79	19.60504570694968	29.699892849979747	30.13022217504317	20.564839268027402
80-84	19.50276689421174	29.446292967645515	30.037603001171387	21.013337136971362
85-89	19.587321374385354	29.426479431326424	29.87236501500296	21.11383417928526
90-94	19.44338098997828	28.88238824734499	30.52434932049362	21.149881442183105
95-99	19.818982523952236	28.824604481838612	29.85649800708857	21.49991498712058
100-101	19.83378025899179	27.841743508638846	30.927360748840822	21.397115483528545
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.99354571532463
2	36.469145949685846
3	40.00929209628935
4	37.076356511342816
5	22.074283567909145
6	36.65387472224694
7	39.8632978525356
8	37.17503321058826
9	59.52428733342926
10-14	41.008994754006814
15-19	39.457891328306374
20-24	39.43773860340148
25-29	39.44147599493825
30-34	39.68432131645201
35-39	39.486428708737165
40-44	39.19991224638771
45-49	39.546485323739084
50-54	39.523199945877266
55-59	39.38368859646189
60-64	39.49935637881733
65-69	39.91828412826726
70-74	40.170332521691094
75-79	40.16988497497708
80-84	40.5161040311831
85-89	40.70115555367062
90-94	40.59326243216139
95-99	41.31889751107282
100-101	41.23089574252033
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	1.0
1	2.0
2	4.5
3	8.0
4	12.0
5	15.0
6	19.5
7	30.5
8	39.0
9	42.0
10	51.5
11	76.5
12	109.0
13	156.0
14	221.0
15	305.0
16	423.5
17	600.5
18	849.5
19	1249.0
20	1842.0
21	2625.0
22	3693.5
23	5208.0
24	7162.5
25	9572.5
26	12640.5
27	16084.5
28	20141.5
29	24816.0
30	29821.0
31	35112.5
32	40673.0
33	47461.0
34	55932.5
35	66508.5
36	79651.0
37	92581.0
38	101175.0
39	102204.0
40	96101.0
41	86901.0
42	76687.0
43	67549.5
44	61568.0
45	56551.0
46	51233.0
47	46283.5
48	40841.5
49	34963.0
50	30112.5
51	26895.0
52	23974.5
53	20940.5
54	18001.0
55	15319.0
56	13485.5
57	11699.5
58	9650.0
59	7816.5
60	6368.5
61	5261.0
62	4136.0
63	2896.5
64	2033.0
65	1658.0
66	1231.5
67	882.5
68	674.5
69	690.5
70	730.0
71	564.0
72	443.5
73	364.0
74	284.5
75	196.0
76	124.5
77	92.5
78	62.5
79	36.5
80	21.5
81	19.0
82	19.0
83	13.0
84	9.0
85	11.5
86	13.0
87	14.0
88	13.0
89	7.0
90	5.5
91	7.0
92	6.5
93	5.5
94	5.0
95	5.0
96	7.5
97	9.0
98	4.5
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.03515036087493476
2	0.03369890970774715
3	0.030228048220994168
4	0.028208637901428798
5	0.03508725430244835
6	0.0018931971745925367
7	5.04852579891343E-4
8	0.002271836609511044
9	5.67959152377761E-4
10-14	0.006146580160177103
15-19	0.022907685812569693
20-24	0.01876789465746068
25-29	0.0019058184890898202
30-34	0.0014135872236957607
35-39	0.004897070024946028
40-44	0.011662094595490027
45-49	0.007358226351916325
50-54	0.0046825076784922075
55-59	0.0036854238332068045
60-64	0.009377636671481698
65-69	0.012886362101726534
70-74	0.01648343673345235
75-79	0.007168906634457072
80-84	0.010917437040150295
85-89	0.008443659398682713
90-94	0.010223264742799698
95-99	0.009794140049892056
100-101	0.005805804668750445
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1584621.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	29.064781199562095
#Duplication Level	Relative count
1	100.0
2	7.568382012053778
3	2.9823341385452506
4	1.960569016421443
5	1.4481614327892052
6	1.2047678305638923
7	0.9327038040162995
8	0.7558011858575507
9	0.6118390552180172
10++	4.537247151257839
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATATGGACTTTGGCTACACCATGAAAGCTTTGAGAAGCAAGAAGAAGGT	1810	0.11422289620041637	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AGAAG	467840	3.0130396	4.998458	5
CAAAG	431130	2.675108	5.514004	4
GCTGC	191795	2.546234	6.039468	90-94
ACACA	420225	2.5121207	8.833247	6
CTTTG	422400	2.4736106	10.686895	9
GACTC	272115	2.4009032	6.6620984	7
TACAC	382610	2.24357	11.152925	5
GACGC	162575	2.2003407	5.80739	95-97
GCCGA	160360	2.1703625	5.8817883	95-97
AAAGC	349175	2.166587	5.0866084	5
CGCTG	161430	2.143114	5.514073	90-94
CGACG	156455	2.1175108	5.892729	95-97
CTGCC	164705	2.1066544	5.596598	90-94
GCTTT	351885	2.0606687	6.1217313	1
CACAT	349195	2.0476296	6.466572	7
CCGAC	150915	1.9678597	5.424321	95-97
TGCCG	145015	1.9251913	5.5397844	95-97
GCCAA	212975	1.9156936	6.09992	1
TTGGC	207930	1.8678355	5.860738	5
TGGCT	206310	1.8532828	6.3949447	6
ATACA	441075	1.7841504	5.599916	4
GAGTC	191005	1.7492076	10.41032	9
GGCTT	188845	1.6963948	5.407702	1
TTGAG	272675	1.6896721	6.533209	9
GAGAG	173410	1.6804302	5.488952	7
CACCA	192295	1.6664447	5.2028804	7
GTGTA	258840	1.6039414	12.739067	1
AAGAC	256015	1.588541	6.4771485	5
CATGA	254590	1.5495272	6.5408998	9
ATGGA	239735	1.5144806	5.595535	4
GACTT	250450	1.4952149	8.097955	7
TATGA	362770	1.4939969	7.3614664	4
GTCTT	255100	1.4938874	7.3460765	1
TGTGT	244355	1.4852623	5.2116356	8
CCATG	168280	1.4847548	5.87065	9
TGAGT	239165	1.4820223	7.521905	8
TCATA	365380	1.4497349	7.4907956	2
TATAC	363005	1.4403114	5.7316155	5
ACTCA	241815	1.4179685	5.7273364	8
ATGAG	220965	1.3959047	6.617383	7
TCCAT	241775	1.3906554	5.6583185	2
GTGTT	227755	1.3843623	6.4454246	1
AACAC	229115	1.369658	5.6111016	5
GATTG	212115	1.3144028	7.3294125	7
ACACC	145485	1.2607852	5.673434	6
GTTCT	211620	1.239265	5.1303234	1
TGGAC	134335	1.2302285	8.897872	5
ACCAT	207050	1.2141117	5.221147	8
ACATG	198045	1.2053738	6.650111	8
GTCCA	136410	1.2035619	9.540075	1
ACACT	202295	1.186229	5.9033704	6
ATGTG	191070	1.1839943	5.5684304	7
GGACT	128570	1.1774334	8.908753	6
ATTGA	273855	1.1278179	5.080954	8
AGACT	182215	1.1090267	6.357595	6
GTATG	175940	1.090239	6.952461	3
GAGTA	170550	1.0774175	5.6477723	1
TGTAT	266585	1.0769085	5.945248	2
CCATA	179060	1.0499823	5.0971475	3
CATAC	174540	1.0234776	6.3401456	3
GTATA	198590	0.81785387	7.201834	1
>>END_MODULE