##FastQC	0.10.1
>>Basic Statistics	pass
#Measure	Value	
Filename	Nextera-101_TAAGGCGA-GTAAGGAG_L005_R1_001.fastq.gz	
File type	Conventional base calls	
Encoding	Sanger / Illumina 1.9	
Total Sequences	1584621	
Filtered Sequences	0	
Sequence length	101	
%GC	40	
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.462995252492554	34.0	31.0	34.0	31.0	34.0
2	32.63883098860863	34.0	31.0	34.0	31.0	34.0
3	32.74832846466126	34.0	31.0	34.0	31.0	34.0
4	36.256043558680595	37.0	37.0	37.0	35.0	37.0
5	35.98584961325137	37.0	35.0	37.0	35.0	37.0
6	36.08329941355062	37.0	36.0	37.0	35.0	37.0
7	36.079828552063866	37.0	36.0	37.0	35.0	37.0
8	36.09992673326934	37.0	36.0	37.0	35.0	37.0
9	37.96346129453036	39.0	38.0	39.0	35.0	39.0
10-14	38.146371277422176	39.2	38.2	39.4	35.2	39.4
15-19	39.284782291790904	40.0	39.0	41.0	36.0	41.0
20-24	39.21711841506581	40.0	39.0	41.0	36.0	41.0
25-29	39.037057820134905	40.0	39.0	41.0	36.0	41.0
30-34	38.840897476431266	40.0	38.0	41.0	35.0	41.0
35-39	38.62485856239441	40.0	38.0	41.0	34.8	41.0
40-44	38.398301675921246	40.0	38.0	41.0	34.0	41.0
45-49	38.22385630381019	40.0	38.0	41.0	33.6	41.0
50-54	38.1742611009194	40.0	37.6	41.0	33.6	41.0
55-59	37.80337102688908	39.8	36.8	41.0	33.0	41.0
60-64	37.26402098672175	39.0	35.6	40.8	32.2	41.0
65-69	36.53496665764243	37.8	35.0	40.0	31.0	41.0
70-74	35.95093564959697	36.6	35.0	39.2	31.0	41.0
75-79	34.753326631415334	35.2	34.0	37.4	30.4	39.2
80-84	34.53758065808796	35.0	34.0	36.6	31.0	38.2
85-89	33.90414414550861	35.0	34.0	35.6	30.4	36.8
90-94	33.49218090634921	35.0	34.0	35.0	30.0	36.0
95-99	33.22134718648813	35.0	34.0	35.0	29.2	35.6
100-101	32.87927428703772	35.0	33.5	35.0	28.5	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
10	1.0
11	0.0
12	1.0
13	6.0
14	21.0
15	50.0
16	146.0
17	223.0
18	416.0
19	691.0
20	1087.0
21	1660.0
22	2377.0
23	3305.0
24	4393.0
25	5693.0
26	7582.0
27	9723.0
28	12522.0
29	16191.0
30	20876.0
31	26265.0
32	33941.0
33	46149.0
34	66105.0
35	105709.0
36	195808.0
37	386928.0
38	527095.0
39	109569.0
40	88.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.42189204863497	23.396509323049486	11.007679438805871	24.173919189509668
2	15.391431784874406	23.37192173257747	39.81648735747096	21.420159125077156
3	15.97416669348696	26.96840443235323	32.85435444816142	24.203074425998395
4	11.397173204192043	18.655754278152315	43.93990739741553	26.007165120240106
5	10.452215387780422	39.189244620638	38.982949235179895	11.375590756401689
6	25.20697378111233	43.75443718087795	19.616362524540566	11.422226513469152
7	24.322282741425237	33.77047256094675	26.254290458096918	15.652954239531091
8	21.09242525499788	38.653343607083336	24.2332393676469	16.02099177027188
9	22.638346961197662	15.16816954969043	25.263769696350106	36.9297137927618
10-14	20.37884137595046	26.026841749541376	32.91178142912406	20.6825354453841
15-19	19.642634464869275	31.92970472430013	28.602470063243434	19.82519074758716
20-24	19.537428662338623	30.071314627342797	30.525053517839513	19.866203192479066
25-29	19.589436248034353	30.844050634125814	29.724466726998887	19.84204639084095
30-34	19.373664420347385	30.42102393283005	29.90037348897267	20.304938157849893
35-39	19.66659191602068	30.783957501227327	29.67913790282012	19.870312679931875
40-44	19.28008632397631	30.516712642560524	30.246299882371584	19.956901151091582
45-49	19.584927923634435	30.240630021964996	30.183577090027708	19.990864964372857
50-54	19.336346526661607	29.86612369919656	30.629406517428066	20.168123256713766
55-59	19.5633917470151	29.82907220746241	30.7170265021351	19.890509543387395
60-64	19.11064510311921	29.36801251099283	31.090345047403673	20.430997338484286
65-69	19.515638613548838	29.625531914893617	30.391704021718542	20.467125449839006
70-74	19.31429230645343	29.442829840175634	30.37256169582	20.870316157550935
75-79	19.58860774671406	29.940017654933143	29.817160120449355	20.65421447790344
80-84	19.48883365795391	29.89176200818468	29.518965818412397	21.10043851544901
85-89	19.562545974652537	29.887866023867186	29.362410770677805	21.187177230802476
90-94	19.331876291824308	29.367401144832755	30.001373233854657	21.29934932948828
95-99	19.569904117554476	29.39809774943137	29.383078055330785	21.64892007768337
100-101	19.32266453618878	28.404489149140392	30.647706928028846	21.62513938664198
>>END_MODULE
>>Per base GC content	fail
#Base	%GC
1	65.59581123814465
2	36.81159090995156
3	40.17724111948535
4	37.40433832443215
5	21.82780614418211
6	36.62920029458148
7	39.97523698095633
8	37.11341702526976
9	59.568060753959465
10-14	41.06137682133457
15-19	39.46782521245644
20-24	39.40363185481769
25-29	39.4314826388753
30-34	39.67860257819728
35-39	39.53690459595256
40-44	39.23698747506789
45-49	39.57579288800729
50-54	39.50446978337537
55-59	39.45390129040249
60-64	39.5416424416035
65-69	39.98276406338784
70-74	40.18460846400437
75-79	40.2428222246175
80-84	40.589272173402925
85-89	40.74972320545501
90-94	40.63122562131259
95-99	41.21882419523784
100-101	40.94780392283076
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	0.0
1	0.0
2	0.0
3	0.5
4	0.5
5	1.5
6	4.0
7	6.0
8	8.0
9	11.0
10	20.5
11	34.5
12	69.5
13	111.0
14	166.0
15	237.0
16	351.5
17	552.5
18	827.0
19	1181.5
20	1729.5
21	2570.5
22	3681.5
23	5300.5
24	7227.0
25	9592.5
26	12630.0
27	16009.0
28	20070.0
29	24653.0
30	29546.0
31	34733.0
32	40301.0
33	47056.0
34	55480.5
35	66066.5
36	79206.5
37	92554.5
38	101576.5
39	102383.0
40	96087.5
41	87144.5
42	77074.5
43	68086.5
44	62258.5
45	56837.5
46	51431.0
47	46693.5
48	41464.0
49	35506.0
50	30320.0
51	27034.5
52	23975.5
53	21054.0
54	18239.0
55	15350.0
56	13413.0
57	11614.0
58	9461.5
59	7673.5
60	6390.0
61	5321.0
62	4083.5
63	2805.0
64	1933.5
65	1539.5
66	1164.5
67	785.0
68	560.0
69	600.5
70	669.5
71	548.0
72	432.0
73	355.0
74	288.5
75	224.0
76	133.0
77	72.0
78	28.5
79	5.5
80	3.0
81	4.0
82	2.0
83	0.5
84	1.5
85	2.0
86	0.5
87	0.0
88	0.5
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.013063060504688502
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10-14	0.0
15-19	8.708707003125668E-4
20-24	5.805804668750446E-4
25-29	0.017821296070164413
30-34	0.006499976966101042
35-39	0.043429943185152795
40-44	0.05174738943886267
45-49	0.05229010596224586
50-54	0.052416319107218695
55-59	0.04200373464695975
60-64	0.05438524416879494
65-69	0.0454998387627073
70-74	0.0035718320027312523
75-79	0.0013504806512093428
80-84	1.135918304755522E-4
85-89	0.0032941630837910137
90-94	0.002536884213953999
95-99	0.002196108722527342
100-101	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	1584621.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Duplicate Percentage	30.046228271423534
#Duplication Level	Relative count
1	100.0
2	7.824447406078994
3	3.057307628529139
4	2.0685038354532224
5	1.4905756557734473
6	1.2344693317283184
7	0.9458123238117465
8	0.7535790398162422
9	0.6565411520485435
10++	4.801532952961191
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CATATGGACTTTGGCTACACCATGAAAGCTTTGAGAAGCAAGAAGAAGGT	1793	0.11315008446814727	No Hit
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	Obs/Exp Overall	Obs/Exp Max	Max Obs/Exp Position
AGAAG	477940	3.0551763	4.8348784	5
CCGAG	199925	2.6900706	6.208619	95-97
CAAAG	432990	2.6493661	5.3161864	4
CTCCG	205840	2.6251686	5.9341903	95-97
GAGCC	191380	2.5750942	5.8860292	90-94
CTTTG	424990	2.5248275	10.645916	9
ACACA	419710	2.4581854	9.018699	6
CGAGC	180990	2.4352927	5.8367543	95-97
TTTGG	379505	2.3554251	5.06447	4
GCCCA	181565	2.33846	5.8014493	90-94
AGCCC	180620	2.326289	5.639084	90-94
TACAC	383780	2.225755	11.888527	5
CCCAC	174235	2.1480007	5.455097	90-94
GCTTT	351375	2.0874872	6.1101136	1
CACAT	349495	2.0269167	6.74283	7
CCACG	154195	1.985949	5.438	90-94
GCCAA	215755	1.9153222	6.108281	1
TTGGC	207890	1.8904821	5.6567073	5
TGGCT	205215	1.8661566	6.2827806	6
GGTTG	192425	1.8280969	5.1404366	7
GAGTC	192955	1.7720069	9.795579	9
GACTC	201105	1.7678013	6.5122643	7
ATACA	441155	1.7634753	5.8123627	4
TTGAG	276430	1.732636	6.922675	9
GGCTT	187550	1.7055169	5.9308496	1
AGGCG	120770	1.6976745	5.3548493	95-97
GAGAG	172580	1.6721257	5.406944	7
GGCGA	118610	1.6673111	5.2321672	95-97
CACCA	194550	1.6531544	5.4711456	7
AGACT	266335	1.6136969	6.257076	6
AAGAC	257535	1.5757974	6.3426375	5
GTCTT	262825	1.56142	7.2364955	1
CATGA	256100	1.551684	6.8886585	9
TGTGT	246985	1.5329301	5.449646	8
ATGGA	241765	1.5303332	5.450465	4
GACTT	253100	1.5185026	8.170961	7
TATGA	366010	1.5135597	7.334126	4
CCATG	171935	1.5113841	6.52505	9
TGAGT	240110	1.5049858	7.1323614	8
TCATA	365630	1.44727	7.575835	2
TATAC	365020	1.4448556	5.776601	5
GTGTT	231090	1.4342767	6.5699363	1
ACTCA	244745	1.419413	5.7418094	8
GTGTA	225995	1.4165144	13.129904	1
TCCAT	244560	1.4044621	5.173994	2
ATGAG	221455	1.4017739	6.113359	7
AACAC	227025	1.3296549	5.4208736	5
GATTG	211925	1.3283249	7.3329306	7
GTATG	204010	1.2787145	6.9135585	3
ACACC	146535	1.2451555	6.0767627	6
GTTCT	209525	1.2447692	5.31214	1
TGGAC	135135	1.2410154	8.753685	5
ACCAT	210445	1.2204881	5.4493766	8
GTCCA	137360	1.2074547	8.601761	1
ATGTG	191265	1.1988301	5.40929	7
ACATG	197775	1.1982989	6.982662	8
GGACT	129315	1.1875674	8.718065	6
ACACT	201965	1.171308	5.9245806	6
ATTGA	273970	1.132947	5.2569923	8
TGTAT	267430	1.0950811	6.0818934	2
GAGTA	171560	1.0859468	5.3560367	1
TGTAG	164990	1.0341411	5.2641187	2
CATAC	174360	1.011211	6.433526	3
GTATA	198885	0.82244825	7.242857	1
>>END_MODULE