FastQCFastQC Report
Wed 6 Aug 2014
3L22C_CGATGT_L004_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 3L22C_CGATGT_L004_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 14844391
Filtered Sequences 0
Sequence length 101
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
TGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 255510 1.7212561970376554 No Hit
TGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 231155 1.5571874925687421 No Hit
TGACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 132577 0.8931117484038248 No Hit
GCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 112166 0.7556120018665636 No Hit
TGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 98993 0.6668714129127965 No Hit
TGACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 89274 0.6013988718028244 No Hit
CAGTAGTTTCACGGATTAAACTTGAAGTTACTAAGGAACCATGCATAGCA 76330 0.5142009530737907 No Hit
TGGCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 71618 0.48245832382076165 No Hit
GCTGCGGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 69125 0.465664101679887 No Hit
GACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 58692 0.39538166301332267 No Hit
AGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 57105 0.3846907562593844 No Hit
GGGTGCATAAGGATGTTGTGTTCAGCTTGGAACACAATCATGAAGTTGAA 53365 0.35949605477247265 No Hit
TGACTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 53003 0.35705742323817796 No Hit
ACAGGAGGAGCTGCAATGAAAGCAATAATAAATACAGAAGTTGCAGTTAA 43219 0.2911470063002248 No Hit
TGACTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 38860 0.2617823796206931 No Hit
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 38078 0.2565143965825206 No Hit
GACCAGACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 37611 0.2533684271722565 No Hit
GCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 36125 0.24335791208948887 No Hit
GACCATACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 35293 0.23775310149133097 No Hit
TGACTGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 34117 0.22983091728047317 No Hit
GCTGTAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 34104 0.22974334211487693 No Hit
TCCTCTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGT 32988 0.22222535097600163 No Hit
TGGCCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 32649 0.2199416601193003 No Hit
TGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 29559 0.19912571691219935 No Hit
TTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 27955 0.18832028878786605 No Hit
GGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 27555 0.18562566830798247 No Hit
TAGATAAGTCTAACTTATAAATTAAATTATAAGTTAAAATTAACCGTTTA 27368 0.18436593323363687 No Hit
GCTGCTGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 26610 0.1792596274242574 No Hit
GACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 26143 0.1761136580139933 No Hit
AGCGTATTGGAAAATTAATCTACCAAAGTAACCGTGAGCAGCTACGATGT 24503 0.16506571404647047 No Hit
GCTGCCGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 23113 0.15570190787887492 No Hit
TGATCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 21417 0.1442767170441684 No Hit
ACAGGGGGAGCTGCAATGAAAGCAATAATAAATACAGAAGTTGCAGTTAA 18839 0.1269098880513185 No Hit
GTTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 18277 0.12312394627708202 No Hit
GACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 18175 0.12243681805471171 No Hit
GCAGATGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 17749 0.11956704724363565 No Hit
GCAGAGGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 17656 0.11894054798206272 No Hit
GCAGTAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 17626 0.11873845144607145 No Hit
TGTCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 17585 0.11846225284688337 No Hit
GACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 17154 0.11555879927980879 No Hit
GGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 17048 0.11484472485263962 No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 16792 0.11312016774551412 No Hit
GACCACACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 16545 0.11145623959918599 No Hit
TGGCTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 16361 0.11021671417843952 No Hit
GCTGTGGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 16351 0.11014934866644244 No Hit
TGCCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 15385 0.1036418402075235 No Hit
TGTCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 14926 0.10054976320685706 No Hit
GCAGACGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 14890 0.10030724736366753 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 9456855 5.1558275 9.007928 7
AGCAG 6471445 4.967181 55.12173 20-24
TAGCA 7464100 4.5561643 35.78756 20-24
CACGG 4251060 4.464106 45.579002 45-49
ACTTG 6222820 4.3831954 106.394 8
CCTGC 3597500 4.110358 50.52101 15-19
GCAGA 5205570 3.9955547 36.43359 25-29
GTTTC 4897230 3.980483 39.203987 40-44
CTGCG 3151720 3.8191483 52.88562 15-19
TCACG 4472330 3.7349448 36.29156 45-49
CGGAT 4005700 3.5478797 35.623173 45-49
GAAGT 5425470 3.5123627 28.231712 60-64
GAACC 4760540 3.445283 31.531315 70-74
CTTGA 4787910 3.372481 31.296316 55-59
AGAGA 5874740 3.2958617 22.148783 90-94
AACTT 6719375 3.2618494 73.44618 7
AGTAG 5037115 3.260948 28.098341 9
AGAGC 4232575 3.2487288 52.818092 95-97
ACGGA 4134200 3.1732206 32.96307 45-49
GCGTT 3048965 3.116195 41.552383 15-19
TTCTC 4034390 3.0918827 31.816511 30-34
GCAGC 2907215 3.052913 18.360445 20-24
TGCGT 2984030 3.049828 44.289135 15-19
CTTGT 3745010 3.0439553 122.81279 9
CCATG 3589155 2.9973853 36.41489 75-79
ACCTG 3554990 2.9688532 29.096561 15-19
CATGC 3482285 2.908136 36.090935 75-79
AACCA 5781620 2.883697 20.520142 70-74
ATAGC 4663810 2.846838 25.862371 80-84
GAGCC 2691150 2.8260193 63.20242 95-97
ACCAT 4902925 2.8218703 30.793901 75-79
TTCAC 4245430 2.8195848 23.970387 45-49
TGCAT 3992665 2.812331 28.751574 75-79
CACTG 3232365 2.6994216 34.456455 85-89
TTTCA 4800455 2.6890512 23.51113 40-44
AAGTT 5221195 2.688098 23.166239 60-64
TGAAG 4128210 2.6725373 22.203608 60-64
CTCAG 3188160 2.6625051 36.176346 35-39
GCACT 3141885 2.62386 27.007908 85-89
AGCAC 3625190 2.6236107 28.5791 80-84
GAGAG 3218240 2.619795 42.73412 95-97
TTGAA 5007270 2.5779598 21.258558 55-59
GACTC 3072620 2.566015 36.059456 25-29
GGAAC 3302560 2.5348923 33.25219 70-74
GCTGC 2086825 2.5287445 34.204914 1
GGATT 3363075 2.5123506 25.325397 50-54
TTGTA 4157790 2.4701276 28.996292 9
AGAAG 4369595 2.4514413 14.918685 6
GTAGT 3229150 2.4123037 25.35978 40-44
AGGAG 2952720 2.4036496 18.503275 90-94
TTAGC 3385810 2.384878 31.014902 20-24
GTAGG 2520610 2.3677523 25.68777 7
ATGCA 3866350 2.3600602 26.762434 75-79
GTTAC 3345955 2.3568053 28.371357 60-64
CATTC 3537670 2.349529 29.206755 30-34
AAACT 5560860 2.3393502 31.375502 6
AGTTT 3933020 2.3365924 26.293266 40-44
GAAGA 4164575 2.3364205 8.422102 50-54
CTGAA 3819155 2.3312519 25.33595 85-89
TCAGT 3293435 2.3198113 30.860992 35-39
CAGTA 3780525 2.3076718 26.526426 35-39
TCTCA 3449305 2.2908418 22.721926 35-39
AGGAA 4078375 2.2880604 24.444532 70-74
GCATA 3733820 2.2791626 20.637047 80-84
CAGAC 3143925 2.2753115 31.232885 25-29
AAGGA 4040935 2.2670557 19.180717 70-74
GTTAG 3032035 2.265051 32.660812 20-24
CTCAT 3396490 2.255765 22.677738 30-34
GTTGT 2605460 2.2460003 13.56028 35-39
ACAGG 2900470 2.2262666 13.137877 60-64
CATAG 3596110 2.195103 26.151213 80-84
AAGAG 3912515 2.1950092 9.272629 50-54
ACCAG 3018425 2.1844852 12.190711 55-59
TAACC 3787850 2.1800904 24.928236 10-14
TGTAA 4226410 2.1759393 22.813099 10-14
TCATT 3856500 2.1602798 24.506474 30-34
GGAAG 2646300 2.1542096 10.56696 6
TAGAG 3313395 2.145039 26.367447 90-94
CGTTA 3013570 2.1226816 23.782364 20-24
AACCT 3683550 2.120061 23.615812 10-14
TAAGG 3266765 2.1148517 26.058504 65-69
TGTTG 2440665 2.103941 12.385247 35-39
CTAAG 3419845 2.087509 26.270329 65-69
GATGA 3212155 2.0794978 9.52141 15-19
CTGCA 2489490 2.0790298 16.397417 3
GTAAC 3398390 2.0744126 26.683052 10-14
CAGCA 2856935 2.067612 15.682704 20-24
ATTCT 3658025 2.0491009 24.731121 30-34
TAGTT 3423135 2.0336719 25.78543 40-44
TTTCC 2631960 2.017086 11.632742 40-44
GGCTC 1664240 2.0166702 18.420378 65-69
AGTTA 3900425 2.008108 22.18651 60-64
TGAAT 3860370 1.9874861 20.319218 85-89
GGAGG 1678610 1.9827472 15.727642 90-94
TAGGG 2093280 1.966337 25.623718 8
AGGGA 2413065 1.9643457 22.382477 9
ACTGA 3214870 1.9623902 25.562277 85-89
AGACT 3211870 1.9605589 26.631678 25-29
GCGTC 1605000 1.9448851 7.2064304 95-97
GATCG 2194135 1.9433624 6.8439093 95-97
CCAGA 2679830 1.939438 16.39679 55-59
CCATC 2457845 1.9353733 13.292366 75-79
GAATA 4315940 1.9256123 14.799271 90-94
TTACT 3433930 1.9235704 18.981827 65-69
ACTCA 3339520 1.9220552 23.505367 25-29
CAGAA 3628185 1.9192415 11.169438 6
CAGGC 1810945 1.9017023 14.167782 65-69
GAGCG 1703125 1.8968102 8.573373 90-94
TTAAA 5343175 1.8958585 15.898582 50-54
TTCCG 1949165 1.8783679 14.485202 40-44
CGTCG 1546560 1.8740696 7.4846716 95-97
AATAG 4186230 1.8677404 18.930016 90-94
TGCAG 2090650 1.851705 18.004099 4
GGAGC 1659510 1.8482354 15.784742 95-97
TGACC 2190990 1.8297458 63.93102 1
ATCGG 2037200 1.8043638 6.2789073 95-97
ATTAA 5065560 1.7973557 15.885987 50-54
TTGTT 2595030 1.7790203 11.251877 35-39
AGCTG 2006565 1.7772303 8.944409 9
GATGT 2366085 1.7675595 15.516615 30-34
TCGGA 1991705 1.7640686 5.9883137 95-97
CCAAA 3529755 1.760535 28.09078 4
GGATG 1842180 1.7304643 17.178865 10-14
TAAAC 4095320 1.7228248 17.403254 50-54
TACTA 3546080 1.7214068 21.012758 65-69
AGATC 2816120 1.718989 5.727304 95-97
ATGAT 3329850 1.7143514 8.477835 15-19
GATTA 3314200 1.706294 22.155088 50-54
ACCAA 3415290 1.7034434 17.61772 3
TGATG 2257230 1.6862407 11.89722 30-34
CGGAA 2179405 1.6728104 8.835152 5
TGTTT 2437190 1.6708133 14.555417 35-39
CAGAG 2172225 1.6672995 11.931671 25-29
AGCGT 1874270 1.6600554 6.5118866 90-94
GAGAT 2563435 1.659527 13.582862 25-29
GTCGT 1619335 1.6550415 6.5027485 95-97
CCTCC 1530540 1.6488584 8.008752 1
TCCGT 1707105 1.6451001 14.900401 40-44
TCCTC 1805375 1.6404363 7.825832 1
ATAGA 3588265 1.6009507 18.768093 90-94
CCACC 1710175 1.5966053 6.3462777 1
CAGGA 2058735 1.5801897 10.161418 90-94
AAGAA 4066740 1.5723823 8.944973 45-49
AGGCT 1772275 1.5697176 13.210515 65-69
GAGCT 1771125 1.5686991 9.20088 95-97
ACTAA 3695305 1.5545462 18.436323 65-69
TCTCC 1687100 1.5329669 5.0537148 1
GCTCA 1835100 1.532534 16.977839 65-69
CTACA 2658470 1.5300779 9.48647 85-89
CATCA 2617235 1.5063453 7.579285 80-84
TACCA 2579105 1.4843996 8.871602 75-79
CTCAC 1868260 1.4711182 12.929602 65-69
GGGAT 1563775 1.4689428 19.208002 10-14
TCGTG 1422365 1.4537282 5.6306357 95-97
TACAG 2377700 1.4513727 8.265268 90-94
TAAAG 3232100 1.4420431 7.890569 45-49
GAGGA 1768080 1.4392983 14.118734 95-97
ATACC 2492740 1.4346921 8.5504675 75-79
TGCTG 1402760 1.4336909 6.1172504 1
AACAG 2674175 1.4145881 12.672549 60-64
AAAGA 3639250 1.4070958 6.6853065 45-49
GTAAA 3137960 1.4000413 7.0941553 45-49
GACCA 1931235 1.3976673 23.18234 2
CTGCT 1447660 1.3950783 6.059445 2
GGGGG 813170 1.393694 5.7001886 1
CTCTT 1811285 1.3881358 5.386128 3
ATGTT 2333070 1.3860683 10.080868 30-34
GAAAC 2612400 1.3819103 8.180455 60-64
AGCCA 1901150 1.3758942 11.840207 95-97
GAGAA 2451860 1.3755487 11.984864 50-54
GCTCC 1169700 1.3364519 6.30597 1
TCTAC 1988910 1.3209264 11.81515 85-89
AGAAA 3407035 1.3173112 5.8424573 60-64
CCGTA 1566680 1.3083702 16.98229 40-44
CAAAC 2610605 1.3020908 30.46067 5
CGTGT 1267040 1.2949784 5.1414466 95-97
AAGTA 2876675 1.2834657 20.240881 8
AGATG 1973205 1.2774215 10.892435 25-29
AGAAC 2304950 1.2192751 10.024328 50-54
GCTGG 940500 1.2086982 6.129968 1
GCCAA 1666610 1.2061536 11.926605 3
GATAC 1966780 1.2005429 7.75063 75-79
GGCCA 1134850 1.1917241 14.519464 2
GTGCT 1158815 1.184367 10.306903 9
CAATA 2814975 1.1842077 7.8360744 80-84
AAACA 3220430 1.174047 6.133743 60-64
CCCAA 1705590 1.1638666 11.303763 4
CCGAA 1603830 1.1607187 32.418736 4
GCCCA 1152100 1.1407417 6.685911 2
CCTCT 1255100 1.1404343 6.410139 2
TTGGT 1310595 1.1297799 7.9509974 7
TCAAT 2325170 1.1287291 6.770489 80-84
CTTTT 1728485 1.117285 5.081451 6
CCTTT 1457005 1.1166221 5.1154 4
TGGCC 918795 1.1133649 30.92119 1
CGTAA 1822575 1.1125188 10.1391115 40-44
CCAAC 1628565 1.1113061 8.9829855 5
GATAG 1714540 1.1099659 13.330691 15-19
TGATA 2149035 1.1064166 8.353397 15-19
CTCCC 1024435 1.103629 5.073641 2
ATCAA 2569505 1.080943 6.0821166 80-84
TGCCA 1278445 1.0676585 5.3208675 1
TATCT 1872205 1.0487455 7.467368 85-89
GTTGC 1005445 1.0276152 5.5015607 1
TCAAC 1781870 1.0255522 5.780796 7
ATCTA 2105295 1.021993 6.732719 85-89
GGATA 1574945 1.0195943 10.244835 70-74
CAACT 1767940 1.0175349 7.7663426 6
CCATA 1726490 0.9936784 5.47534 3
AATAT 2716135 0.96373564 5.4125614 80-84
GGGTG 700810 0.95521134 11.171955 1
ACCCA 1395935 0.95256317 7.8996477 3
GGTGC 734815 0.9443589 9.800681 2
CCTAA 1628040 0.93701565 12.770746 4
ACCGA 1292820 0.93563557 21.84011 3
TGGTC 911125 0.9312154 8.857658 8
GTGCA 1048980 0.9290897 7.412136 3
GACCT 1102055 0.9203512 12.997755 2
TGTGC 895915 0.91567004 9.87615 8
ATTGG 1224245 0.9145598 7.662465 6
CTCCG 799615 0.9136078 6.349645 2
GAACT 1486275 0.90723777 28.252094 6
ACCTA 1561660 0.8988108 9.193401 3
CTGCC 782435 0.89397854 5.8686275 2
CGAAC 1233235 0.8925129 30.330673 5
CCGCC 649695 0.8801063 5.7911873 1
CCGTT 903855 0.8710253 6.7590117 4
ATATC 1790940 0.86939275 5.8773313 85-89
TGTCC 897570 0.86496866 7.276222 1
GGCTG 671655 0.8631878 7.1148524 1
CCCAG 871770 0.8631754 5.202448 2
TGCCC 747375 0.8539204 8.238935 1
GCTGT 835135 0.8535498 7.7759485 1
TCCCC 772760 0.83249825 5.8230267 1
GGTGT 750000 0.8129682 7.7885146 6
GCAGG 727310 0.8100222 6.4585085 1
CTGTC 836900 0.8065022 7.315213 4
GGCCT 645965 0.7827588 7.7680793 2
CCGTC 678505 0.7752323 6.383318 4
TGGCT 753185 0.7697928 5.291373 1
TGTCA 1065310 0.75037706 5.672529 5
CCCCC 583385 0.7451449 7.0575576 1
GTGTG 683235 0.74059784 10.484097 7
GTCAA 1211290 0.73938394 5.9052644 6
GGGCC 474430 0.7228996 8.595171 1
CGCCC 525850 0.71234024 5.384477 1
GACCG 644355 0.6766474 30.252148 2
TGCGG 522905 0.6720194 13.839305 3
AGTCT 942830 0.66410524 5.5926957 7
GGTCA 744315 0.6592456 7.469924 9
CAGGG 582605 0.6488608 6.425451 2
TAACT 1310635 0.63623387 10.534874 6
CTAAC 1105430 0.6362283 11.621833 5
GGCCG 413500 0.63005924 16.64243 2
CCCCG 462815 0.62695014 7.821982 2
GACCC 632635 0.6263979 10.789086 2
GCCCC 457600 0.6198857 6.6834536 1
GGTAG 652460 0.61289275 5.5934753 6
TGACT 866795 0.6105482 11.720748 1
GACTG 675475 0.59827346 7.6157384 2
GCCGA 562870 0.59107876 11.816996 3
GGCCC 410605 0.5899157 7.106401 1
GTCCC 515640 0.58914936 5.0025477 1
TCCCG 513910 0.5871728 5.588774 2
GCCGT 482160 0.58426535 5.286683 3
GACTT 823250 0.57987624 8.565433 6
CCCGA 548235 0.54283005 6.8852315 3
GCGGA 478700 0.53313947 10.963825 4
GCCCG 364830 0.5241508 7.7118297 2
GTCTA 738230 0.5199903 5.6280155 8
CCCGT 448425 0.5123523 7.4075727 3
GTCCG 374210 0.45345512 5.5047154 2
Produced by FastQC (version 0.10.1)