FastQCFastQC Report
Wed 6 Aug 2014
3L22C_CGATGT_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 3L22C_CGATGT_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 14844391
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 200278 1.349183001175326 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 125402 0.8447769935459124 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 114952 0.7743800335089529 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 93236 0.6280890876560715 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 79140 0.533130661944973 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 76794 0.5173267128304556 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 68780 0.46333999151598737 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 65252 0.43957343888341394 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 64165 0.4322508077293302 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 62076 0.41817815227313804 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 51996 0.35027371618007097 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 47895 0.3226471197100642 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 42407 0.28567692672606104 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 41468 0.27935130514953427 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 40847 0.275167906854515 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 40604 0.27353092491298564 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 37512 0.25270150860348534 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 37058 0.24964311435881742 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 36788 0.247824245534896 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 36299 0.24453007199823823 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 34498 0.23239754328756226 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 34164 0.23014753518685946 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 32768 0.22074330971206566 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 32190 0.21684958311863384 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 29835 0.20098500504331906 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 29757 0.20045955404974172 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 28054 0.18898720735663727 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 27843 0.18756579505349866 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 26761 0.1802768466554135 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 26275 0.17700288277235487 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 26193 0.17645048557397874 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 24855 0.16743698006876806 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 24656 0.16609640638002596 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 24319 0.163826188625724 No Hit
ATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAA 24014 0.16177154050981277 No Hit
GCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATG 22389 0.15082464481028557 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 22103 0.1488979911671688 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 20947 0.14111053798030515 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 20808 0.1401741573635456 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 20140 0.13567414116213997 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 19785 0.13328266548624326 No Hit
GTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGG 18893 0.1272736618161028 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 18852 0.1269974632169147 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 18843 0.12693683425611735 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 18311 0.12335298901787214 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 17911 0.12065836853798852 No Hit
GCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGA 17714 0.11933126795164585 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 17276 0.11638065852617328 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 17170 0.11566658409900414 No Hit
TTCATGCGTTGGAGAGACCGTTTCTTATTCTGTGCAGAAGCTATTTACAA 16945 0.11415086007906959 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 16919 0.11397570974787716 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 16834 0.1134031028959019 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 16410 0.11054680518722526 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 16202 0.10914560253768579 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 16161 0.10886940393849771 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 16028 0.10797344262893642 No Hit
ATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTC 15940 0.10738062612336201 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 15167 0.10217327204598693 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 15050 0.10138509555562099 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 15023 0.10120320867322884 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGCT 4703625 3.5398393 7.408129 5
AAAAA 5327825 3.4548845 5.4615602 45-49
ACTTC 4925355 3.4224317 6.5119987 60-64
AACTT 5553820 3.1216474 5.6643143 55-59
GGAAG 3776070 3.1108882 6.019438 85-89
AGCAC 2838340 3.046575 6.599484 90-94
AGCTG 3866395 3.0444148 7.5684996 3
CTACT 4191495 2.9125018 8.804571 2
GAAGA 3809865 2.8980129 5.9349065 90-94
CTGGT 4173790 2.7518826 13.515452 1
CTTCA 3912215 2.7184412 6.558417 60-64
TCTCT 4630285 2.6940563 8.055815 20-24
TTCAA 4666205 2.622744 8.234521 9
GCTGC 2673480 2.6024337 7.759674 3
CTTCT 4451280 2.589905 5.194166 20-24
CAACT 3080245 2.5561175 8.677671 9
CGGAA 2706570 2.5451603 6.7569046 95-97
TGGTG 4366685 2.5223165 9.091306 6
GGTGT 4360370 2.5186687 10.24766 1
CTGAA 3461020 2.516217 5.7461233 80-84
CGGTG 2948055 2.5141242 12.2710495 9
GGTGG 3337875 2.4938464 10.726252 7
ACAAC 2515450 2.492927 6.228739 85-89
TATGC 3945660 2.4019575 6.8872757 30-34
ACCCA 1956700 2.3973048 29.587322 1
CAACA 2403130 2.3816128 6.0528154 85-89
AGGAA 3129100 2.380182 5.5794606 40-44
ATGCA 3273445 2.3798468 6.4769983 1
CAAGC 2208760 2.370806 6.595715 75-79
CCGTG 2417355 2.353115 6.1067023 60-64
TCTGC 3090000 2.3254623 5.6545143 85-89
TCAAC 2757550 2.2883315 7.676778 8
GCAGC 1968225 2.2881043 6.2168617 1
ACATC 2755705 2.2868004 5.4806113 40-44
AGAGC 2430280 2.2853475 7.5544014 95-97
CATGG 2885920 2.272385 9.924138 2
CAGCA 2106380 2.2609148 5.3883133 2
CGTGA 2852740 2.2462587 8.152589 9
GAAGC 2385630 2.24336 6.0849495 15-19
ATCGG 2834870 2.2321877 5.749839 95-97
TCGGA 2833200 2.230873 5.5369205 95-97
AAGAG 2920915 2.2218242 5.273858 90-94
ATGGT 4165850 2.221763 5.314802 5
GCTGG 2562555 2.1853669 8.630417 4
ACTGC 2429980 2.183996 7.346687 4
CCAAG 2033275 2.1824462 6.3631387 75-79
TCCTT 3710750 2.1590397 6.190846 40-44
GAGCA 2287890 2.151449 6.2573857 90-94
GATCG 2715500 2.1381955 6.2475853 95-97
CTGCA 2349120 2.1113214 5.2652473 95-97
GCACA 1937520 2.0796664 6.8234496 95-97
CAAGG 2196350 2.0653682 5.8002257 10-14
CAGCT 2296615 2.0641315 5.141023 2
CTTGG 3069565 2.02384 6.3843927 2
GGCTC 2074220 2.0190988 5.6825123 10-14
CTCTG 2667155 2.007239 6.334083 45-49
CCTTA 2879205 2.0006437 6.581105 45-49
GCTAC 2225635 2.0003366 11.091609 1
CTCTC 2327855 1.9996699 5.2937226 15-19
TAGCT 3228565 1.965419 5.518477 2
TGGCT 2953175 1.947101 8.1695385 9
CACAC 1585490 1.9425067 7.8105116 95-97
AGATC 2657735 1.9322159 5.7593946 95-97
TATTC 4089195 1.9245604 6.290964 2
CCAGC 1439165 1.9096913 6.4322715 1
CATCC 1843935 1.891674 6.1323614 90-94
CTCTA 2717920 1.8885735 5.4915137 95-97
GTGGC 2200215 1.8763604 9.822779 8
TGCTG 2842675 1.8742455 5.7298503 6
TTCCA 2696220 1.8734951 5.0500298 25-29
CTCCT 2167695 1.8620895 5.6529627 70-74
AAGGA 2433470 1.8510439 5.1575313 10-14
TTCGG 2798705 1.8452553 9.360116 7
GCATG 2333730 1.8375882 5.6164465 3
CACAA 1842505 1.8260075 6.221526 4
TTTCA 3865955 1.8194937 7.1049843 8
CACCC 1188820 1.8006123 7.236814 50-54
GCAGG 1765515 1.7981294 5.7187743 90-94
GCTTC 2366470 1.7809503 6.986431 15-19
TCGGT 2700610 1.7805786 10.351264 8
GCTCT 2343565 1.7637125 5.724099 10-14
TACTG 2894525 1.7620691 5.180006 3
AATGA 2959690 1.7405441 5.695806 75-79
GCACC 1301400 1.7268851 6.525726 50-54
CCTGT 2280730 1.7164243 7.3898954 1
TGAAC 2355300 1.7123408 5.3617744 80-84
TTGGT 3826410 1.7087847 13.167625 7
ACCAG 1581070 1.6970652 5.1328163 2
CCACA 1383945 1.6955782 6.408785 1
GTGTA 3153540 1.6818702 9.211204 2
TGTAG 3103565 1.655217 5.9270244 3
CATGC 1840170 1.6538918 5.316617 2
CCCAA 1346670 1.6499097 29.520206 2
GTAGC 2069475 1.6295128 6.331323 1
CGCAG 1397950 1.6251471 6.6098123 90-94
CTTTC 2769850 1.6115923 8.346324 7
GCTTG 2434415 1.6050698 6.055168 1
GTCTG 2434250 1.6049609 6.0718355 90-94
TGGTA 2979035 1.5888019 10.015562 2
CCGCC 951150 1.5602952 6.697357 45-49
CCAAT 1869075 1.5510374 20.278952 3
GAACA 1771220 1.537853 5.192449 80-84
TACTT 3243620 1.5265945 7.495981 5
ACACA 1527735 1.5140558 5.575352 3
ACACG 1372180 1.47285 6.675811 95-97
AACAC 1483620 1.4703358 5.5400424 85-89
ATGCC 1616535 1.4528949 6.5577044 9
GCTGT 2202620 1.4522415 5.874643 7
ACGGT 1832045 1.4425595 6.3958936 4
AGCCT 1574015 1.4146793 6.140122 2
ACATG 1945075 1.4141008 9.137302 1
GTATT 3427375 1.4132007 5.872558 4
GGTAC 1747920 1.376319 11.871108 3
GGTCA 1740690 1.3706262 18.799662 9
TGTAT 3242015 1.3367715 5.653486 3
ACTTT 2837220 1.3353244 7.3016467 6
AACGC 1240675 1.3316972 5.6664596 95-97
GACGG 1296905 1.3208628 8.157281 3
TGGTC 1967500 1.2972211 15.955826 8
ATGGG 1860685 1.283568 8.683959 3
GCGGC 1013960 1.2766607 5.572654 7
GCCTG 1307230 1.2724909 5.287941 3
ATTCG 2023095 1.2315781 7.639479 6
GGTCG 1425510 1.2156861 7.990509 6
AAGCC 1126145 1.2087647 6.0767474 1
CAATT 2126705 1.1953616 13.861578 4
TGGGT 2043645 1.1804651 6.2703714 4
CTGAC 1286505 1.1562736 7.198914 1
GTACT 1897910 1.1553705 9.130009 4
TGACG 1431840 1.1274365 6.2744465 2
GGGTC 1282015 1.0933125 7.949526 5
ATTGG 2044405 1.0903378 13.004643 6
TGGCC 1088605 1.0596758 8.332963 1
TGGGG 1365060 1.0198854 5.438282 8
TCGTG 1494725 0.985509 6.2351775 8
CGGTA 1178530 0.9279791 5.861502 5
AATTG 1872200 0.921919 11.204891 5
GGGGT 1233145 0.92132705 5.188734 8
GCCTA 878455 0.78953 5.931985 3
GGCCT 757975 0.73783225 5.0888066 5
GTCGT 1051680 0.6933985 5.9622188 7
Produced by FastQC (version 0.10.1)