FastQCFastQC Report
Thu 31 Jul 2014
3L22B_CGATGT_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 3L22B_CGATGT_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 10858564
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 144486 1.3306179343787998 No Hit
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 142421 1.3116006867943129 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 116414 1.0720938790801435 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 108744 1.0014583880520482 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 86743 0.7988441197196978 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 62493 0.575518088763855 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 61307 0.564595834218963 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 60300 0.5553220481087554 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 57115 0.5259903611564107 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 53487 0.4925789450612438 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 46124 0.4247707155384451 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 45093 0.41527590572749773 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 41773 0.38470096045849156 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 41048 0.3780242028319767 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 40752 0.37529824385618576 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 40385 0.3719184230990396 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 39441 0.36322482420327407 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 38255 0.3523025696583821 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 35772 0.3294358259526766 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 35672 0.32851489386626076 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 35542 0.32731768215392015 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 31534 0.29040672413037305 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 31268 0.2879570447805069 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 31224 0.2875518346624839 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 28093 0.25871745103680377 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 27061 0.24921343190499223 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 26648 0.24540998238809478 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 25441 0.23429433210505551 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 25347 0.23342865594382461 No Hit
CGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTC 24184 0.22271821577880832 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 23698 0.2182424858388273 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 22540 0.20757809227813181 No Hit
GTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTCTGCT 21931 0.2019696158718593 No Hit
ATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTC 21847 0.20119603291926996 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 20643 0.1901080105988232 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 20383 0.187713587174142 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 20289 0.1868479110129111 No Hit
TAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTA 19992 0.18411274271625605 No Hit
ATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGATTTCACTAAA 19746 0.18184724978367306 No Hit
GGTCCTTACGAGTTAATCGTTCTTCACTTCTTACTTGGTGTAGCTTGCTA 19694 0.18136836509873683 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 19570 0.18022640931158115 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 18894 0.17400090840741003 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 18397 0.1694238759379233 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 18390 0.16935941069187418 No Hit
GTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGG 18377 0.1692396895206401 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 17931 0.16513233241522543 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 17721 0.16319837503375215 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 17665 0.16268265306535928 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 16964 0.15622691913958422 No Hit
GGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCTGGTACTTT 16275 0.14988169706417903 No Hit
GTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCTTTCAA 16267 0.14980802249726577 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 15701 0.14459554688815207 No Hit
CGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTG 15417 0.14198009976273104 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 14734 0.1356901336125108 No Hit
TGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATT 14711 0.13547831923263517 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 14518 0.1337009203058526 No Hit
CGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGGATCGCTGT 13351 0.12295364285737967 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 13250 0.12202350145009966 No Hit
GCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTC 12913 0.11891996031887825 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 12687 0.11683865380357845 No Hit
CGTGGTGGACTTGATTTCACTAAAGATGATGAAAACGTAAACTCTCAACC 12660 0.11659000214024617 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 12658 0.11657158349851786 No Hit
GGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGACTGCTACTT 11872 0.1093330572992893 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 11864 0.10925938273237602 No Hit
GCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGA 11831 0.1089554751438588 No Hit
CCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTCTG 11729 0.10801612441571463 No Hit
GGCCCTGGAGATTTCTTAGTTCATCACGCTATTGCTTTAGGTTTACATAC 11547 0.1063400280184378 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 11307 0.10412979101103977 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
ACTTC 3801790 3.686672 6.557478 40-44
CTGCT 3408015 3.4871852 17.868492 5
AACTT 4145390 3.260897 5.2752223 55-59
AAAAA 3450040 3.2415125 7.1010695 45-49
CTACT 3224065 3.1264405 17.46118 2
CAACT 2650495 3.1187944 7.5300493 9
CTTCT 3529105 2.8203154 9.582026 40-44
TCAAG 2792680 2.812764 5.126427 30-34
ACTGC 2223670 2.7609417 19.547348 4
CTTCA 2843405 2.7573073 5.757437 60-64
TTCAA 3488595 2.7442408 7.703079 9
TCTCT 3399310 2.7165887 7.6894355 20-24
AGCTG 2528015 2.6866984 8.66181 3
TCAAC 2269325 2.6702776 6.687609 8
AGGAA 2529095 2.6457148 5.9786563 40-44
CTGGT 3019510 2.644615 13.114153 1
AGCAC 1747235 2.6323981 5.8333144 35-39
GCAGC 1619750 2.5749888 9.597239 65-69
ACCCA 1450485 2.5530603 29.168175 1
CTGCA 2030460 2.5210493 7.8147826 35-39
GGTTC 2859900 2.5048218 11.611746 3
GCTGC 1892390 2.479275 8.08046 3
CGGTG 2178785 2.443323 16.684694 9
GAAGC 1883595 2.4290707 6.063829 35-39
CAGCA 1611695 2.4281926 6.050966 35-39
ACAAC 1694540 2.419496 5.727738 85-89
GGTGT 3112360 2.333287 12.863389 8
CAACA 1633910 2.3329275 5.7152743 85-89
ATGGT 3259890 2.3160832 12.06266 1
CAAGG 1794805 2.3145678 5.75344 4
AAGCT 2276845 2.2932196 5.0438547 35-39
CCGTG 1747915 2.289994 7.858208 95-97
TATGC 2741780 2.275786 6.67829 30-34
CAAGC 1502400 2.2635279 6.43815 1
CTCCT 1889640 2.2589166 10.607242 70-74
GGTGG 2344235 2.2501943 9.021838 7
TCTGC 2189050 2.2399032 5.880389 85-89
ACATC 1900390 2.2361581 5.4709697 40-44
CTCTG 2169040 2.2194283 8.43039 45-49
TGGTG 2940625 2.20454 8.718528 6
TGCAG 2072100 2.2021658 8.012926 65-69
CAGCT 1746765 2.1688094 7.481133 65-69
GCTCC 1395180 2.135462 9.963785 70-74
CGGAA 1649325 2.1269581 6.2847023 95-97
CCAAG 1400830 2.1105015 5.8817763 75-79
CGTGA 1972665 2.0964894 8.0513525 9
TGGTT 3580445 2.0964038 7.997528 2
CATGG 1962015 2.0851707 10.08354 2
GATGG 2283725 2.0774736 5.4061155 1
GCTAC 1671510 2.0753717 22.309479 1
ATGCA 2057990 2.0727906 6.168182 1
TTTCA 3191000 2.0686407 6.448133 8
ATCCG 1629335 2.0230064 9.594799 95-97
CCTAC 1379270 2.0007083 7.5492606 20-24
AGCTC 1594590 1.9798666 7.9333453 70-74
GTTCC 1933120 1.9780278 5.682069 95-97
CTCTA 2039000 1.9772592 8.210568 25-29
AAGGA 1883105 1.9699373 5.0984917 35-39
TCCTT 2448725 1.95692 6.1947017 40-44
GCAAC 1290615 1.9444509 8.243995 35-39
CCAGC 1045145 1.9411163 6.8649626 30-34
CCCAA 1094090 1.9257543 29.791061 2
GCTGG 1714640 1.9228235 10.626478 4
AATGA 2341605 1.9131588 6.631714 75-79
CTTTC 2392840 1.912259 7.6219873 7
CCTGT 1863585 1.9068773 9.132495 1
TGCTG 2176790 1.906525 13.922547 6
GCTTC 1810885 1.8529531 6.7968345 15-19
GCAGG 1357885 1.8477482 5.6127176 90-94
CTGTA 2211000 1.8352175 7.9676104 8
CCTCC 1022255 1.8279675 6.923455 70-74
CTTGG 2084475 1.8256717 7.5334096 2
TTCGG 2083905 1.8251724 12.869675 7
TCCGT 1782965 1.8243843 7.4398103 95-97
TTCCT 2272275 1.8159087 5.6352553 20-24
TTCCA 1871060 1.8144046 5.87614 95-97
GAAAA 1822010 1.8063482 5.812583 40-44
CTCTC 1499205 1.7921821 5.2258687 15-19
CACAA 1253830 1.7902418 5.3435802 85-89
CATCC 1225105 1.7770833 5.388158 90-94
CTGTT 2597630 1.7768991 11.024589 8
CCTTA 1832015 1.7765417 5.7880874 45-49
CCAAT 1495100 1.7592597 20.001799 3
TGGTA 2475175 1.7585597 9.979257 2
GGAGA 1592910 1.7583133 5.501498 7
ATGAT 2611230 1.7582039 6.2847733 15-19
TACTG 2115075 1.7555959 13.158245 3
TCGGT 2004040 1.7552233 14.564036 8
TGGCT 2000980 1.7525432 6.219168 9
GGCTC 1334200 1.7479742 5.6480803 1
GATCG 1633780 1.7363325 5.3275485 95-97
TAGCT 2068960 1.7173189 6.024447 2
CTATT 2630595 1.7053452 5.4296474 25-29
TGCAA 1686610 1.6987396 6.4224715 35-39
GCACA 1124465 1.6941279 5.0571322 95-97
ACCAG 1120405 1.6880109 6.3970056 30-34
TATTC 2587510 1.6774144 7.576557 5
ATTGC 2009650 1.668089 5.3272433 55-59
CACCC 764425 1.6586603 6.6395407 50-54
ATTCC 1700055 1.6485775 6.152616 20-24
TGTAG 2315200 1.644901 6.8777976 3
CCGCC 717080 1.6417888 9.300164 45-49
AGATC 1618245 1.629883 5.363136 95-97
GCACC 875945 1.6268662 5.971993 50-54
CTATG 1948085 1.6169876 5.769205 4
GTGGC 1441615 1.6166492 7.3263936 8
CGCAG 1008515 1.6032814 6.542125 90-94
CTACC 1105110 1.6030239 7.3954697 20-24
ATGCC 1286920 1.597859 6.2832704 9
GCATG 1503480 1.5978534 5.556973 3
TTCAT 2436755 1.5796838 6.1381702 60-64
CCACA 893040 1.5718776 5.697966 60-64
TACTT 2409105 1.561759 6.858212 5
GCTGT 1775930 1.5554348 14.203597 7
ACGGT 1460245 1.5519046 6.098916 4
CACAC 881650 1.5518296 5.9778566 95-97
TTGGT 2631430 1.5407411 10.67629 7
CAACC 866345 1.5248907 5.051171 75-79
GGTAC 1426075 1.5155898 11.536374 3
TTGCA 1816100 1.5074348 5.418285 65-69
GACGG 1088980 1.4818344 7.963305 3
GTGTT 2523595 1.4776024 8.157428 9
AAACC 1029085 1.4693469 5.3067102 40-44
CACTG 1177990 1.4626099 5.207005 35-39
GCACT 1173585 1.4571406 6.118547 35-39
ACACA 1018235 1.453855 5.1053863 85-89
TGTTT 3170995 1.4500834 8.233056 9
AGCCT 1158610 1.4385474 8.602376 1
TCCTG 1404325 1.4369484 6.659088 75-79
GCTTG 1638715 1.4352561 7.239172 1
AAAAC 1234760 1.4301476 5.11339 40-44
GTAGC 1340085 1.4242023 7.5363803 1
ACTCT 1459510 1.4153161 5.7957397 25-29
GTTTT 3086630 1.4115036 5.082136 10-14
GGTCA 1325865 1.4090896 16.55707 9
GTGTA 1980965 1.4074339 11.196441 2
TCTAT 2144575 1.3902712 5.3514643 25-29
GTATT 2504895 1.3899534 6.906695 4
AACAC 962410 1.374147 5.100401 85-89
GGCTG 1221135 1.3693994 6.0984635 2
ACTTT 2108355 1.3667908 6.720262 6
AACCA 955505 1.364288 5.261369 75-79
CAGGT 1279465 1.3597771 5.637093 95-97
GTCTG 1544590 1.3528174 5.594223 90-94
TCCTC 1103990 1.3197335 6.478589 70-74
GCCTG 1000055 1.310201 9.104806 6
TGTAT 2350985 1.3045497 6.753649 3
CAATT 1657440 1.3037956 13.415378 4
AACGC 865305 1.3036754 5.2224374 90-94
TGGTC 1479060 1.2954235 13.748484 8
ACCGC 697185 1.2948607 5.3641896 45-49
CTGAC 1040600 1.2920244 6.8020887 1
GCGGC 767850 1.2880447 7.3351264 7
GTACT 1543345 1.2810376 8.754596 4
ACATG 1270680 1.2798184 8.738868 1
ATGGG 1399950 1.2735155 9.641387 3
AGGTA 1473055 1.2699409 7.0502367 6
TGACG 1170035 1.2434782 5.8933997 2
CAGGA 964070 1.2432579 5.235855 5
AAGCC 812295 1.2238102 8.770199 1
TACCA 1036535 1.2196738 5.4147787 30-34
GGTCG 1071555 1.2016581 8.147745 6
TACTC 1234315 1.19694 5.4336 25-29
GTAGA 1358645 1.1713066 6.3243613 4
ACACG 777140 1.1708454 5.065412 95-97
TAGGT 1625305 1.154745 5.4086328 5
GCGAC 721315 1.1467068 6.690568 20-24
TATGG 1606385 1.1413027 5.32529 5
TACCC 786460 1.1408042 5.8159122 20-24
ATTGG 1604350 1.1398569 11.65723 6
TGGGT 1512105 1.1336012 6.168235 4
GTTCG 1282100 1.1229177 11.171494 4
TCCTA 1152480 1.1175829 5.720675 20-24
TGGCC 841070 1.1019102 10.044984 1
CGCCT 707830 1.0834044 6.0067306 45-49
ATTCG 1295835 1.0755943 9.541981 6
GGGTC 949665 1.0649687 9.794797 9
CGGTA 1001750 1.0646299 5.5157614 5
CCAGG 658415 1.0467117 5.7884684 1
GGCAG 752560 1.0240494 5.0083137 3
AATTG 1504035 1.0127028 10.594267 5
TTGGC 1138525 0.99716836 6.2582254 3
TGGGG 1038365 0.99671024 7.821408 7
TCGTG 1127920 0.9878802 6.248886 8
TGCGG 864220 0.96914953 5.149893 6
GGCCA 584145 0.9286414 5.238984 1
CGGCT 705945 0.924879 5.469107 8
CGACT 734475 0.9119351 5.0728407 20-24
GGGGT 936080 0.89852846 7.6832185 8
GCCCA 481555 0.89437747 6.508662 2
GTCGC 654625 0.85764325 5.1996293 10-14
CCCTA 561425 0.8143784 6.8476696 2
GGCCC 411440 0.8063222 8.842946 1
GCCTA 633655 0.78675544 8.198501 2
GGCCT 586605 0.7685282 9.04765 5
GCCCT 480605 0.7356139 7.695216 1
GTCGT 773770 0.6777006 6.143599 7
CCTAT 682360 0.66169816 6.2671857 3
GGTAA 704510 0.6073678 6.2817364 7
CCTAG 332355 0.4126569 5.653137 3
CTAGG 379960 0.40381008 5.0253334 4