FastQCFastQC Report
Thu 31 Jul 2014
3L22A_CGATGT_L002_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 3L22A_CGATGT_L002_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 15466762
Filtered Sequences 0
Sequence length 101
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
TGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 223192 1.4430428295204905 No Hit
TGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 221423 1.4316054000184395 No Hit
TGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 139940 0.9047789058886405 No Hit
GCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 137989 0.8921647595016979 No Hit
TGACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 118384 0.7654090752802687 No Hit
TGGCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 95067 0.6146535389889624 No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 84163 0.5441539735336975 No Hit
TGACTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 73396 0.4745401784807964 No Hit
GACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 71108 0.4597471662135875 No Hit
AGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 70713 0.4571933026447294 No Hit
GCTGCGGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 62038 0.40110528629069225 No Hit
TCCTCTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGT 57707 0.3731033037167055 No Hit
TGACTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 52542 0.33970911299986384 No Hit
GACCAGACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 52502 0.3394504939042833 No Hit
TAGATAAGTCTAACTTATAAATTAAATTATAAGTTAAAATTAACCGTTTA 50306 0.3252523055569097 No Hit
TGACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 49275 0.3185863983683204 No Hit
GACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 43803 0.2832073060928978 No Hit
GCTGTAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 43658 0.2822698118714182 No Hit
CAGTAGTTTCACGGATTAAACTTGAAGTTACTAAGGAACCATGCATAGCA 41641 0.2692289439767677 No Hit
GGGTGCATAAGGATGTTGTGTTCAGCTTGGAACACAATCATGAAGTTGAA 41385 0.2675737817650521 No Hit
GACCATACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 38114 0.2464252052239506 No Hit
GGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 38030 0.24588210512323133 No Hit
TGTCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 35896 0.23208477637400768 No Hit
GCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 35767 0.23125072979076033 No Hit
TGATCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 33921 0.21931545852971682 No Hit
TGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 33720 0.21801589757442444 No Hit
TTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 32358 0.20920991736990585 No Hit
GGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 31724 0.20511080470495377 No Hit
TGGCTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 31161 0.20147074093465717 No Hit
TGACTGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 30952 0.20011945616024868 No Hit
ACAGGAGGAGCTGCAATGAAAGCAATAATAAATACAGAAGTTGCAGTTAA 28244 0.18261094338944375 No Hit
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 27241 0.17612606956776086 No Hit
TGCCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 25750 0.16648604277999493 No Hit
TGTCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 25212 0.16300761594443622 No Hit
GTTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 25046 0.16193434669777682 No Hit
TGGCCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 24088 0.15574041935862204 No Hit
GGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 23542 0.15221026870394722 No Hit
AGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 23443 0.1515701864423853 No Hit
TGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 23211 0.15007019568801797 No Hit
AGCGTATTGGAAAATTAATCTACCAAAGTAACCGTGAGCAGCTACGATGT 22735 0.14699262845060912 No Hit
CGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 22494 0.14543444839973615 No Hit
TGACGAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 21902 0.14160688578514366 No Hit
TGGCTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 20462 0.13229659834424295 No Hit
GACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 20405 0.13192806613304064 No Hit
TTGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 19889 0.12859187980005124 No Hit
GACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 19161 0.12388501226048479 No Hit
CTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCA 18354 0.11866737200714667 No Hit
TGGTCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 15931 0.10300152029235336 No Hit
GGCCAGACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 15927 0.10297565838279532 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 17117295 7.930606 13.747884 7
AGCAG 6625910 5.1519904 59.78356 20-24
TAGCA 7844030 4.679403 38.168087 20-24
CACGG 4442470 4.5987115 50.163292 45-49
ACTTG 6754550 4.450831 110.37008 8
GCAGA 5547445 4.3134274 40.052715 25-29
CCTGC 3964065 4.1913714 52.110912 15-19
CTGCG 3493255 3.9942515 55.631413 15-19
TCACG 4719985 3.748641 38.537113 45-49
GTTTC 5118820 3.725701 39.23002 40-44
GAAGT 5710220 3.6837804 31.471937 60-64
CGGAT 4237735 3.6396267 38.870514 45-49
AGAGA 6056365 3.5371985 26.648863 90-94
GAACC 4918880 3.5367627 34.660095 70-74
ACGGA 4327400 3.3647792 37.16829 45-49
CTTGA 5054845 3.33083 32.450207 55-59
AGTAG 5135800 3.3132107 29.654436 35-39
AACTT 7201645 3.2961311 76.79882 7
GCGTT 3374050 3.2008703 43.239567 15-19
TGCGT 3295725 3.1265655 45.71864 15-19
GAGCC 2965370 3.0696619 73.52891 95-97
AGAGC 3939705 3.063326 60.647675 95-97
CCATG 3835510 3.046186 38.786133 75-79
ACCTG 3819485 3.0334587 30.192411 15-19
CTTGT 4161960 3.0292566 122.0342 9
GCAGC 2917885 3.0205066 19.74798 20-24
TTCTC 4481195 3.0160758 31.397694 30-34
CATGC 3729965 2.9623613 38.39776 75-79
AACCA 5878005 2.9356065 23.012156 70-74
GAGAG 3488040 2.9329238 50.33961 95-97
ATAGC 4862310 2.9006395 28.700882 80-84
CACTG 3596990 2.856752 37.86723 85-89
TGAAG 4398980 2.8378727 24.35841 60-64
ACCAT 5131135 2.8305762 31.66865 75-79
CTCAG 3531965 2.8051083 38.544495 35-39
TGCAT 4209950 2.7740967 30.243324 75-79
AGCAC 3835405 2.757725 32.473587 80-84
TTCAC 4521210 2.7549238 23.908356 45-49
GCACT 3443815 2.7350988 29.054659 85-89
GGAAC 3488525 2.71251 37.699028 70-74
AGGAG 3197075 2.6882653 20.768114 95-97
AAGTT 5400965 2.6732152 24.981817 60-64
GACTC 3364215 2.6718802 38.11481 25-29
AGAAG 4540880 2.652085 18.824429 6
TTGAA 5350495 2.648235 22.982767 55-59
TTTCA 5094820 2.5756993 23.724564 40-44
GGATT 3609700 2.5722048 26.626743 50-54
AAACT 6154255 2.5500858 40.99143 6
CAGAC 3482925 2.5042858 34.678898 25-29
GTAGT 3466920 2.4704626 26.606003 40-44
GCTGC 2156465 2.4657412 31.01067 1
AAGGA 4218065 2.463546 21.9835 70-74
ATGCA 4119015 2.457223 29.058615 75-79
CTGAA 4078920 2.4333038 28.39484 85-89
AGGAA 4142970 2.419687 28.497896 70-74
TTGTA 4418980 2.4158955 32.150482 9
TTAGC 3637865 2.397128 32.240852 20-24
TCAGT 3621445 2.3863082 32.279373 35-39
CAGTA 3969370 2.3679512 28.782768 35-39
TCTCA 3866735 2.3561304 22.921038 35-39
GTTAG 3297315 2.349605 34.749912 20-24
GTTAC 3565735 2.3495991 29.830221 60-64
CATTC 3848270 2.3448792 29.843294 30-34
ACAGG 2993765 2.3278086 13.643872 90-94
AGTTT 4216425 2.3051567 26.964306 40-44
TAGAG 3557790 2.2952037 30.336905 90-94
CTCAT 3732710 2.2744641 22.848429 30-34
CATAG 3810095 2.272935 28.769474 80-84
GCATA 3797795 2.2655969 22.17742 80-84
GGAGG 1857730 2.2489188 17.938564 90-94
TAACC 4071455 2.2460065 26.558992 10-14
AACCT 4027650 2.2218418 25.443342 10-14
TGTAA 4445365 2.2002397 24.612293 10-14
TAAGG 3405435 2.196916 29.27008 65-69
GTAAC 3655595 2.1807668 29.05208 10-14
CGTTA 3290425 2.1681864 24.360168 20-24
CTAAG 3628770 2.1647642 28.61966 65-69
GATGA 3282920 2.1178792 10.04752 15-19
GAAGA 3625240 2.1173086 9.157297 50-54
ACTGA 3545870 2.11531 28.81157 85-89
AGACT 3541970 2.112983 29.014383 25-29
CTGCA 2647555 2.1027045 18.131685 3
GTTGT 2650145 2.0859199 13.233299 35-39
CAGCA 2891980 2.0793858 15.475087 20-24
TGAAT 4166775 2.062351 22.872837 85-89
TCATT 4076550 2.06091 24.717133 30-34
ACCAG 2846800 2.0469005 12.757065 55-59
ATTCT 4047550 2.0462492 24.78509 30-34
CAGAA 3773345 2.0379062 13.630397 5
TAGTT 3675240 2.009286 26.560316 40-44
ACTCA 3635805 2.0056815 25.260298 25-29
GGCTC 1740845 1.9905139 19.069893 65-69
AGTTA 4021295 1.9903455 23.743544 60-64
GGAGC 1757550 1.9674746 19.865078 95-97
TGTTG 2493995 1.9630146 11.951397 35-39
GAATA 4367990 1.9572698 16.729729 90-94
CAGGC 1884305 1.9505758 14.99517 65-69
AATAG 4304280 1.9287217 21.787743 90-94
GTAGG 2075570 1.9277476 19.611235 7
TTAAA 5541045 1.9049445 17.160418 50-54
CCAGA 2638245 1.8969456 15.865536 55-59
TGCAG 2205395 1.8941284 20.969898 4
TTTCC 2779050 1.8704443 10.856035 40-44
AAGAG 3202040 1.8701402 10.094117 50-54
TTACT 3689260 1.8651148 18.814926 65-69
CCATC 2536415 1.8627895 12.238581 75-79
CCAAA 3659855 1.8278129 34.141884 4
AGGGA 2167335 1.8224069 17.832603 9
TAAAC 4292095 1.7784781 19.224075 50-54
ATTAA 5142225 1.767835 17.127306 50-54
GGGGG 998805 1.7407796 7.3036494 1
GATTA 3499785 1.7322234 23.920387 50-54
GGATG 1854220 1.7221621 15.868874 10-14
TACTA 3762235 1.7219427 22.199928 65-69
TCCTC 2111565 1.7129371 9.571005 1
TGACC 2137875 1.6979135 52.82119 1
TTCCG 1929825 1.6929522 14.198529 40-44
GGAAG 2013330 1.6929116 8.729587 6
AGCTG 1956395 1.680272 7.952219 1
CAGAG 2155135 1.67573 12.924452 25-29
TTGTT 2771800 1.6738305 10.750678 35-39
CCTCC 1708305 1.6702867 5.758391 1
TGATG 2343500 1.6699344 12.277996 30-34
CAGGA 2130980 1.6569481 12.535088 90-94
GATGT 2324955 1.6567196 14.741473 30-34
ATAGA 3693160 1.6548823 21.639027 90-94
AAGAA 4077340 1.6540644 9.284764 45-49
GAGGA 1964940 1.6522228 18.468914 95-97
TAGGG 1770160 1.644089 19.522491 8
ATGAT 3289130 1.6279595 8.656108 15-19
ACCAA 3252665 1.6244532 17.862673 3
ACTAA 3890235 1.6119634 20.267656 65-69
TCCGT 1767880 1.5508848 14.684947 40-44
GCTCA 1950630 1.5492023 16.349854 65-69
AGGCT 1801565 1.5472945 13.879361 65-69
GAGCT 1793790 1.5406169 9.470708 95-97
TGTTT 2545855 1.5373871 12.784395 35-39
GAGAT 2378440 1.5343806 13.463189 25-29
CATCA 2766605 1.5261899 8.330057 80-84
CAAAC 3011195 1.5038577 39.63155 5
GGGAT 1606250 1.4918528 18.907972 10-14
GAGAA 2554195 1.4917686 12.495453 50-54
CTACA 2701210 1.4901148 9.842115 85-89
AACAG 2758935 1.4900441 13.282161 60-64
TGCTG 1568640 1.4881264 6.5976233 1
TACAG 2481785 1.4805235 9.765999 90-94
CTCAC 1991700 1.4627409 11.701999 65-69
GAAAC 2684145 1.4496515 9.042138 60-64
AGCCA 1996995 1.4358752 14.3605175 95-97
GACCA 1990280 1.431047 23.150106 2
TACCA 2581360 1.4239999 9.352507 75-79
AGAAA 3441300 1.3960406 6.5551443 60-64
ATACC 2529455 1.3953667 8.906367 75-79
AAAGA 3416480 1.3859719 7.4794917 45-49
GAGCG 1204470 1.3483337 6.513736 90-94
GGCCA 1294785 1.3403225 20.661371 2
TAAAG 2981620 1.3360456 8.23155 45-49
GCTCC 1254540 1.3264774 5.6145625 1
GTAAA 2954305 1.3238062 7.5072513 45-49
TCTTG 1815195 1.3211783 6.2957287 4
GCTGG 1063620 1.3151684 6.6931205 1
AGATG 2035775 1.3133205 10.362119 25-29
ATGTT 2393495 1.3085448 8.887434 30-34
TCCAA 2363810 1.303989 5.216685 3
CTCTT 1935840 1.3029202 6.726896 3
GCCAA 1795185 1.2907703 16.71934 3
CAACA 2553560 1.2753047 6.4659543 8
TCTAC 2091590 1.2744755 11.977916 85-89
AAGTA 2823690 1.2652783 20.254087 8
GATAC 2076700 1.2388678 8.413786 75-79
CCGTA 1559005 1.2381715 16.101377 40-44
AGAAC 2282625 1.2327988 9.685579 50-54
TGGCC 1055840 1.2072669 35.008312 1
AAACA 3182915 1.1940179 6.796349 60-64
TCAAT 2576000 1.179013 7.5999265 80-84
CAATA 2844545 1.1786696 8.498275 80-84
CCTCT 1451895 1.1778018 7.766868 2
CGGAA 1513890 1.1771284 7.4004207 5
ATCAA 2744610 1.1372602 6.89338 80-84
GATAG 1744150 1.1251872 13.228644 15-19
CCAAC 1674760 1.1135317 5.115616 5
GCCCA 1154390 1.1050309 7.3834295 2
TGATA 2214595 1.0961169 8.667102 15-19
CCCAA 1640285 1.0906096 7.9150357 3
CCGAA 1508120 1.0843654 33.4896 4
CGTAA 1807905 1.0785164 9.420728 40-44
TGCCA 1357725 1.0783136 5.1853867 1
GGATA 1636775 1.0559173 10.024716 70-74
TCAAC 1908800 1.0529841 7.2595663 7
TATCT 2030205 1.0263753 7.9918427 85-89
ATCTA 2174400 0.9952042 7.6367097 85-89
GTTGC 1042895 0.9893664 6.0355463 1
TTGAC 1499920 0.9883546 6.0879846 6
CCATA 1769900 0.9763604 5.053689 3
GAACT 1621000 0.96701705 28.530664 6
GGTGC 781550 0.9663883 8.3513975 2
CTCCG 905560 0.95748645 5.4264116 2
AATAT 2756950 0.94780624 5.5220413 80-84
GTCCA 1187285 0.94294906 5.7475057 2
CGAAC 1309555 0.9415935 31.738504 5
GTGCA 1090020 0.93617606 6.440079 3
GACCT 1151335 0.9143973 10.8854265 2
GGCTG 732250 0.9054287 8.806959 1
TCCCC 923455 0.90290344 5.1325917 1
GGGTG 670660 0.89678246 9.980716 1
ATTGG 1256610 0.8954368 5.5867944 6
TGTCC 1015175 0.8905692 9.521353 1
CTAAA 2133660 0.88410616 6.609251 4
ATATC 1923480 0.8803602 6.5228086 85-89
CCTAA 1568430 0.86522 10.397605 4
TAAGT 1743755 0.8630739 5.043554 5
AACAA 2285715 0.8574481 5.2494893 9
ACCTA 1552115 0.8562199 7.606763 3
TGCCC 805430 0.8516147 8.194296 1
GCTGT 895400 0.84944177 7.607613 1
GGGGT 622260 0.8320637 5.4640236 1
CCCCC 704520 0.83024734 5.2135115 1
ACCGA 1153145 0.829132 20.57309 3
CTGTC 944790 0.8288235 8.950917 4
TGGCT 869455 0.82482857 7.2393413 1
GGCCT 700665 0.8011531 6.4967012 2
GTCAA 1332860 0.79512554 7.4720073 6
TGTCA 1203040 0.79272896 7.193462 5
AAGTC 1302865 0.7772319 6.0373864 6
GGGGC 478810 0.7716787 5.0833597 1
GGGCC 514015 0.7660539 9.648697 1
CCGTC 704815 0.7452304 5.6919713 4
AGTCT 1103815 0.7273458 7.7772098 7
GGCCG 479385 0.71444356 19.428755 2
GACCG 679065 0.7029476 29.06725 2
TGCGG 555200 0.68650603 11.340906 3
GCCCC 525090 0.6691719 6.572335 1
CCCCG 518115 0.660283 6.1186757 2
GCCGA 629760 0.6519086 13.995402 3
TCTAA 1379060 0.6311839 5.752756 9
GACTG 734875 0.6311557 8.320121 2
TGACT 956775 0.63045555 12.595862 1
GGCCC 455180 0.6273023 7.0940037 1
CTAAC 1131980 0.62445354 9.437676 5
GACTT 912620 0.60136014 8.26966 6
TAACT 1308885 0.5990654 8.177134 6
GACCC 617900 0.59147996 6.15456 2
GCGGA 509760 0.5706465 9.476605 4
CCCGA 584600 0.5596038 5.4956098 3
GTCTA 849050 0.5594714 7.797348 8
GCCCG 394890 0.5442142 5.9775753 2
CCCGT 487115 0.515047 5.853804 3
GGACC 497275 0.51476413 5.403398 1
GTCCG 435750 0.4982445 6.20823 2
GACTA 657590 0.3922892 5.8004847 2
Produced by FastQC (version 0.10.1)