FastQCFastQC Report
Thu 31 Jul 2014
3L22A_CGATGT_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 3L22A_CGATGT_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 15466762
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[OK] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 350107 2.2636088924107063 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 149694 0.9678431723459635 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 117511 0.7597647135192227 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 113367 0.7329717752170751 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 102493 0.6626661740834959 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 90963 0.5881192197823953 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 83191 0.5378695295110897 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 74924 0.4844194279319744 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 67520 0.43654903334001005 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 62242 0.4024242436781532 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 57738 0.3733037335157805 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 57371 0.3709309033138287 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 55155 0.35660340541866486 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 48124 0.31114463389298935 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 45902 0.29677834313348844 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 45831 0.2963192942388329 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 44789 0.28958226679895893 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 43130 0.2788560398097546 No Hit
TGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTC 42922 0.27751122051273563 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 41458 0.26804576161448657 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 41373 0.2674961960363779 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 40575 0.2623367450795454 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 39922 0.25811478834419255 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 38889 0.2514359502008242 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 38610 0.24963208200914966 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 37865 0.24481530135396148 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 37673 0.24357392969517472 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 33825 0.21869477270032345 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 30719 0.19861299992849185 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 30634 0.1980634343503831 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 30384 0.19644706500300452 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 29541 0.1909966675636439 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 29099 0.18813892655747855 No Hit
ACCCAATTTGGGAAGCTGCTTCCGTTGATGAATGGCTTTACAACGGTGGT 28166 0.18210663615306166 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 27102 0.17522736821061835 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 26278 0.16989981484165853 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 26048 0.1684127550420702 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 25086 0.1621929657933574 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 24602 0.15906367473683244 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 24380 0.15762833875636026 No Hit
GCTAAAAACTATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGA 23699 0.15322534865410098 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 23660 0.1529731950359099 No Hit
CTGCTGCTATCATCCCTACTTCTGCAGCTATCGGTTTGCACTTCTACCCA 23648 0.15289560930723572 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 23272 0.15046458980877833 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 22926 0.14822753463200636 No Hit
TACAACATCGTAGCTGCTCACGGTTACTTTGGTAGATTAATTTTCCAATA 22152 0.14322325513252226 No Hit
GTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATATTGATGGTAT 22144 0.14317153131340613 No Hit
TACCATGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTC 21802 0.1409603380461922 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 21700 0.14030085935246175 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 21089 0.13635045266746848 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 20521 0.13267806151022432 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 20210 0.13066729804208535 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 19073 0.12331605025020752 No Hit
TTCATGCGTTGGAGAGACCGTTTCTTATTCTGTGCAGAAGCTATTTACAA 18417 0.11907469708268609 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 18135 0.11725143245884304 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 17871 0.11554454642801124 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 17819 0.1152083416037565 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 17517 0.11325576743212316 No Hit
ATACACTTGGGTAACTTATTATTATTTTACAAACCAAGATTTACCATGAC 17233 0.11141957185350107 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 17138 0.11080535150149722 No Hit
CCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGATT 16716 0.10807692004312214 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 16567 0.1071135639120845 No Hit
ACCTCCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCC 16165 0.10451444200149974 No Hit
GCTGCTATCATCCCTACTTCTGCAGCTATCGGTTTGCACTTCTACCCAAT 15855 0.10251014401075027 No Hit
GCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCT 15621 0.10099722230160393 No Hit
GCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATG 15610 0.10092610205031927 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 5926805 3.8882167 5.5043354 90-94
CTGCT 4747870 3.3863692 11.256308 4
ACTTC 4849195 3.291016 7.5981455 60-64
AACTT 5393110 3.0220516 6.516058 60-64
AGCTG 4080770 3.0073745 6.8781643 80-84
CTACT 4105865 2.7865384 11.42272 2
CTTCT 4873830 2.7777824 6.4436884 20-24
CAAGC 2719205 2.7500615 9.634505 75-79
GGAAG 3535650 2.6923065 7.3988447 15-19
CAACT 3273175 2.6452215 8.478199 55-59
GCTGC 2959875 2.6419008 9.680417 3
CTTCA 3882470 2.6349263 7.5495787 60-64
TCAAG 3675350 2.5773237 6.3565574 10-14
TTCAA 4588360 2.5711067 7.86888 55-59
TCTCT 4486870 2.5572393 9.096012 20-24
ACAAC 2643795 2.5442107 9.300853 85-89
TGCTA 4292380 2.5277605 6.817929 4
AGGAA 3486210 2.5260017 7.2884755 10-14
ACCCA 2148005 2.503562 47.73997 1
CTGGT 4015915 2.4854064 10.343637 1
AAGCT 3422345 2.399905 6.50119 15-19
TCAAC 2968800 2.3992405 8.295887 55-59
CTGAA 3419225 2.397717 6.9810863 80-84
CAGCA 2360300 2.3870835 6.4878483 2
GCTAC 2805485 2.3827362 14.688023 1
ACTGC 2792415 2.3716354 9.368037 2
CAACA 2448675 2.3564403 8.8419 85-89
ACATC 2905800 2.348327 7.428954 90-94
GAAGC 2675275 2.34772 8.269116 15-19
CGGTG 2995385 2.319921 11.259079 9
TGGTG 4314825 2.3171465 6.999614 8
GCAGC 2177425 2.3142955 7.7802944 1
GGTGT 4266965 2.2914445 9.717291 1
CCAAG 2241070 2.2665007 8.781661 75-79
GGTGG 3343060 2.2466843 6.358734 7
TATGC 3802485 2.239264 7.840075 30-34
CAGCT 2549785 2.1655667 6.4628873 2
TCCTT 3776070 2.152127 6.4402337 40-44
TGAAA 3692115 2.1377046 5.0743184 65-69
CAAGG 2434570 2.1364863 7.8605413 10-14
CTTTA 4506815 2.1208007 6.832417 5
GCTTC 2937475 2.095124 8.902392 15-19
CCAGC 1699755 2.0820184 7.1173115 1
CATGG 2805115 2.0672646 14.659905 2
CTGCA 2422460 2.057428 5.007444 95-97
ATGGT 4025060 2.0567784 5.564503 1
CTTGG 3275850 2.027388 6.5298686 6
CTCCT 2448235 2.0123856 7.931422 70-74
CTCTG 2815565 2.0081728 8.401142 20-24
GCTCC 1925320 1.9804718 5.972785 70-74
GCTGG 2551450 1.9760941 7.444076 4
CACAA 2030320 1.9538435 8.193691 85-89
AAGGA 2663345 1.9297788 7.097973 10-14
CGTGA 2602390 1.917864 12.286612 9
TTCCA 2825070 1.9172978 6.443131 75-79
GTTCC 2678970 1.9107479 5.873017 70-74
CCTTA 2780650 1.8871511 9.431357 95-97
CCCAA 1611330 1.8780518 48.019485 2
AGCTC 2207915 1.8752118 5.210163 65-69
TTTCA 3974490 1.870301 6.3758187 8
CCGTG 2082550 1.8588253 5.0061007 60-64
CATCC 1851070 1.8118132 8.287207 90-94
GAAAG 2487270 1.8022002 5.074003 7
GCCTT 2472135 1.763225 5.914086 35-39
GGTAT 3441390 1.7585269 5.94505 6
CCGCC 1183200 1.7553111 6.096782 45-49
CCAAT 2158455 1.7443588 33.452095 3
TATTC 3686190 1.7346342 6.250542 5
TTCGG 2779325 1.7200942 8.978382 7
CTTTC 3000540 1.7101228 7.4538198 7
CACCC 1204810 1.7007457 6.079748 50-54
TCGGT 2747620 1.7004724 10.866215 8
AGAAA 2460000 1.6960555 8.205757 9
TTGGT 3917010 1.6808823 19.201021 7
CCACA 1441000 1.6795273 7.575836 1
CCTGT 2351375 1.6770939 6.6144533 1
TCTGG 2700490 1.6713041 5.5197554 45-49
GCACC 1353965 1.6584625 5.422159 50-54
TACTT 3520280 1.6565608 8.409223 3
GAACA 1958905 1.6357468 7.521635 80-84
AGCTT 2768990 1.6306441 5.4715266 15-19
ACACA 1691605 1.627887 7.9044886 85-89
TCCAA 1974350 1.595574 6.8979287 75-79
GCTTG 2571850 1.5916902 6.141797 1
TGTAG 3097640 1.582873 5.1417737 9
TTTAG 3821480 1.5604115 5.6194844 6
AACAC 1620405 1.559369 7.960202 85-89
GCTGA 2112455 1.5568001 6.430392 80-84
TGGTA 3041025 1.5539433 7.9913745 2
ACGGT 2096740 1.5452188 8.7515135 4
CATGC 1818675 1.544625 6.270199 2
GTGTA 3015580 1.540941 9.036495 2
AATGA 2653225 1.5361959 5.0694027 75-79
TGAAC 2174885 1.5251288 6.5937734 80-84
CTACC 1553805 1.5208526 5.0663767 25-29
ACTTT 3206730 1.5090116 8.700532 4
ACATG 2146660 1.5053362 14.930899 1
GCAAC 1479210 1.4959955 7.216918 1
TTAGG 2907090 1.4855033 6.1807585 35-39
GTAGC 2010930 1.4819801 5.300721 1
AAGCC 1464100 1.4807141 6.6731977 1
CATGA 2110125 1.4797162 5.841691 65-69
AACAT 2212575 1.476363 5.7223954 90-94
ATGGG 2300050 1.4708219 12.514779 3
AGCCT 1727385 1.4670912 5.5805554 2
ATGCC 1718295 1.459371 9.350068 9
GGTCG 1878800 1.4551276 12.692835 6
CTGTA 2444045 1.4392856 6.6412897 8
TTCAT 3026110 1.4240161 5.536873 60-64
GCGGC 1258015 1.4051949 7.1333623 7
GACGG 1507035 1.3898768 11.082875 3
GGCTG 1791685 1.3876572 5.7348433 9
GGTAC 1869105 1.3774604 9.802775 3
GGAAT 2228225 1.3558344 5.377497 40-44
ATCTC 1997405 1.3555841 6.0979304 45-49
TCATG 2291340 1.3493584 5.061207 60-64
TTCTC 2356335 1.3429656 5.615974 20-24
GTCAA 1911335 1.3403156 5.9952273 10-14
GGGTC 1723885 1.3351462 12.656692 5
CAATT 2382560 1.3350774 23.450333 4
TGGGT 2473585 1.328364 9.399293 4
GACTG 1795515 1.3232273 7.7926354 1
GTATT 3223870 1.3163915 6.012713 4
TGGTC 2091175 1.2942057 23.44087 8
GAATC 1833835 1.285969 5.878276 40-44
GGTCA 1735630 1.2790942 27.61424 9
TGCCT 1739775 1.2408766 5.8602524 30-34
CTGAC 1451520 1.2327954 9.967547 1
GTATG 2407060 1.2299913 6.1275973 7
ATCCT 1811940 1.2297142 5.6857457 90-94
TGTAT 2977125 1.215639 5.8100185 3
GTACT 2037095 1.1996348 7.8115735 4
TGACG 1620950 1.1945794 8.6089525 2
TGGGG 1754130 1.178853 5.0768886 8
CACAT 1450205 1.1719855 5.0907016 2
GCCTG 1291150 1.152444 6.2115474 6
ATTGG 2188770 1.1184466 19.949924 6
TGGCC 1245255 1.1114794 8.5406275 1
ATTCG 1862140 1.0966047 7.5464535 6
CTTAT 2320740 1.0920856 6.3380466 95-97
GCTCA 1278900 1.086187 6.1425858 1
TGCGG 1400295 1.0845263 5.221294 6
TCGTG 1740325 1.0770683 10.277336 8
AATTG 2116730 1.0292135 18.479248 5
CGGTA 1355695 0.9990964 8.267482 5
TTATG 2437450 0.9952753 5.66518 95-97
TAGAA 1701430 0.9851142 6.5964036 8
CGGCT 1103250 0.98472977 5.505031 8
GCCTA 1128905 0.95879424 5.288806 3
CTACA 1024450 0.8279109 7.1548476 3
TTAGA 1667090 0.81058586 5.795507 7
GGCCT 887850 0.7924698 6.068291 5
GTCGT 1268545 0.7850888 10.024745 7
GGCCC 557420 0.7175562 5.872276 1
CCCCG 465905 0.6911834 5.4837704 1
Produced by FastQC (version 0.10.1)