FastQCFastQC Report
Wed 6 Aug 2014
3L15C_GCCAAT_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 3L15C_GCCAAT_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 18359396
Filtered Sequences 0
Sequence length 101
%GC 46

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[OK] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GCGAGTCGGGCTGTTTGGGAATGCAGCCCTAAGTGGGAGGTAAATTCCTT 376940 2.0531176515828737 No Hit
GATACCGTCCTAGTCTCAACCATAAACGATGCCGACTAGGGATTGGCGGA 261603 1.4248998169656562 No Hit
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 218126 1.188089194219679 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 144313 0.7860443774947715 No Hit
CAGACCTCTGATCAGGAAAGACTACCCGCTGAGTTTAAGCATATCACTAA 112932 0.6151182751327985 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 87058 0.47418771292911815 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 84490 0.4602003246729903 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 84004 0.45755317876470447 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 59944 0.3265031158977126 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 59471 0.32392677841907214 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 58604 0.3192044008419449 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 58164 0.31680780783855855 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 57677 0.3141552151279922 No Hit
CAACACGGGGAAACTTACCAGGTCCAGACATAGTAAGGATTGACAGATTG 56559 0.3080656901784786 No Hit
CATGGAGAGTTTGATCCTGGCTCAGGATGAACGCTGGCGGCATGCTTAAC 56402 0.30721054222045213 No Hit
GATCAGGAAAGACTACCCGCTGAGTTTAAGCATATCACTAAGCGGAGGAG 55618 0.30294024923260005 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 53099 0.289219754288213 No Hit
CAAACGAATAAGGGCTTATGGTGGATACCTAGGCACCCAGAGACGAGGAA 52862 0.2879288621477526 No Hit
TGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCATGCATGTGTAAGT 52470 0.2857937156538265 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 50430 0.27468223900176236 No Hit
ACCTCCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCC 48648 0.26497603733804753 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 48626 0.26485620768787815 No Hit
CTGATCAGGAAAGACTACCCGCTGAGTTTAAGCATATCACTAAGCGGAGG 48123 0.2621164661408251 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 40896 0.2227524260602037 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 39041 0.21264860783001793 No Hit
TGGGTCGGGGGGAGCGGTCCGCCCCTTGTGGGTGTGCACTGGTCCACTCG 36559 0.19912964457000654 No Hit
GCAGACCTCTGATCAGGAAAGACTACCCGCTGAGTTTAAGCATATCACTA 35858 0.1953114361714296 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 35247 0.19198343997809078 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 34268 0.18665102054555607 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 30918 0.16840423290613699 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 28903 0.15742892631108343 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 28656 0.1560835661478188 No Hit
GAAACATCTTAGTAGCCAGAGGAAAAGAAAGCAAAAGCGATTCCCATAGT 28532 0.15540816266504628 No Hit
TGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTC 28446 0.15493973766892985 No Hit
GCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATG 26119 0.14226502876238414 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 26074 0.14201992265976507 No Hit
TGGCAAGAGACCGATAGCGAACAAGTACCGCGAGGGAAAGATGAAAAGGA 25089 0.13665482241354782 No Hit
AAGGCTAAATATTGGCAAGAGACCGATAGCGAACAAGTACCGCGAGGGAA 25052 0.13645329072917214 No Hit
CCACGAGGCGCGATCTGCGAGTCGGGCTGTTTGGGAATGCAGCCCTAAGT 24832 0.13525499422747894 No Hit
ACCTGGTTGATCCTGCCAGTAGTCATATGCTTGTCTCAAAGATTAAGCCA 24444 0.13314163494267459 No Hit
AGCAAGCGACGAAATGCTTCGGGGAGCTGAAAATAAGCATAGATCCGGAG 24402 0.1329128692468968 No Hit
AACACGGACCAAGGAGTCTAACATGTATGCAAGCAGGTGGGCGGCAAACC 24153 0.13155661547907133 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 23506 0.12803253440363724 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 23490 0.12794538556715046 No Hit
CTAAGGCTAAATATTGGCAAGAGACCGATAGCGAACAAGTACCGCGAGGG 22866 0.12454658094416614 No Hit
AAGTGGGAGGTAAATTCCTTCTAAGGCTAAATATTGGCAAGAGACCGATA 22707 0.12368053938157877 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 22326 0.12160530771273739 No Hit
CAACGCGAAGAACCTTACCAGGGCTTGACATGCCGCGAATCCTTTTGAAA 21999 0.11982420336703888 No Hit
TAGTAGCCAGAGGAAAAGAAAGCAAAAGCGATTCCCATAGTAGCGGCGAG 21855 0.11903986383865788 No Hit
ACCAATAGGGGTGCTTCGTGCACCCCCAGAAGCATGTGTCGAGAGCTCCC 21211 0.11553212317006509 No Hit
ACAGACCTCTGATCAGGAAAGACTACCCGCTGAGTTTAAGCATATCACTA 21012 0.11444820951626077 No Hit
AAATCCCTTAACGAGGATCCATTGGAGGGCAAGTCTGGTGCCAGCAGCCG 20823 0.11341876388526072 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 20676 0.11261808395003844 No Hit
CCAGACCTCTGATCAGGAAAGACTACCCGCTGAGTTTAAGCATATCACTA 20486 0.11158319151675795 No Hit
GCTCCACGAGGCGCGATCTGCGAGTCGGGCTGTTTGGGAATGCAGCCCTA 20314 0.1106463415245251 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 20308 0.11061366071084255 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 20080 0.10937178979090598 No Hit
ACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGACGG 20044 0.10917570490881073 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 20024 0.10906676886320225 No Hit
GTAGGTGGCTTTTTAAGTCTGCTGTCAAATCCCAGGGCTCAACCCTGGAC 19634 0.10694251597383704 No Hit
GTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATATTGATGGTAT 19523 0.10633792092071004 No Hit
TGGTGGTAGTAGCAAATATTCAAATGAGAACTTTGAAGACTGAAGTGGAG 18987 0.10341843489840297 No Hit
GCAGCGTGCCGGCCCCAAGTTCCCTGGAATGGGATGCCCAAGAGGGTGAC 18699 0.10184975584164098 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 18640 0.101528394507096 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 18623 0.10143579886832878 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGCT 4667730 3.1304262 7.691796 4
CTTCT 4889720 3.0381634 8.801376 45-49
AAAAA 6723725 2.9281385 6.9136353 70-74
AGCAC 3841520 2.7208922 7.144012 70-74
AACTT 5212960 2.6749222 5.6773295 10-14
ACTTC 4187250 2.6736913 5.8710203 60-64
TCCTT 4036205 2.5078428 10.285098 45-49
TCTCT 4030720 2.504435 7.753425 20-24
TGAAA 5567150 2.4778478 5.671092 65-69
GAAGA 5133660 2.4662633 5.1923976 90-94
CTGGT 4331825 2.4520447 12.070883 1
CGGAA 4046815 2.419253 5.974427 95-97
AAGAG 4969050 2.387183 5.4122515 65-69
ATCGG 4062500 2.3632302 7.350353 95-97
CTGAA 4265360 2.362398 5.8252196 80-84
TGGTG 4788545 2.2878144 7.3810687 4
AGCTG 3927470 2.2846808 5.525209 1
AGAGC 3766660 2.2517717 7.0298634 95-97
GAAAC 3906915 2.2237532 5.0386434 60-64
GGTGG 4305245 2.2201672 6.0977736 7
TCGGA 3786660 2.2027693 6.2616596 95-97
CTACT 3447045 2.201047 8.974966 2
AGATC 3937275 2.1806858 8.172 95-97
TGCTA 3989990 2.1503735 5.3966208 4
ATGCA 3851470 2.1331623 5.6421857 20-24
TTCAA 4151860 2.1304407 8.700282 9
CTTCA 3323410 2.1221023 6.045298 60-64
GCAGC 2818550 2.0967627 7.868787 20-24
TCAAC 3173880 2.082707 6.766465 8
GATCG 3568810 2.076042 7.7427115 95-97
CTCTG 3063980 2.0548668 8.561178 6
TTCCT 3294520 2.0470068 8.651045 45-49
ATTCC 3195065 2.04015 6.2167444 40-44
TATGC 3757645 2.025153 5.83193 30-34
CCTTA 3117690 1.9907435 6.369351 95-97
CGGTG 3231365 1.9743042 6.7260866 9
GGTGT 4063880 1.9415926 6.8376284 1
GTCTG 3415590 1.9334065 5.7509756 90-94
CAAGC 2728395 1.932482 5.330308 30-34
GAAAG 3931135 1.8885579 5.273203 15-19
TTTCA 3778745 1.8867705 9.497133 8
CACAC 2247550 1.8860701 7.345564 95-97
CTGTT 3561140 1.8675659 5.89291 10-14
GCACC 2114535 1.8637117 6.8866606 70-74
CATGG 3199395 1.8611465 6.381966 2
CAACT 2823530 1.8528064 7.7621446 9
AGGAA 3834405 1.8420877 5.6218047 10-14
CAAGT 3303045 1.8294134 5.0179434 85-89
GCTGC 2523670 1.8268416 5.508312 3
GAGCA 3032145 1.8126663 5.310129 95-97
GCAAG 3015915 1.802964 5.743796 65-69
CCCTA 2189855 1.7881691 7.4719152 25-29
CAGCA 2523635 1.7874535 5.0624948 70-74
GCTTC 2653060 1.7792821 6.2273784 15-19
GCACA 2504010 1.7735535 6.440769 95-97
ACTTT 3550020 1.7725656 9.1836605 6
GAGAA 3666705 1.761523 5.2060804 80-84
CTTTC 2822660 1.753823 10.658165 7
CTAAG 3163605 1.7521836 5.565021 50-54
GCGAA 2915155 1.742728 5.58689 80-84
GCTGG 2828900 1.7284056 6.734999 2
ACCCA 2058610 1.7275181 22.348833 1
CAGCC 1959065 1.7266834 7.870916 20-24
TGGGA 3501265 1.7190852 6.0314713 30-34
TGCAG 2950705 1.716479 6.3402042 20-24
CTACC 2091745 1.7080555 7.7898145 20-24
CCAGC 1935030 1.7054994 5.796368 70-74
GGAGG 3217520 1.7051567 6.000957 35-39
CCGCC 1531365 1.6795703 7.1069303 65-69
TACTT 3348645 1.6720169 9.044199 5
CACCC 1597760 1.6684594 5.8716545 50-54
ACTCC 2028360 1.6562973 5.394018 60-64
ATGCC 2401220 1.6549503 5.3787327 30-34
GCGAG 2629470 1.6510168 25.578794 1
TTGGT 3713930 1.6439188 13.790634 7
GGAAA 3412395 1.6393499 5.195985 9
CAACA 2425640 1.6357588 5.5293083 1
TCTGA 3034860 1.6356138 6.9475102 7
TGTTT 3978355 1.6314716 5.307481 10-14
CCAAT 2426555 1.5923104 17.104532 3
TTCGG 2795675 1.5825015 5.863507 7
ACACG 2218135 1.5710723 5.7102714 95-97
GAACA 2730295 1.55404 8.59888 80-84
GCTAA 2784745 1.5423495 6.20331 55-59
ACTGC 2229055 1.536292 6.3430476 2
GGGAA 3037890 1.5328504 5.836843 15-19
CAAGA 2681680 1.5263691 5.331802 65-69
GGAAT 3233570 1.5116088 5.6711063 15-19
CTCTA 2363075 1.508898 5.472698 95-97
TTTGG 3357765 1.4862671 5.102679 95-97
ACCGC 1684765 1.4849205 6.8043275 90-94
AGAGA 3082205 1.4807231 5.2499127 70-74
GCTGT 2541415 1.4385768 5.965598 10-14
GGGAG 2709450 1.4358999 5.3308077 35-39
AGCCC 1622355 1.4299134 8.522568 25-29
GCTAC 2067140 1.4246982 8.979301 1
ATTGG 3052010 1.3883141 12.111101 6
TTGGG 2897525 1.3843453 5.015085 95-97
GTGTA 3036280 1.381159 6.3581953 2
AGCGA 2294850 1.3718992 5.5734973 80-84
CAACC 1625895 1.3643978 5.8129134 15-19
TGGTA 2999220 1.3643008 8.752442 2
TCGGT 2404790 1.3612398 6.29323 8
CATCC 1663980 1.3587556 5.161442 90-94
CGCGA 1825090 1.3577126 5.503605 90-94
TGCCG 1862915 1.3485323 5.626836 30-34
GCCCT 1561625 1.3393232 8.150632 25-29
CGAGG 2132210 1.3387924 6.445263 95-97
CAGGA 2225360 1.330357 5.3645263 4
GTTAC 2452745 1.3218875 5.9536695 9
TACCC 1616645 1.3201033 5.4992003 20-24
CCCAA 1570120 1.3175932 22.474136 2
GAGTC 2260015 1.3146919 23.227343 3
ATCTC 2024060 1.2924262 5.571855 45-49
GGCTG 2100350 1.283275 26.260735 9
AGGGA 2537650 1.2804404 5.203851 95-97
TGGTC 2224255 1.2590473 15.141455 8
AACAC 1858885 1.2535607 7.757312 2
CCAGT 1788110 1.2323873 6.035323 3
ACGGT 2117985 1.2320704 5.270546 4
CCTTC 1531340 1.2167732 7.1325774 45-49
CCTCC 1190885 1.2100929 6.4561896 2
CCACA 1440555 1.2088666 5.07951 60-64
GGCAA 2021790 1.2086595 5.3268423 65-69
CGCCA 1369000 1.206611 5.3364844 65-69
GGGCT 1943410 1.1873876 25.035704 8
ACAAG 2085595 1.187087 5.342925 85-89
AGACC 1667260 1.1808957 9.239467 2
TACCG 1713025 1.1806378 19.938215 3
ACATG 2110385 1.1688508 5.271317 1
CGAAC 1631775 1.1557622 5.7860956 80-84
GCCAG 1550310 1.1532996 6.405069 2
AAGGC 1928165 1.152689 5.503947 50-54
AGGTA 2452685 1.1465654 5.5081906 35-39
CCTAG 1662330 1.1456982 18.83478 9
CCGAC 1297360 1.1434689 5.38203 30-34
CCGCG 1222305 1.131511 6.8254523 90-94
GAGGG 2107835 1.1170679 5.4321375 95-97
AATGC 1996200 1.1056086 5.2296205 20-24
AAGAC 1927090 1.0968686 5.3959646 9
CACCT 1336690 1.0915005 6.0215807 70-74
GACCG 1431510 1.0649225 5.681161 70-74
TCGGG 1740775 1.0635813 24.92044 6
CTAAA 1991185 1.0500106 5.0098753 55-59
CCCCG 952070 1.0442111 11.416925 1
GTCCT 1546485 1.0371544 18.721283 7
ACCGA 1442755 1.0218822 5.4850507 70-74
CCTAA 1553410 1.0193509 5.7916875 25-29
AGGCT 1751710 1.0190016 5.182817 50-54
GTACT 1881810 1.0141865 9.032861 4
GAGGT 2044530 1.0038432 5.08624 35-39
ACCGT 1446785 0.9971419 19.219885 4
TGGCA 1708100 0.99363303 5.107209 65-69
GGTAC 1692240 0.9844069 9.649413 3
TCCTA 1539375 0.98293954 17.80358 8
TGATC 1818185 0.97989637 7.6897645 9
CCGTC 1111955 0.9536649 23.570236 5
CAATT 1856185 0.95246285 13.471968 4
CCGAT 1362230 0.9388656 5.6230326 75-79
CTCCC 920780 0.93563133 6.0977273 3
GGCTA 1594765 0.927704 5.3400817 55-59
ACCTC 1134800 0.92664325 10.558414 4
GGTCA 1588170 0.9238675 15.333469 9
CCCGC 825885 0.90581405 5.5125012 25-29
CTGAC 1313515 0.9052906 6.2979407 1
GTACC 1310350 0.90310913 5.191989 85-89
CGAGT 1546170 0.8994353 23.274294 2
CAGAC 1266785 0.89724517 9.632285 1
TCCCC 876785 0.8909267 5.718436 4
CGGGC 1120325 0.87535244 31.555286 7
CGGTA 1502795 0.8742033 5.6320677 5
CGGGG 1324580 0.87352806 7.6688137 6
AATTG 2015000 0.87269306 10.80812 5
CCTCT 1089805 0.865938 10.446272 5
CACGG 1162200 0.86457855 7.7022524 4
GTCGG 1411840 0.862608 24.937805 5
CGTCC 996825 0.8549241 23.3134 6
TGGCC 1171295 0.8478804 5.8121705 1
TCCGC 983255 0.84328574 5.1343694 65-69
GCAAC 1189505 0.8425089 5.1959124 1
AGTCG 1430370 0.8320723 23.472399 4
TGCCA 1199350 0.8266067 6.3440495 1
CGGTT 1417395 0.8023214 6.0761366 4
TAGCG 1373240 0.79883873 5.0527363 75-79
CCGGT 1087580 0.78728056 8.07701 3
GGTTG 1629640 0.77859014 6.785145 5
CTCCG 876960 0.7521221 5.102668 65-69
CTGAT 1355080 0.73030967 6.901532 8
ATACC 1095330 0.7187579 18.244663 2
CCCGG 720810 0.6672675 9.722884 2
GACCT 941885 0.64915866 8.779016 3
GATAC 1101710 0.6101894 15.312113 1
Produced by FastQC (version 0.10.1)