FastQCFastQC Report
Thu 31 Jul 2014
3L15B_GCCAAT_L003_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 3L15B_GCCAAT_L003_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 15156367
Filtered Sequences 0
Sequence length 101
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
TGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 177086 1.1683934547111454 No Hit
TGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 171609 1.1322568264545192 No Hit
GCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 144330 0.9522730612157912 No Hit
TGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 109187 0.7204035109469176 No Hit
TGACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 106074 0.6998642880579495 No Hit
TGGCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 82000 0.5410267513316351 No Hit
AGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 75272 0.4966361661735956 No Hit
GCTGCGGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 73657 0.48598057832724684 No Hit
CAGTAGTTTCACGGATTAAACTTGAAGTTACTAAGGAACCATGCATAGCA 69784 0.46042696115764414 No Hit
GACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 60922 0.4019564846905594 No Hit
TGACTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 60894 0.4017717438486413 No Hit
TGACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 60326 0.39802414391258806 No Hit
GGGTGCATAAGGATGTTGTGTTCAGCTTGGAACACAATCATGAAGTTGAA 60059 0.39626250802715457 No Hit
TGACTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 53357 0.3520434679366104 No Hit
TAGATAAGTCTAACTTATAAATTAAATTATAAGTTAAAATTAACCGTTTA 50443 0.3328172246027033 No Hit
GCTGTAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 50015 0.3299933288762406 No Hit
GACCAGACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 46883 0.3093287461302567 No Hit
TCCTCTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGT 44846 0.2958888498807135 No Hit
GGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 42709 0.2817891649100342 No Hit
GACCATACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 41424 0.2733108798434348 No Hit
TGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 40448 0.26687134192514606 No Hit
GCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 40250 0.2655649602572965 No Hit
GACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 38744 0.2556285421169862 No Hit
TTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 35692 0.2354917903479112 No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 35576 0.23472643543139327 No Hit
TGACTGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 35413 0.23365097981594135 No Hit
TGGCCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 31922 0.21061775556107873 No Hit
TGTCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 31654 0.20884952178843388 No Hit
GTTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 29655 0.19566034525292242 No Hit
ACAGGAGGAGCTGCAATGAAAGCAATAATAAATACAGAAGTTGCAGTTAA 29177 0.19250655516589166 No Hit
TGATCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 28932 0.19089007279910813 No Hit
TGGCTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 28801 0.19002574957441978 No Hit
GGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 28071 0.18520929191012594 No Hit
TGTCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 25695 0.16953271189593125 No Hit
TGCCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 23299 0.15372417413750936 No Hit
AGCGTATTGGAAAATTAATCTACCAAAGTAACCGTGAGCAGCTACGATGT 22876 0.15093326784710348 No Hit
TGACGAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 22716 0.14987760589328564 No Hit
TGGCTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 22523 0.1486042136614929 No Hit
TGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 21817 0.14394610529027174 No Hit
AGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 21312 0.14061417224853423 No Hit
GACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 21246 0.1401787116925844 No Hit
TTGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 21118 0.13933418212953014 No Hit
GACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 20515 0.1353556561410792 No Hit
GGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 20229 0.13346866039862984 No Hit
CGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 19747 0.13028847876275362 No Hit
GCTGCTGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 19371 0.12780767317128175 No Hit
GCAGATGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 18332 0.12095246835867725 No Hit
GCAGGAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 18303 0.12076112962954777 No Hit
TAGATGAGTCTAACTTATAAATTAAATTATAAGTTAAAATTAACCGTTTA 17725 0.11694755082138088 No Hit
GCAGTAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 17297 0.1141236550949182 No Hit
GACCACACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 17097 0.11280407765264591 No Hit
CTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCA 16902 0.11151748964643045 No Hit
GTGTCAAACTCAAATTTAATTTCTTTCCAAACTTCACAAGCAGCAGCTAA 16225 0.10705072000433877 No Hit
CTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGA 15837 0.10449073976633054 No Hit
TGGTCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 15596 0.10290064894839243 No Hit
TGCCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 15311 0.10102025109315445 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 10478190 5.4218383 10.53045 7
AGCAG 6779015 5.1759925 59.67587 20-24
TAGCA 7838815 4.710299 38.401134 20-24
CACGG 4517295 4.6739635 47.684814 45-49
ACTTG 6503450 4.4562087 104.67793 8
GCAGA 5547740 4.2358747 38.763607 25-29
CCTGC 3796355 4.189136 52.003445 15-19
GTTTC 5156880 4.029326 40.621147 40-44
CTGCG 3302215 3.8961513 54.52544 15-19
TCACG 4782505 3.894335 37.62367 45-49
GAAGT 5759255 3.7003026 31.959612 7
CGGAT 4246260 3.6970665 36.86617 45-49
GAACC 4942215 3.529196 32.798717 70-74
AGAGA 6217990 3.503461 23.841333 90-94
CTTGA 5058245 3.4659443 32.25819 55-59
ACGGA 4362770 3.3311126 34.62649 45-49
AGTAG 5179670 3.3279212 31.823973 9
AACTT 6881810 3.2544057 72.44413 7
GCGTT 3231000 3.2078297 42.37063 15-19
GCAGC 3025255 3.1301763 21.574543 20-24
TGCGT 3118105 3.0957444 45.424873 15-19
CTTGT 3952825 3.0885377 119.43414 9
TTCTC 4209475 3.0760999 31.979065 30-34
ACCTG 3737910 3.043734 29.12396 15-19
AGAGC 3975090 3.0351071 56.118755 95-97
CCATG 3723705 3.0321667 37.959885 75-79
ATAGC 4974930 2.989407 27.396679 80-84
GAGCC 2835060 2.9333851 67.930504 95-97
CATGC 3588875 2.9223766 37.434994 75-79
AACCA 5918295 2.9167423 21.324478 70-74
ACCAT 5137725 2.8873272 31.932455 75-79
TTCAC 4481605 2.8719869 23.941357 45-49
TGCAT 4086470 2.8000772 29.470636 75-79
CACTG 3402610 2.7707036 36.55112 85-89
TGAAG 4274790 2.7465386 22.611612 60-64
AAGTT 5420660 2.7409058 24.360088 60-64
CTCAG 3358280 2.7346058 37.498405 35-39
GAGAG 3319100 2.7096944 44.949776 95-97
AGGAG 3304850 2.6980608 21.707674 90-94
TTGAA 5320215 2.6901164 22.006851 55-59
TTTCA 4972640 2.6815119 23.814852 40-44
GCACT 3283425 2.6736522 27.550478 85-89
AGCAC 3741820 2.6720035 30.028996 80-84
AGAAG 4707125 2.65218 19.634676 6
GACTC 3214380 2.61743 37.156902 25-29
GGAAC 3374750 2.5767286 35.134323 70-74
GGATT 3473155 2.5445955 25.445803 50-54
GCTGC 2147355 2.5335784 35.476326 1
TTGTA 4270855 2.4625235 33.655502 9
GTAGT 3347310 2.4523957 25.48774 40-44
ACAGG 3203630 2.4460726 14.149715 60-64
AAACT 5862825 2.4313772 36.197685 6
TTAGC 3531760 2.4199865 31.907635 20-24
GTTAC 3493725 2.3939245 29.079058 60-64
ATGCA 3961425 2.3803976 27.798782 75-79
CAGAC 3315795 2.3677826 32.71645 25-29
CAGTA 3938330 2.3665197 27.577358 35-39
AGTTT 4094415 2.3607905 27.049433 40-44
CATTC 3680335 2.3585017 29.822464 30-34
TCAGT 3420465 2.3437264 31.724133 35-39
CTGAA 3886755 2.3355286 26.974167 85-89
TCTCA 3628535 2.325306 22.482403 35-39
GTTGT 2775160 2.3184958 15.454883 35-39
AAGGA 4081640 2.299757 19.736076 70-74
GTTAG 3127325 2.2912242 33.860287 20-24
GATGA 3561410 2.2881944 11.257453 15-19
GCATA 3798255 2.2823496 20.892315 80-84
CTCAT 3542735 2.270322 22.404707 30-34
CATAG 3733005 2.2431414 27.538118 80-84
AGGAA 3958620 2.230443 26.047653 70-74
TAGAG 3454795 2.2196946 28.399197 90-94
ACCAG 3101835 2.2149954 14.156017 55-59
GGAGG 1859825 2.20001 18.578234 90-94
TAACC 3913795 2.1994965 25.745031 10-14
CGTTA 3172245 2.173644 23.63195 20-24
GAAGA 3845035 2.1664443 9.81205 50-54
TGTAA 4283960 2.166144 23.726465 10-14
GTAGG 2319175 2.159022 23.928083 7
GGCTC 1825985 2.1544068 21.755617 65-69
CAGCA 3016970 2.1543941 17.368078 20-24
AACCT 3833155 2.1541777 24.286188 10-14
CTGCA 2633380 2.1443286 20.066439 3
TAAGG 3326715 2.1374037 27.249657 65-69
TGTTG 2557380 2.1365523 14.045011 35-39
CTAAG 3535280 2.1243296 27.364496 65-69
TCATT 3930015 2.119273 25.040884 30-34
TTTCC 2892235 2.1135187 13.085149 40-44
CAGAA 3976010 2.0951824 14.396509 6
GTAAC 3470495 2.0854003 27.8146 10-14
TGAAT 4106820 2.076575 21.852608 85-89
ATTCT 3838020 2.0696645 25.175125 30-34
CAGGC 1996715 2.0659647 16.728657 65-69
TAGTT 3549980 2.046876 26.601059 40-44
AGACT 3372070 2.0262575 27.772404 25-29
ACTGA 3357720 2.0176346 27.216858 85-89
AGTTA 3968080 2.0064225 23.061949 60-64
CCAGA 2809035 2.0059094 18.066002 55-59
GAATA 4471925 1.9829545 15.433872 90-94
CCATC 2599280 1.9795114 13.793171 75-79
AAGAG 3496680 1.9701675 10.789647 50-54
GGAGC 1774760 1.9634478 20.259005 95-97
AGGGA 2392010 1.9528233 21.01998 9
ACTCA 3469145 1.9496095 24.079006 25-29
TTACT 3565050 1.9224645 18.72338 65-69
TGCAG 2207990 1.9224179 22.97014 4
AATAG 4334680 1.922097 20.433777 90-94
TTAAA 5432865 1.895912 16.501461 50-54
TTCCG 2023235 1.8786585 16.710865 40-44
TAGGG 2010425 1.8715931 23.852446 8
TTGTT 2813975 1.8501608 12.843748 35-39
GGATG 1983310 1.8463506 18.483206 10-14
TGATG 2470155 1.8097512 13.961928 30-34
GATGT 2452025 1.796468 17.160732 30-34
ATGAT 3529570 1.784694 9.816589 15-19
ATTAA 5094310 1.7777661 16.534702 50-54
TAAAC 4247825 1.7616191 18.056868 50-54
CCAAA 3573455 1.7611234 28.159563 4
CAGAG 2299965 1.7560961 14.070807 25-29
TACTA 3686410 1.7433021 21.744146 65-69
GGAAG 2132895 1.741283 10.193726 6
TGTTT 2625225 1.7260596 15.696037 35-39
GATTA 3386385 1.7122939 23.147358 50-54
TCCGT 1836960 1.7056943 17.19638 40-44
TCCTC 1960625 1.7026412 8.720223 1
CAGGA 2210535 1.6878133 12.952292 90-94
AGGCT 1908875 1.6619893 15.613566 65-69
GAGAT 2582925 1.6595211 15.181304 25-29
GCTCA 2024040 1.6481507 18.937937 65-69
CCTCC 1595685 1.6467661 5.8706903 1
TGACC 2020655 1.6453944 45.483925 1
AGCTG 1887890 1.6437184 8.509608 1
AAGAA 4169260 1.6212633 10.003483 45-49
ACCAA 3259940 1.6066123 14.620505 3
GGGAT 1724690 1.6055901 21.620726 10-14
GAGCT 1843805 1.6053352 10.115426 95-97
TCTCC 1842290 1.5998769 5.156632 1
ATAGA 3600100 1.5963674 20.2039 90-94
CATCA 2838845 1.5953901 9.068988 80-84
GAGGA 1950805 1.592626 18.570608 95-97
CTACA 2831005 1.5909841 10.870251 85-89
ACTAA 3803620 1.5774025 19.275833 65-69
TACAG 2621005 1.5749469 10.4894905 90-94
CTCAC 2047970 1.559655 13.742422 65-69
ATACC 2735445 1.5372807 9.958815 75-79
AACAG 2910065 1.5334761 14.656345 60-64
TACCA 2725110 1.5314726 10.337428 75-79
GAGCG 1365555 1.5107373 7.5925164 90-94
GAAAC 2830040 1.4913067 9.73546 60-64
GAGAA 2613720 1.472673 13.498633 50-54
AGCCA 2056165 1.468291 14.264069 95-97
GATCG 1682135 1.4645748 5.4220853 95-97
CGTCG 1239135 1.4620057 5.1636086 95-97
TGCTG 1460780 1.4503044 7.621752 1
TAAAG 3263750 1.447222 9.009907 45-49
GGGGG 836155 1.4331514 6.745665 1
ATGTT 2474810 1.4269458 10.606151 30-34
GTAAA 3180350 1.4102404 8.052064 45-49
CAAAC 2824210 1.3918694 33.545914 5
AAAGA 3574335 1.3899201 7.9074874 45-49
AGATG 2156555 1.3855796 11.749075 25-29
AGAAA 3561095 1.3847716 6.8681417 60-64
CCGTA 1698405 1.3829902 18.765854 40-44
TCTAC 2149860 1.3777137 13.6223135 85-89
GACCA 1926040 1.3753699 18.651192 2
TCTTG 1741780 1.3609389 5.438493 4
CTGCT 1464950 1.3602674 5.3895116 2
AAGTA 3043935 1.3497509 23.120256 8
AGCGT 1536985 1.338198 5.724695 90-94
CTCTT 1816050 1.3270898 5.9651985 3
GATAC 2202095 1.3232262 9.27067 75-79
GCTCC 1194160 1.317711 5.4719563 1
CGGAA 1706575 1.3030239 8.387827 5
GGCCA 1248440 1.2917383 16.965607 2
GCCAA 1789515 1.2778784 14.121859 3
AGAAC 2398005 1.2636431 10.777941 50-54
CAATA 3027470 1.2555246 9.171711 80-84
CAACA 2515675 1.2398125 6.657879 8
TCAAT 2609015 1.2338026 8.303415 80-84
GCTGG 966380 1.2191336 7.0686965 1
TGTAG 1633075 1.196467 5.307072 3
GATAG 1861055 1.1957217 14.950696 15-19
CGTAA 1971820 1.1848552 10.871738 40-44
TGATA 2343150 1.1847918 9.78432 15-19
AAACA 3247900 1.1812027 7.2030983 60-64
CCTCT 1345485 1.1684428 6.894291 2
TGGCC 981180 1.1576551 31.77099 1
ATCAA 2787085 1.1558344 7.359081 80-84
GCCCA 1192025 1.1535046 7.2401266 2
CTTTT 1845125 1.1345983 5.39606 6
TATCT 2085855 1.1248039 9.077328 85-89
GGATA 1736670 1.1158048 11.245163 70-74
CCAAC 1651415 1.1029034 6.1950603 5
TGCCA 1347875 1.0975579 5.6616874 1
CCCAA 1623340 1.0841534 8.382105 4
CCTTT 1466670 1.0717782 5.088116 4
ATCTA 2242000 1.0602411 8.283837 85-89
TCAAC 1875695 1.0541136 7.733061 7
CCGAA 1475950 1.0539641 29.034384 4
TTGAC 1514595 1.0378109 5.0955887 6
CCATA 1804545 1.0141282 5.6461945 3
AATAT 2883615 1.0062977 6.049569 80-84
GTTGC 991940 0.9848267 6.5630016 1
GTCCA 1183030 0.96332663 5.351709 2
GGTGC 755635 0.95326895 10.714864 2
CTAAA 2276670 0.94415975 5.9465117 4
ATATC 1994765 0.94332385 7.200587 85-89
CAACT 1672140 0.9397184 5.378338 6
CTCCG 845970 0.9334963 5.564852 2
GTGCA 1071210 0.9326643 8.308154 3
TGTCC 999210 0.92780834 9.75509 1
TAAGT 1826175 0.9233883 5.1066113 5
GAACT 1535545 0.9227002 25.84917 6
CCGTT 992225 0.9213225 5.3459015 4
GGCTG 722295 0.911209 9.438599 1
GACCT 1118245 0.91057307 10.144821 2
CCTAA 1618395 0.9095146 10.539442 4
ACCTA 1602480 0.9005707 7.093937 3
GGGTG 656540 0.8855997 12.429068 1
TGCCC 797735 0.8802708 8.694312 1
CTGTC 930185 0.8637157 9.714783 4
CGAAC 1206495 0.86154836 27.612349 5
GCTGT 864165 0.85796785 9.001788 1
ACCCA 1244840 0.831371 5.224594 3
GCAGG 741355 0.82017374 5.738384 1
GGCCT 695070 0.82008535 7.810441 2
ACCGA 1139630 0.8138007 17.019814 3
TGTCA 1187535 0.8137071 7.789644 5
AAGTC 1333705 0.8014156 6.0803394 6
AACAA 2184845 0.79458857 5.2877665 9
GGGGT 588590 0.79394263 5.2394223 1
TGGCT 793005 0.78731817 7.7932324 1
GTCAA 1282765 0.7708061 7.8792014 6
CCGTC 696375 0.76842386 5.832659 4
GGGCC 511545 0.76690817 10.105979 1
GCCAG 739175 0.764811 5.4152603 2
TGGGC 590875 0.7454165 5.0121703 1
AGTCT 1070310 0.73338383 8.549866 7
TGCGG 529625 0.6681467 13.279894 3
GGCCG 444910 0.667009 17.524271 2
TCTAA 1395350 0.6598605 6.2612467 9
CAGGG 583795 0.6458624 5.1600666 2
TGACT 930285 0.63743776 12.912969 1
GACCG 615985 0.63734853 23.940489 2
GACTG 713530 0.6212451 8.8610735 2
CTAAC 1103155 0.6199572 9.659709 5
TAACT 1306165 0.6176851 8.720316 6
GCCGA 594470 0.61508733 12.706887 3
GCCCC 468195 0.6139606 6.153492 1
TCCGA 752840 0.6130283 5.1087546 3
CCCCG 463215 0.60743016 5.8306603 2
GGCCC 431130 0.60449797 7.1387024 1
GACTT 873575 0.59857965 8.99771 6
GTCTA 850570 0.5828164 8.575043 8
GACCC 576505 0.5578752 7.2490473 2
GCGGA 493575 0.5460505 10.852359 4
CCCGA 546490 0.5288301 5.82656 3
GCCCG 366290 0.51358426 6.7234297 2
CCCGT 442105 0.48784634 5.921388 3
GTCCG 412850 0.4871052 6.5517263 2
GGACC 466285 0.48245665 5.1399155 1
GACTA 619075 0.37199864 5.016782 2
Produced by FastQC (version 0.10.1)