FastQCFastQC Report
Thu 31 Jul 2014
3L15B_GCCAAT_L003_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 3L15B_GCCAAT_L003_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 15156367
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 247217 1.6311098827311319 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 135579 0.8945349502291677 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 126028 0.8315185294734551 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 124921 0.824214668330478 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 108127 0.7134097505028745 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 93527 0.6170805972169979 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 90127 0.5946477806983692 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 83864 0.5533252130936127 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 77150 0.5090269983565323 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 71053 0.4687996800288618 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 64149 0.4232478667216226 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 58369 0.3851120786399537 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 56731 0.3743047393877438 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 53517 0.35309912989042824 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 53454 0.3526834629961124 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 49185 0.3245170824908106 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 46613 0.3075473165831891 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 45653 0.3012133448602821 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 45240 0.2984884174419899 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 41083 0.27106100030436053 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 41043 0.2707970848159061 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 40919 0.26997894680169726 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 39158 0.25836006742248985 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 39079 0.2578388343327923 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 37349 0.24642448945713705 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 36578 0.24133751841717743 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 33845 0.2233054926685267 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 33563 0.22144488847492277 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 32560 0.2148272076019273 No Hit
GCAGCTATCGGTTTGCACTTCTACCCAATTTGGGAAGCTGCTTCCGTTGA 31285 0.2064149014074415 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 29478 0.19449251921651145 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 28693 0.18931317775559275 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 28411 0.18745257356198883 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 27426 0.18095365465879784 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 27005 0.1781759441428147 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 26109 0.17226423720143488 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 25053 0.16529686830623724 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 24946 0.16459089437462157 No Hit
ATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGATTTCACTAAA 24259 0.16005814586041628 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 23279 0.15359221639328213 No Hit
GGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTATTT 23049 0.152074702334669 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 23006 0.15179099318458045 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 22668 0.14956090730714028 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 22504 0.14847885380447703 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 22291 0.14707350382845705 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 21968 0.14494238625918732 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 21677 0.14302240108068115 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 21535 0.14208550109666782 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 20664 0.13633874133557206 No Hit
GTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAG 20235 0.133508247721898 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 20012 0.1320369188737644 No Hit
CGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTC 19688 0.1298992034172833 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 19524 0.12881714991462004 No Hit
ATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTC 19463 0.128414678794727 No Hit
TTCATGCGTTGGAGAGACCGTTTCTTATTCTGTGCAGAAGCTATTTACAA 19317 0.12745138726186822 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 19163 0.12643531263131857 No Hit
GTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCTTTCAA 19020 0.12549181476009388 No Hit
CAAGGTGAAACAGGTGAAATTAAAGGGCATTATCTAAATGCTACTGCAGG 18795 0.12400729013753758 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 17783 0.11733022827963985 No Hit
GGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCTGGTACTTT 17774 0.11727084729473759 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 17624 0.11628116421303336 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 17580 0.11599085717573347 No Hit
GGTCCTTACGAGTTAATCGTTCTTCACTTCTTACTTGGTGTAGCTTGCTA 17486 0.11537065577786551 No Hit
ACCCAATTTGGGAAGCTGCTTCCGTTGATGAATGGCTTTACAACGGTGGT 17360 0.11453932198923396 No Hit
GTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCATCTCTGCT 17343 0.11442715790664082 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 16470 0.10866720237112232 No Hit
GTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGG 16042 0.10584330664465964 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
ACTTC 5091460 3.513985 6.5306892 60-64
CTGCT 4779555 3.4904635 12.866188 5
AAAAA 5020825 3.3554764 6.154261 45-49
AACTT 5653445 3.1869693 5.4168806 60-64
CTACT 4331900 2.9897573 14.059023 2
CAACT 3520190 2.92703 7.4969044 55-59
AGCTG 3770265 2.8669257 8.191887 3
GGAAG 3578820 2.833566 5.7437196 15-19
TCAAG 3907220 2.8078523 5.105107 10-14
TTCAA 4873810 2.7474725 7.238485 55-59
CTTCA 3946075 2.7234716 6.386853 60-64
CTTCT 4738065 2.7142818 7.1181817 40-44
TCAAC 3193655 2.6555169 7.119313 55-59
CTGGT 4163395 2.6277778 11.3696375 1
TCTCT 4581450 2.624562 8.303372 20-24
AGCAC 2463750 2.6115563 5.9022837 35-39
AGGAA 3485185 2.6078415 6.3030033 40-44
GCTGC 2800180 2.6068935 8.411643 3
ACTGC 2943195 2.589514 13.610624 4
ACCCA 2094955 2.5694027 35.72679 1
ACAAC 2444200 2.4485073 6.809907 85-89
GAAGC 2654775 2.4320688 6.671478 15-19
CAAGC 2260660 2.3962824 7.278819 2
CGGTG 2974155 2.39302 13.306514 9
CAGCA 2253520 2.3887143 6.310668 35-39
GCAGC 2106585 2.3627617 7.9596496 1
GGTTC 3742730 2.3622696 6.9748573 3
AAGCT 3285230 2.3608708 5.1173544 15-19
TATGC 3941560 2.3510995 6.8597465 30-34
GGTGT 4274560 2.3317273 10.440109 1
CTGAA 3233525 2.323714 5.6076484 80-84
CTGCA 2617950 2.3033535 6.705017 95-97
CAAGG 2513105 2.3022833 6.185148 10-14
GGTGG 3301345 2.29572 9.594492 7
TGGTG 4203510 2.2929702 8.240196 6
CAACA 2278185 2.2821996 6.6262703 85-89
GCTAC 2570725 2.2618034 18.245731 1
ACATC 2713155 2.2559824 5.4746222 90-94
ATGGT 4238070 2.1848233 8.224404 1
CCAAG 2037975 2.1602383 6.9032526 75-79
CATGG 2840425 2.1598713 13.081437 2
TCTGC 2953410 2.156847 5.127374 85-89
CTCTG 2936825 2.1447356 7.751623 45-49
CAGCT 2429445 2.137501 6.5743413 65-69
ATGCA 2923990 2.101272 5.626966 1
CCGTG 2248580 2.0933688 5.6650705 60-64
TGCAG 2720140 2.068406 5.6150317 65-69
TCCTT 3598550 2.0614913 6.234718 40-44
TGGTT 4814085 2.059958 5.04466 2
CGTGA 2702860 2.0552664 11.109647 9
CTCCT 2420240 2.0450728 8.7127 70-74
GCTCC 1892955 2.0390708 7.35475 70-74
CCAGC 1532090 1.9882907 6.973854 1
CGGAA 2163810 1.9822903 5.29554 95-97
TTTCA 4232120 1.9802461 5.8920293 8
GCTGG 2458920 1.9784592 9.546764 4
CTTGG 3128295 1.9744616 7.004937 2
AAGGA 2588490 1.9368762 5.547678 10-14
AGCTC 2200770 1.9363056 5.9896607 70-74
GCTTC 2641430 1.9290112 7.8317156 15-19
GGTAT 3737125 1.9265745 5.4080863 6
CCCAA 1547465 1.8979219 36.217903 2
TGCTG 3001385 1.8943608 9.961163 6
CCAAT 2271230 1.8885226 24.690828 3
TAGCT 3138215 1.8719126 5.90184 2
TTCCA 2700155 1.8635724 5.2354884 75-79
CCTTA 2676610 1.8473222 6.7427793 95-97
CACAA 1841455 1.8447 6.3236094 85-89
CTGTT 3713220 1.8384422 8.031822 8
CTCTA 2658825 1.8350475 5.937725 25-29
TGGCT 2895800 1.8277197 6.9002147 9
GAAAA 2543020 1.7983199 5.299501 40-44
CTTTC 3138145 1.7977401 6.924068 7
CATCC 1747780 1.779265 6.448907 90-94
CCTGT 2432785 1.7766399 8.390102 1
TACTG 2975075 1.7746013 9.332406 3
ATCCG 2012300 1.7704839 5.8300047 95-97
AATGA 2998630 1.7600967 6.3026967 75-79
GCAGG 1813340 1.7577841 5.2873178 90-94
TATTC 3756530 1.7577133 6.4361362 5
TTCGG 2778485 1.7536749 10.284544 7
GGCTC 1878520 1.7488524 5.1815014 10-14
CACCC 1147225 1.7226571 6.0669336 50-54
ACCAG 1610635 1.7072608 5.9272265 30-34
TCGGT 2703425 1.7062999 12.2639885 8
CCTAC 1660140 1.6900464 5.451811 80-84
CCGCC 1060535 1.6850524 8.473411 45-49
CTATG 2812735 1.6777672 5.0173573 5
GCACC 1285140 1.6678079 5.461098 50-54
TGGTA 3227840 1.6640263 8.9579 2
GCAAC 1557355 1.6507845 5.7999315 1
CCACA 1342845 1.6469611 5.4693995 1
GTGGC 2035115 1.6374636 8.212535 8
TTGGT 3825220 1.6368206 13.726182 7
ACGGT 2140985 1.6280142 8.156134 4
CCTCC 1303455 1.6245863 5.098329 1
CTACC 1581010 1.6094909 6.09676 20-24
TGTAG 3121750 1.6093343 6.07079 9
CTGTA 2691920 1.6057023 6.8047957 8
GCTGT 2542925 1.6049984 10.142588 7
TACTT 3396095 1.5890627 6.402115 5
ATGCC 1803855 1.5870875 8.591838 9
GAACA 1816900 1.5730445 5.6669 80-84
ATTCC 2237340 1.5441502 5.4371743 80-84
TGAAC 2144250 1.5409263 5.262693 80-84
GCACT 1733720 1.5253805 5.7969413 35-39
GCTTG 2407660 1.5196241 6.791063 1
GGTAC 1992725 1.5152767 10.135455 3
ACACA 1504695 1.5073466 6.0347466 85-89
CGCAG 1333655 1.4958375 6.0486493 90-94
TCCAA 1794165 1.4918442 5.3375115 75-79
GTAGC 1954130 1.4859289 7.05956 1
TTCAT 3168010 1.4823396 5.2356014 60-64
GACGG 1523445 1.4767708 10.504563 3
GTGTA 2860530 1.4746695 9.680151 2
CACAC 1200635 1.4725444 5.454899 95-97
ATCTC 2092930 1.4444824 5.1597476 45-49
AACAC 1439095 1.4416311 6.106744 85-89
GTGTT 3335405 1.4272275 5.157611 9
ACATG 1981905 1.4242599 12.382669 1
GGCTG 1762870 1.418414 6.267533 9
ACTTT 3014485 1.410504 6.2891707 6
GCGGC 1187605 1.4094546 7.9980774 7
AACCA 1385810 1.3882521 5.025999 75-79
GGTCA 1825205 1.3878938 20.319584 9
AGCCT 1563050 1.3752198 7.834345 2
TGTTT 4077730 1.3687413 5.8904095 9
ATGGG 2075235 1.3638204 11.8952465 3
GTATT 3332010 1.3474505 5.9878736 4
AGGTA 2154815 1.3383255 5.8083067 6
ACCGC 1029335 1.3358334 5.551891 45-49
TGGTC 2105110 1.3286659 17.080126 8
GGTCG 1646065 1.324432 11.278829 6
CAATT 2341890 1.3201741 16.746756 4
GTCTG 2060790 1.3006928 5.1584325 90-94
CTGAC 1471595 1.2947549 9.186844 1
GTATG 2492320 1.2848487 5.708702 7
AAGCC 1208680 1.2811917 8.730987 1
GTACT 2143540 1.2785994 7.8182063 4
TGACG 1628480 1.2383032 7.964536 2
GCCTG 1323075 1.2317479 7.932404 6
TGGGT 2217550 1.20965 8.313695 4
TGTAT 2968440 1.2004242 5.900252 3
GGGTC 1458835 1.1737859 11.2811575 5
GCGAC 1045795 1.1729716 6.143377 20-24
ATTGG 2216015 1.1424071 14.42981 6
TGGCC 1223855 1.1393766 9.878364 1
CGCCT 1038210 1.1183486 5.491498 45-49
ATTCG 1838255 1.0965 8.055305 6
CGGTA 1429505 1.0870018 7.5803976 5
TCGTG 1687810 1.065282 8.8307085 8
GTAGA 1698330 1.054809 5.0195303 4
TGCGG 1305815 1.0506651 5.750373 6
GTTCG 1648540 1.0404961 6.5927362 4
TGGGG 1495145 1.0397079 6.7650876 8
TTGGC 1623820 1.0248939 5.495936 3
AATTG 2083825 1.0152462 13.167614 5
CGGCT 1070795 0.9968819 6.0537724 8
GGGGT 1350490 0.93911636 6.335992 9
GCCCA 680285 0.88284904 5.579712 2
GCCTA 944655 0.8311367 7.5529284 3
GGCCT 867310 0.8074427 7.696835 5
GGCCC 581580 0.7986255 6.931882 1
CTACA 934765 0.777255 5.13042 3
GTCGT 1196630 0.7552676 8.678349 7
GCCCT 665065 0.7164008 5.9791446 1
CCCTA 702190 0.71483946 5.134966 2
CCTAT 1008535 0.696063 5.7270837 4
GGTAA 966785 0.60045666 5.361236 7
Produced by FastQC (version 0.10.1)