FastQCFastQC Report
Thu 31 Jul 2014
3L15A_GCCAAT_L002_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 3L15A_GCCAAT_L002_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 2862506
Filtered Sequences 0
Sequence length 101
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
TGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 42557 1.4867043073446833 No Hit
TGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 41151 1.437586506368895 No Hit
TTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 32632 1.139980143273062 No Hit
TGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 25261 0.8824784996083851 No Hit
TGACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 20861 0.728767031405349 No Hit
TGGCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 15765 0.55074120368656 No Hit
GCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 15076 0.5266713851429482 No Hit
TGACTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 13488 0.47119551889148875 No Hit
TCCTCTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGT 13270 0.4635798143305202 No Hit
GACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 12625 0.44104711046893874 No Hit
TGACTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 10963 0.382986096797701 No Hit
GGGTGCATAAGGATGTTGTGTTCAGCTTGGAACACAATCATGAAGTTGAA 10201 0.3563660652589025 No Hit
TGACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 8963 0.3131172476145028 No Hit
GACCAGACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 8799 0.3073880019814806 No Hit
GACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 7448 0.26019159435823014 No Hit
AGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 7083 0.2474405293822965 No Hit
TGTCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 6787 0.23709993970318313 No Hit
GCTGCGGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 6206 0.21680303901546408 No Hit
TTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 6205 0.21676810459087245 No Hit
GACCATACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 6096 0.21296025231038818 No Hit
TGATCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 6002 0.20967641639877788 No Hit
TAGATAAGTCTAACTTATAAATTAAATTATAAGTTAAAATTAACCGTTTA 5720 0.19982490866394692 No Hit
TGACTGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 5446 0.19025287632584875 No Hit
GGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 5340 0.18654982731913924 No Hit
TGGCTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 5332 0.18627035192240643 No Hit
TGCCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 4711 0.1645760742510234 No Hit
AGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 4338 0.15154553387835693 No Hit
GCTGTAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 4242 0.14819182911756343 No Hit
CAGTAGTTTCACGGATTAAACTTGAAGTTACTAAGGAACCATGCATAGCA 4183 0.14613069806665907 No Hit
TGTCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 4175 0.14585122266992628 No Hit
TGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 4053 0.14158922286975117 No Hit
CGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 4024 0.14057612455659482 No Hit
TGGCCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 3949 0.13795604271222486 No Hit
GGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 3925 0.1371176165220265 No Hit
TGGCTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 3903 0.1363490591810113 No Hit
GGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 3879 0.13551063299081295 No Hit
TGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 3831 0.1338337806104162 No Hit
TTGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 3730 0.13030540372666466 No Hit
ACAGGAGGAGCTGCAATGAAAGCAATAATAAATACAGAAGTTGCAGTTAA 3668 0.12813946940198553 No Hit
CTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCA 3467 0.12111765005907411 No Hit
TGACGAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 3462 0.12094297793611612 No Hit
GTGTCAAACTCAAATTTAATTTCTTTCCAAACTTCACAAGCAGCAGCTAA 3306 0.11549320769982666 No Hit
GCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 3244 0.1133272733751475 No Hit
GACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 3213 0.11224430621280795 No Hit
GGGCCTTGGAAAGTTTTAGAATAAGCTGGAGGAATACGTAAATCTTCTAG 3083 0.10770283101590006 No Hit
GACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 2932 0.1024277329025686 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
TTTTT 5676915 12.358938 18.824575 7
AGCAG 1120000 5.146898 55.808205 20-24
TAGCA 1391680 4.5969176 36.15744 20-24
CACGG 759050 4.4247084 51.92281 45-49
ACTTG 1241330 4.312119 105.83546 8
GCAGA 921010 4.2324495 39.87917 25-29
CCTGC 743115 3.9496343 48.40724 15-19
CTGCG 642595 3.9393785 54.83223 15-19
GAAGT 974305 3.7120326 33.97176 60-64
AGAGA 974540 3.5305338 29.2991 90-94
AGAGC 758115 3.483875 65.1256 95-97
TCACG 831185 3.4826658 37.494946 45-49
CGGAT 716665 3.4635367 39.523766 45-49
AGTAG 897375 3.4189343 31.686275 35-39
ACGGA 730215 3.355662 40.157658 45-49
GAACC 834020 3.3228788 35.064713 70-74
CTTGA 946695 3.2886188 31.330427 55-59
GTTTC 896120 3.2737515 35.0408 40-44
GAGAG 616135 3.2658246 57.611786 95-97
GCGTT 618255 3.1422977 41.901314 15-19
AACTT 1314785 3.1216335 72.590576 7
TGCGT 613405 3.1176474 44.809334 15-19
GAGCC 526150 3.0670712 73.17922 95-97
TGAAG 788265 3.0032332 26.571198 60-64
ACCTG 715660 2.998616 29.736265 15-19
CCATG 701215 2.9380915 37.25469 75-79
GGAAC 637185 2.9281478 40.74186 70-74
GCAGC 493340 2.8758128 14.754077 20-24
CATGC 677650 2.8393543 37.063656 75-79
CTCAG 676010 2.8324826 37.414623 35-39
AGCAC 706385 2.8143592 32.212585 80-84
CTTGT 765840 2.797806 111.500175 9
TTCTC 882930 2.7965102 26.927685 30-34
AACCA 1023895 2.7881567 22.746843 70-74
ATAGC 829345 2.7394452 28.869999 80-84
AAGGA 755700 2.737727 25.16049 70-74
CACTG 647835 2.7144291 36.070824 85-89
TTCAC 894160 2.6929557 22.360458 45-49
AAGTT 978065 2.678455 25.342813 60-64
GACTC 636180 2.6655948 36.890224 25-29
AGGAA 734840 2.6621559 32.2386 70-74
GCACT 631845 2.6474311 28.127707 85-89
TTGAA 966610 2.6470857 22.899353 55-59
TGCAT 759245 2.637457 28.618734 75-79
GGATT 648580 2.598702 27.654339 50-54
CAGAC 648310 2.5829782 35.347736 25-29
AAACT 1140090 2.5738914 41.323166 6
ACCAT 882710 2.527877 26.797844 75-79
GTAGT 630145 2.5248375 27.734863 40-44
CAGTA 757740 2.5029233 29.225367 35-39
AGAAG 680985 2.4670517 13.568931 6
ATGCA 741845 2.45042 29.414518 75-79
TTTCA 974400 2.4329915 21.375582 40-44
GTTAG 606025 2.4281945 35.85552 20-24
CTGAA 720360 2.379452 28.260775 85-89
TAAGG 622855 2.3730333 31.113562 65-69
TAGAG 617235 2.3516212 32.090126 90-94
TTAGC 673705 2.3403091 31.194265 20-24
TCAGT 672880 2.337443 31.193382 35-39
AGGAG 437400 2.3184395 15.727343 90-94
GCATA 701455 2.3170059 22.64962 80-84
GAAGA 638145 2.3118522 7.2974863 50-54
CATAG 698690 2.307873 29.026077 80-84
TTGTA 796660 2.2943826 29.47787 9
GTTAC 651210 2.2621663 28.380398 60-64
AGTTT 784430 2.25916 26.111519 40-44
TCTCA 745570 2.2454448 21.157452 35-39
CATTC 744235 2.2414243 27.140625 30-34
GTAAC 671600 2.2183907 29.533003 10-14
AACCT 758200 2.171309 23.976143 10-14
TGTAA 792140 2.169295 24.866299 10-14
CTAAG 655400 2.1648796 28.936369 65-69
AGACT 653425 2.158356 29.496868 25-29
CTCAT 712635 2.146254 21.019577 30-34
TAACC 745245 2.134209 25.31542 10-14
GGAAG 401250 2.1268263 6.854129 6
AAGAG 585775 2.1221278 8.13522 50-54
GAATA 812645 2.1161256 17.832556 90-94
CGTTA 606485 2.106801 23.84328 20-24
GCTGC 339455 2.0810025 19.707455 1
GGGGG 182565 2.0715098 7.157595 1
ACTGA 624510 2.062846 28.637608 85-89
GGAGG 265230 2.0569127 13.432554 90-94
CAGCA 513355 2.0452943 10.970101 20-24
TGAAT 744125 2.0378046 22.463858 85-89
CCAAA 746155 2.031846 34.45764 4
AATAG 776190 2.0211968 22.89383 90-94
ACTCA 693115 1.9849207 23.777166 25-29
AGTTA 724460 1.9839518 24.029827 60-64
ACAGG 426435 1.9596583 10.616704 90-94
TAGTT 673880 1.9407759 25.655107 40-44
TCATT 769425 1.9211869 22.408878 30-34
CTGCA 455055 1.9066808 11.7131195 3
ATTCT 759965 1.8975661 22.477892 30-34
ACCAG 474385 1.8900312 9.434593 55-59
GTAGG 336095 1.8735063 13.762237 7
TTAAA 994260 1.8609743 17.023153 50-54
CAGAA 582905 1.8308342 9.809453 6
TAAAC 808920 1.826235 18.920769 50-54
AGGGA 341285 1.8089818 13.106794 9
TGCAG 366360 1.7705641 14.40581 4
GAGCG 260365 1.7505976 7.7556005 90-94
CCAGA 437510 1.7431148 11.557018 55-59
GATGA 457345 1.7424518 7.4365315 15-19
TCCTC 454730 1.7372183 10.015901 1
GATTA 632000 1.7307478 24.200928 50-54
CCTCC 374345 1.7249776 6.0997224 1
ATAGA 654990 1.7055924 22.775099 90-94
TTACT 680885 1.7001103 17.200974 65-69
GGAGC 252815 1.6998342 13.446557 95-97
ACCAA 623210 1.6970562 18.001776 3
AAGAA 680870 1.6858877 7.437508 45-49
TGACC 402260 1.6854697 51.29422 1
GATCG 344490 1.6648695 6.39138 95-97
CAGGC 284770 1.6600016 10.49589 65-69
ATTAA 885760 1.657893 17.007902 50-54
CAAAC 603865 1.6443781 40.225674 5
TACTA 689325 1.6366328 21.012327 65-69
GTTGT 387235 1.6317126 8.873681 35-39
ACTAA 719890 1.6252393 20.182238 65-69
TAGGG 287440 1.602287 13.70005 8
GCGTC 259965 1.5936952 5.275754 95-97
CAGAG 345825 1.5892196 9.789293 25-29
AGCTG 328225 1.5862632 5.352976 95-97
CGTCG 258690 1.5858788 5.5274997 95-97
CCATC 436280 1.5848565 7.7286577 75-79
GGCTC 258345 1.5837638 12.912997 65-69
TTTCC 495990 1.5709525 6.709557 40-44
CAGGA 339395 1.5596707 9.238681 90-94
TGTTG 369635 1.5575505 7.952149 35-39
ATCGG 322100 1.5566621 5.794434 95-97
TCTCC 405095 1.547596 5.3258705 1
TCGGA 318150 1.5375723 5.5553427 95-97
CGGAA 330730 1.5198513 5.3863297 5
GGATG 272390 1.5183933 12.549494 10-14
AGCCA 380240 1.5149413 13.98091 95-97
GAGGA 285045 1.5108815 13.143018 95-97
AGATC 455875 1.5058202 5.283151 95-97
GACCA 376170 1.4987258 23.732817 2
AACAG 465780 1.4629587 9.966094 60-64
GAGAT 383170 1.4598503 9.851866 25-29
AGAAA 589570 1.4598217 5.227769 60-64
GAGCT 299280 1.4463763 6.4609184 95-97
TCCAA 503350 1.4414778 5.5547667 3
GAGAA 390230 1.4137133 9.568221 50-54
GCCAA 352535 1.4045599 17.021658 3
CATCA 487885 1.3971895 5.587461 80-84
CAACA 512675 1.3960595 7.2221823 8
GAAAC 444375 1.3957282 6.6938224 60-64
AGCGT 286945 1.3867631 5.2909665 90-94
GGCCA 237415 1.3839564 20.481249 2
GATGT 344700 1.3811289 10.198557 30-34
AAAGA 556320 1.3774922 5.8756986 45-49
TGATG 341975 1.3702105 8.753055 30-34
TGCTG 267550 1.3598301 5.188953 1
GCTCC 254160 1.350853 5.6506386 1
GCTGG 184470 1.3043845 5.7024984 1
TTCCG 295330 1.3013611 9.019593 40-44
TTGTT 429230 1.3000468 6.875878 35-39
TACAG 390175 1.2888038 7.2389154 90-94
GTAAA 491230 1.2791617 5.520027 45-49
TAAAG 490260 1.2766359 6.141868 45-49
AGGCT 264050 1.2761148 9.734961 65-69
CTCTT 399660 1.2658457 6.870367 3
GCTCA 301725 1.264228 10.711911 65-69
ATACC 440790 1.2623204 5.86945 75-79
GGGAT 225545 1.2572635 13.917026 10-14
TCTTG 344000 1.2567185 6.8219786 4
TACCA 436275 1.2493905 6.2508755 75-79
AAGTA 479750 1.2492677 14.3014555 8
ATGAT 453150 1.2409625 6.0396 15-19
TGTTT 409700 1.2408946 7.9917984 35-39
CTACA 430870 1.2339118 6.823813 85-89
AAACA 554545 1.1904517 5.1576796 60-64
CCCAA 344020 1.1883166 8.268616 3
CTCAC 325290 1.1816677 7.3480287 65-69
CCAAC 341705 1.1803203 5.1278872 5
TGGCC 191940 1.1766732 32.0644 1
TCCGT 266325 1.1735516 9.312766 40-44
AGATG 305690 1.164657 7.5920296 25-29
GCCCA 229340 1.1590565 7.4710374 2
CCTCT 302685 1.1563563 7.869813 2
AGAAC 355915 1.1178861 7.2721443 50-54
CCGAA 271775 1.0827981 33.37627 4
TCAAC 375210 1.0745144 7.739753 7
GATAC 322340 1.064735 5.7635126 75-79
CAATA 465065 1.0499408 5.679232 80-84
GTCCA 244535 1.0246018 5.6959467 2
ATGTT 355190 1.022948 5.8027973 30-34
CTTTT 387965 1.0187602 5.22966 6
CTCCG 189355 1.0064163 5.7967005 2
TTGAC 287110 0.9973596 6.999146 6
TCTAC 330930 0.9966671 7.13252 85-89
CCGTA 231140 0.9684768 10.431203 40-44
CGAAC 242100 0.96456796 31.747671 5
CGTAA 291950 0.9643525 6.512604 40-44
TCAAA 426320 0.962469 5.1950183 4
AACAA 447030 0.9596473 5.9070063 9
GATAG 250310 0.9536632 9.58501 15-19
GAACT 288260 0.95216393 28.415686 6
GGGTG 115470 0.94175625 12.577013 1
GGTGC 132675 0.93814284 10.102964 2
GTGCA 190800 0.92210835 7.5499597 3
TGATA 334430 0.9158449 6.15116 15-19
GGGGT 111990 0.91337395 5.4020214 1
CTAAA 402900 0.9095955 6.4283285 4
TCCCC 195220 0.8995716 5.469179 1
CCCCC 161650 0.898458 5.2456093 1
GGCTG 126410 0.8938431 6.495084 1
GGATA 234035 0.89165664 7.2955775 70-74
TGTCC 202245 0.8911853 8.542033 1
GACCT 211810 0.8874841 10.092924 2
CCTAA 306825 0.8786757 9.689529 4
GGCCT 143270 0.87830555 6.6741643 2
GGGCC 102205 0.8716913 10.933964 1
TGCCC 161600 0.85889924 8.139396 1
ACCTA 296375 0.8487493 7.070587 3
GGGGC 85800 0.8440483 5.107614 1
GTCAA 254850 0.84180593 8.560273 6
ACCGA 207705 0.82753235 20.98423 3
CCGTC 154665 0.82203996 5.8405266 4
CTGTC 182520 0.8042678 9.124257 4
TGTCA 224035 0.77825034 7.858458 5
TGGCT 152595 0.7755682 6.96464 1
GACCG 127315 0.7421537 29.890408 2
GGCCG 85415 0.7284917 18.363754 2
GCCGA 116630 0.6798679 13.563713 3
GCCCC 105625 0.6771397 7.3507147 1
TCCGA 159385 0.66782326 5.1288095 3
GGCCC 88120 0.65159166 7.886476 1
AGTCT 184390 0.64053196 5.1087046 7
TGCGG 89620 0.6337016 7.4271626 3
GACTG 128785 0.622399 9.109159 2
CCCCG 96810 0.62062854 6.771013 2
GTCCC 114670 0.6094677 5.111623 1
TGACT 170680 0.5929063 12.458362 1
GACTT 165920 0.5763711 8.121301 6
CTAAC 200200 0.57332635 8.775026 5
GACCC 113170 0.57194746 6.0930514 2
GCCCG 76855 0.5682941 6.2923703 2
CCCGA 111820 0.56512463 6.333362 3
GCGGA 81955 0.551035 6.5468745 4
TAACT 227315 0.53970355 7.64203 6
GTCCG 86615 0.5309865 5.891943 2
GGACC 88375 0.5151619 5.2130737 1
CCCGT 92765 0.4930432 5.515651 3
GTCTA 136740 0.47500592 5.080061 8
GACTA 119080 0.39333823 5.791608 2
Produced by FastQC (version 0.10.1)