FastQCFastQC Report
Thu 31 Jul 2014
3L15A_GCCAAT_L002_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 3L15A_GCCAAT_L002_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 2862506
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[OK] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 32101 1.121429963814923 No Hit
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 28684 1.0020590349854288 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 24864 0.8686095330455202 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 18698 0.6532038710137201 No Hit
ATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCA 18480 0.6455881664527515 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 17167 0.5997192669639819 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 16562 0.5785839400860644 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 16069 0.5613612687624061 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 15659 0.5470381546798505 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 13866 0.4844007313871132 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 13306 0.46483745361581774 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 12562 0.438846241719668 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 11974 0.4183048000598077 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 11948 0.4173965050204262 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 10305 0.35999924541642886 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 10043 0.35084642617342987 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 9783 0.3417634757796141 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 9583 0.33477659086129424 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 7274 0.2541130044792919 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 7132 0.24915231618728484 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 7123 0.24883790636596048 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 7038 0.2458684802756745 No Hit
GTATGGGCTTGGATGTTCTTATTTGGACATCTTGTTTGGGCTACTGGGTT 6429 0.22459341569939067 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 6333 0.22123971093859718 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 6281 0.219423120859834 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 6246 0.218200415999128 No Hit
GCTAAAAACTATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGA 6218 0.21722225211056329 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 6125 0.21397335062354456 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 5942 0.2075803509232819 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 5802 0.20268953148045804 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 5656 0.19758910549008457 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 5442 0.19011313862748236 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 5328 0.18613061422404006 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 5320 0.18585113882730725 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 5057 0.1766633851597167 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 4942 0.1726459263316828 No Hit
CACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTAT 4667 0.16303895956899303 No Hit
GCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATG 4552 0.15902150074095914 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 4405 0.15388614032599407 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 4370 0.1526634354652881 No Hit
CTGCTGCTATCATCCCTACTTCTGCAGCTATCGGTTTGCACTTCTACCCA 4362 0.1523839600685553 No Hit
AGCTCCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTC 4227 0.14766781274868945 No Hit
TTCATGCGTTGGAGAGACCGTTTCTTATTCTGTGCAGAAGCTATTTACAA 4146 0.1448381243567699 No Hit
GCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGT 3731 0.13034033815125629 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 3596 0.1256241908313904 No Hit
TATGCACCCATTCCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTC 3498 0.12220061722141369 No Hit
GCTCATGGTAAGGCTTTATATGGTTTTGATGTTCTTTTATCATCTGCAGA 3352 0.11710019123104022 No Hit
ACCCAATTTGGGAAGCTGCTTCCGTTGATGAATGGCTTTACAACGGTGGT 3202 0.11186002754230034 No Hit
ATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAA 3200 0.11179015869311716 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 3191 0.11147574887179275 No Hit
GATGAAATTAGCAGTATTATCCGTAAACAGATAGAAGACTATAGTCAAGA 3180 0.11109147020128517 No Hit
ATGGTAGAGCTGTATACGAATGTCTTCGTGGTGGACTTGATTTCACTAAA 3134 0.10948448667007162 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 3065 0.10707401137325127 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 3038 0.10613078190927809 No Hit
GTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCC 3028 0.10578143766336211 No Hit
TACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTAT 3005 0.10497794589775533 No Hit
GTAGGTGGTGTAGCTACTGAAATTAATGCAGTAAATTATGTGTCTCCACG 2923 0.10211332308124418 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AAAAA 2464430 7.623253 11.682232 90-94
ACTTC 921280 3.7098675 8.057389 65-69
CTGCT 830590 3.5169702 11.214616 4
CTACT 790615 3.1836972 9.780962 85-89
AGCTG 797430 3.1118484 9.84362 3
AACTT 1008900 3.1108835 6.125245 60-64
CTTCA 758415 3.0540323 8.819103 65-69
TGCTA 930565 3.0171552 5.258991 95-97
TCAAG 791875 2.938781 6.1153145 65-69
TCTCT 816365 2.8720465 8.300069 20-24
TTCAA 915995 2.8244164 6.9076552 55-59
CTCTC 526905 2.7709587 10.427749 30-34
TGGTG 1003950 2.7558942 13.713185 8
CTGGT 798530 2.7224348 8.924836 1
CCGTG 531090 2.706606 7.2362866 80-84
GAAGA 774710 2.6496809 5.125887 95-97
GGTGG 780810 2.5797093 8.563146 7
GCTGC 500390 2.5501487 12.045152 3
GGTGT 920940 2.5280275 17.707653 9
GGTTC 738555 2.5179613 7.1671634 4
GAAAC 592160 2.5154126 6.5744443 80-84
TCCTT 712860 2.5079064 7.6223197 40-44
CGGTG 607535 2.4929435 15.526279 9
CTTCT 701295 2.4672196 5.7517343 40-44
TATTC 913905 2.461938 9.55173 5
GCTGG 599250 2.458947 11.140339 4
ATGCA 660705 2.4519873 6.8744173 45-49
GGCTC 480455 2.4485538 7.4654884 30-34
CATGG 626000 2.4428692 9.2204075 2
GAGAA 707380 2.4193974 6.1400123 90-94
GTTCC 569180 2.410081 6.1143746 55-59
CGTGA 617225 2.408626 7.046118 9
ATCCG 494650 2.3973954 7.358218 75-79
TATGC 737610 2.3915405 7.1636333 45-49
CAACT 511890 2.3594077 6.405482 9
TGGCT 676115 2.3050842 7.430025 9
CAAGT 619105 2.2976024 6.502926 70-74
CCTTA 570480 2.297244 6.47727 45-49
TCCGT 537585 2.276298 5.946491 75-79
TCTGC 529285 2.2411532 7.332049 85-89
TGAAA 787535 2.2379448 5.251252 80-84
CTCTA 548555 2.208955 6.815792 25-29
GCTCT 521450 2.2079775 7.103398 30-34
ATGGT 845215 2.2064922 6.7895913 2
TCAAC 478135 2.203824 5.726282 8
TTCCT 618715 2.1766958 6.0445056 55-59
ACCCA 315290 2.172337 26.296473 1
GTGGC 527815 2.1658225 8.411561 8
TGGTT 948280 2.162779 5.6558485 3
GCAGC 369125 2.1532278 6.0007033 1
CTTGG 622745 2.1231294 13.483446 6
GCATG 534355 2.0852385 5.9967866 50-54
AAACT 590590 2.0844014 5.438148 85-89
TACTG 638720 2.0709112 5.9799533 85-89
ACTGA 557745 2.0698853 5.4167323 90-94
AATCC 447645 2.0632894 8.761916 75-79
ACTAC 447505 2.062644 8.829591 85-89
TAGCT 628635 2.0382128 7.62624 2
CTCCT 383570 2.0171697 7.052536 70-74
GTGAA 672825 2.010467 6.324666 80-84
GTGCT 585445 1.9959623 5.697954 40-44
CAGTG 509890 1.9897678 6.437139 40-44
CTATG 607470 1.9695898 6.20219 45-49
ACAAC 373300 1.9694457 5.0875216 85-89
TTCGG 572025 1.9502094 12.875295 7
CTGAG 499730 1.9501197 6.345161 90-94
CAGCT 399100 1.934298 5.2934837 2
CAGCA 348645 1.9341259 5.437774 2
GTGTA 734300 1.9169407 12.640155 2
CGGAA 428150 1.9124118 5.477944 95-97
AGTGC 486995 1.9004233 7.673214 40-44
TCGGT 556560 1.8974845 13.698283 8
TTCAG 583650 1.8923584 5.191432 35-39
CTATT 698535 1.8817599 5.046853 35-39
GCTCC 292465 1.8511711 5.914425 70-74
ATTCA 591300 1.823239 5.1674986 35-39
GCTTG 523380 1.7843635 12.499983 5
GCTAC 366590 1.7767333 8.289158 1
TGCTT 626805 1.7755127 10.527093 4
GTAAC 477505 1.7721013 5.8995075 60-64
GAATG 591090 1.7662346 6.6691036 95-97
CTCTG 413840 1.7523242 5.294949 20-24
GCAGG 372055 1.7474626 5.888349 90-94
AATGA 613600 1.7436724 5.545621 95-97
GTAGC 437700 1.7080572 8.257612 10-14
CATGC 352020 1.7061177 16.211733 2
CCACA 247165 1.7029582 14.539381 1
GATCG 432830 1.6890527 5.298538 95-97
TGTAG 646555 1.6878763 9.527308 9
TTGGT 739260 1.686059 14.99882 7
GCACA 303245 1.6822668 5.1889243 95-97
TGAGA 562060 1.6794903 6.100568 90-94
CCTCC 212605 1.6713289 5.049456 1
CACCC 184085 1.6564049 5.1696715 50-54
AACTA 466215 1.645438 5.662669 85-89
CTTTC 467450 1.6445316 5.600257 7
CGCAG 281540 1.6423157 7.2894726 90-94
CCAGC 225940 1.6369123 6.184889 1
CACAC 235705 1.6239992 6.248593 95-97
CCTGT 381140 1.6138624 8.233318 1
GATGG 510140 1.6028742 6.7964144 1
AGATC 428865 1.5915898 5.232672 95-97
GCTTC 375645 1.590595 5.136132 15-19
CATCC 263920 1.5886554 5.0419545 9
CCCAA 230020 1.5848298 25.120676 2
CCAAT 341620 1.5745978 16.921675 3
GTCTG 458340 1.5626222 6.4781466 90-94
CACAA 295085 1.5568012 5.122225 4
ATTCG 477515 1.5482389 11.420993 6
GTATT 702040 1.5227274 8.164871 4
ATGCT 467965 1.5172751 11.262101 3
AGTTT 697235 1.5123051 5.0169153 70-74
AGTAA 521030 1.4806153 5.6353865 60-64
AACGC 259995 1.4423354 7.179147 95-97
TACTT 535355 1.4421748 5.102961 3
CTGTA 443285 1.4372555 6.18277 8
ATGAG 477000 1.4253227 6.421868 95-97
TGTAT 642680 1.3939751 7.9482903 3
ACGCA 249450 1.3838365 5.629379 90-94
GCAAC 247230 1.3715209 6.150461 1
TGGTA 524510 1.3692695 5.8480334 2
ACATG 365970 1.3581761 16.457998 1
GGCTG 325485 1.3355869 5.746276 9
CAGGT 337630 1.3175495 6.324571 95-97
ATGGC 335150 1.3078716 5.221258 1
ATGCC 268820 1.3028765 6.383062 9
ACGGT 324545 1.2664872 5.831645 4
ACTTT 463550 1.2487417 5.1995883 4
CTAAC 267265 1.2318802 5.490354 95-97
GCCTG 238720 1.2165941 6.761288 6
GCGGC 198210 1.2157867 7.397414 7
CACAT 260550 1.2009293 9.663872 2
TGGCC 231750 1.1810727 8.54323 1
GGTAC 298520 1.1649286 6.6166377 3
GACGG 243190 1.1422113 7.053 3
AAGCC 204205 1.1328375 6.8201003 1
TGGTC 329410 1.1230601 10.9963045 8
GGTCA 286870 1.1194662 12.336854 9
CAATT 349395 1.0773389 11.596665 4
CTGAC 220485 1.0686136 6.956972 1
GTACT 329020 1.066776 5.4738903 4
ATGGG 339340 1.0662159 8.158746 3
TGACG 268880 1.0492631 5.8039002 2
TCGTG 303220 1.0337703 5.9129515 8
TGCGG 242430 0.9947811 5.254988 6
TGGGT 359010 0.9855009 5.2132263 4
ATTGG 375560 0.9804253 9.0034275 6
AAGGC 215080 0.9606951 5.684013 1
GGTCG 233415 0.9577891 6.860126 6
GCCCA 129775 0.94020665 5.134739 2
TGGGC 224105 0.91958666 5.0062904 4
AATTG 351655 0.8730451 8.156674 5
CGGCT 168825 0.86038655 5.839855 8
GGCCT 164980 0.84079134 6.4425263 5
GTTCG 244980 0.83521223 6.896143 5
AGGCC 142450 0.83095783 5.9073634 2
GGCCC 108255 0.82469916 6.270837 1
GGGTC 198725 0.81544304 6.6909657 5
CGGTA 208910 0.8152392 5.7219343 5
CCACG 94910 0.68761337 5.145294 1
GTCGT 197405 0.67301446 5.605571 7
Produced by FastQC (version 0.10.1)