FastQCFastQC Report
Wed 6 Aug 2014
20S15C_CTTGTA_L004_R2_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 20S15C_CTTGTA_L004_R2_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 14500133
Filtered Sequences 0
Sequence length 101
%GC 43

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[FAIL] Per base sequence content

Per base sequence content

[FAIL] Per base GC content

Per base GC content graph

[FAIL] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
TGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 262184 1.8081489321511741 No Hit
TGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 234028 1.6139714028829941 No Hit
GCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 128824 0.8884332302331296 No Hit
TGACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 124230 0.8567507622171465 No Hit
TGGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 102579 0.7074348904247981 No Hit
GCTGCGGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 78113 0.5387054035987118 No Hit
CAGTAGTTTCACGGATTAAACTTGAAGTTACTAAGGAACCATGCATAGCA 77305 0.5331330409176247 No Hit
TGACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 76173 0.5253262159733293 No Hit
TGGCCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 69720 0.4808231758977659 No Hit
AGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 61102 0.4213892382918143 No Hit
GACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 56640 0.39061710675343464 No Hit
GGGTGCATAAGGATGTTGTGTTCAGCTTGGAACACAATCATGAAGTTGAA 52168 0.359776010330388 No Hit
TGACTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 50442 0.3478726712368776 No Hit
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 47341 0.32648666050166575 No Hit
ACAGGAGGAGCTGCAATGAAAGCAATAATAAATACAGAAGTTGCAGTTAA 42589 0.2937145473079454 No Hit
GCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 37856 0.26107346739509213 No Hit
TGACTGTCAACAACAGATTGGTTGAAGTTGAAACCGTTTAAGTTGAAAGC 37230 0.25675626561494297 No Hit
GACCAGACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 36517 0.25183906933819156 No Hit
GCTGTAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 36067 0.24873564952818017 No Hit
GACCATACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 31909 0.22006005048367488 No Hit
TGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 30623 0.21119116631550897 No Hit
TGACTGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 30093 0.2075360274281622 No Hit
TCCTCTTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGT 29517 0.20356365007134763 No Hit
GCTGCTGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 29031 0.2002119566765353 No Hit
TGGCCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 28492 0.19649474939298833 No Hit
GGCTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAA 28453 0.19622578634278734 No Hit
AGCGTATTGGAAAATTAATCTACCAAAGTAACCGTGAGCAGCTACGATGT 26432 0.18228798315160283 No Hit
TAGATAAGTCTAACTTATAAATTAAATTATAAGTTAAAATTAACCGTTTA 26032 0.17952938776492602 No Hit
GACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 24790 0.17096394908929458 No Hit
TTGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCAC 24561 0.1693846532304221 No Hit
GCTGCCGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 24531 0.16917775857642134 No Hit
TGATCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 20387 0.14059871037044971 No Hit
GCAGAGGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 20118 0.13874355497290955 No Hit
GTTGCAGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 19695 0.13582634035149885 No Hit
ACAGGGGGAGCTGCAATGAAAGCAATAATAAATACAGAAGTTGCAGTTAA 19007 0.13108155628641477 No Hit
GCAGATGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 17962 0.12387472583872162 No Hit
GCTGTGGAAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAG 17121 0.11807477903823364 No Hit
GCAGTAGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 17071 0.11772995461489906 No Hit
GGCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 16988 0.11715754607216362 No Hit
TGTCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 16916 0.1166609989025618 No Hit
GACCTAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 16101 0.1110403608022078 No Hit
CGTCCCATATTTAAGTCGTCTGCAAAGGATTCATCTCCCCGATCGTCTGG 15884 0.10954382280493566 No Hit
GACCCAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACGG 15487 0.10680591688365894 No Hit
TGGCTAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 15479 0.1067507449759254 No Hit
GCAGACGTAGGGATGATAGCAGCAGAGATGATGTTGTTTCCGTAAAGAAG 15069 0.10392318470458167 No Hit
TGCCCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 15029 0.10364732516591399 No Hit

[FAIL] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
AGCAG 6292610 4.877007 54.269146 20-24
TTTTT 8040110 4.635617 8.264664 7
TAGCA 7041775 4.4132752 35.01947 20-24
CACGG 4077940 4.259505 42.03482 45-49
ACTTG 5826685 4.2108436 103.76144 8
GCAGA 5045200 3.910218 35.61282 25-29
GTTTC 4683985 3.903298 37.51998 40-44
CCTGC 3392675 3.8620017 46.7129 15-19
TCACG 4289095 3.6227655 34.05077 45-49
CTGCG 2996795 3.6094742 48.733505 15-19
CGGAT 3861025 3.450594 33.58382 45-49
GAAGT 5187075 3.4396803 30.778337 7
GAACC 4572530 3.3493645 29.600658 70-74
AGAGA 5725315 3.2925038 21.418274 90-94
CTTGA 4469600 3.2301018 29.733995 55-59
AACTT 6312820 3.1993268 72.91169 7
AGTAG 4772210 3.164573 30.684067 9
AGAGC 4029480 3.122997 50.02941 95-97
ACGGA 3957670 3.0673418 30.886871 45-49
GCAGC 2926840 3.0571532 18.79068 20-24
TTCTC 3876750 3.053285 30.64424 30-34
CTTGT 3603185 3.0026367 119.791214 9
GCGTT 2877250 2.9650872 39.130466 15-19
TGCGT 2811615 2.8974485 41.39945 15-19
CCATG 3426445 2.8941321 34.47234 75-79
AACCA 5503710 2.8271513 19.734108 70-74
CATGC 3312850 2.7981844 33.895027 75-79
ACCAT 4705540 2.7872229 31.050236 75-79
ATAGC 4440165 2.7827744 24.707386 80-84
ACCTG 3285905 2.7754254 27.607855 15-19
TTCAC 4037100 2.7574043 23.116629 45-49
TGCAT 3769850 2.7244048 27.604336 75-79
GAGCC 2574125 2.6887343 59.246902 95-97
TTTCA 4537540 2.6516995 22.88483 40-44
AAGTT 4914280 2.635189 22.337002 60-64
CACTG 3086350 2.6068723 32.6227 85-89
GCTGC 2161360 2.6032388 37.67162 1
TGAAG 3907570 2.5912082 21.328823 60-64
CTCAG 3021855 2.5523968 33.997623 35-39
TTGAA 4715330 2.5285063 20.692715 55-59
GAGAG 3080580 2.5262234 40.70532 95-97
AGCAC 3439405 2.5193539 27.170633 80-84
GCACT 2982510 2.5191643 25.787756 85-89
AGGAG 3042845 2.4952788 19.612736 90-94
AGAAG 4311435 2.4794126 16.320034 6
TTGTA 3953100 2.4443219 27.58676 9
GACTC 2875800 2.4290318 33.854935 25-29
GGATT 3166600 2.4213493 24.34279 50-54
GGAAC 3110985 2.4111292 31.118326 70-74
GTAGG 2503450 2.3672647 25.412231 7
GAAGA 4097415 2.3563342 8.990797 50-54
ATGCA 3708965 2.324511 25.598804 75-79
AAACT 5250135 2.307475 31.86108 6
GTAGT 3014550 2.3050838 24.310198 40-44
ACAGG 2971210 2.3027985 13.595346 60-64
CATTC 3365475 2.2986734 27.894772 30-34
GTTGT 2598470 2.2911346 14.359281 35-39
CTGAA 3654235 2.2902102 24.292645 85-89
TTAGC 3155770 2.2806199 29.46014 20-24
AGTTT 3683540 2.2776444 25.325142 40-44
GTTAC 3141435 2.2702603 27.176767 60-64
AGGAA 3899110 2.2422931 23.278425 70-74
TCTCA 3275105 2.2369492 21.995909 35-39
TCAGT 3076610 2.2234125 29.390894 35-39
AAGAG 3857010 2.2180827 9.77851 50-54
AAGGA 3838750 2.2075815 18.459976 70-74
CAGTA 3505610 2.1970627 25.273855 35-39
CTCAT 3196375 2.1831753 21.89327 30-34
CAGAC 2968205 2.1742013 29.354345 25-29
GTTAG 2843070 2.1739612 31.03808 20-24
GCATA 3463615 2.1707432 19.869404 80-84
TGTTG 2458760 2.167949 13.144253 35-39
TGTAA 4027630 2.1597397 22.143564 10-14
ACCAG 2943790 2.1563177 12.638118 55-59
GATGA 3235950 2.1458402 10.130142 15-19
GGAAG 2615810 2.1450896 11.435382 6
TCATT 3604190 2.1062577 23.721115 30-34
CATAG 3351615 2.10055 24.968616 80-84
TAACC 3520730 2.085427 23.80379 10-14
CTGCA 2460475 2.0782297 16.806623 3
TAGAG 3130525 2.07593 25.230305 90-94
CAGCA 2821045 2.0664072 16.168467 20-24
AACCT 3462750 2.0510836 22.741962 10-14
GGCTC 1700105 2.0476828 19.01415 65-69
TTTCC 2593100 2.0422964 12.2025585 40-44
CGTTA 2819835 2.0378456 22.872837 20-24
GGAGG 1727875 2.0205235 16.671608 90-94
TAAGG 3038800 2.0151052 24.912613 65-69
ATTCT 3447960 2.0149581 23.938131 30-34
CTAAG 3210610 2.0121782 25.002165 65-69
GTAAC 3199140 2.0049896 25.394575 10-14
CCATC 2465520 1.9681872 14.14792 75-79
TAGTT 3179735 1.9661266 24.879055 40-44
AGGGA 2396355 1.9651259 22.162424 9
TAGGG 2077585 1.9645665 25.337618 8
AGTTA 3649910 1.9571947 21.41094 60-64
CAGAA 3599530 1.9563925 12.30378 5
TGAAT 3642840 1.9534036 19.88073 85-89
TTCCG 2000835 1.9487426 15.187678 40-44
CCAGA 2627945 1.924962 16.810253 55-59
GATCG 2148255 1.9198931 6.7879686 95-97
CAGGC 1836895 1.9186801 14.758701 65-69
ACTGA 3051875 1.9126947 24.540346 85-89
GGAGC 1725380 1.9068638 17.628687 95-97
GCGTC 1571575 1.8928753 6.6735425 95-97
AGACT 3003060 1.8821008 25.331861 25-29
TTAAA 4987480 1.8755158 15.607998 50-54
TTACT 3209340 1.8755107 18.5413 65-69
GAATA 4020075 1.8694652 14.522304 90-94
ACTCA 3144015 1.862288 22.57853 25-29
TGCAG 2080925 1.8597205 18.719381 3
TTGTT 2589495 1.8463087 12.039989 35-39
CGTCG 1522220 1.8334299 6.887976 95-97
GAGCG 1655725 1.8298821 7.942064 90-94
GATGT 2379345 1.8193725 16.231998 30-34
AATAG 3872690 1.8009264 18.387486 90-94
AGCTG 2009590 1.7959684 9.045853 9
ATGAT 3348810 1.7957356 9.093025 15-19
GGATG 1877900 1.7757441 17.640089 10-14
ATCGG 1986200 1.775065 6.1675706 95-97
ATTAA 4674655 1.7578796 15.565901 50-54
TGACC 2077800 1.7550048 63.60734 1
TCGGA 1963495 1.7547734 5.925798 95-97
CCAAA 3373515 1.7329108 28.878378 4
TGATG 2263625 1.7308869 12.556703 30-34
TGTTT 2407580 1.7166034 15.438712 35-39
AGATC 2712340 1.6998985 5.8795686 95-97
TCCGT 1743290 1.6979029 15.537117 40-44
GAGAT 2540685 1.6847923 14.161816 25-29
TCCTC 1826235 1.6810598 7.330527 1
TACTA 3312140 1.6785871 20.322905 65-69
CAGAG 2161895 1.6755491 12.3939705 25-29
ACCAA 3253135 1.6710738 18.243835 3
GTCGT 1612445 1.66167 6.0491905 95-97
TAAAC 3779135 1.6609592 16.933075 50-54
CCTCC 1540465 1.657316 8.1309 1
CGGAA 2120970 1.6438307 9.64618 5
GATTA 3060745 1.6412662 21.418669 50-54
AGCGT 1828240 1.6338962 6.1118975 90-94
AAGAA 3989235 1.608808 9.479816 45-49
CAGGA 2071575 1.6055478 11.057093 90-94
AGGCT 1775800 1.5870306 13.868993 65-69
GCTCA 1871090 1.5804081 17.650478 65-69
CTACA 2660865 1.5761048 10.289275 85-89
ATAGA 3380390 1.5719911 18.293695 90-94
GAGCT 1739585 1.5546653 9.523765 95-97
CATCA 2611475 1.5468497 8.319129 80-84
CCACC 1646535 1.5362285 6.042625 1
CTCAC 1908325 1.523387 13.525022 65-69
ACTAA 3457020 1.5193871 17.835543 65-69
TACCA 2555730 1.5138303 9.628589 75-79
GAGGA 1831870 1.5022212 15.552379 95-97
TACAG 2392490 1.4994397 9.105576 90-94
GGGAT 1583110 1.4969903 19.761929 10-14
GGGGG 894935 1.4922975 5.723676 1
ATACC 2464100 1.4595555 9.296076 75-79
TCGTG 1415405 1.4586148 5.316476 95-97
CTGCT 1497385 1.4584001 6.4490523 2
ATGTT 2344405 1.4496167 10.763941 30-34
AAAGA 3589810 1.4477249 7.1299615 45-49
TAAAG 3101675 1.4423796 8.34231 45-49
AACAG 2645350 1.437783 13.243904 60-64
TGCTG 1391050 1.4335164 6.062432 1
GTAAA 3078600 1.4316489 7.611546 45-49
CTCTT 1802675 1.4197667 5.0725017 3
TCTAC 2066775 1.4116404 12.932656 85-89
GAAAC 2595205 1.4105287 8.693067 60-64
GAGAA 2426480 1.3954158 12.49461 50-54
GACCA 1846510 1.3525633 23.876484 2
AGAAA 3343890 1.3485485 6.3492904 60-64
AGCCA 1827960 1.3389754 10.835429 95-97
CCGTA 1583660 1.3376316 17.530853 40-44
GCTCC 1155415 1.3152498 6.210779 1
AGATG 1982060 1.314354 11.425193 25-29
CAAAC 2522730 1.2958786 30.934715 5
AAGTA 2777635 1.2916903 22.404928 8
AGAAC 2281455 1.2400013 10.417976 50-54
GATAC 1961715 1.2294611 8.476861 75-79
GTGCT 1177930 1.2138902 10.40183 9
AAACA 3181405 1.212598 6.5805383 60-64
CAATA 2730320 1.1999968 8.6236 80-84
TTGGT 1345985 1.186788 9.842349 7
GCTGG 929475 1.1845169 5.9919543 1
TCAAT 2332220 1.1819654 7.5714483 80-84
CCTCT 1272120 1.1709938 6.0910587 2
GCCAA 1596560 1.1694754 12.029926 3
CCGAA 1593115 1.166952 32.19388 4
GGCCA 1109180 1.1585647 14.889805 2
CGTAA 1836030 1.1506908 10.661406 40-44
ATCAA 2595500 1.1407424 6.7545834 80-84
CCCAA 1645470 1.1391455 9.937236 4
GATAG 1710780 1.1344614 13.938054 15-19
TGATA 2107585 1.1301523 8.863354 15-19
CCTTT 1430735 1.1268308 5.2885404 4
TATCT 1879845 1.0985653 8.32716 85-89
GCCCA 1104805 1.0906545 6.1339607 2
CCAAC 1566845 1.084714 7.877005 5
ATCTA 2132765 1.0808817 7.5672426 85-89
TCAAC 1803615 1.0683316 5.853652 7
TGGCC 880600 1.0606341 30.150265 1
GGATA 1589125 1.0537889 10.8634815 70-74
GTTGC 1016150 1.0471712 5.698555 1
CAACT 1730950 1.0252901 6.8602543 6
CCAAT 1692270 1.0023788 5.0850215 3
AATAT 2660855 1.0006006 6.0455976 80-84
TGGTC 948880 0.9778477 10.826027 8
GGGTG 714355 0.96323955 10.798958 1
CCATA 1625755 0.96298015 5.0608892 3
GGTGC 753395 0.96012175 9.557707 2
ACCGA 1299420 0.9518214 22.07778 3
ACCCA 1360240 0.94168305 7.0723987 3
TGTGC 908045 0.935766 10.265835 8
ATTGG 1223310 0.9354073 9.185717 6
GTGCA 1039640 0.92912525 7.237041 3
CTCCG 815020 0.92776614 6.539758 2
ATATC 1822620 0.9237009 6.615654 85-89
CCTAA 1533885 0.9085629 12.110005 4
GAACT 1446595 0.9066211 27.916615 6
CTGCC 795600 0.9056597 5.9241543 2
CCGCC 674045 0.8967804 5.403711 1
GGCTG 701550 0.8940508 7.1991224 1
CGAAC 1220060 0.8936903 30.070297 5
CCCAT 1100595 0.87858826 5.2368555 4
GCTGT 849840 0.8757841 8.044754 1
ACCTA 1478285 0.87562954 8.828689 3
TGTCC 891945 0.868723 7.0849786 1
GACCT 1026575 0.86709213 12.245356 2
CCGTT 882835 0.85985005 6.3917007 4
TCCCC 789985 0.8499089 5.683073 1
GGTGT 778630 0.84900004 8.010702 6
CCCAG 841330 0.8305541 5.3630857 2
TGCCC 724635 0.8248777 7.580033 1
GCAGG 731620 0.80857533 6.3585014 1
CTGTC 823510 0.80206966 7.037438 4
CGTCC 683620 0.7781888 5.8099413 1
GGCCT 644410 0.7761562 7.201714 2
CCGTC 681825 0.77614546 6.4644766 4
GTGTG 704815 0.7685139 10.913596 7
CCCCC 610405 0.76753575 6.8074503 1
TGTCA 1046170 0.7560488 5.498803 5
GTCAA 1199065 0.7514872 5.926974 6
CGCCC 558105 0.7425285 5.24884 1
TGCGG 544215 0.6935441 14.960566 3
GGGCC 457235 0.6810359 8.088987 1
AGTCT 939460 0.67893136 5.488871 7
GACCG 646590 0.67537856 30.233534 2
GGTCA 749850 0.67014015 9.178032 9
CCCCG 478365 0.6364387 7.548579 2
CAGGG 575250 0.63575757 6.1016607 2
TAACT 1239335 0.628093 9.960858 6
GGCCG 420470 0.6262757 15.763103 2
GGTAG 657275 0.62151986 5.803519 6
CTAAC 1043005 0.617801 11.014439 5
GCCGT 497295 0.59896433 5.227212 3
GCCGA 569210 0.59455323 11.289849 3
GACCC 602120 0.594408 9.368762 2
GTCCC 520890 0.59294754 5.2770796 2
TGACT 819045 0.5919095 10.878305 1
GCCCC 444895 0.5919087 6.281959 1
GACTG 660315 0.5901228 6.992554 2
TCCCG 507120 0.57727265 5.1357393 2
GACTT 795705 0.5750421 7.9799733 6
GGCCC 401795 0.5656117 6.6146965 1
GCGGA 502210 0.5550349 11.951563 4
CCCGA 547810 0.5407936 6.2646875 3
GTCTA 717255 0.5183477 5.4981093 8
GCCCG 357810 0.5036935 7.033147 2
CCCGT 437610 0.49814692 6.873247 3
GTCCG 387735 0.4670054 5.4982595 2
Produced by FastQC (version 0.10.1)