FastQCFastQC Report
Wed 6 Aug 2014
20S15C_CTTGTA_L004_R1_001.fastq.gz

Summary

[OK] Basic Statistics

Measure Value
Filename 20S15C_CTTGTA_L004_R1_001.fastq.gz
File type Conventional base calls
Encoding Sanger / Illumina 1.9
Total Sequences 14500133
Filtered Sequences 0
Sequence length 101
%GC 44

[OK] Per base sequence quality

Per base quality graph

[OK] Per sequence quality scores

Per Sequence quality graph

[WARN] Per base sequence content

Per base sequence content

[WARN] Per base GC content

Per base GC content graph

[WARN] Per sequence GC content

Per sequence GC content graph

[OK] Per base N content

N content graph

[OK] Sequence Length Distribution

Sequence length distribution

[FAIL] Sequence Duplication Levels

Duplication level graph

[FAIL] Overrepresented sequences

Sequence Count Percentage Possible Source
ACCCAATTGGTCAAGGAAGCTTCTCTGACGGTATGCCTTTAGGAATCTCT 217386 1.499200041820306 No Hit
CTGGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTT 119965 0.8273372389067052 No Hit
GGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAAC 99155 0.6838213139148448 No Hit
GTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTC 82176 0.5667258362388814 No Hit
GGTACTTTCAACTTCATGATTGTGTTCCAAGCTGAACACAACATCCTTAT 77031 0.5312434030777511 No Hit
TGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTT 72748 0.5017057429749092 No Hit
CTGACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTG 71025 0.48982309334679897 No Hit
ACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCCTTGG 68198 0.47032672045146073 No Hit
GCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGGAAGCTTCTCTGA 62460 0.43075466962958203 No Hit
GTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTT 60157 0.4148720566907903 No Hit
CCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTA 46230 0.31882466181517094 No Hit
AGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAG 43818 0.30219033163350983 No Hit
GGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTC 43816 0.30217653865657645 No Hit
GCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGA 41214 0.28423187566624386 No Hit
ATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAACTACTGAGAA 39382 0.2715975087952642 No Hit
CCACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTA 39057 0.26935615004358926 No Hit
CCTGTTGCGGCTGCTACTGCTGTTTTCTTGATCTACCCAATTGGTCAAGG 37465 0.2583769404046156 No Hit
GAACACAACATCCTTATGCACCCATTCCACATGCTTGGTGTAGCTGGTGT 33500 0.2310323636341818 No Hit
GACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCT 32120 0.2215152095501469 No Hit
GCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTAT 31532 0.217460074331732 No Hit
CTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGA 30707 0.21177047134671112 No Hit
TGCTACATGGGTCGTGAGTGGGAACTTAGCTACCGTTTAGGTATGCGTCC 30333 0.20919118466016828 No Hit
TGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGC 30094 0.2075429239166289 No Hit
ATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTAT 30061 0.20731533979722805 No Hit
GCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATAT 29464 0.20319813618261293 No Hit
ACATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATG 29414 0.20285331175927834 No Hit
AGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCCT 27282 0.18814999834829102 No Hit
GACGGTATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTT 27088 0.18681207958575277 No Hit
AAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGCC 26936 0.1857638133388156 No Hit
CTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTA 26039 0.17957766318419285 No Hit
ACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTCTGCGACTG 24918 0.17184669961303115 No Hit
GCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGCATG 24861 0.17145359977042968 No Hit
ATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCCTCCTG 24171 0.16669502272841222 No Hit
CAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCGC 24157 0.16659847188987853 No Hit
CCTCCTGTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCT 23140 0.15958474311925278 No Hit
GGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGGAAACAACATCAT 22439 0.1547503047041017 No Hit
TTACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAA 22347 0.154115827765166 No Hit
GGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGCACTATGGCT 21244 0.14650900098640474 No Hit
ATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGTGAAA 20606 0.14210904134465524 No Hit
CCCCGGTTGTTACCCGACTACCAATAGGGGTGCTTCGTGCACCCCCAGAA 20442 0.14097801723611778 No Hit
GATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAACTTCTGTA 20371 0.14048836655498265 No Hit
GTATTTATTATTGCTTTCATTGCAGCTCCTCCTGTAGATATTGATGGTAT 19930 0.13744701514117147 No Hit
CCACGTAGTTGGTTAGCTACTTCTCACTTTGTATTAGGATTTTTCTTCTT 19539 0.13475048815069487 No Hit
TGACCAAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 18922 0.13049535476674592 No Hit
GCAAGCCTATGGGGTCGCTTCTGCGACTGGGTTACCAGCACTGAAAACCG 18767 0.12942639905440867 No Hit
GTAGATATTGATGGTATCCGTGAGCCTGTTTCTGGTTCTCTTCTTTACGG 17871 0.12324714538825264 No Hit
TTCATGCGTTGGAGAGACCGTTTCTTATTCTGTGCAGAAGCTATTTACAA 17695 0.12203336341811484 No Hit
GGCTCTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAAT 17631 0.12159198815624656 No Hit
ACTCTATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGCTCC 17432 0.12021958695137486 No Hit
TAGCTGCTTGGCCTGTAGTAGGTATCTGGTTCACTGCATTAGGTATCAGC 17015 0.1173437512607643 No Hit
ATGCCTTTAGGAATCTCTGGTACTTTCAACTTCATGATTGTGTTCCAAGC 16926 0.1167299637872287 No Hit
CATGCTTGGTGTAGCTGGTGTATTCGGTGGCTCTCTATTCAGTGCTATGC 16902 0.1165644480640281 No Hit
CTCTATTCAGTGCTATGCATGGTTCCTTAGTAACTTCAAGTTTAATCCGT 16636 0.11472998213188804 No Hit
TGACTGCTACTTTAGAAAGACGCGAAAGCGCAAGCCTATGGGGTCGCTTC 16380 0.11296448108441487 No Hit
TACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGA 16206 0.11176449209121048 No Hit
GCCCTAGGTAACTTCTGTATTATAGTTGCTCCAGTTTTAAACTGAAAAAA 15997 0.11032312600167185 No Hit
CCTACTCTATTAACTGCAACTTCTGTATTTATTATTGCTTTCATTGCAGC 15909 0.10971623501660295 No Hit
TACATCGGATGGTTCGGTGTTTTAATGATTCCTACTCTATTAACTGCAAC 15714 0.10837141976559801 No Hit
GCGACTGGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGT 15375 0.10603351017538942 No Hit
GCTCACGGTTACTTTGGTAGATTAATTTTCCAATACGCTAGCTTTAACAA 14982 0.10332319020797946 No Hit
GGGTTACCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTA 14671 0.10117838229483826 No Hit
CCAGCACTGAAAACCGCCTTTACATCGGATGGTTCGGTGTTTTAATGATT 14651 0.10104045252550442 No Hit
TGACCGAACTTGTAACCTGCGTTAGCAGACTCATTCTCAGTAGTTTCACG 14619 0.10081976489457027 No Hit

[WARN] Kmer Content

Kmer graph

Sequence Count Obs/Exp Overall Obs/Exp Max Max Obs/Exp Position
CTGCT 4438660 3.3979478 7.2027373 5
ACTTC 4728885 3.3552454 6.885556 60-64
AAAAA 4827045 3.2293375 4.370754 70-74
GGAAG 3709990 3.117473 5.9616947 15-19
AACTT 5366895 3.1096413 6.5029054 7
AGCTG 3813105 3.0582724 7.4101872 7
AGCAC 2793405 3.0235832 6.592618 80-84
GAAGA 3784475 2.947381 5.8191805 90-94
CTACT 3913355 2.7766094 10.040533 2
CTGGT 4100555 2.765849 12.851613 1
TCAAG 3689395 2.7425416 5.1766663 10-14
TCTCT 4568935 2.7262704 8.207403 20-24
CTTCA 3770450 2.6752152 7.2093587 60-64
CTTCT 4410795 2.631909 5.45937 20-24
TTCAA 4456580 2.5821943 8.239028 9
CAACT 3039235 2.564138 9.715894 9
CGGAA 2684905 2.5605774 7.146788 95-97
GCTGC 2570510 2.5246143 7.163992 3
CTGAA 3394530 2.5233512 6.271562 80-84
TGGTG 4222395 2.5093756 7.8975906 6
ACAAC 2498640 2.5066423 7.1817307 85-89
GGTGT 4161830 2.4733815 9.665351 1
AGGAA 3166335 2.4659684 6.052701 40-44
CAAGC 2264015 2.450571 7.3336854 75-79
CGGTG 2808595 2.4304452 10.898954 9
GCAGC 2069575 2.4169545 5.6661983 1
CAACA 2403900 2.411599 6.925994 85-89
GGTGG 3146570 2.3991368 9.505014 7
ATGCA 3158210 2.347681 6.131789 1
AGAGC 2456940 2.3431685 8.718144 95-97
CCGTG 2384640 2.342063 5.977049 60-64
TATGC 3733835 2.3342192 6.8511972 30-34
ACCCA 1885485 2.3162751 32.185753 1
ACATC 2714685 2.290322 5.6978498 90-94
GAAGC 2377335 2.2672498 6.7473984 15-19
AAGCT 3040930 2.2605 5.19008 15-19
TCTGC 2945145 2.2546105 5.556649 85-89
TCAAC 2663205 2.2468894 7.8198214 8
CAGCA 2060270 2.2300375 5.3675776 2
AAGAG 2859775 2.227217 5.0782237 90-94
CCAAG 2056255 2.2256916 6.977578 75-79
CGTGA 2768755 2.2206593 7.218518 9
TCGGA 2755630 2.2101324 5.745554 85-89
ATCGG 2755050 2.2096674 5.688463 95-97
CATGG 2727340 2.1874425 9.310423 2
GCTGG 2479385 2.1455598 8.071913 4
ACTGC 2324420 2.1158798 6.978711 4
GAGCA 2215715 2.1131136 5.9166417 90-94
TCCTT 3501090 2.0890903 5.6811767 40-44
GATCG 2562835 2.0555027 6.2173696 95-97
CAAGG 2144210 2.0449197 6.4297705 10-14
GCACA 1873165 2.0275147 6.4197173 95-97
CTCTG 2637980 2.019465 6.5819545 20-24
GGCTC 1998130 1.962454 5.1285944 10-14
CTTGG 2896785 1.953899 6.045837 2
CCTTA 2737640 1.9424144 6.839268 95-97
AAGGA 2486655 1.9366279 5.8019857 10-14
GCTAC 2108770 1.9195775 11.821535 1
CACAC 1547905 1.9015658 7.2982097 95-97
TGGCT 2817515 1.9004309 7.374328 9
TAGCT 3017140 1.886175 5.4322147 6
TTCCA 2654140 1.8831693 5.2381544 75-79
CATCC 1816845 1.8770392 6.7567782 90-94
AGATC 2524790 1.8768231 5.8377852 95-97
CCAGC 1410355 1.8693677 6.306226 1
TTTCA 3833900 1.8681704 7.1691265 8
TATTC 3810725 1.8568777 5.9125705 2
CACAA 1824585 1.8304286 6.296093 85-89
GTGGC 2112745 1.8282844 8.813206 8
TTCGG 2708060 1.8266028 8.406258 7
GCTTC 2370730 1.814876 7.104065 15-19
GCATG 2253065 1.807054 5.0777354 3
TGCTG 2673430 1.8032447 5.377789 6
CTCTA 2532335 1.796746 5.3955655 95-97
CCTGT 2319910 1.7759714 7.7765856 1
CACCC 1179960 1.7750585 7.0295243 50-54
TCGGT 2613230 1.7626394 9.261307 8
TTGGT 3747345 1.7358795 15.132034 7
TGAAC 2323805 1.7274194 5.8884535 80-84
GCACC 1300945 1.7243493 6.4253664 50-54
TGTAG 3126480 1.7221187 6.334148 3
GCAGG 1666920 1.7152326 5.163533 90-94
GTGTA 3097850 1.706349 8.809146 2
CATGC 1835580 1.6708971 5.9813757 2
CTTTC 2798585 1.6699077 8.358984 7
CCACA 1342285 1.6489662 7.016866 1
TGGTA 2941220 1.6200745 9.548293 2
CCCAA 1317385 1.6183772 31.635456 2
GTAGC 2009510 1.6117126 6.8658314 5
GCTTG 2366120 1.5959623 5.6835246 1
GAACA 1804125 1.59469 5.8496046 80-84
GTCTG 2336825 1.5762026 6.013518 90-94
CGCAG 1323360 1.5454868 5.9831705 90-94
TACTT 3168440 1.5439072 7.4856157 5
ACACA 1538915 1.5438436 6.0364637 85-89
GCTGA 1924375 1.5434306 5.178517 80-84
TCCAA 1827410 1.541747 5.361682 75-79
CCGCC 943720 1.5317496 5.44996 45-49
CCAAT 1806025 1.5237049 22.0966 3
GCAAC 1390150 1.5046992 5.7920938 1
AACAC 1481005 1.4857483 6.1033177 85-89
ATGCC 1623420 1.4777716 7.2576404 9
ATCTC 2044190 1.4503969 5.1080585 45-49
ACACG 1336275 1.4463848 6.2620893 95-97
GAGCC 1230610 1.4371686 5.0906487 95-97
ACGGT 1777065 1.4252815 6.9303713 4
GTATT 3298100 1.4159908 5.6797576 4
ACATG 1885470 1.4015794 8.963619 1
GGTAC 1699580 1.3631355 11.300488 3
ACTTT 2796530 1.3626839 7.4249363 6
GCTGT 2019160 1.3619356 5.4716372 7
GGTCA 1666300 1.3364435 21.02764 9
GACGG 1293540 1.3310307 8.700793 3
TGGTC 1892030 1.2761856 17.93765 8
TGTAT 2950660 1.2668225 5.494523 3
AACGC 1150415 1.2452098 5.14837 95-97
ATGGG 1761305 1.2446663 7.549874 3
GCGGC 983810 1.2396464 5.680081 7
ATTCG 1964035 1.227823 6.8808064 6
CAATT 2033860 1.1784421 15.299112 4
GGTCG 1359785 1.1767032 7.0827727 6
CTGAC 1282145 1.1671147 8.003852 1
TGGGT 1951145 1.1595683 5.7814994 4
GTACT 1834955 1.1471282 8.77091 4
TGACG 1425520 1.1433277 6.741454 2
ATTGG 1979910 1.0905684 14.650416 6
ACTTG 1724605 1.0781425 6.3610797 8
GGGTC 1221330 1.0568898 7.0458293 5
TGGCC 1037640 1.0191132 8.306229 1
TCGTG 1445185 0.9747858 5.5958896 8
CGGTA 1169970 0.9383657 6.487917 5
AATTG 1817960 0.92809516 12.551376 5
CTTGT 1575770 0.8284528 5.2468705 9
GTCGT 980920 0.6616365 5.342481 7
CCCCG 385180 0.6251847 5.0225773 1
TGACC 668485 0.60851055 5.909885 1
Produced by FastQC (version 0.10.1)